MIR627 is a microRNA whose expression is influenced by the cellular environment, particularly under hypoxic conditions, as observed in hepatocellular carcinoma (HCC) cells [PMC8317525]. Research using ChIP assays with an H3K9ac antibody revealed that hypoxia leads to a decrease in H3 acetylation at the MIR627 promoter region, which correlates with the downregulation of MIR627 [PMC8317525]. This downregulation of MIR627 under hypoxia is dependent on HIF-1α; however, it does not occur through direct binding of HIF-1α to hypoxia-response elements (HRE) in the MIR627 promoter [PMC8317525]. Additionally, it was found that knockdown of HDAC3, an enzyme involved in histone deacetylation, results in increased acetylation and expression of MIR627, suggesting that HDAC3 plays a critical role in the repression of miR-627-5p during hypoxic conditions [PMC8317525'>PMC8317525]. Despite potential binding sites for HIF-1α on the MIR627 promoter region being identified and tested using ChIP and luciferase assays, no direct transcriptional activation by HIF-1α through these sites was observed [PMC8317525]. This indicates that miR-627-5p repression is mediated by mechanisms other than direct interaction with its promoter's HRE sites during hypoxia [PMC8317525].
---------ua c U u u cuuauua ugguaGUGAGUCUC AAGAAAAGAGGAgg gg u ||||||| |||||||||||||| |||||||||||||| || gaauaau accaUCACUCAGAG UUCUUUUCUccucc uu g aucaucauaaa a U u u
Disease | Description | Category | PubMed ID |
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Accession | MIMAT0003296 |
Description | Homo sapiens hsa-miR-627-5p mature miRNA |
Sequence | 16 - GUGAGUCUCUAAGAAAAGAGGA - 37 |
Evidence |
experimental
SAGE [1], cloned [2] |
Database links |
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Predicted targets |
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Accession | MIMAT0026623 |
Description | Homo sapiens hsa-miR-627-3p mature miRNA |
Sequence | 55 - UCUUUUCUUUGAGACUCACU - 74 |
Evidence |
experimental
Illumina [3] |
Database links |
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Predicted targets |
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