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193 publications mentioning hsa-mir-302a (showing top 100)

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-302a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 303
Other miRNAs from this paper: hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-302e, hsa-mir-302f
Overexpression of miR-302 did not lead OCT4 negative hMSCs to express OCT4 and acquire the teratoma formation, which suggested that the regulation of miR-302 on pluripotency and teratoma formation by direct suppression of AKT1 may be dependent on the high endogenous expression of OCT4 in cells. [score:11]
Then miR-302 -overexpressing lentiviral vector (lenti-miR-302s) that expresses a series of connected miR-302a/b/c/d sequences was used to upregulate the expression of miR-302s in hMSCs (Supplementary Figures S3A and B). [score:10]
In addition, the upregulation of miR-302 in AKT1 -overexpressed hNT-2 cells could inhibit AKT1 and rescue the expression of OCT4. [score:10]
[20] We found that miR-302 was gradually downregulated during EB differentiation (Figure 6a), while at the same time, AKT1 was gradually upregulated, OCT4 and SOX2 were downregulated (Figure 6b). [score:10]
21, 22, 23, 33, 34, 35 Consistent with these results, we revealed that the expression levels of CCND1, CDK4, CDK6, CDKN1A and TGF β were increased in miR-302 downregulated-hNT-2 cells and decreased in miR-302 upregulated-hMSCs (Supplementary Figures S4B and C). [score:9]
showed that OCT4 was decreased in AKT1 -overexpressed hNT-2 cells, while its expression was increased in AKT1 suppressed-hNT-2 cells and was accompanied with morphological changes similar to the effect of miR-302 on the expression of AKT1 and OCT4 (Figures 6c and e). [score:9]
displayed that the overexpression of miR-302 significantly suppressed >58% of the reporter luciferase activity of the AKT1-WT reporter plasmid but not that of the AKT1-MUT reporter plasmid, suggesting a direct targeting relationship between miR-302 and the 3'UTR of AKT1 (Figure 5b). [score:8]
Upregulation of miR-302 in AKT1 -overexpressed hPSCs could inhibit AKT1 and rescue the protein level of OCT4. [score:8]
These results strongly confirmed that miR-302 can directly target AKT1 and suppress its expression. [score:8]
Overexpression of miR-302 also significantly decreased AKT1 expression in hMSCs (Supplementary Figure S5A), whereas OCT4 always maintained at undetectable level regardless of the expression changes of miR-302 and AKT1 in hMSCs (Supplementary Figures S6A and B). [score:7]
[51] We found a marked suppression of cell cycle progression in miR-302 -suppressed-hNT-2 cells, while an increase in the number of S phase cells in miR-302 -overexpressed hMSCs. [score:7]
miR-302 can directly target and regulate the expression of AKT1 in pluripotent and adult stem cells. [score:7]
Here, we observed that in addition to directly targeting cell cycle -associated genes, miR-302 also affects the expression of total AKT1 and phospho-AKT mainly at S473 locus. [score:6]
In addition, the expression of OCT4 was markedly reduced in teratoma generated from miR-302 -downregulated hNT-2 cells (Figure 7a). [score:6]
Furthermore, miR-302 upregulation could not significantly change the expression level of OCT4 in siAKT1 -transfected hNT-2 cells (Figures 6c and d), while repression of miR-302 led to the opposite effects. [score:6]
We found that inhibition of miR-302 causes a significant decrease in self-renewal ability and promotes differentiation accompanied by downregulation of OCT4, suggesting that high level of endogenous miR-302 may be responsible for the maintenance of self-renewal and pluripotency in hPSCs. [score:6]
The positive correlation between miR-302 and OCT4, and negative correlation between miR-302 and AKT1 suggested that miR-302 may enhance the expression of pluripotency regulators through suppressing AKT1. [score:6]
TargetScan, PicTar and Miranda suggested that many genes including both positive and negative G1 to S transition -associated regulators are candidate targets of miR-302 (Figure 3f). [score:6]
High expression of endogenous miR-302 in hPSCs is a contributing factor for the pluripotency and teratoma formation through maintaining OCT4 at high level by directly inhibiting AKT1. [score:6]
Downregulation of miR-302 inhibits the teratoma formation of hPSCs. [score:6]
Previous evidence has demonstrated that the cell cycle promoters CCND1 and CDK2, and the inhibitors CDKN1A, RB1, RBL1, RBL2, LATS2 and TGFBR2, are direct targets of miR-302. [score:6]
5, 32 To explore the intrinsic mechanisms underlying the regulation of teratoma formation by miR-302, we analyzed the cell cycle distribution and the expression patterns of key cell cycle regulators associated with the G1 to S transition in hESCs, hNT-2 cells and hMSCs. [score:5]
These results revealed that miR-302 indirectly regulates OCT4 protein level by suppressing AKT1 and subsequently avoiding OCT4's degradation. [score:5]
We confirmed that AKT1 is a direct target of miR-302 in the regulation of pluripotency and differentiation of hPSCs. [score:5]
miR-302 contributes to the pluripotency and teratoma formation of hPSCs by maintaining OCT4 expression via suppressing AKT1. [score:5]
Importantly, in highly differentiated teratoma of patients, which are more common clinically, the expression of miR-302 is very low or even undetectable, and the expression of OCT4 is still undetectable. [score:5]
Therefore, our findings revealed that high expression levels of endogenous miR-302 in hPSCs are beneficial for the pluripotency and tumorigenicity of hPSCs by maintaining OCT4 at high level through suppressing AKT1 (Figure 7d). [score:5]
5, 7, 8, 9, 33, 35 To date, both positive and negative cell cycle -associated factors have been reported as direct targets of miR-302. [score:4]
[52] Thus, our data suggest that the negative cell cycle regulators are dominant targets of miR-302 in ESCs, hNT-2 cells and hMSCs. [score:4]
qRT-PCR and western blot analyses showed that miR-302 can regulate the expression of total AKT1 and phosphorylated AKT1 mainly at S473 locus (Figures 5c and d; Supplementary Figures S4B and C) both in hNT-2 and hMSCs. [score:4]
However, upregulation of miR-302 cannot lead OCT4 negative hMSCs to acquire the teratoma formation. [score:4]
38, 39 We found that miR-302 could regulate the expression of AKT1 (Supplementary Figures S4B and C). [score:4]
miR-302 regulates pluripotency by promoting self-renewal and suppressing differentiation. [score:4]
[18] We found that OCT4 was markedly reduced in teratoma generated from miR-302 -downregulated hNT-2 cells, which is in agreement with our results in vitro. [score:4]
miR-302 dominantly regulates a set of cell cycle inhibitors and promotes the G1 to S transition. [score:4]
Our findings suggested that miR-302 can enhance proliferation through the dominant regulation of a set of cell cycle inhibitors, and result in rapid G1 to S transition. [score:4]
23, 48, 49 We revealed that downregulation of miR-302 significantly decreased the rate of tumor formation and reduced the tumor volume in hPSCs. [score:4]
To further validate whether miR-302 can directly target AKT1, we designed dual luciferase reporter gene vectors containing either wild type (AKT1-WT) or mutated (AKT1-MUT) putative 3′UTR sequences for miR-302 -binding (family ‘seed sequence') and inserted them into the 3′UTR of the luciferase genes. [score:4]
Thus, we speculated that AKT1 might be one of the direct targets of miR-302 that controls the self-renewal and pluripotency of hPSCs. [score:4]
To clarify whether miR-302 mediates pluripotency and differentiation of hPSCs through the regulation of AKT1, we first analyzed the expression of miR-302 during embryoid body (EB) formation. [score:4]
miR-302 can promote the proliferation and self-renewal both in hPSCs and hMSCs through dominantly regulating a set of cell cycle inhibitors and accelerating the G1 to S transition. [score:4]
We presumed that high endogenous expression of miR-302 in hPSCs might be responsible for their teratoma formation. [score:3]
In summary, our findings first uncover that miR-302 indirectly regulates OCT4 by suppressing AKT1, which provides hPSCs two characteristics related to their potential for clinical applications: the benefit of pluripotency and the hindrance of teratoma formation. [score:3]
Our miRNA microarray and TaqMan qRT-PCR data revealed that the endogenous expression levels of miR-302 family were high in hESCs and hNT-2 cells but very low in hMSCs (Figures 1a and b; Supplementary Figures S1E and F and Supplementary Table S1). [score:3]
We speculated that miR-302 might be involved in maintaining the low expression levels of these genes in hESCs. [score:3]
Thus, we next analyzed the impact of miR-302 on the proliferation of hPSCs and found that cell growth was suppressed gradually with an increase in the concentration of miR-302s antagomir (Figure 2a). [score:3]
The ectopic expression of miR-302 is able to reprogram and promote human somatic cells to ESC-like cells. [score:3]
hPSCs have strong teratoma formation ability and high endogenous expression of miR-302. [score:3]
After the cells were infected with the miR-302 overexpressing GFP-labeled lentiviral vector (lenti-miR-302s), GFP -positive cells were sortinged by FACSCalibur (BD). [score:3]
