Stem-loop sequence mmu-mir-223

Accession

MI0000703 (change log)

Symbol

MGI:Mir223

Description

Mus musculus miR-223 stem-loop

Gene family

MIPF0000067; mir-223

Literature search

185 open access papers mention mmu-mir-223
(2034 sentences)

Stem-loop

   u    ccaucu   -gu        cc u              gaguug         u 
5'  cugg      gca   gucacgcu  g guauuugacaagcu      gacacucug g
    ||||      |||   ||||||||  | ||||||||||||||      ||||||||| u
3'  gacu      cgu   cgguguga  c cauaaacuguuuga      cugugagau g
   -    ---auc   acu        ac c              ------         g 

Deep sequencing

588071 reads, 1.7e+03 reads per million, 97 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Mouse mir-223 is predicted [2] based on homology to a reported miR from human (MI0000300) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chrX: 96242817-96242926 [+]
• Overlapping transcripts:

  sense

  OTTMUST00000044608 ; RP23-23B17.2-002; exon 2

  OTTMUST00000044607 ; RP23-23B17.2-001; exon 3

  ENSMUST00000147681 ; F630028O10Rik-002; exon 2

  ENSMUST00000133125 ; F630028O10Rik-001; exon 3

Database links

• EntrezGene:Mirn223
• MGI:Mir223
• RNAcentral:URS00006FEFE5_10090
• miRBase Tracker:MI0000703

Mature sequence mmu-miR-223-5p

• Accession: MIMAT0017056
• Previous IDs: mmu-miR-223*
• Sequence: 26 - cguguauuugacaagcugaguug - 48
• Deep sequencing: 13863 reads, 88 experiments
• Evidence: experimental; Illumina [5]
• Database links:
  • RNAcentral:URS00000D5CE2_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-223-5p
  • miRDB:mmu-miR-223-5p

Mature sequence mmu-miR-223-3p

• Accession: MIMAT0000665
• Previous IDs: mmu-miR-223
• Sequence: 68 - ugucaguuugucaaauacccca - 89
• Deep sequencing: 574187 reads, 96 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00000B7E30_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-223-3p
  • miRDB:mmu-miR-223-3p
  • microrna.org:mmu-miR-223-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).