Stem-loop hsa-mir-301b

Accession

MI0005568

Symbol

HGNC: MIR301B

Description

Homo sapiens hsa-mir-301b precursor miRNA

Summary

Caution, this is an AI generated summary based on literature. This may have errors.

MIR301B is a microRNA, a small non-coding RNA molecule, which plays a role in the regulation of gene expression [PMC8109121]. Its expression, along with other microRNAs such as miR-454, miR301a, miR130a, and miR130b, was quantified using the 2-ΔΔCt method with normalization to U6 [PMC8109121]. The study of these microRNAs led to further investigation into the influence of BHLHE40/41 on the expression levels of MIR130B and MIR301B [PMC6659797]. This suggests that BHLHE40/41 may be a regulatory factor affecting MIR301B expression [PMC6659797].

Literature search

62 open access papers mention hsa-mir-301b
(167 sentences)

Sequence

38694 reads, 581 reads per million, 84 experiments

gccgcagguGCUCUGACGAGGUUGCACUACUgugcucugagaagCAGUGCAAUGAUAUUGUCAAAGCaucugggacca
.((.((((((((.((((((.((((((((.((...........)).))))))))....)))))).))))))))))....

Structure

---g  g        C      ---G        A  gugc 
    cc cagguGCU UGACGA    GUUGCACU CU    u
    || |||||||| ||||||    |||||||| ||    c
    gg gucuaCGA ACUGUU    UAACGUGA ga    u
acca  -        A      AUAG        C  agag 

Annotation confidence High

Comments

This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques [1]. Expression was later confirmed by cloning [2].

Genome context

chr22: 21652981-21653058 [+]

Clustered miRNAs

1 other miRNA is < 10 kb from hsa-mir-301b

Mature hsa-miR-301b-3p

• Accession: MIMAT0004958
• Description: Homo sapiens hsa-miR-301b-3p mature miRNA
• Sequence: 45 - CAGUGCAAUGAUAUUGUCAAAGC - 67
• Evidence: experimental ; cloned [2]

Mature hsa-miR-301b-5p

• Accession: MIMAT0032026
• Description: Homo sapiens hsa-miR-301b-5p mature miRNA
• Sequence: 10 - GCUCUGACGAGGUUGCACUACU - 31
• Evidence: not_experimental

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 16954537
   Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis
   "Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E"
   "Genome Res (2006) 16:1289-1298