miRBase entry: dme-mir-263b

Stem-loop dme-mir-263b


Accession
MI0000383
Description
Drosophila melanogaster dme-mir-263b precursor miRNA
Gene family
MIPF0000122; mir-263

Literature search
6 open access papers mention dme-mir-263b
(66 sentences)

Sequence

11247 reads, 551 reads per million, 44 experiments
uugcugacuuugaguCUUGGCACUGGGAGAAUUCACaguugacuuuauuauucuGUGGUUCUGCGGGUGCCAAAACuuaaaaaccggcuu
..((((..(((((((.((((((((.(.(((((.(((((..............)))))))))).).)))))))).)))))))...))))..

Structure
uu    -ac       C        G G     U     uugacu 
  gcug   uuugagu UUGGCACU G AGAAU CACag      u
  ||||   ||||||| |||||||| | ||||| |||||       
  cggc   aaauuCA AACCGUGG C UCUUG GUGuc      u
uu    caa       A        G G     -     uuauua 


Annotation confidence High
Do you think this miRNA is real?
Comments
miR-263b was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the ends of the excised miRNA by cloning.

Genome context
chr3L: 15843406-15843495 [-]

Database links

Mature dme-miR-263b-5p

Accession MIMAT0000362
Description Drosophila melanogaster dme-miR-263b-5p mature miRNA
Sequence 16 - CUUGGCACUGGGAGAAUUCAC - 36
Evidence experimental
cloned [2], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-263b-3p

Accession MIMAT0020822
Description Drosophila melanogaster dme-miR-263b-3p mature miRNA
Sequence 55 - GUGGUUCUGCGGGUGCCAAAAC - 76
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42