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13 publications mentioning dme-mir-281-2

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-281-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 22
Other miRNAs from this paper: dme-mir-1, mmu-mir-1a-1, dme-mir-281-1, mmu-mir-1a-2, mmu-mir-1b
Here we performed a series of gene expression analyses, including of both mRNAs and microRNAs (miRNAs), and identified a number of mRNAs and miRNAs (miRNA-1 and miRNA-281) with circadian rhythm -dependent altered expression in dfmr1 mutant flies. [score:5]
Here we performed a series of gene expression analyses, including of both mRNAs and microRNAs (miRNAs), and identified a number of mRNAs and two miRNAs (miRNA-1 and miRNA-281) with circadian rhythm -dependent altered expression in dfmr1 mutant flies. [score:5]
The loss of dFmr1 leads to altered expression and biogenesis of miR-1 and miR-281. [score:3]
In particular, the loss of dFmr1 led to the circadian rhythm -dependent alteration of miR-1 and miR-281 expression. [score:3]
Interestingly, while the ratio between CT00 and CT12 in w [1118] is unchanged, both miR-1 and miR-281 are significantly elevated at CT00, which displayed circadian rhythm -dependent expression in dfmr1 mutant fly. [score:3]
Given that dFMR1 is also found to be associated with Dicer, we further examined the processing of both miR-1 and miR-281 by real-time RT-PCR. [score:1]
We found that the precursor forms, but not the pri-form, of both miR-1 and miR-281 increased at CT00, indicating that the processing of these two miRNAs is altered in a circadian rhythm -dependent manner in the absence of dFmr1. [score:1]
Among these miRNAs, we saw significant changes of mature miR-1 and miR-281, in particular at CT00 (Figure 4A and 4B). [score:1]
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2
[+] score: 10
Other miRNAs from this paper: dme-mir-281-1
In this paper, we report a series of efficient applications derived from HDR -mediated genomic modifications by TALEN and CRISPR/Cas9 in manipulating the Drosophila genome to precisely: (1) generate deletions of the micro RNAs, specifically, miR-281; (2) make genomic replacement of endogenous sequences of CG4221, chameau and CG5961 genes with exogenous loxP sites or restriction enzyme cutting sites of SmaI and HindIII, respectively; and (3) insert coding sequences of GFP and Myc to tag the Chameau and CG4221 proteins for tracing their endogenous expressions. [score:3]
Here, for the TALEN -mediated HDR mutagenesis, we selected two Drosophila genomic loci, miR-281 and chameau. [score:1]
miR-281 consists of two adjacent miRNAs, pre-miR-281-1 and pre-miR-281-2 (Xiong et al., 2009), the functions of which remain unknown. [score:1]
Fig. 1. (A,E) TALEN -mediated miR-281 deletion and molecular identification. [score:1]
In the case of miR-281, a pair of TALENs (see figure legends and supplementary material Table S1 for details) was designed to generate a DSB within the miR-281 loci (Fig.  1A). [score:1]
One pair of homologous arms (HAs) was selected from the flanking genomic regions of the miR-281 loci (as indicated by HA-L, 1.3 kb, and HA-R, 1.9 kb, in Fig.  1A; supplementary material Table S3) and cloned into the pBSK vector to generate the donor plasmid that will be used to mediate the HDR. [score:1]
Independent transcription of miR-281 in the intron of ODA in Drosophila melanogaster. [score:1]
The statistics of both the precise deletion of miR-281 and precise DNA replacement in chameau are summarized in Table 1. Collectively, we have successfully generated two precise genomic modifications in Drosophila through TALEN induced DSBs and exogenous donor plasmids, which induced the HDR pathway. [score:1]
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3
[+] score: 3
Four microRNA families were affected by multiple matching reads: mir-983, mir-281, mir-276 and mir-2. The first three did not show any differential expression between sexes. [score:3]
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4
[+] score: 3
The 281 cluster, containing a tandem of identical and indistinguishable dme-mir-281-1 and dme-mir-281-2 was excluded from the further expression analyzes. [score:3]
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5
[+] score: 1
MiR-133, miR-219, miR-263a, miR-274, miR-281-2*, miR-282, miR-283 and miR-310 which are also collected in miRBase without cloning evidence, are also identified. [score:1]
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6
[+] score: 1
Finally, for another microRNA, miR-281, we make only a total of 34 predictions of which only 13 (38%) and 17 (50%) occur among the predictions of Stark et al. [25] and Grün et al. [23], respectively. [score:1]
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7
[+] score: 1
They are let-7, miR-281, miR-34, miR-12, and miR-306. [score:1]
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8
[+] score: 1
The miRs (sfr-mir-305-5p, sfr-mir-307-3p, sfr-mir-71-3p, sfr-mir-281, sfr-mir-317, sfr-mir-2756, sfr-mir-932, sfr-mir-184-3p, sfr-mir-2766, Novel_miR15, Novel_miR16, and Novel_miR17) were easily detectable in total RNA isolated from Sf21 cells (Fig. 4). [score:1]
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9
[+] score: 1
Some of the miRNAs (miR-8, miR-9a, miR-10, miR-71, miR-252, miR-276, miR-281) are represented by both strands,-3p and-5p, as was also observed in the previous N6 library [14]. [score:1]
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10
[+] score: 1
51  miR-1903893.672.796.69  miR-193324.613.342.6  miR-252736.2934.148.14  miR-263a2325.6119.746.7  miR-27536418.7410.2887.04  miR-3151512.151.5412.16  miR-3171213.792.639.02  miR-965391.581.792.46  miR-993697.194.116.07  miR-let7607546.7640.8818.64  miR-281-2*18854.113. [score:1]
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11
[+] score: 1
Comparisons between P. tepidariorum and D. melanogaster shows that mir-10, mir-993a, mir-278b, mir-281, also display changes in arm use (fig. 4 B). [score:1]
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12
[+] score: 1
gov/Genbank/) GeneIDs for the genes discussed in this paper are arf3 (817014), eve (36039), hb (41032), hoxb8 (15416), iab-4 (3772110), lbl1 (100037819), miR-10 (3772568), miR-11 (3771987), miR-196 (387191), miR-274 (3771876), miR-281–1 (3772402), miR-281–2 (3772497), miR-309 (3772613), scr (40833), tas3 (3768766), and ubx (42034). [score:1]
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13
[+] score: 1
Likewise, miR-281* in Cx. [score:1]
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