Stem-loop sequence osa-MIR166h

Accession MI0001143
Description Oryza sativa miR166h stem-loop
Gene family MIPF0000004; MIR166
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-166_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

The plant mir-166 microRNA precursor is a small non-coding RNA gene. This microRNA (miRNA) has now been predicted or experimentally confirmed in a wide range of plant species. microRNAs are transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. In this case the mature sequence comes from the 3' arm of the precursor, and both Arabidopsis thaliana and rice genomes contain a number of related miRNA precursors which give rise to almost identical mature sequences. The mature products are thought to have regulatory roles through complementarity to messenger RNA.

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Stem-loop
   gg                uu g    cu   g  a       cuuaau   ucu     ga 
5'   uggcuuguggggaaug  g cugg  cga gu uccacau      ucc   ccggc  u
     ||||||||||||||||  | ||||  ||| || |||||||      |||   |||||   
3'   gccgaacgcuccuuac  c gacc  gcu cg aggugug      ggg   ggccg  c
   ua                uu g    ag   g  a       -----c   cuc     ag 
Get sequence
Deep sequencing
1084035 reads, 3.22e+04 reads per million, 68 experiments
Confidence Annotation confidence: high
Feedback: Do you believe this miRNA is real?
Comments

This sequence is a predicted paralogue of the previously identified miR166 family [1]. It is predicted to target mRNAs coding for HD-Zip transcription factors.

Genome context
Coordinates (MSU7) Overlapping transcripts
Chr2: 32435174-32435292 [-]
intergenic
Clustered miRNAs
< 10kb from osa-MIR166h
osa-MIR166h Chr2: 32435174-32435292 [-]
osa-MIR166k Chr2: 32435003-32435129 [-]

Mature sequence osa-miR166h-5p

Accession MIMAT0022882
Sequence

13 - 

ggaauguuggcuggcucgagg

 - 33

Get sequence
Deep sequencing 176 reads, 19 experiments
Evidence experimental; Illumina [2]

Mature sequence osa-miR166h-3p

Accession MIMAT0001073
Previous IDs osa-miR166h
Sequence

89 - 

ucggaccaggcuucauuccuc

 - 109

Get sequence
Deep sequencing 103636 reads, 68 experiments
Evidence by similarity; MI0000201

References

1
2
PMID:21901091 "Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors" Du P, Wu J, Zhang J, Zhao S, Zheng H, Gao G, Wei L, Li Y PLoS Pathog. 7:e1002176(2011).