These results strongly demonstrated that miR-302 can maintain the expression of OCT4 at high level, at least in part by repressing AKT1. [score:3]
These results showed that the inhibition of miR-302 negatively impacted self-renewal and pluripotency and promoted the differentiation of hPSCs. [score:3]
shRNA was used to suppress endogenous expression of miR-302s and to investigate the function of miR-302 on pluripotency and differentiation of hPSCs. [score:3]
More recently, miR-302 has been reported to be capable of regulating Brg1 chromatin remo deling complex composition in hESCs, and subsequently regulating pluripotency by positively influencing mesendodermal differentiation. [score:3]
Moreover, the expression of miR-302 is very low or almost undetectable in highly differentiated patient-derived teratoma as compared with hNT-2 cells generated malignant teratoma xenografts (Figure 7b). [score:2]
Whether upregulation of miR-302 can drive hMSCs to acquire a higher differentiation potential is worthy of deep investigation. [score:2]
24, 41, 42, 43, 44 However, there is a big controversy about what role miR-302 has in the regulation of stemness, cell proliferation, tumorigenesis and differentiation. [score:2]
Taking into account the positive feedback regulation involved in the G1 to S transition, self-renewal and pluripotency in hPSCs, 5, 36, 37 we further assessed the role of miR-302 in self-renewal and pluripotency. [score:2]
53, 54 Recently, the role of miR-302 in nerve development has been reported. [score:2]
[40] Here, we demonstrate that miR-302 has an important role in regulating cell proliferation, self-renewal, pluripotency, differentiation and teratoma formation. [score:2]
miR-302 promotes the proliferation of pluripotent and adult stem cells. [score:1]
These results demonstrated that miR-302 can promote cell proliferation both in pluripotent and adult stem cells. [score:1]
In all, 5 × 10 [4] 293 T cells per well in 24-well plates were co -transfected with 1  μg pRL-TK vector with or without the synthetic fragment of the AKT1 3′UTR and 0.1  μg pGL-3 control vector with firefly luciferase reporter gene, and 100 nM miR-302 mimics or miR-NC mixed with Lipofectamine 2000 (Invitrogen), respectively, according to the manufacturer's instructions. [score:1]
These results indicated that miR-302 promotes cell growth and proliferation partially through promoting the G1 to S transition. [score:1]
Cells were transfected with 200 nM of miR-302s antagomir (50 nmol each of miR-302a antagomir, miR-302b antagomir, miR-302c antagomir and miR-302d antagomir), 200 nM miR-302s mimics (50 nmol each of miR-302a, miR-302b, miR-302c and miR-302d) or 200 nM negative control NC or 200 nM negative control antagomir as a control (Genepharma, Shanghai, China) (see Supplementary Table S5 in the ). [score:1]
This result coincides with recent report that miR-302 induces proliferation in human adipose tissue-derived MSCs. [score:1]
miR-302s antagomir (miR-302a, miR-302b, miR-302c and miR-302d in combination) was used to silence the endogenous miR-302s in vitro and in vivo (Supplementary Figure S2A). [score:1]
[57] These findings suggested a complicated relationship between pluripotency and differentiation related to miR-302. [score:1]
Thus, miR-302 is able to promote the teratoma formation of hPSCs in vitro and in vivo. [score:1]
Bioinformatics analysis showed that there is a 7-bp sequence within the 3'UTR of AKT1 that is complementary to the sequence of miR-302 (Figure 5a). [score:1]
[8] Therefore, to further analyze the effects of miR-302 on the modulation of differentiation, hESCs were subjected to EB formation and then placed back into ESC conditions. [score:1]
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[+] score: 168
miR-302 members also can directly inhibit the expression of Cyclin D1/2, CDK2 and ARID4a (AT-rich interacting domain 4a, also known as RBP1), which represses the phosphorylation of pRB, leading to failed expression of cell cycle genes and cellular G1 phase arrest (figure 2 b) [57, 58]. [score:8]
miR-302 members strongly suppress BMI-1 to stimulate CDKN2A (cyclin -dependent kinase inhibitor 2A, or p16) expression, thus giving rise to decrease in the output of CDK4/6 and Cyclin D complex and finally repressing the G1-to-S transition. [score:7]
CDKN1A (cyclin -dependent kinase inhibitor 1, also known as p21), which was referred to as an inhibitor to Cyclin E/CDK2 complex, was the first verified target of the miR-302/367 cluster [55, 56]. [score:7]
Overexpression of the miR-302/367 cluster significantly increased the conversion of reprogrammed iPS cells by repressing MBD2 expression, thereby augmenting Nanog expression [65]. [score:7]
The miR-302/367 cluster is wi dely distributed in vertebrates and plays vital roles in cellular self-renewal, differentiation and reprogramming, mainly through targeting the key genes in cell cycle regulation, cellular signalling regulation and epigenetic regulation. [score:6]
To begin with, the miR-302/367 cluster was found to be involved in regulating cell signalling pathways, including TGF- β/Nodal signalling, PI3K–AKT signalling and BMP signalling, by directly repressing expression of some key components in the pathways [24, 37, 47]. [score:5]
miR-302 members can target different epigenetic factors resulting in global demethylation in target cells [61]. [score:5]
The miR-302/367 cluster, specifically expressed in embryonic stem cells, induced pluripotent stem cells (iPSs) or tumour cells, represses the targets mentioned above, to coordinate proliferation, differentiation, pluripotency maintenance and reprogramming. [score:5]
4.2. miR-302 members can target different epigenetic factors resulting in global demethylation in target cells [61]. [score:5]
Interestingly, the 3′UTRs of upregulated transcripts in Dicer -null ESCs were shown to be enriched for the ‘GCACUUU’ and ‘AGCACUU’ motifs, complementary to the miR-302 family seed sequence, indicating the importance of the miR-302 family in the regulatory network in ESCs [24, 25]. [score:5]
The miR-302/367 cluster regulates the pathways through silencing key components, and the feedback of the regulated pathways can impact the expression of the cluster. [score:5]
Most recent publications on miR-302 members have supported a positive correlation between miR-302/367 upregulation and BMP signalling promotion. [score:4]
The remaining verified targets of miR-302/367 are key mediators of a diverse range of processes, including epigenetic regulation and glucose metabolism. [score:4]
In addition, miR-302 members could negatively regulate the level of Lefty1 and Lefty2 (Nodal inhibitors), and thus became upstream modulators of the TGF- β/Nodal signalling pathway, striking a balance between pluripotency and differentiation [70]. [score:4]
Using similar experimental approaches, Gata6 was identified as a new transcriptional regulator to activate the expression of miR-302/367 cluster in mouse embryos [39, 40]. [score:4]
In addition, miR-302 members can mediate other pathways to regulate the cell cycle via targeting the transcripts of epidermal growth factor receptor (EGFR), C–C chemokine receptor type 5 (CCR5), C–C motif ligand (CCL5) and C–X–C chemokine receptor type 4 (CXCR4) [58– 60]. [score:4]
M112.390898) 43 Brautigam C, Raggioli A, Winter J 2013 The Wnt/beta-catenin pathway regulates the expression of the miR-302 cluster in mouse ESCs and P19 cells. [score:4]
miR-302 members directly repressed the expression of the transforming growth factor beta receptor 2 (TGFBR2), and Ras homologue gene family, member C (RHOC) genes, resulting in facilitation of EMT [68, 69]. [score:4]
miR-302 members directly silenced the expression of CDKN1A and hence augmented the abundance of Cyclin E/CDK complex, promoting the transition of mouse embryonic stem cells from G1 to S phase [56]. [score:4]
M111.308528) 42 Kang H, Louie J, Weisman A, Sheu-Gruttadauria J, Davis-Dusenbery BN, Lagna G, Hata A 2012 Inhibition of microRNA-302 (miR-302) by bone morphogenetic protein 4 (BMP4) facilitates the BMP signaling pathway. [score:3]
miR-367 was slightly different in the seed sequence from miR-302a–302d, but they shared a portion of common mRNA targets. [score:3]
However, a subsequent publication revealed that miR-302 members could inhibit human pluripotent stem cell proliferation by enhancing multiple G1 phase arrest pathways [57]. [score:3]
Furthermore, CHIP assays revealed a physical interaction between Sox2 and the binding sites [38], verifying that Sox2 is the upstream regulator of the miR-302/367 expression. [score:3]
Three BMP inhibitors, TOB2, DAZAP2 and SLAIN1, were silenced via binding of mature miR-302 members to the 3′ UTRs of their transcripts, leading to repression of stem cell differentiation and maintenance of stem cell pluripotency [66]. [score:3]
Henceforward, numerous targets of miR-302/367 cluster have been identified. [score:3]
A recent study has also revealed that an anti-allergy drug, tranilast, promoted miR-302 members expression through binding to the two aryl hydrocarbon receptor binding motifs in the promoter [44]. [score:3]
In addition, some regulators could indirectly affect the transcriptional level of the miR-302/367 cluster via a diverse range of signalling pathways. [score:3]
