Stem-loop cel-mir-354

Accession

MI0000753

Description

Caenorhabditis elegans cel-mir-354 precursor miRNA mir-354

Gene
family

RF00799; mir-354

Literature search

4 open access papers mention cel-mir-354
(12 sentences)

Sequence

1153 reads, 57.0 reads per million, 67 experiments

cagagccgacuaagcaccuuGGUGCGGCUGCAGACGGGUAUccggcucgacguucauacaucacgacuucuuuccuuuuaccuuguuuguuGCUGCUCCUAUUGGUUUUG
(((((((((...........((.(((((.(((((((((((...((...((.(((..........))).))...))...)))).))))))).))))).))..)))))))))

Structure

         cuaagcaccuu  U     U       -    Ucc  cuc  c   caua 
cagagccga           GG GCGGC GCAGACG GGUA   gg   ga guu    c
|||||||||           || ||||| ||||||| ||||   ||   || |||     
GUUUUGGUU           CC CGUCG uguuugu ccau   cc   cu cag    a
         ---------AU  U     u       u    uuu  uuu  u   cacu 

Annotation confidence Low

Comments

This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing. The 5' end of the mature sequence ws mapped by PCR, but the 3' ends have not been experimentally determined.

Genome context

chrI: 14468382-14468491 [-]

Mature cel-miR-354-5p

• Accession: MIMAT0031894
• Description: Caenorhabditis elegans cel-miR-354-5p mature miRNA
• Sequence: 21 - GGUGCGGCUGCAGACGGGUAU - 41
• Evidence: not_experimental

Mature cel-miR-354-3p

• Accession: MIMAT0000696
• Description: Caenorhabditis elegans cel-miR-354-3p mature miRNA
• Sequence: 92 - GCUGCUCCUAUUGGUUUUG - 110
• Evidence: experimental ; cloned [1], PCR [1]

References

1. PubMed ID: 15317971
   Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
   "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
   "RNA (2004) 10:1309-1322