Stem-loop sequence hsa-mir-99b

Accession

MI0000746 (change log)

Symbol

HGNC:MIR99B

Description

Homo sapiens miR-99b stem-loop

Gene family

MIPF0000033; mir-10

Literature search

137 open access papers mention hsa-mir-99b
(395 sentences)

Stem-loop

        cc         ac   c    c - -   c 
5' ggcac  acccguaga  cga cuug g g ggc u
   |||||  |||||||||  ||| |||| | | |||  
3' cugug  ugggugucu  gcu gaac c c ccg u
        cc         gu   c    a a g   c 

Deep sequencing

1421946 reads, 1.74e+03 reads per million, 159 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

This sequence is the predicted human homologue of mouse miR-99b, cloned from mouse brain [1] and embryonic stem cells [2,3]. Its expression was later validated in human [4-6].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr19: 51692612-51692681 [+]
• Overlapping transcripts:

  sense

  OTTHUMT00000465190 ; SIGLEC20P-001; intron 6

  ENST00000595243 ; SIGLEC20P-001; intron 6

Clustered miRNAs

< 10kb from hsa-mir-99b

• hsa-mir-99b: chr19: 51692612-51692681 [+]
• hsa-let-7e: chr19: 51692786-51692864 [+]
• hsa-mir-125a: chr19: 51693254-51693339 [+]

Database links

• EntrezGene:MIR99B
• HGNC:MIR99B
• RNAcentral:URS000075E904_9606
• miRBase Tracker:MI0000746

Mature sequence hsa-miR-99b-5p

• Accession: MIMAT0000689
• Previous IDs: hsa-miR-99b
• Sequence: 7 - cacccguagaaccgaccuugcg - 28
• Deep sequencing: 1402586 reads, 159 experiments
• Evidence: experimental; cloned [4-6]
• Database links:
  • RNAcentral:URS00002C10B3_9606
• Predicted targets:
  • TargetMiner:hsa-miR-99b-5p
  • TargetScanVert:hsa-miR-99b-5p
  • miRDB:hsa-miR-99b-5p
  • microrna.org:hsa-miR-99b-5p

Mature sequence hsa-miR-99b-3p

• Accession: MIMAT0004678
• Previous IDs: hsa-miR-99b*
• Sequence: 45 - caagcucgugucuguggguccg - 66
• Deep sequencing: 19359 reads, 149 experiments
• Evidence: experimental; cloned [5]
• Database links:
  • RNAcentral:URS00001C308D_9606
• Predicted targets:
  • TargetMiner:hsa-miR-99b-3p
  • TargetScanVert:hsa-miR-99b-3p
  • miRDB:hsa-miR-99b-3p
  • microrna.org:hsa-miR-99b-3p

References

1. PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2. PMID:12919684 "Embryonic stem cell-specific MicroRNAs" Houbaviy HB, Murray MF, Sharp PA Dev Cell. 5:351-358(2003).
3. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
4. PMID:15978578 "Identification of human fetal liver miRNAs by a novel method" Fu H, Tie Y, Xu C, Zhang Z, Zhu J, Shi Y, Jiang H, Sun Z, Zheng X FEBS Lett. 579:3849-3854(2005).
5. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
6. PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).