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18 publications mentioning dme-mir-100

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-100. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 50
For expression pattern studies and hormonal treatments, we selected the three miRNAs more highly expressed (M-value between 1.6 and 5.3) in the N5 library (miR-252-3p, miR-276-5p and miR-190-5p) and the four more differentially expressed (M-value between −1.4 and −2.0) in the N6 library (bantam-3p, miR-100-5p, miR-125-5p and let-7-5p). [score:7]
In the hemimetabolan B. germanica, the present study shows that expression of let-7, miR-100 and miR-125 concentrates in the last nymphal instar, having an expression burst around the peak of 20E that triggers metamorphosis (Figure  2). [score:5]
3. Hormonal treatments indicated that 20E increases the expression of let-7, miR-100 and miR-125, but inhibits that of miR-252-3p. [score:5]
indicate that miR-252-3p is the only clear representative of miRNA that is well expressed in N5 and with decreased expression in N6, whereas let-7-5p, miR-100-5p and miR-125-5p are clear representatives of the reverse pattern (Figure  2). [score:5]
However, the expression levels of miR-1-3p and miR-100-5p were significantly increased after treatment with 20E, whereas those of bantam-3p, miR-125-5p, miR-14-3p, miR-276-3p, miR-34-5p and let-7-5p showed a tendency to increase with respect to controls, although differences were not statistically significant. [score:3]
This is consistent with previous studies in D. melanogaster that revealed that 20E increases the levels of these miRNAs [30, 36] by inducing the expression of the polycistronic primary transcript that contains the let-7, miR-100 and miR-125 precursors [37]. [score:3]
Among those miRNAs that increased in expression after 20E treatment, we found let-7, miR-100 and miR-125. [score:3]
Also interesting are the patterns of let-7, miR-100 and miR-125, which show low expression in N5, which then increased in N6 reaching a burst around the days of the 20E peak (Figure  2); these patterns are also consistent with the sequencing data. [score:3]
Whereas the expression pattern of miR-252-3p had yet to be studied in other insects, those patterns of let-7, miR-100 and miR-125 have been thoroughly examined. [score:3]
Expression of let-7, miR-100 and miR-125 was increased by 20E, which has also been observed in D. melanogaster. [score:3]
The increase in expression of miR-100-5p after 20E treatment is statistically significant, whereas let-7-5p and miR-125-5p had a tendency to increase (Figure  3A). [score:3]
qRT-PCR studies confirmed that miR-252-3p is well expressed in N5 but not in N6, whereas let-7-5p, miR-100-5p and miR-125-5p showed the reverse pattern. [score:3]
The involvement of let-7, miR-100 and miR-125 in metamorphosis has been demonstrated in other insects. [score:1]
4. The involvement of let-7, miR-100 and miR-125 in metamorphosis has been shown in other insects. [score:1]
Twelve of these 40 miRNAs were then investigated further by qRT-PCR and results indicated that miR-252-3p was well expressed in N5 but not in N6, whereas let-7-5p, miR-100-5p and miR-125-5p showed the reverse pattern. [score:1]
Three miRNAs emerged as important factors in the metamorphic stage (N6): let-7-5p, miR-100-5p and miR-125-5p, whereas miR-252-3p appears to be important in the pre-metamorphic stage (N5). [score:1]
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2
[+] score: 35
These data indicated that processed let-7 miRNA did not directly regulate the processing of miR-100 or miR-125. [score:3]
Sponge constructs targeting miR-100, let-7 and miR-125 were designed based on Loya et al. [84] and Bejarano et al. [85]. [score:3]
Quantitative RT-PCR analysis of miR-100, let-7 and miR-125 confirmed the absence of miRNA expression in each of the deletion lines (Fig 2A). [score:3]
This result indicated that, in addition to their role in modulating lifespan, let-7 and miR-125 promoted disease pathogenesis while miR-100 did not. [score:3]
Consistent with our previous analysis [37], young let-7-C [hyp] mutant males expressed reduced levels of miR-100 (9.2±4.6% of control), let-7 (22±10.2% of control) and miR-125 (33±7.2% of control) that decreased further as the adults aged (Fig 1B). [score:3]
Using miRNA “sponge” constructs designed to individually inhibit miR-100, let-7, or miR-125 (miR-100SP, let-7SP, or miR-125SP), we tested whether loss of any of these miRNAs’ activities enhanced the retinal degeneration in the FXTAS mo del. [score:3]
