Stem-loop sequence bta-mir-3431

Accession

MI0014498 (change log)

Description

Bos taurus miR-3431 stem-loop

Gene family

MIPF0002046; mir-3431

Literature search

5 open access papers mention bta-mir-3431
(10 sentences)

Stem-loop

   auacccaa    u c     cc        -    g       -au   c 
5'         ugaa g gaaaa  ucagucag ccuu uggaugu   guu u
           |||| | |||||  |||||||| |||| |||||||   ||| g
3'         acuu c cuuuu  agucaguc ggag aucuaca   cag c
   ---aaucg    u a     aa        a    -       guc   a 

Deep sequencing

8333 reads, 4.41 reads per million, 68 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Jin et al. predicted this miRNA bioinformatically in [1], and validated its expression by qPCR in [2].

Genome context

• Coordinates (Btau_5.0.1; GCA_000003205.6): chrX: 34879430-34879526 [-]
• Overlapping transcripts:

  sense

  ENSBTAT00000065531 ; GABRE-201; intron 6

  ENSBTAT00000018017 ; GABRE-202; intron 6

Clustered miRNAs

< 10kb from bta-mir-3431

• bta-mir-452: chrX: 34882394-34882478 [-]
• bta-mir-224: chrX: 34881360-34881440 [-]
• bta-mir-3431: chrX: 34879430-34879526 [-]

Database links

• EntrezGene:MIR3431
• RNAcentral:URS0000759B46_9913
• miRBase Tracker:MI0014498

Mature sequence bta-miR-3431

• Accession: MIMAT0017394
• Sequence: 21 - ccucagucagccuuguggaugu - 42
• Deep sequencing: 5336 reads, 67 experiments
• Evidence: experimental; qPCR [2], Illumina [3]
• Predicted targets:
  • TargetScanVert:bta-miR-3431

References

1. PMID:19758457 "Characterization of bovine miRNAs by sequencing and bioinformatics analysis" Jin W, Grant JR, Stothard P, Moore SS, Guan LL BMC Mol Biol. 10:90(2009).
2. PMID:20423511 "Characterization of microRNA expression in bovine adipose tissues: a potential regulatory mechanism of subcutaneous adipose tissue development" Jin W, Dodson MV, Moore SS, Basarab JA, Guan LL BMC Mol Biol. 11:29(2010).
3. PMID:21912509 "Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein cattle" Huang J, Ju Z, Li Q, Hou Q, Wang C, Li J, Li R, Wang L, Sun T, Hang S, Gao Y, Hou M, Zhong J Int J Biol Sci. 7:1016-1026(2011).