miRBase entry: ppy-mir-27a

Stem-loop ppy-mir-27a


Accession
MI0003046
Description
Pongo pygmaeus ppy-mir-27a precursor miRNA


Sequence

cugaggagcacggggcuuagcugcuugugagcaggguccacaccaaguugugUUCACAGUGGCUAAGUUCCGCCccccag
(((.((.(..(((((((((((((((.(((((((.((.(........))).))))))))))))))))))))))..))))))

Structure
   a  a ca               u       g  u cac 
cug gg g  cggggcuuagcugcu gugagca gg c   a
||| || |  ||||||||||||||| ||||||| || |    
gac cc c  GCCUUGAAUCGGUGA CACUUgu uu g   c
   -  - CC               -       g  - aac 


Annotation confidence Not enough data
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Comments
Berezikov et al. used primers designed from human miRNA gene flanking sequence to amplify miRNA precursor regions in primates [1]. The expression of the mature miRNA was not validated.

Genome context
NW_002966782.1: 23150-23229 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from ppy-mir-27a
Name Accession Chromosome Start End Strand Confidence




Database links

Mature ppy-miR-27a

Accession MIMAT0002748
Description Pongo pygmaeus ppy-miR-27a mature miRNA
Sequence 53 - UUCACAGUGGCUAAGUUCCGCC - 74
Evidence not_experimental

References

  1. PubMed ID: 15652478
    Phylogenetic shadowing and computational identification of human microRNA genes
    "Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E"
    "Cell (2005) 120:21-24