Figure 2. (a) The transcriptional factors and main targets of the miR-302/367 cluster. [score:3]
Increasing evidence has directly demonstrated that the members of the miR-302/367 cluster play a critical role in regulation of the balance of G1-to-S transition. [score:3]
Cyclin D1 and CDK4 were the first miR-302/367 targets identified, by means of reporter assays [38, 46]. [score:2]
Accumulating evidence demonstrates that the miR-302/367 cluster plays significant roles in regulation of cellular proliferation, differentiation and reprogramming. [score:2]
For some well-annotated genomes, mir-302 sequences were directly extracted from the database. [score:2]
The miR-302/367 cluster has been demonstrated to be involved in regulation of various cellular signalling pathways, such as the BMP signalling pathway and TGF- β/Nodal/Smad-2/3 pathway, to coordinate different biological processes. [score:2]
Indeed, miR-302 members repressed lysine-specific histone demethylase 1 and 2 (AOF1 and AOF2) and methyl-CpG binding proteins (MECP1 and MECP2), leading to destabilization of DNA methyltransferase 1 which is involved in genome-wide demethylation and consequently promotes reprogramming and iPS cells development [64]. [score:2]
The RNA sequencing data from H. sapiens and Mus musculus confirmed that the 3′ arm is highly expressed and more conserved compared with the 5′ arm in most miR-302 family members (figure 1 c) [28– 30]. [score:2]
Of the 118 TFs, seven pertained to the miR-302/367 cluster, including the previously known Oct 3/4 and 6 novel TFs (figure 2 a). [score:1]
However, according to multiple sequence alignment, we found that the mir-302 of P. marinus (pma-mir-302) was not contained in the mir-302/367 family. [score:1]
The miR-302/367 cluster gene was found to be located in an intron on the 4q25 region of human chromosome 4, and transcribed by RNA polymerase II (Pol-II) to generate a capped and polyadenylated miRNA precursor that possessed eight miRNAs: miR-367, 302d, 302c-5p, 302c-3p, 302a-5p, 302a-3p, 302b-5p and 302b-3p [23]. [score:1]
The miR-302/367 cluster, composed of several intronic miRNAs, was initially proposed to be transcribed with their host gene LARP7. [score:1]
The starting point of comparative genomic analysis of the mir-302/367 cluster was the retrieval of mir-302 members and mir-367 precursor sequences. [score:1]
Increasing studies in the future will provide us with a clear mechanism by which miR-302 members fulfil the cellular self-renewal, differentiation and reprogramming in a more integrated network. [score:1]
The whole genome of the amphibian X. tropicalis codes two miRNAs, miR-302 and miR-367, located in the intron of the LARP7 gene. [score:1]
Red arrows depict miR-302 family members, green arrows depict miR-367, black arrow depicts pma-miR-302 and blue arrow depicts bird-specific miR-1811 sequence. [score:1]
The role of the miR-302/367 cluster is expanding. [score:1]
It was also observed that the seed sequence of Gallus gallus miR-1811, a bird-specific miRNA located in the miR-302/367 cluster gene locus, shows a high similarity to miR-367, indicating that miR-1811 might have been generated by tandem duplication of miR-367. [score:1]
pma-mir-302 only shared the seed sequence of the 3′ mature miRNAs with the 3′ mature sequence of the mir-302/367 family (figure 1 b). [score:1]
mir-302 precursor sequences: miRBase accessions MI0000738, MI0000772, MI0000773, MI0000774, MI0001211, MI0003700, MI0003701, MI0003702, MI0004878, MI0017123, MI0006903, MI0006904; Ensemble Genome Browser accessions ENSDNOG00000038602, ENSDNOG00000043944, ENSDNOG00000027052, ENSDNOG00000026812, ENSDNOG00000017050, ENSDNOG00000016990. [score:1]
After identification of the gene structure of the miR-302/367 cluster by means of 5′ RACE assay, 3′ RACE assay and sequence alignment, several transcriptional factors (TFs), Oct3/4, Cdx2, Sox2, Rex1 and Nanog, were predicted to be capable of targeting the promoter of the cluster using bioinformatic methods [23]. [score:1]
The miR-302/367 cluster is highly conserved and vertebrate-specific. [score:1]
Whereas most mature miRNAs from the miR-302 family generally originate from the 3′ arm of the hairpin precursor, a small quantity of mature miR-302 members are derived from the opposite arm. [score:1]
More specifically, miR-302 members can fine-tune stem cell self-renewal through promotion of BMP signalling. [score:1]
A combined searching strategy was undertaken to identify mir-302/367 family members in multiple genomes from Ensemble Genome Database (http://www. [score:1]
Another study found that the miR-302/TGF- β/Nodal/Smad-2/3 pathway was also involved in epithelial–mesenchymal transition (EMT). [score:1]
It was experimentally demonstrated that Oct3/4, Nanog, Rex1 and Sox2 act as transcriptional activators of the miR-302/367 cluster [37, 38]. [score:1]
Biological functions of the miR-302/367 cluster. [score:1]
Higher vertebrates such as mammals, birds and reptiles commonly possess ‘classical’ structure, including four miR-302 members and one miR-367, in the cluster locus. [score:1]
The results revealed that the miR-302/367 cluster is conserved among vertebrates, but the copy number and genomic location of the cluster gene vary. [score:1]
The expansion or shrinking of the miR-302 family by tandem duplication or deletion generated miR-302/367 clusters of different lengths in different species. [score:1]
TFs in the right upper corner were newly identified by the ENCODE project and the relationship of most of them to the miR-302/367 cluster is putative. [score:1]
No member of the miR-302/367 cluster has been discovered in bony fish such as D. rerio, most probably owing to the loss of this cluster during the evolutionary process. [score:1]
The fact that no homologue can be found in jawless fish and bony fish suggested the emergence of mir-302/367 at the branch leading to the tetrapoda. [score:1]
Interestingly in primates, besides the four members of miR-302, there also exist another two miR-302 members, namely miR-302e and miR-302f, which are both intergenic miRNAs and located in chromosome 11 and 18, respectively. [score:1]
As shown in figure 1 a, the ancient vertebrate P. marinus contains only one copy of miR-302 members. [score:1]
Figure 1. (a) Phylogenetic tree of vertebrate species and genomic organization of miR-302 and miR-367 sequences. [score:1]
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3
[+] score: 156
In addition to the inhibition of Nodal antagonists, miR-302 could promote BMP signaling by targeting the inhibitors DAZAP2, SLAIN1, and TOB2 [61]. [score:7]
For instance, methyl-DNA binding domain protein 2 (MBD2), an epigenetic suppressor of Nanog, is a direct target of miR-302 [140]. [score:6]
c-Myc downregulates TGFβ1 and TGFβ receptor 2 (TGFBR2), which is also a target of the miR-302 and miR-93 families [119, 121, 123, 133]. [score:6]
Similarly, during reprogramming, miR-302 may also indirectly promote the activation of endogenous core pluripotency genes by targeting their inhibitors. [score:6]
By inhibiting inhibitors of both branches, miR-302 plays a central role in negatively regulating neural induction in pluripotent stem cells (Figure 2). [score:6]
Conversely, Myc-activated genes and c-Myc itself are enriched among transcript that are upregulated in presence of miR-302 family members, suggesting that the pro-pluripotency activity of these miRNAs may be mediated, at least in part, by the indirect increase of Myc [110]. [score:5]
In a feedback loop, miR-302 targets the type II BMP receptor (BMPRII) in PASMCs, thus inhibiting BMP signaling. [score:5]
Upon transfection of let-7 family miRNAs, expression of Oct4, Sox2 and Nanog is inhibited, suggesting an anti-pluripotency activity for let-7. However, if miR-302 family miRNAs are co -transfected, they impair this activity and restore the levels of pluripotency markers [110]. [score:5]
We have previously demonstrated that miR-302 targets NR2F2, which in turn is a transcriptional inhibitor of Oct4 [94]. [score:5]
The specific expression of miR-302 family miRNAs is ensured by their regulation, at the transcriptional level, by the core ESC transcriptional regulatory circuitry [8, 9, 62, 63]. [score:5]
Other pluripotency factors that are directly inhibited by let-7, and indirectly activated by miR-302 family, are Lin28 and Sall4, suggesting that these miRNAs exert their function via multiple pathways [110]. [score:5]
According to the miRNA expression atlas [59], miR-302 family members are specifically expressed in embryonic cells in both mouse and human. [score:5]
We have shown that, by directly inhibiting Lefty, miR-302 is necessary for proper mesoderm and endoderm specification [87]. [score:4]
Another gene involved in EMT, RHOC, is a direct target of miR-302 [123]. [score:4]
The regulation of Nodal signaling by miR-302 seems evolutionary conserved, as the Xenopus and Zebrafish hortologues target Lefty during early embryogenesis [87, 88]. [score:4]
It has recently been shown that BMP signaling down-regulates the miR-302/367 cluster in human primary pulmonary artery smooth muscle cells (PASMCs), mouse mesenchymal cells and embryonic carcinoma p19 cells [91]. [score:4]
Downregulation of MDB2 by miR-302 is necessary to achieve a fully reprogrammed iPSC state. [score:4]
