Stem-loop cel-mir-356a

Accession

MI0000755

Description

Caenorhabditis elegans cel-mir-356a precursor miRNA

Literature search

2 open access papers mention cel-mir-356a
(3 sentences)

Sequence

1178 reads, 7.0 reads per million, 28 experiments

uaaaguuuccuugagaacaaUGUGGUUGAGCAACGCGAACAAAucaucaauguaaauuucagcggAUUCGUUACGACGUGUCUCACCAcaucguuaguaggauuucagaa
.......((((....(((.(((((((.(((..((((((((.((((.....((.......))...)))).))).)).)))..)))))))))).)))...))))........

Structure

-uaaaguu    ugag   a       U   CA   -  -   A    aucaa  ua 
        uccu    aac aUGUGGU GAG  ACG CG AAC AAuc     ug  a
        ||||    ||| ||||||| |||  ||| || ||| ||||     ||  a
        agga    uug uacACCA CUC  UGC GC UUG UUAg     ac  u
aagacuuu    -uga   c       -   UG   A  A   C    --gcg  uu 

Annotation confidence Not enough data

Comments

This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing. The 5' end of the mature sequence ws mapped by PCR, but the 3' ends have not been experimentally determined.

Genome context

chrIII: 7860147-7860256 [+]

Clustered miRNAs

1 other miRNA is < 10 kb from cel-mir-356a

Mature cel-miR-356a-5p

• Accession: MIMAT0053605
• Description: Caenorhabditis elegans cel-miR-356a-5p mature miRNA
• Sequence: 21 - UGUGGUUGAGCAACGCGAACAAA - 43
• Evidence: experimental ; cloned [1], PCR [1]

Mature cel-miR-356a-3p

• Accession: MIMAT0000698
• Description: Caenorhabditis elegans cel-miR-356a-3p mature miRNA
• Sequence: 66 - AUUCGUUACGACGUGUCUCACCA - 88
• Evidence: experimental ; cloned [1], PCR [1]

References

1. PubMed ID: 15317971
   Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
   "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
   "RNA (2004) 10:1309-1322