miRBase entry: dme-mir-286

Stem-loop dme-mir-286


Accession
MI0000380
Description
Drosophila melanogaster dme-mir-286 precursor miRNA
Gene family
MIPF0000225; mir-286

Literature search
8 open access papers mention dme-mir-286
(27 sentences)

Sequence

119660 reads, 5368 reads per million, 49 experiments
uuaaaauugaauGGCGAAUGUCGGUAUGGUCUCUuuuucaaagaaagguuucgauuaagcgaagUGACUAGACCGAACACUCGUGCUauaauuuuaaaau
(((((((((....((((.(((((((.(((((.((((......(((....)))........)))).))))).)))).))).))))....)))))))))...

Structure
---         aauG    A   -    A     U    --uucaaa   a 
   uuaaaauug    GCGA UGU CGGU UGGUC CUuu        gaa g
   |||||||||    |||| ||| |||| ||||| ||||        |||  
   aauuuuaau    UGCU ACA GCCA AUCAG gaag        cuu g
uaa         aUCG    C   A    G     U    cgaauuag   u 


Annotation confidence High
Do you think this miRNA is real?
Comments
miR-286 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 22-24 nt with 23 nt the most commonly expressed.

Genome context
chr2R: 19661422-19661521 [-]
Clustered miRNAs
7 other miRNAs are < 10 kb from dme-mir-286
Name Accession Chromosome Start End Strand Confidence




Database links

Mature dme-miR-286-3p

Accession MIMAT0000359
Description Drosophila melanogaster dme-miR-286-3p mature miRNA
Sequence 65 - UGACUAGACCGAACACUCGUGCU - 87
Evidence experimental
Northern [1], cloned [2], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-286-5p

Accession MIMAT0020820
Description Drosophila melanogaster dme-miR-286-5p mature miRNA
Sequence 13 - GGCGAAUGUCGGUAUGGUCUCU - 34
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42