Recently, it has been shown that NR2F2 knockdown enhances reprogramming efficiency, thus mimicking miR-302 overexpression [141]. [score:4]
For instance, it has been proposed that during reprogramming miR-302 regulates multiple genes involved in cell cycle regulation, epigenetic regulation, vesicular transport, cell signaling and mesenchymal-to-epithelial transition [123]. [score:4]
We have shown that both Oct4 (at the transcriptional level) and miR-302 (post-transcriptionally) repress a common target, NR2F2 (also known as COUP-TFII) [94]. [score:3]
Therefore, miR-302 and the two transcription factors, NR2F2 and Oct4, form a feedback regulatory circuitry that regulates hESC exit from pluripotency and neural fate specification (Figure 2). [score:3]
mESCs express high levels of miR-290-295 that decline after conversion to EpiSCs and are replaced by an increase of miR-302/367. [score:3]
Similarly, undifferentiated human ESCs are dominated by the mir-302 cluster, which accounts for more than 60% of all expressed miRNAs [61]. [score:3]
miR-302 overexpression sustains pluripotency markers in differentiating hESCs [87, 139]. [score:3]
The miR-302 family targets p21 and promotes G1/S transition [79, 80]. [score:3]
As mentioned before, introduction of miR-302 family members in DGCR8 −/− mESCs increases expression of endogenous c-Myc and N-Myc downstream genes [110]. [score:3]
Further studies are necessary to address whether this reciprocal inhibition between BMP signaling and miR-302 also exist in ESCs. [score:3]
ESCs and iPSCs express a similar signature group of miRNAs, including the miR-302 family, with small differences between the two cell types [114– 116]. [score:3]
The miR-302 family miRNAs are abundantly expressed in undifferentiated ESCs and decline upon differentiation [54– 58]. [score:3]
miR-302 is deeply integrated in the core transcriptional regulatory circuitry of ESCs. [score:2]
In human ESCs, which correspond to a primed state of pluripotency, the levels of miR-302/367 are much higher than the levels of miR-371-373 and the switch to an earlier developmental state led to an increase of the levels of miR-371-373 [65– 67]. [score:2]
The promoter of the miR-290-295 cluster is directly bound and activated by c-Myc and N-Myc [7], and the miR-302 cluster is also induced by Myc [112], establishing positive feedback loops. [score:2]
Therefore, according to the mo del depicted in Figure 5, the miR-302 and let-7 miRNA families play opposite, crucial roles in regulating ESC pluripotency and differentiation. [score:2]
The miR-302 family has been involved in the TGF-β/BMP signaling pathway, which regulates embryonic stem cells pluripotency and differentiation. [score:2]
In contrast to the finding that combinations of multiple miRNA families are necessary for reprogramming [123, 127, 128], Lin et al. have reported that miR-302 alone could convert skin cancer and hair follicle cells into iPSCs in the absence of other miRNAs or RFs [129, 130]. [score:1]
Moreover, miR-302 family members are required for efficient reprogramming of somatic cells and, in combination with other miRNAs, are sufficient for iPSC generation in the absence of canonical reprogramming factors. [score:1]
This defect could be partially rescued by miR-302 family members and the unrelated miR-195. [score:1]
For simplicity, in this review we will refer to miRNAs containing the AAGUGC seed as miR-302 family. [score:1]
For both let-7 and the miR-302 families feedback loops with Myc are in play. [score:1]
Both were required for reprogramming, as miR-302 alone is not able to give rise to iPSCs in the absence of miR-367. [score:1]
Moreover, at least other two components of the cell cycle machinery, Cyclins D1 and D2, are under the control of miR-302 family members in hESCs [63, 82]. [score:1]
As mentioned above, the miR-302 host gene is under the control of Oct4, Nanog and Sox2, which ensure high miRNA levels in undifferentiated ESCs [9, 62, 63]. [score:1]
Accordingly, miR-302 family members could rescue the proliferation defects of DGCR8 mutant mESCs [73]. [score:1]
For instance, it has been shown that the combination of miR-302, miR-200c and miR-369 can reprogram both human and mouse somatic cells [128]. [score:1]
The promoters of the miR-302 and the miR-290-295 clusters are bound by Oct4, Nanog, Sox2 and Tcf3, that also promote transcription of other unrelated miRNAs. [score:1]
However, it is unlikely that the pro-reprogramming activity of the miR-302 is merely mediated by downstream activation of c-Myc. [score:1]
For instance, miR-93 and 106b (that belong to the same family and share 5/6 of the miR-302 seed) and the unrelated miR-138 enhance iPSC generation [118, 122]. [score:1]
Moreover, miR-367, but not miR-302, can be substituted by other miRNAs in alternative reprogramming cocktails. [score:1]
Interestingly, when used in combination with reprogramming factors to enhance reprogramming, miR-302 alone was almost as effective as the intact miR-302-367 cluster, whereas miR-367 alone had no effect [121]. [score:1]
The conserved miR-302/367 cluster comprises four AAGUGC seed-containing miRNAs (miR-302a, miR-302b, miR-302c and miR-302d) and the unrelated miR-367. [score:1]
Similar to the mouse system, reprogramming of human fibroblasts was also enhanced when miR-302 family members are provided along with the reprogramming factors [123]. [score:1]
This sequence is complementary to the AAGUGC seed of miR-302 family miRNAs, thus confirming their prominent role in pluripotent stem cells. [score:1]
Interestingly, in presence of the three factors plus c-Myc the reprogramming enhancement by the miR-302 family members was strongly reduced [120, 121]. [score:1]
The miR-302-367 cluster contains four miR-302 members, with the AAGUGC seed, and the unrelated miR-367. [score:1]
Much work concerned the miRNAs belonging to the miR-302 family. [score:1]
Interestingly, the conversion from a naïve to a primed state in mESCs correlates with a switch between the miR-290-295 and the miR-302/367 clusters in terms of miRNA abundance [64] (Figure 1b). [score:1]
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4
[+] score: 146
Reprogramming via miR-302/367 might involve different pathways such as targeting several epigenetic factors that result in global demethylation of the genome, inhibition of Oct4 suppressor factors, and enhancement of pluripotency markers [28, 30– 33]. [score:7]
0127878.g001 Fig 1 Transduction and continuous expression of vectors were confirmed by injecting a GFP expressing empty lentiviral vector and lentiviral particle that included the miR-302/367+GFP cluster. [score:5]
Transduction and continuous expression of vectors were confirmed by injecting a GFP expressing empty lentiviral vector and lentiviral particle that included the miR-302/367+GFP cluster. [score:5]
miR-302/367 expressing lentiviral particles were injected into the mice striata to induce transdifferentiation of targeted cells into neuroblasts. [score:5]
The same pattern of GFP expression was detected when miR-302/367-GFP expressing lentiviral vectors were used. [score:5]
Regulation of somatic cell reprogramming through inducible mir-302 expression. [score:4]
The miR-302/367 cluster is involved in early embryonic development and expressed in NSCs. [score:4]
A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. [score:3]
A few GFP [+]/NeuN [+] cells were detected within the brains of mice that received control vector+VPA (average cell count: day 7, 0.5; day 14, 8) or miR-302/367 expressing vector (average cell count: day7, 5; day 14, 6) (Fig 4A). [score:3]
The pluripotency genes, Oct4, Sox2 and Nanog increase the expression of miR-302/367 via binding to its promoter. [score:3]
In vitro reprogramming of human astrocytes into neuroblasts was determined by transfection of astrocytes with viral particles that expressed miR-302/367. [score:3]
0127878.g005 Fig 5 (A) Expressions of Oct4 and Nanog genes as pluripotent markers were not detected following miR-302/367+ valproic acid (VPA) treatment. [score:3]
Groups 5 and 6 received focal injections of viral particles that expressed the miR-302/367+GFP cluster and VPA injections as mentioned for groups 1 and 2. For astrocyte transplantation, 300,000 human astrocytes were concentrated in 3 μl of DMEM and transplanted into the mouse striatum two days after transfection. [score:3]
In order to prove in vivo conversion of astrocytes to neuronal cells, we transfected human cultured astrocytes with miR-302/367-GFP expressing viral particles which were subsequently transplanted into the striatum (Fig 6A). [score:3]
Human astrocytes were transduced with the miR-302/367+GFP expressing vector in vitro and then transplanted into mice striata. [score:3]
However, the combination of miR-302/367 and VPA resulted co -expression of DCX [+]/GFP [+] cells at both 7 and 14 dpi (Fig 3) which implied conversion of astrocytes into neuroblasts. [score:3]
0127878.g004 Fig 4 (A) received miR-302/367+GFP expressing vectors. [score:3]
Following in vitro neuronal induction of astrocytes by using the miR-302/367 cluster, some neuroblasts and neurons were detected that did not express GFP. [score:3]
which received both VPA and miR-302/367 expressing vector had higher numbers of GFP [+]/NeuN [+] cells especially at day 14 (average cell number: 39.33) (Fig 4). [score:3]
