Stem-loop cel-mir-392

Accession

MI0000819

Description

Caenorhabditis elegans cel-mir-392 precursor miRNA

Gene family

MIPF0000372; mir-392

Sequence

253 reads, 37 reads per million, 13 experiments

uuuuugaaaaucucgcagacguguucagucAGCAUUCGUGGUUGAGGAUAUcgaacacaaaaaaagaUAUCAUCGAUCACGUGUGAUGAcagauuuucugcgacuaacag
............(((((((.....((.((((.(((.(((((((((.((((((.............)))))).))))))))).))).)))).))...))))))).......

Structure

uuuuugaaaauc       cgugu  a    G   U         G      gaaca 
            ucgcaga     uc gucA CAU CGUGGUUGA GAUAUc     c
            |||||||     || |||| ||| ||||||||| ||||||     a
            agcgucu     ag cAGU GUG GCACUAGCU CUAUag     a
-----gacaauc       --uuu  a    A   U         A      aaaaa 

Annotation confidence High

Comments

This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing [1]. The 5' end of the mature sequence was mapped by PCR, and the 3' end confirmed later [2].

Genome context

chrX: 13597231-13597340 [+]

Mature cel-miR-392-3p

• Accession: MIMAT0000768
• Description: Caenorhabditis elegans cel-miR-392-3p mature miRNA
• Sequence: 68 - UAUCAUCGAUCACGUGUGAUGA - 89
• Evidence: experimental ; cloned [1-2], PCR [1], Illumina [3-4]

Mature cel-miR-392-5p

• Accession: MIMAT0020344
• Description: Caenorhabditis elegans cel-miR-392-5p mature miRNA
• Sequence: 31 - AGCAUUCGUGGUUGAGGAUAU - 51
• Evidence: experimental ; Illumina [4]

References

1. PubMed ID: 17174894
   Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans
   "Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP"
   "Cell (2006) 127:1193-1207


2. PubMed ID: 19460142
   Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development
   Kato M, de Lencastre A, Pincus Z, Slack FJ
   Genome Biol (2009) 10:R54


3. PubMed ID: 15317971
   Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
   "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
   "RNA (2004) 10:1309-1322


4. PubMed ID: 21307183
   Improved annotation of C. elegans microRNAs by deep sequencing reveals structures associated with processing by Drosha and Dicer
   "Warf MB, Johnson WE, Bass BL"
   "RNA (2011) 17:563-577