However, this analysis also revealed cross-regulatory relationships between the three miRNAs: loss of let-7 resulted in reduced levels of both miR-100 (0.29 fold relative to control) and miR-125 (0.35 fold relative to control), while loss of miR-100 and miR-125 resulted in increased levels of let-7 (2.5 fold relative to control) and miR-100 (2.5 fold relative to control), respectively (Fig 2A). [score:2]
We note that Truscott et al. also recently found evidence for cross-regulatory interaction between let-7-C miRNAs [41], although their results were slightly different— deletion of let-7 and miR-100 but not let-7 alone reduced miR-125 levels—probably due to technical differences in the constructs used. [score:2]
While the human let-7-a2 hairpin restored the let-7 mediated repression of its sensor, it did not restore miR-100 and miR-125 mediated repression (Fig 2B, construct 5). [score:1]
New transgenes were generated using previously reported P-element based transgenes containing either the full-length let-7-C locus or variants in which miR-100, let-7, and/or miR-125 were specifically deleted [33]. [score:1]
The hairpins corresponding to pri-miR-100, pri-let-7 and pri-miR-125 were deleted using splicing by overlap extension PCR. [score:1]
The luciferase reporters used in Fig 2B were psiCHECK plasmids bearing six perfect sites for either miR-100, let-7 or miR-125 downstream of a Renilla luciferase gene were used and have been described previously [38]. [score:1]
These data indicated that loss of either let-7 or miR-125 but not miR-100 caused behavioral and morphological changes that were normally seen in much older flies and were indicative of rapid aging of the brain. [score:1]
The let-7-Complex (let-7-C) locus in Drosophila encodes an evolutionarily conserved cluster of three co-transcribed miRNAs: miR-100, let-7 and miR-125, the orthologue of C. elegans lin-4 [33, 34]. [score:1]
Instead, we favor a mo del where the rate of let-7 processing has an effect on the rate of miR-100 and miR-125 processing, a mo del consistent with processing of other polycistronic microRNAs [41]. [score:1]
let-7-Complex miRNAs modulate age -associated processes in the brainThe let-7-Complex (let-7-C) locus in Drosophila encodes an evolutionarily conserved cluster of three co-transcribed miRNAs: miR-100, let-7 and miR-125, the orthologue of C. elegans lin-4 [33, 34]. [score:1]
let-7-C locus transgenesNew transgenes were generated using previously reported P-element based transgenes containing either the full-length let-7-C locus or variants in which miR-100, let-7, and/or miR-125 were specifically deleted [33]. [score:1]
In order to distinguish the roles of the three let-7-C miRNAs, we generated a set of rescuing transgenes with either miR-100, let-7 or miR-125 deleted. [score:1]
Importantly, a let-7-C transgene that substantially restored miR-100 levels (59.4±11.1% of control), let-7 levels (50.3±24% of control) and miR-125 levels (108±28%) in 3-day old adults (let-7-C [hyp] rescue in Fig 1B) rescued the lifespan and age -dependent climbing defects as well as the brain deterioration of let-7-C [hyp] mutants (Fig 1C, 1D and 1G). [score:1]
miR-125 and let-7 mutants display reduced lifespan and neurodegenerationIn order to distinguish the roles of the three let-7-C miRNAs, we generated a set of rescuing transgenes with either miR-100, let-7 or miR-125 deleted. [score:1]
Then, to test whether this effect was due to the absence of mature let-7 or some other cause (e. g. altered RNA conformation of the Δlet-7 primary transcript that reduced miR-100 and miR- 125 processing), we generated a chimeric UAS let-7-C cDNA construct in which the Drosophila let-7 hairpin was replaced with the human let-7-a2 hairpin that encoded the same mature let-7 but has a different hairpin structure. [score:1]
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3
[+] score: 20
Consistently, we propose that in flies the precursor of dme-let-7 is regulated on a level of hairpin stability because expression profiles of dme-let-7 and dme-let-7* are highly similar (r = 0.93) but each of them is slightly different from profiles of dme-mir-100 (r = 0.27 and 0.35, respectively) and dme-mir-100* (r = 0.29 and 0.53, respectively). [score:4]
The expression profiles of dme-mir-305, dme-mir-100, dme-mir-992 and dme-mir-283 are also weakly correlated to at least one miRNA from their clusters. [score:3]
In accord with our observations, the expression profiles of dme-mir-100, dme-mir-992, dme-mir-283 and dme-mir-306 have low correlation coefficients with profiles of the corresponding miRNAs*. [score:3]
It is hard to interpret unambiguously the data but it seems that independent regulation of dme-mir-100 and dme-let-7 is occurred. [score:2]
A weak correlation of dme-mir-100 and dme-mir-100* (r = 0.29) demonstrates the possibility of dme-mir-100 regulation during its maturation. [score:2]