Morrisey et al. showed that the miR-302/367 cluster alone was enough to reprogram human fibroblasts to iPSCs, while the presence of valproic acid (VPA) as a histone deacetylase inhibitor was necessary for conversion of mouse fibroblasts to iPSCs by the miR-302/367 cluster. [score:3]
Interestingly, in a positive feedback loop miRNA-302/367 enhances the expression of the above mentioned reprogramming factors. [score:3]
Groups 5 and 6 received focal injections of viral particles that expressed the miR-302/367+GFP cluster and VPA injections as mentioned for groups 1 and 2. For astrocyte transplantation, 300,000 human astrocytes were concentrated in 3 μl of DMEM and transplanted into the mouse striatum two days after transfection. [score:3]
At six weeks after transfection of astrocytes with the miR-302/367 cluster, we checked for the expressions of markers of differentiated neurons. [score:3]
We used hematoxylin and eosin staining to assess the possibility of teratoma formation following local injection of the miR-302/367 cluster expressing lentiviral particles. [score:3]
At 24 hours after seeding, astrocytes were infected with GFP or miR-302/367-GFP expressing lentiviral particles and allowed to incubate overnight. [score:3]
The number of GFP [+] cells which expressed NeuN significantly increased in animals treated with the miR-302/367 cluster and VPA, especially at day 14. [score:3]
More than 80% of astrocytes became transduced with miR-302/367 and expressed GFP (Fig 6B). [score:3]
0127878.g007 Fig 7 (A) Human induced neurons expressed neuroblast marker [doublecortin (DCX)] and neuronal markers (TUJ1 and NeuN) at 8 and 10 days post in vitro (DPI) when they received the miR-302/367 cluster. [score:3]
We prepared the miR-302/367 cluster as lentiviral particles which included a GFP expressing sequence (System Biosciences, San Francisco, CA) by transfecting along with a Virapower Lentiviral Packaging Mix (Invitrogen) into 293T cells by the Lipofectamine 2000 Transfection Reagent (Invitrogen). [score:3]
Groups 3 and 4 received focal injection of viral particles that expressed the miR-302/367+GFP cluster. [score:3]
Administration of GFP and miR-302/367 expressing lentiviral particles into the striatum and the distribution of transduced cells. [score:3]
Following in vivo application of miR-302 we checked for the expression of pluripotency markers Oct4 and Nanog and did not detect positive cells. [score:3]
The administration of empty vector (GFP expressing vector) and/or miR-302/367 cluster vector did not cause the conversion of transfected (green) cells into neuroblasts during 2 weeks (Fig 3). [score:3]
The miR-302/367 cluster has been frequently reported as one of the highly expressed microRNAs in pluripotent stem cells [25– 29]. [score:3]
These induced neuroblasts could potentially generate neuronal cells; thus miR-302/367 might be considered a new tool for conversion of glial scar astrocytes to endogenous neuroblasts in repairing lesions for different neurological diseases. [score:2]
miR-302/376 induced neurons might be the result of pluripotent reprogramming of glial cells towards iPSCs and their subsequent differentiation to neuronal cells, or the result of direct conversion of astrocytes into neuroblasts. [score:2]
These results show that neuroblasts can be generated directly from adult human and mouse astrocytes by miR-302/367 -driven induction. [score:2]
Here we have shown that adult human astrocytes could be reprogrammed to neuroblasts by miR-302/367, both in vivo and in vitro. [score:1]
In vivo conversion of engrafted human astrocytes to neuroblasts by miR-302/367. [score:1]
Doublecortin (DCX [+]) cells were detected at 7 and 14 days post-injection (dpi) in animals pre -treated with VPA that afterwards received miR-302/367. [score:1]
Considering the previous reports on the effectiveness of Oct4, Sox2 and Nanog in inducing astrocytes into neurons, this positive loop may explain a possible mechanism for miRNA-302/367 induced neuronal fate from astrocytes [27, 28, 35]. [score:1]
Human astrocytes were converted into neurons by the miR-302/367 cluster in vitro, without pre-treatment with valproic acid (VPA). [score:1]
Here, we have shown high conversion of astrocytes to neuroblasts by miR-302/367 administered in conjunction with VPA. [score:1]
These data showed the neuronal differentiation of cells induced by miR302/367+VPA. [score:1]
Nine days after transduction of astrocytes with miR-302/376, immunofluorescence staining was performed to determine the fate of the engrafted cells. [score:1]
both miR302/367 and empty vector+VPA groups at day 14). [score:1]
Scale bar: 50 μm We showed that application of the miR-302/367 resulted in reprograming of adult mouse human astrocytes into neuroblasts both in vivo and in vitro. [score:1]
This data showed the conversion of induced human astrocytes to neuronal cells by miR302/367 alone. [score:1]
According to Fig 5A, we could not detected any Oct4 and Nanog positive cells in the sections obtained from mice treated with both the miR-302/367 cluster and VPA. [score:1]
0127878.g003 Fig 3Doublecortin (DCX [+]) cells were detected at 7 and 14 days post-injection (dpi) in animals pre -treated with VPA that afterwards received miR-302/367. [score:1]
Following focal administration of the miR-302/367 cluster into the striatum, we studied the fate of transfected cells by specific staining against doublecortin (DCX) as a neuroblast marker. [score:1]
Two months after miR-302/367 injections, the animals were perfused with PBS and paraformaldehyde, respectively and the sections were counterstained with hematoxylin and eosin. [score:1]
Although the astrocytes were the major transfected cells after in vivo injection of viral particles, other cell types might also receive miR-302/367 and undergo reprogramming. [score:1]
In vitro conversion of astrocytes to neuron-like cells by miR-302/367. [score:1]
In addition, we showed that human astrocytes could reprogram into neurons by the miR-302/367 cluster alone in neuronal differentiation medium. [score:1]
A number of cells transduced with the miR-302/367+GFP cluster following valproic acid (VPA) pre-treatment showed neuronal fate as determined by immunostaining against NeuN. [score:1]
Cells were prepared for electrophysiological recording at six weeks after transfection with the miR-302/367 cluster. [score:1]
We observed DCX, TUJ1 and NeuN positive cells in the miR-302/367+GFP treated cultures which implied conversion of astrocytes into neuroblasts and neuronal cells. [score:1]
miR-302/367 and valproic acid (VPA) converted the transducted cells into neuroblasts. [score:1]
Human astrocytes transduced with miR-302/367 produced neuroblasts in vitro as well as in vivo when engrafted into the adult mouse brain. [score:1]
Two months after injection of viral particles that expressed miR-302/367 and VPA, the striata areas were investigated for teratoma formation. [score:1]
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5
[+] score: 146
Among the targets predicted by the search programs of TargetScan, miRanda and miRDB, we found Rad52 was the theoretical target gene of miR-302a (Figure 3A). [score:7]
Our results provide evidence that miR-302a synergistically increases the sensitivity of AML cells to VP-16 and demonstrate that miR-302a downregulates Rad52 to inhibit tumor growth and chemoresistance, in part via the AKT/Gsk-3β/β-catenin signaling cascade. [score:6]
It has been reported that miR-302a involves in maintaining stemness of hESCs [10, 11, 12], suppressing tumor cell proliferation and inducing cancer cell apoptosis by directly targets Rad52 and AKT1 [13]. [score:6]
Downregulation of Rad52 by miR-302a suppresses cell proliferation and chemoresistance, in part by activating the AKT/Gsk-3β/β-catenin cascade. [score:6]
We found that the expression of the reporter gene in the recombinant plasmid containing RAD52 3’UTR was significantly attenuated in miR-302a -overexpressing cells and could be restored in miR-302a-mutant cells. [score:5]
To search for the miR-302a target gene involved in VP-16 sensitivity, we used several bioinformatics methods to help identify the target human genes of miR-302a. [score:5]
The present in vitro and in vivo study assays presented herein demonstrate that miR-302a down-regulates the Rad52 gene expression. [score:5]
Next, we found that the AKT/β-catenin signaling was inactivated in VP-16 treatment cells, and that overexpression of miR-302a reversed the decreased expression of β-catenin by promoting Rad52/AKT/β-catenin signaling (Figure 6C and Supplementary Figure 5C). [score:5]
Rad52 is a direct target of miR-302a. [score:4]
In this study, we showed that miR-302a was downregulated in VP-16 -treated AML cells. [score:4]
After 48 h of transfection, MTT assays showed that overexpression of miR-302a led to enhanced inhibition of proliferation in comparison with the control (Figure 2B and Supplementary Figure 1). [score:4]
In addition, we investigated the expression levels of β-catenin target-genes after overexpression of miR-302a by western blotting assay (Supplementary Figure 6). [score:4]
Taken together, these results reveal that miR-302a might enhance VP-16 sensitivity by targeting Rad52 through regulating the AKT/Gsk3β/β-catenin pathway. [score:4]
