Accession
MI0005458
Description
Bos taurus
bta-mir-15a precursor miRNA
Gene family
MIPF0000006;
mir-15
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
bta-mir-15a is one of the 12 DA miRNAs identified in X and Y sperm [PMC7505075]. It is also one of the 123 mastitis-related miRNAs that are upregulated in cows with mastitis [PMC6107498]. The expression levels of bta-mir-15a and -15b are not significantly different between BLV-infected and uninfected cattle [PMC8432782]. In addition, bta-mir-15a is down-regulated in normalised data but not in un-normalised data [PMC3589390]. It has been suggested that bta-mir-15a may play a role in promoting lactation in mammary epithelial cells of dairy cows [PMC6268530]. The transfection of bta-mir-15a mimics/inhibitor into mammary epithelial cells has been shown to affect the expression of GHR protein and the synthesis of casein, but the mechanisms are still unknown [PMC6268530]. The regulatory relationship between bta-mir-15a and GHR needs to be confirmed [PMC6268530]. Transfection with bta-mir-15a mimics/inhibitor has been done using siPORT NeoFXTM Transfection Agent, with a final RNA concentration of 30 nM for bta-mir-15a mimics/inhibitor/negative control [PMC6268530'>PMC6268530'>PMC6268530]. Bovine miR-15a (bta-mir-15a) is located on bovine chromosome 12 between 18887743bp and 18887825bp [PMC6268530]. Bovine mammary epithelial cells transfected with bta-mir-15a mimics show reduced expression levels of GHR protein and casein, as well as decreased cell viability, while transfection with a bta-mir-15a inhibitor increases cell viability [PMC6268530]. The expression of GHR mRNA and protein is negatively regulated by bta-mir-15a [PMC6268530]. The expression of bta-mir-15a is regulated by DNA methylation and is positively correlated with the expression of its target gene CD163 [PMC6993440].
Literature search
17 open access papers mention bta-mir-15a
(31 sentences)
(31 sentences)
Sequence
20653
reads,
355
reads per million, 74 experiments
ccuuggaguaaagUAGCAGCACAUAAUGGUUUGUggauuuugaaaaggugcaggccauauugugcugccucaaaaauacaagg
(((((.......(..((((((((..((((((((((.............))))))))))..))))))))..).......)))))
(((((.......(..((((((((..((((((((((.............))))))))))..))))))))..).......)))))
Structure
gaguaaa UA UA gauuu
ccuug g GCAGCACA AUGGUUUGUg u
||||| | |||||||| |||||||||| g
ggaac c cgucgugu uaccggacgu a
auaaaaa uc ua ggaaa
Annotation confidence
Not enough data
Do you think this miRNA is real?
Genome context
chr12: 19660532-19660614 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from bta-mir-15a
| Name | Accession | Chromosome | Start | End | Strand | Confidence |
|---|
Database links
Mature bta-miR-15a
| Accession | MIMAT0004334 |
| Description | Bos taurus bta-miR-15a mature miRNA |
| Sequence | 14 - UAGCAGCACAUAAUGGUUUGU - 34 |
| Evidence |
experimental
cloned [1] |
References
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