Analysis of the pair correlation coefficients of dme-mir-100, dme-let-7 and dme-mir-125 for the 100~125 cluster demonstrates the complex nature of post-transcriptional regulation (Figure 3d). [score:2]
For dme-let-7 and dme-mir-100 the possible mechanisms of their post-transcriptional regulation are the tissue-specific uridylation and A-to-I editing of their precursors respectively (see above). [score:2]
This is confirmed by finding of the noticeable tissue-specific A-to-I editing of dme-mir-100 in male heads and Kc167 cell line [30]. [score:1]
Dme-mir-125 and dme-mir-125* correlate both to dme-mir-100 and dme-let-7, thus complicating the further analysis. [score:1]
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4
[+] score: 14
bmo-mri-2a, bmo-miR-100, bmo-miR-276b and bmo-let-7d were also expressed in larva, pupa and moth; bmo-mri-2a and bmo-miR-100 were expressed most strongly in pupa; bmo-miR-276b was expressed most strongly in moth; bmo-let-7d was expressed most weakly in moth. [score:9]
bmo-let-7b, bmo-let-7c, bmo-miR-9, bmo-miR-9*, bmo-miR-100-like, bmo-miR-263a, bmo-miR-31 and bmo-bantam were expressed in larva and pupa, but were not detected in moth; of these miRNAs, bmo-miR-9 and bmo-miR-9* are also complementary miRNAs. [score:3]
A novel small RNA was found using a probe designed to detect the known miRNA aga-miR-100. [score:1]
This novel small RNA was designated bmo-miR-100-like. [score:1]
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5
[+] score: 13
Specifically, Legeai et al. 16 reported that 17 miRNAs from A. pisum shows significant differences in their steady-state levels between two morphs, in which Let-7 and miR-100 with similar expression patterns were up-regulated and miR-2a was down-regulated between oviparae and the two other parthenogenetic morphs (virginoparae and sexuparae). [score:9]
This study analogously shows that Let-7 and miR-100 are significantly down-regulated among wingless S. avenae (Table 2, Fig. 4E), suggesting that Let-7 and miR-100 may be import in the differentiation of aphid morphotypes. [score:4]
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6
[+] score: 7
Importantly, profiling of Drosophila microRNA expression in dissected thoracic muscles, had previously demonstrated miR-124, miR-100, miR-277 and miR-304 expression in these muscles 35. [score:5]
We selected dme-miR-92a, dme-miR-100 and dme-miR-124 based on data generated in our laboratory and their orthology with human miRNAs. [score:1]
57. miRNA sponge lines (UAS-miR-SP) for dme-miR-92a, dme-miR-100, dme-miR-124, dme-miR-277, dme-miR-304 and scramble-SP (control) were obtained from Dr. [score:1]
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7
[+] score: 5
Phylogenetic analysis, target gene prediction and pathway analysis showed that, among the 13 conserved miRNAs (miR-1, miR-100, miR-10a, miR-124, miR-125, miR-184, miR-33, miR-34, miR-7, miR-9, miR-92a, miR-92b and miR-let7), several highly conserved miRNAs (miR-1, miR-7 and miR-34) targeted the same or similar genes leading to the same pathways in shrimp, fruit fly and human (Figure 3b). [score:5]
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8
[+] score: 5
Other miRNAs from this paper: dme-let-7, dme-mir-125
During fly metamorphosis, the expression of let-7 complex (let-7C), a polycistronic locus encoding the let-7, miR-100 and miR-125 miRNAs, is under direct control by the steroid hormone ecdysone. [score:4]
Deletion of the let-7 gene is sufficient to induce this phenotype, while deletion of either miR-100 or miR-125 genes is not enough to recapitulate the DIOM phenotype. [score:1]
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9
[+] score: 5
A second microRNA cluster that we observed to be duplicated in some species is the mir-100/ let-7/ mir-125 cluster (supplementary fig. S4, online). [score:1]
In P. tepidariorum we found one complete cluster (supplementary fig. S4, online), and additional copies of mir-100 and mir-125 on separate scaffolds but no paralog of let-7 (supplementary fig. S4, online). [score:1]
The arrangement of these microRNAs in S. mimosarum is similar to P. tepidariorum (supplementary fig. S4, online) although the two mir-100 paralogs are on separate scaffolds (supplementary fig. S4, online). [score:1]
Acanthoscurria geniculata has a complete mir-100/ let-7/ mir-125 cluster and two additional copies of mir-125 are found on separate scaffolds (supplementary fig. S4, online). [score:1]
Therefore the mir-71/ mir-2 and mir-100/ let-7/ mir-125 clusters appear to have been duplicated in spiders and scorpions (Arachnopulmonata) and L. polyphemus but not other chelicerates or mandibulates that we surveyed. [score:1]
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10