The mechanism is direct regulation of RAD52 by miR-302a, leading to regulation of the intrinsic AKT/Gsk3β/β-catenin pathway in AML. [score:4]
The data presented herein highlight the potential clinical utility of miR-302a levels as a valuable biomarker that reflects the expression of miR-302a in human AML samples. [score:3]
Overexpression of miR-302a enhances the sensitivity to VP-16 in HL-60 Cell. [score:3]
Figure 1 (A) qRT-PCR analysis of miR-302a expression in MNCs from healthy people, AML patients and AML cell lines. [score:3]
Overexpression of miR-302a failed to reduce the total amount of AKT protein. [score:3]
Interestingly, miR-302a was significantly down-regulated in leukemic cells in both primary AML samples and cell lines, as compared with that of MNCs (Mononuclear cells) isolated from the peripheral blood of healthy volunteers (Figure 1A). [score:3]
Overexpression of miR-302a enhances the sensitivity to VP-16 in AML cell lines. [score:3]
The potential mechanism mediating these effects may be associated with the capacity of miR-302a to inhibit cell growth and induce cells apoptosis in HL-60 and U937 cells. [score:3]
This indicates that miR-302a enhances the tumor-suppressive effect of VP-16 in a xenograft mo del of human AML. [score:3]
These findings proved miR-302a could serve as a therapeutic target or diagnostic/prognostic marker for acute myeloid leukemia therapy. [score:3]
Figure 3 (A) Schematic representation of Rad52 3′-UTRs showing putative miR-302a target site. [score:3]
To validate that miR-302a can directly bind to and regulate the levels of Rad52 mRNA through the predicted binding sites, we altered bases of Rad52 in the putative miR-302a binding site and found that the mutant 3’UTRs were completely refractory to miR-302a -mediated luciferase reporter repression in HL-60 cells (Figure 3C and Supplementary Figure 2). [score:3]
Twenty nude mice (BALB/c-nu; 5 weeks old) were divided randomly into two cohorts and inoculated in the right flank with 2x 10 [6] HL-60 cells that stably expressed MSCV vector or MSCV-miR-302a. [score:3]
In the present study, miR-302a can suppress cell proliferation and decrease VP-16 resistance by activating AKT/β-catenin signaling. [score:3]
As shown in Figure 3, Rad52 is the specific target of miR-302a. [score:3]
miR-302a may serve as a therapeutic target or diagnostic/prognostic marker for leukemia therapy. [score:3]
Figure 6 (A) HL-60 cell lines were transfected with miR-302a or miR-302a and Rad52 overexpression vector respectively. [score:3]
In summary, our study have found miR-302a enhance the sensitivity of acute myeloid leukemia cell lines to VP-16 by targeting Rad52 and in part via the AKT/Gsk-3β/β-catenin signaling cascade. [score:3]
Reduced expression of miR-302a in AML patients and cell lines. [score:3]
Increased expression of miR-302a upon transfection were determined by qRT-PCR (Figure 2A and Supplementary Figure 1). [score:3]
Notably, within 3 weeks of treatment, miR-302a mice that received VP-16 had pronounced inhibition of tumor growth compared to MSCV mice that received the agent or miR-302a mice that received the vehicle control (Figure 5A–5C and Supplementary Figure 4). [score:2]
In order to investigate potential alterations in the expression pattern of miR-302a in AML, the expression levels of miR-302a were measured by qRT-PCR in 20 patients with AML and in the AML HL-60 and U937 cell lines, then compared with the expression levels of miR-302a in 8 healthy volunteers. [score:2]
the expression level of miR-302a was measured by qRT-PCR. [score:1]
The synthetic mimics of miR-302a along with scrambled oligonucleotides were purchased from Genepharma (Shanghai, China). [score:1]
To the best of our knowledge, this is the first study to demonstrate that miR-302a levels could be a valuable biomarker and an important prognostic factor for human AML. [score:1]
When the tumors were palpable, the MSCV and miR-302a mice were subdivided into two groups and treated with either vehicle (water) or VP-16 (20 mg/kg). [score:1]
As well, we found miR-302a sensitive AML cell lines to VP-16 in nude mice in vivo. [score:1]
To investigate the underlying mechanisms by which miR-302a influence AML VP-16 sensitivity, we used bioinformatic methods to predict the possible target genes. [score:1]
Figure 2 (A) HL-60 was transfected the miR-302a or negative control by Lipo2000. [score:1]
However, there is still little known about the potential role of miR-302a in VP16 -based AML chemotherapy and further research is needed. [score:1]
And the sequences of miRNA and siRNA were as follows: NC: AGGUAGUGUAAUCGCCUUG; siRad52-1: UGAGAUGUUUGGUUACAAU; siRad52-2: CCCUGAAGACAACCUUGAA; miR-NC: UUGUACUACACAAAAGUACUG miR-302a mimic: UAAGUGCUUCCAUGUUUUGGUGA. [score:1]
MiR-302a sensitizes xenograft tumors to chemotherapeutic drug in vivoTo evaluate the effect of miR-302a on the sensitivity of AML cells to a chemotherapeutic drug (VP-16) in vivo, HL-60 and U937 cells stably overexpressing MSCV vector or MSCV-miR-302a were injected into the right flanks of nude mice. [score:1]
Here, in this research we identified for the first time that miR-302a exert effective functions on improving AML VP-16 sensitivity. [score:1]
Figure 5MiR-302a sensitizes xenograft tumors to a chemotherapeutic drug in vivo (A) in vivo growth rates of tumor volume of miR-NC, miR-302a, miR-NC+VP16 and miR-302a+VP-16 xenograft tumor grown in nude mice. [score:1]
We found that miR-302a tuned-down both the proliferation and growth of AML cells. [score:1]
In this study, the potential alterations in the expression pattern of miR-302a were evaluated in AML patient and in the AML cell lines. [score:1]
To evaluate the effect of miR-302a on the sensitivity of AML cells to a chemotherapeutic drug (VP-16) in vivo, HL-60 and U937 cells stably overexpressing MSCV vector or MSCV-miR-302a were injected into the right flanks of nude mice. [score:1]
We also identified the potential value of miR-302a in AML cell lines. [score:1]
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[+] score: 105
[16] Figure 3b shows the expression levels of miR-302a and its targets CDK2 and BMI-1. MiR-302a was significantly upregulated in comparison with control cells, and CDK2 and BMI-1 were downregulated, after treatment of AGS cells with SAHA and DZNep. [score:11]
Tumor-suppressor miRNAs such as miR-1246, miR-302a and miR-4448 are activated and suppress their cancer-related target genes, thus inducing apoptosis and G1/S arrest of cancer cells and inhibiting their migration. [score:9]
Our results suggest that miR-302a acts as a tumor suppressor and suppresses the cancer-stemness signature in cancer cells by suppressing target genes such as CDK2 and BMI-1, although it has an important role in maintaining stemness in pluripotent cells such as ES cells and iPS cells. [score:9]
miR-1246, miR-302a and miR-4448 suppress their target genes upon treatment with SAHA and DZNep in cancer cellsA recent study has shown that dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A), a Down syndrome -associated protein kinase, is a target of miR-1246. [score:8]
miR-302a and miR-4448 are upregulated by SAHA and DZNepWe also found that miR-302a and miR-4448 were most upregulated by treatment of AGS cells with SAHA, and by treatment of both AGS and HepG2 cells with DZNep, respectively (Table 1). [score:7]
Treatment of AGS cells with DZNep and SAHA suppressed CDK2 and BMI-1, which were recently identified as the targets of miR-302, and induced cell cycle (G1/S) arrest. [score:5]
miR-1246, miR-302a and miR-4448 suppress their target genes upon treatment with SAHA and DZNep in cancer cells. [score:5]
[21] Here we have shown for the first time that, in cancer cells, miR-1246 is a common target of EZH2 inhibitors and that miR-302a and miR-4448 are activated by SAHA and DZNep. [score:5]
miR-302a and miR-4448 are upregulated by SAHA and DZNep. [score:4]
Cyclin -dependent kinase 2 (CDK2) and BMI-1 polycomb ring finger oncogene (BMI-1), both of which are known to be cell cycle regulators, have been identified as targets of miR-302. [score:4]
Upregulation of miR-302a and miR-4448 by treatment with SAHA and DZNep was confirmed by quantitative RT–PCR (Figures 2b and c). [score:4]
We also found that miR-302a and miR-4448 were most upregulated by treatment of AGS cells with SAHA, and by treatment of both AGS and HepG2 cells with DZNep, respectively (Table 1). [score:4]
In the present study, we focused on three miRNAs— miR-1246, miR-302a and miR-4448—which were robustly upregulated by SAHA and DZNep treatment in AGS and HepG2 cells. [score:4]
Recent studies have shown that miR-302 is the major miRNA found in human embryonic stem cells and iPS cells, and that induction of miR-302 expression reprograms somatic cells into a pluripotent stem cell-like state. [score:3]
As EZH2 is the catalytic subunit of polycomb repressive complex 2, which mediates epigenetic gene silencing by trimethylating histone H3 lysine 27, suppression of EZH2 by SAHA and DZNep may induce transcriptional activation of miR-1246, miR-302a and miR-4448 in cancer cells. [score:3]
These findings indicate that binding of EZH2 to the promoter regions of miR-1246, miR-302a and miR-4448 was inhibited in cancer cells by treatment with SAHA and DZNep. [score:3]
