Accession
MI0002074
Description
Danio rerio
dre-mir-454a precursor miRNA mir-454
Gene
family?
family?
RF00746;
mir-454
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
Dre-mir-454a is a candidate endogenous reference gene that was investigated for its suitability in a study [PMC8613261]. Normfinder software ranked dre-mir-454a as the third most stable gene among the preselected candidates [PMC8613261]. However, when expression data from MTZ treatments was considered, dre-mir-454a moved to the seventh position in the ranking [PMC8613261]. Additionally, when looking at three different strains, dre-mir-454a ranked fourth among the candidates [PMC8613261]. These rankings indicate that dre-mir-454a may not be as stable as other candidate reference genes in certain experimental conditions. The study aimed to identify suitable endogenous reference genes and investigated a total of nine candidates, including dre-mir-454a, for their suitability [PMC8613261]. The findings suggest that while dre-mir-454a may not be the most stable reference gene in all conditions, it could still be considered as a potential reference gene depending on the specific experimental context. Further research is needed to validate its stability and suitability in different experimental settings.
Literature search
1 open access papers mention dre-mir-454a
(1 sentences)
(1 sentences)
Sequence
2725
reads,
167
reads per million, 11 experiments
uugcaggucguuuaagaauuaauccuaauucuugggacccuaucaguauugccucugcuguccacuguguucagagUAGUGCAAUAUUGCUAAUAGGGuuuuagguuuuagguuguaccuucag
.((.((((...............(((((..((((((((((((((((((((((..(((((.....(((....)))))))).)))))))))...)))))))))))))..)))))....)))).)).
.((.((((...............(((((..((((((((((((((((((((((..(((((.....(((....)))))))).)))))))))...)))))))))))))..)))))....)))).)).
Structure
u c cguuuaagaauuaau uu --- cu gucca u ug aggu ccuaa cuugggacccuau caguauugc cugcu cug g || |||| ||||| ||||||||||||| ||||||||| ||||| ||| ac ucca ggauu ggauuuuGGGAUA GUUAUAACG GAUga gac u g u -----------uguu uu AUC -U ----- u
Annotation confidence
Not enough data
Do you think this miRNA is real?
Genome context
chr10: 33376818-33376941 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from dre-mir-454a
| Name | Accession | Chromosome | Start | End | Strand | Confidence |
|---|
Database links
Mature dre-miR-454a
| Accession | MIMAT0001877 |
| Description | Danio rerio dre-miR-454a mature miRNA |
| Sequence | 77 - UAGUGCAAUAUUGCUAAUAGGG - 98 |
| Evidence |
experimental
cloned [1] |
| Database links |
|
| Predicted targets |
|
References
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