miRBase entry: ppy-mir-15b

Stem-loop ppy-mir-15b

Accession

MI0002494

Description

Pongo pygmaeus ppy-mir-15b precursor miRNA

Sequence

uugaggccuuaaaguacugUAGCAGCACAUCAUGGUUUACAugcuacagucaagaugcgaaucauuauuugcugcucuagaaauuuaaggaaauucau
.((((.(((((((...(((.(((((((.((.(((((((.(((.((.......))))).))))))).)).))))))).)))...)))))))...)))).

Structure

u    --g       gua   U       C  C       A   g  ac 
 ugag   ccuuaaa   cug AGCAGCA AU AUGGUUU CAu cu  a
 ||||   |||||||   ||| ||||||| || ||||||| ||| ||  g
 acuu   ggaauuu   gau ucgucgu ua uacuaag gua ga  u
u    aaa       aaa   c       u  u       c   -  ac

Annotation confidence Not enough data

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Comments

Berezikov et al. used primers designed from human miRNA gene flanking sequence to amplify miRNA precursor regions in primates [1]. The expression of the mature miRNA was not validated.

Genome context

chr3: 163674098-163674195 [+]

Clustered miRNAs

1 other miRNA is < 10 kb from ppy-mir-15b

Name	Accession	Chromosome	Start	End	Strand	Confidence

Database links

Mature ppy-miR-15b

Accession	MIMAT0002205
Description	Pongo pygmaeus ppy-miR-15b mature miRNA
Sequence	20 - UAGCAGCACAUCAUGGUUUACA - 41
Evidence	not_experimental

References

PubMed ID: 15652478
Phylogenetic shadowing and computational identification of human microRNA genes
"Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E"
"Cell (2005) 120:21-24