Binding of EZH2 to the promoter regions of miR-1246, miR-302a and miR-4448 is inhibited by SAHA and DZNep. [score:3]
Binding of EZH2 to the promoter regions of miR-1246, miR-302a and miR-4448 is inhibited by SAHA and DZNepFinally, we performed the ChIP assay with antibodies against EZH2 and p53 to clarify the mechanism responsible for regulation of these miRNAs by histone-modifying drugs. [score:3]
Expression levels of genes were analyzed by TaqMan quantitative RT–PCR assay for miR-1246, miR-302a, Oct3/4 and Sox2 (Applied Biosystems) in accordance with the manufacturer's instructions. [score:2]
ChIP assay revealed that binding of EZH2 to the promoter regions of miR-1246, miR-302a and miR-4448 was inhibited by SAHA and DZNep. [score:2]
14, 15 MiR-302 has been reported to inhibit the tumorigenicity of human pluripotent stem cells and the proliferation of cervical carcinoma cells. [score:2]
miR-302a forward: 5′-GGGTAAAAGGCAGGGACTTC-3′, miR-302a reverse: 5′-CAGACCCACCCAGGATCATA-3′. [score:1]
U6 was used as an internal control for miR-1246 and miR-302a. [score:1]
Chromatin around the miR-302a promoter region that was immunoprecipitated with EZH2 antibody was significantly reduced by treatment with SAHA and DZNep in AGS cells (Figure 6b). [score:1]
miR-302a forward: 5′-GGGTAAAAGGCAGGGACTTC-3′, miR-302a reverse: 5′-CAGACCCACCCAGGATCATA-3′. [score:1]
[24] Thus, concurrent silencing of CDK2 and BMI-1 by miR-302a may synergistically bring about G1/S cell cycle arrest of AGS cells. [score:1]
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[+] score: 87
In cells that express active miR-302a, the miR-switch will repress the translation of the downstream puroR gene. [score:5]
mirVana miRNA Mimic, Negative Control #1, mirVana miRNA Inhibitor Negative Control, hsa-miR-302-5p mimic (Pre-miR/Anit-miR ID:MC12557), and 302a-5p inhibitor (Pre-miR/Anti-miR ID:MH12557) were purchased from Applied Biosystems and used at the stated picomole amount. [score:5]
When regulating the puroR translation with miR-302a switch, we observed significant cell toxicity in hiPSCs but not in differentiated mDA neuronal cultures (Supplementary Fig. 5b). [score:4]
Therefore, we hypothesised that placing a simple puromycin selection circuit using puromycin-resistant mRNA (puroR) under the translational regulation of miR-302a switch would allow for automated elimination of residual hiPSCs without the need for cell sorting (Fig. 6a). [score:4]
We next co -transfected varied amounts of the miRNA inhibitor in 201B7 and a miRNA mimic in HeLa cells to simulate the dynamic range of the miR-302a switch. [score:3]
We measured the expression of miR-302a and miR-367 and then expression of key pluripotent-, neuroectoderm- and mDA -associated mRNAs. [score:3]
Moreover, the gradual recovery in translational efficiency in the representative dot plots from days 5, 7 and 9 (Fig. 2b), indicates that the level of active miR-302a in the cells gradually decreased during differentiation. [score:3]
We next checked whether the repression on translation efficiency by the miR-302a switch was indeed specific to the miR-302a-5p activity. [score:3]
We co -transfected 201B7 cells with 15 pmol of either a control (green bar) or miR-302a specific (grey bar) miRNA inhibitor (Supplementary Fig. 1b). [score:3]
To our knowledge, the miR-302a switch is the only methodology that identifies, purifies, and/or eliminates target pluripotent stem cells based on intracellular signatures. [score:3]
We first confirmed the specific and high level of expression of both hsa-miR-302a-5p (herein referred to as miR-302a) and hsa-miR-367-3p (herein referred to as miR-367) in three feeder-free human iPSC lines (201B7, 1231A3, and 1383D7). [score:3]
These results indicate miR-302a activity regulates the miR-302a switch specifically. [score:2]
The miR-302/367 cluster expression is necessary for maintaining the self-renewal of pluripotent cells and also an upstream regulator for many classical stem cell markers such as TRA-1-60, placental-like alkaline phosphatase and the intracellular transcription factors OCT3/4 and NANOG, which can only be measured in fixed or lysed cells 25 26. [score:2]
The target sites for hsa-miR-302a-5p and 367-3p are underlined and in bold in the primer list (Supplementary Table 1). [score:2]
We next tested our miR-302a switch on another hiPSC-derived differentiated cell type. [score:1]
miR-302a switch distinguishes hiPSCs and hiPSC-derived differentiated cells. [score:1]
Thus, as a next step, we moved from single- to co-cultured cell populations to check whether miR-302a switch could isolate hiPSCs from differentiated cells in a mixed culture. [score:1]
These data suggests miR-302a switch can detect partially differentiated cells (e. g., the cells after 9 days of differentiation in Fig. 2b,c). [score:1]
miR-302a switch sorted cells (302-neg) effectively removed spiked hiPSCs as no teratomas were observed in all of the tested animals (Fig. 5b,c). [score:1]
miR-302a switch-sorted hiPSC-spiked co-cultures could prevent teratoma formation in SCID mice. [score:1]
miR-302a switch effectively separates hiPSCs and hiPSC-derived differentiated cell sIn addition to checking standard culture lines and primary cells, we also tested our miR-302a switch on hiPSC-derived differentiated cells. [score:1]
Detection sensitivity for spiked hiPSC cells with miR-302a switch. [score:1]
Generation of live-cell reporters to detect pluripotency-specific miR-302/367 activity. [score:1]
We believe removing residual miR-302a-responsive cells at earlier time points (e. g., day 5 in Fig. 2d) and re-culturing the negative fraction (miR-302a non-responsive cells) may lead to greater differentiation efficiencies. [score:1]
Sorting of partially differentiated mDA cells using the miR-302a switch. [score:1]
These results further confirm that miR-302a switch can reproducibly distinguish hiPSCs and their differentiated cells. [score:1]
Thus, miR-302a switch can assay the differentiation dynamics and optimal condition and clone for directed differentiation. [score:1]
Thus, we can easily exclude both untransfected cells and miR-302 -positive/undifferentiated cells. [score:1]
Again, proving that miR-302a switch is specific to pluripotent stem cells, there was a complete overlap of 201B7-derived spontaneously differentiated cells transfected with either Ctrl-miR or miR-302a switch (Fig. 2a), suggesting that miR-302a switch distinguishes hiPSCs and differentiated cells. [score:1]
From these results, we decided to focus on miR-302a switch for the following reasons: (1) From three independent experiments, the percentage of hiPS cells responsive to the miR-302a switch (positive = 97.05%, SEM ± 3.18) than those that responded to the miR-367 switch (pos = 81.45%, SEM ± 19.97) (Fig. 1c); (2) When measuring the translational efficiency from the dot plots the miR-302a switched -transfected hiPS cells gave a higher fold-change than miR-367 switch -transfected cells (Fig. 1d); (3) And the miR-302a switch could completely separate 201B7 from NHDF and HeLa cells, whereas the miR-367 switch did not (Fig. 1e). [score:1]
RIGHT PANELS Representative dot plot of the 201B7-DD″ after 14 days differentiation transfected with either Ctrl-miR (black) or miR-302a (green) switches. [score:1]
miR-302a switch sorting on partially differentiated mDA neuronal cultures. [score:1]
In contrast cells unresponsive to miR-302a switch will survive (Fig. 6a). [score:1]
2 × 10 [5] of non-sorted mDA D20 cells, Ctrl-miR switch-sorted hiPSC-spiked mDA culture, and miR-302a switch-sorted hiPSC-spiked mDA culture were injected into both testes of 4 animals for each group. [score:1]
201B7 cells transfected with either miR-302a (green, Fig. 1c) or miR-367 (purple, Fig. 1c) switches are spatially resolved from cells transfected with miR switch containing no miRNA antisense sequence (Ctrl-miR switch, black dots), whereas most HeLa cells transfected with the three switches overlapped each other (Fig. 1c). [score:1]
For example, we consistently observed miR-302a switch-responsive cells up to day 9 of the mDA protocol despite being TRA-1-60 negative (Fig. 2d,e). [score:1]
As a proof of principle that our miR-302a switch can be used to make cultures safer for transplantation, we deliberately suspended the mDA differentiation of hiPSC cells at day 4 (cells after 4 days of differentiation contain undifferentiated and/or partially differentiated cells in our mDA induction protocol). [score:1]
We successfully detected hiPSCs at all the spiked levels with miR-302a switch. [score:1]
We generated in vitro transcribed modRNA that encoded miR switches that can sensitively and dynamically respond to the activities of miR-302a and miR-367 in living cells. [score:1]
miR-302a and 367 switches specifically detect hiPSC cells. [score:1]
Sensitive detection of spiked-hiPSCs using miR-302a switch. [score:1]
miR-302a switch effectively separates hiPSCs and hiPSC-derived differentiated cell s. Monitoring differentiation dynamics and efficiency using miR-302a switch. [score:1]
Furthermore, when using the same differentiation protocol on a differentiation-defective version of 201B7, 201B7-DD″ (see methods and Supplementary Fig. 2), after 14 days we could detect residual differentiation resistant cells (14.6 ± 3.4%) when transfecting miR-302a switch (Fig. 2a). [score:1]
