miRBase entry: cel-mir-358

Stem-loop cel-mir-358


Accession
MI0000757
Description
Caenorhabditis elegans cel-mir-358 precursor miRNA
Gene family
MIPF0000226; mir-358


Sequence

348 reads, 29 reads per million, 11 experiments
aaacucgcgacggcuggcgACCUGGCCAGGCAUUCCAACUGUcaaacuuacaaagcuuucucgacaAUUGGUAUCCCUGUCAAGGUCUcaauccguucagcgaauucguu
....(((((((((.(((.((((((((.(((.((.((((.((((...................)))).)))).)).)))))).))))))))..)))))..)))).......

Structure
---aaac    --     -c   c     -   C   C  U    C    aaacuuac 
       ucgc  gacgg  ugg gACCU GGC AGG AU CCAA UGUc        a
       ||||  |||||  ||| ||||| ||| ||| || |||| ||||        a
       agcg  uugcc  acU CUGGA CUG UCC UA GGUU acag        a
uugcuua    ac     ua   -     A   -   C  U    A    cucuuucg 


Annotation confidence High
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Comments
This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing [1]. The extents of the dominant mature miRNA species are adjusted here in accordance with a large scale cloning and sequencing study [2].

Genome context
chrV: 8580858-8580967 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from cel-mir-358
Name Accession Chromosome Start End Strand Confidence




Database links

Mature cel-miR-358-5p

Accession MIMAT0015120
Description Caenorhabditis elegans cel-miR-358-5p mature miRNA
Sequence 20 - ACCUGGCCAGGCAUUCCAACUGU - 42
Evidence experimental
CLIPseq [4], Illumina [5]
Database links

Mature cel-miR-358-3p

Accession MIMAT0000700
Description Caenorhabditis elegans cel-miR-358-3p mature miRNA
Sequence 67 - AUUGGUAUCCCUGUCAAGGUCU - 88
Evidence experimental
cloned [1-2], PCR [1], Illumina [3,5], CLIPseq [4]
Database links
Predicted targets

References

  1. PubMed ID: 17174894
    Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans
    "Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP"
    "Cell (2006) 127:1193-1207

  2. PubMed ID: 19460142
    Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development
    Kato M, de Lencastre A, Pincus Z, Slack FJ
    Genome Biol (2009) 10:R54

  3. PubMed ID: 20062054
    Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans
    "Zisoulis DG, Lovci MT, Wilbert ML, Hutt KR, Liang TY, Pasquinelli AE, Yeo GW"
    "Nat Struct Mol Biol (2010) 17:173-179

  4. PubMed ID: 15317971
    Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
    "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
    "RNA (2004) 10:1309-1322

  5. PubMed ID: 21307183
    Improved annotation of C. elegans microRNAs by deep sequencing reveals structures associated with processing by Drosha and Dicer
    "Warf MB, Johnson WE, Bass BL"
    "RNA (2011) 17:563-577