Stem-loop sequence hsa-mir-374b

Accession

MI0005566 (change log)

Symbol

HGNC:MIR374B

Description

Homo sapiens miR-374b stem-loop

Gene family

MIPF0000288; mir-374

Literature search

60 open access papers mention hsa-mir-374b
(147 sentences)

Stem-loop

   --  u    ug au                     c 
5'   ac cgga  g  auaauacaaccugcuaagugu c
     || ||||  |  |||||||||||||||||||||  
3'   ug gccu  u  uauuauguuggacgauucacg u
   uc  u    gu ac                     a 

Deep sequencing

497877 reads, 1.11e+03 reads per million, 159 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques [1]. Expression was later confirmed by cloning [2].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chrX: 74218547-74218618 [-]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from hsa-mir-374b

• hsa-mir-374c: chrX: 74218549-74218618 [+]
• hsa-mir-374b: chrX: 74218547-74218618 [-]
• hsa-mir-421: chrX: 74218377-74218461 [-]

Database links

• EntrezGene:MIR374B
• HGNC:MIR374B
• RNAcentral:URS000075BF8B_9606
• miRBase Tracker:MI0005566

Mature sequence hsa-miR-374b-5p

• Accession: MIMAT0004955
• Previous IDs: hsa-miR-374b
• Sequence: 11 - auauaauacaaccugcuaagug - 32
• Deep sequencing: 491420 reads, 159 experiments
• Evidence: experimental; cloned [2-3]
• Database links:
  • RNAcentral:URS000033F45D_9606
• Predicted targets:
  • TargetMiner:hsa-miR-374b-5p
  • TargetScanVert:hsa-miR-374b-5p
  • miRDB:hsa-miR-374b-5p
  • microrna.org:hsa-miR-374b-5p

Mature sequence hsa-miR-374b-3p

• Accession: MIMAT0004956
• Previous IDs: hsa-miR-374b*
• Sequence: 41 - cuuagcagguuguauuaucauu - 62
• Deep sequencing: 6449 reads, 143 experiments
• Evidence: experimental; cloned [2]
• Database links:
  • RNAcentral:URS00001EFEC1_9606
• Predicted targets:
  • TargetMiner:hsa-miR-374b-3p
  • TargetScanVert:hsa-miR-374b-3p
  • miRDB:hsa-miR-374b-3p
  • microrna.org:hsa-miR-374b-3p

References

1. PMID:16954537 "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis" Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E Genome Res. 16:1289-1298(2006).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).