[+] score: 5
Indeed, mir-100 is the evolutionarily most ancient microRNA, and it is conserved in metazoans and cnidarians (45, 46). [score:1]
Collectively, 14 clusters (the majority of our data set) emerged within the insects (Figure 3), that is, the Melanogaster, Drosophila and insect lineages in Table 1. Two clusters are conserved among all metazoans: the mir-125/let-7/mir-100 and the mir-92a/mir-92b clusters. [score:1]
The other cluster, mir-125/let-7/mir-100, is probably the only conserved cluster in most metazoans. [score:1]
Only two clusters are conserved in all metazoans, mir-92a/mir-92b and mir-125/let-7/mir-100. [score:1]
A notable case is the mir-125/let-7/mir-100 cluster, which is highly conserved across the animal kingdom, although in both Nematodes (56) and in Platyhelminthes (57), mir-125 and let-7 are not clustered, and mir-100 is lost. [score:1]
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11
[+] score: 5
Surprisingly, we also could not identify the S. maritima homologue of mir-125, a member of the ancient mir-100/let-7/mir-125 cluster, which is found in almost all bilaterians and has a well-established function in the regulation of development of many species [127]– [129]. [score:3]
Mir-100 and let-7 are well-conserved and localized within a 1 kb region on the same scaffold in S. maritima. [score:1]
Whilst we cannot rule out the possibility that the missing mir-125 is an artefact of the draft-quality genome assembly, the size of the scaffold strongly suggests that it is not present in the mir-100/let-7 cluster. [score:1]
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12
[+] score: 3
Among the highly expressed; bantam, miR-184, miR-81, miR-100, miR-92, miR-2766, miR-279 are listed in the top (S1 Table) and these findings are in concurrence with recent reports [19]. [score:3]
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13
[+] score: 3
In our set of C. elegans_human ≥70% homologs, the following do not have ≥7 nucleotides of continuous similarity at the 5′ end: cel-miR-57 with hsa-miR-99a and hsa-miR-100; cel-miR-236 with hsa-miR-141 and hsa-miR-200a; and cel-miR-266 with hsa-miR-25*, hsa-miR-301a and hsa-miR-301b. [score:1]
8 of the 40 Drosophila miRNAs with ≥70% homologous sequences in humans show extensive overall similarity with 5′ mismatches: dme-miR-8 with hsa-miR-141 and hsa-miR-200a, dme-miR-10 with hsa-miR-100 and hsa-miR-99a, dme-miR-100 with hsa-miR-10a and hsa-miR-10b, dme-miR-125 with hsa-miR-10a and hsa-miR-10b, dme-miR263a with hsa-miR-183, dme-miR-263b with hsa-miR-183, dme-miR-306 with hsa-miR-873, and dme-miR-993 with hsa-miR-100*. [score:1]
Most of the fly ≥70% full length homologs exhibit blocks of ≥7 nt identity at the 5′ end except the following 10: dme-miR-10, dme-miR-100, dme-miR-263a, dme-miR-263b, dme-miR-954, dme-miR-966, dme-miR-1009, dme-miR-1010, dme-miR-iab-4-3p and dme-miR-iab4as-3p. [score:1]
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14
[+] score: 3
However, differential signal for three of the tiling array positive miRNA candidates suggests that those miRNAs (C6617, C4222 and ame-mir-100) may have roles in bee brain or thorax. [score:1]
Among the latter six, C4222, C6617 and ame-mir-100 exhibited differential signal strength in the two tiling array experiments. [score:1]
Other examples of clustered miRNAs or multicopy miRNAs include: novel miRNA C5152a antisense to C5152b; novel C5303 overlapping ame-mir-137; ame-mir-9b overlapping the ame-mir-79 locus, but on the opposite strand; ame-mir-12 near ame-mir-283; ame-mir-275 near ame-mir-305; ame-mir-277 near ame-mir-317 and ame-mir-34; C1504 near ame-mir-375; and ame-let-7 on the same scaffold as ame-mir-100. [score:1]
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15
[+] score: 3
They exert the opposite effects on the expression of temporal miRNA genes, such as mir-34, mir-100, mir-125 and let-7 [22]. [score:3]
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16
[+] score: 3
For example, expression of let-7, miR-100 and miR-125, which cluster at the same genomic locus, is markedly induced upon 20-HE treatment [63]. [score:3]
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17
[+] score: 2
In fact, many of these miRNAs, such as the let-7 family, mir-29 family, mir-26 family, mir-140-3p, mir-30d, mir-7, mir-191, mir-21, mir-100, mir-15a, and mir-16, play an important role in development and differentiation [36- 41]. [score:2]
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18
[+] score: 1
In this last subgroup, the three miRNAs with homology to the D. melanogaster polycistronic locus let-7-Complex (let-7-C) (i. e. mir-100, mir-125, and let-7) were present. [score:1]
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