Having succeeded in showing cell-specific toxicity with miR-302a-puroR switch in single culture, we then aimed to eliminate hiPSCs co-cultured with differentiated mDA cells. [score:1]
Transplantation of miR-302a switch-sorted cells. [score:1]
hiPSCs were partially differentiated through the mDA differentiation protocol for 4 days and passaged to a new 6-well plate for reverse transfection of miR-302a switch and sorted into 302-pos and 302-neg fractions for analysis or recultured for later alkaline phosphatase staining. [score:1]
tagBFP fluorescence for day 12 mDA cells spiked with 5%, 2.5%, 0.5%, 0.25% and 0.05% hiPS cells and transfected with 90 ng of miR-302a switch mRNA and 180 ng of tagBFP, and stained with anti-TRA-1-60 antibody before flow cytometry (Supplementary Fig. 4). [score:1]
To show that we could effectively remove the spiked hiPSC cells, we transplanted the sorted miR-302-neg cells into the testes of SCID mice for allogeneic engraftment. [score:1]
In addition to checking standard culture lines and primary cells, we also tested our miR-302a switch on hiPSC-derived differentiated cells. [score:1]
It is also noteworthy that our miR-302 switch system eliminates undesired cells based on positive selection of fluorescent-reporter mRNAs (i. e., we can isolate fluorescent-reporter -positive (miR302 -negative) cells). [score:1]
Interestingly, TRA-1-60-neg cells also went on to form ALP [+] colonies (21 ± 3 colonies), illustrating the high sensitivity of miR-302a switch, which can be used to remove harmful overgrowing cells. [score:1]
As expected, the 302-neg sorted fraction contained a reduced level of both miR-302a and miR-367 relative to 302-pos sorted fraction (Fig. 4b). [score:1]
A major advantage of our strategy is that we can place any gene as the reporter on the miR-302a switch. [score:1]
After 14 days, like the results for spontaneously differentiated cells, the mDA neurons transfected with miR-302a switch overlapped with those transfected with Ctrl-miR switch (Fig. 2b, D14). [score:1]
These cells were then transfected with miR-302a switch and underwent FACS the following day to separate 302-pos and 302 -negative (302-neg) cells (see scheme in Fig. 4a). [score:1]
If comparing miR-302a-responsive cells over time, the 1231A3 hiPSCs also differentiate quicker than 201B7 hiPSCs. [score:1]
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[+] score: 72
Lin et al. (2011) reported that miR-302 targeted cosuppression of four “epigenetic” regulators, lysine demethylase KDM1 (also known as LSD1/AOF2), AOF1, p66/MECP1, and MECP2 [57]. [score:6]
Lin et al. reported that maintaining slowly proliferating adhesive stem cells will require inhibition of cell cycle by miR-302/367 that targets cyclin D1 and cyclin -dependent kinases 2/4/6 (Cdk2, Cdk4 and Cdk6) [55]. [score:5]
Lipchina et al. reported that miR-302/367 cluster promotes BMP signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1 [59] (Figure 1). [score:5]
Liao et al. reported that the miR-302/367 cluster enhanced somatic cell reprogramming (SCR) by accelerating an MET through targeting TGF β type II receptor (TGFbR2) and increased E-cadherin expression [58]. [score:5]
The first report of targets of miR-302 in ES and iPS cells was regarding cell cycle regulators. [score:4]
This group confirmed that the presence of a signature group of miRNAs that is upregulated in both iPS and hES cells, such as the miR-302/367 and miR-17/92 clusters. [score:4]
This group showed that defined hES cells-enriched miRNA groups (miR-302, miR-17, miR-515 families, and the miR-371-373 cluster) were downregulated “rapidly” in response to differentiation. [score:4]
Card et al. showed that Oct4/Sox2-regulated miR-302 targeted mRNA encoding cyclin D1 in hES cells [54] (Figure 1). [score:4]
Li et al. reported that not only miR-302 but also miR-93 targets mRNA encoding TGFbR2 to enhance generation of iPS cells [60]. [score:3]
The miR-302 -transfected cells expressed key ES cell markers such as Oct3/4, SSEA-3/4, Sox2, and Nanog but also had a highly demethylated genome similar to a reprogrammed zygotic genome. [score:3]
Targeting TGF β signaling by miR-302 may reprogram cells toward generation of iPS and mirPS cells through induction of mesenchymal-epithelial transition (MET), the acquisition of intercellular adhesion. [score:3]
This group firstly reported that the miR-302 family members (miR-302s) were expressed most abundantly in slowly growing human ES cells and “quickly” decreased after cell differentiation and proliferation. [score:3]
Later miRNA profiling studies reproduced that miR-302 was essentially expressed specifically in ES and iPS cells and lost upon differentiation and proliferation. [score:3]
One arising question was what factors were targeted by miR-302. [score:3]
Thereafter, Barroso- del Jesus et al. (2009) released perspectives regarding the miR-302/367 cluster as a potential stemness regulator in ES cells [51]. [score:2]
This work showed an extremely higher efficiency of ES cell-like colony formation with ES cell-like morphology and expression of markers using miR-302/367 cluster compared to OSKM-iPS. [score:2]
MicroRNA-302/367 Cluster Targets Multiple Factors Involving Epithelial-Mesenchymal and G1-S Transitions. [score:2]
Sequencing of RNA transcripts revealed that a pre-miRNA cluster encoded five miRNAs including miR-302a, -302b, -302c, -302d (miR-302s), and miR-367, termed miR-302/367 cluster. [score:1]
This work explained how the miR-302 overcame the G1-S arrest at the G1-S transition. [score:1]
Lin et al. reported that miR-302 reprogrammed human skin cancer cells into a pluripotent ESC-like state [50]. [score:1]
These works using miR-302/367 showed that blocking mesenchymal TGF β signaling and maintenance of BMP signaling were required for generation and maintenance of iPS cells. [score:1]
Thus, CCN2/CTGF may be useful as a growth factor supporting cellular reprogramming through induction of miR-302. [score:1]
Poleganov et al. reported that human fibroblasts and “blood-derived endothelial progenitor” cells were efficiently reprogrammed by transduction of nonmodified miR-302/367 cluster in a single vector leading to immune evasion [62]. [score:1]
This group used a combination of miR-200c plus miR-302 s and miR-369 s family of miRNAs without using OSKM factors. [score:1]
So far, established markers of ES and iPS cells are SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, Oct-4 (Pou5f1), Nanog, Sox2, Klf4, MycN, Lin28, Cripto, Fbx15, Dnmt3b, Fgf4, Gdf3, Rex1, miR-200c, miR-302 family, miR-369-3p, and miR-369-5p. [score:1]
Anokye-Danso et al. reported miRNA-302/367 -mediated reprogramming of mouse and human somatic cells to pluripotency [61]. [score:1]
Combination of miR-200c Plus miR-302 s and miR-369 s Family. [score:1]
In this study, the number of colonies with ES cell-like morphology per 100,000 cells was 10396 cells using miR-302/367 and only 3 with OSKM in this work. [score:1]
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[+] score: 70
In this study, the members of the endogenous miR-302/372/373/520 family were found to suppress ZEB1 transcription factor in PC-3 cells and finally activate the expression of E-cadherin through a similar regulatory pathway induced by dsEcad640. [score:6]
However, when dsEcad215, dsEcad302, dsEcad640, or members of miR-302/372/373/520 family act on ZEB1 mRNA and suppress the expression of ZEB1, the transcriptional repression of E-cadherin is alleviated (lower panel). [score:5]
Thus, the members of the miR-302/372/373/520 miRNA family were revealed to be able to reduce the expression of ZEB1 by targeting its CDS. [score:5]
Increase of E-cadherin expression via suppression of the ZEB1 transcription factor by miR-302/372/373/520 family miRNAs. [score:5]
Our result might account for a pathway to gain pluripotency by linking the mirPS induction pathway by miR-302 and that to induce pluripotent state of FAB-SCs by E-cadherin upregulation. [score:4]
Microarray profiles of gene expression by the transfection of miR-302/372/373/520 family members and dsEcad640. [score:3]
Microarray analyses of gene expression profiles by the transfection of dsEcad640 and members of miR-302/372/373/520 family miRNAs. [score:3]
of a one-sided K-S test for seed -dependent off-target effects is as follows: transcripts with complementary seed sequences of the opposite strand of dsEcad640, P = 0.999; those of miR-302a, P = 0.266; those of miR-372, P = 0.449; those of miR-373, P = 0.953; those of miR-520c, P = 0.031; those of miR-520f, P = 0.998. [score:3]
The mean expression levels of the transcripts that have common seed-complementary sequences, AGCACUU, to dsEcad640 and miR-302/372/373/520 miRNA family members in their 3′UTR were apparently reduced by the transfection with dsEcad640, miR-302a, miR-372, miR-373, and miR-520c duplexes (Figure S1). [score:3]
In the promoter analyses, miR-302a, miR-372, miR-373, miR-520c, and miR-520f duplexes showed the effects to increase the expression of proE-cad178-Luc and proE-cad670-Luc (Figure 6B, and D). [score:3]
The seed complementary sites in the ZEB1 CDS were found to be target sites of seed -dependent silencing by dsEcad215, dsEcad640, and miR-302/372/373/520 family members (Figure 4A). [score:3]
Furthermore, the members of miR-302a/372/373/520 family miRNAs, which contain the same seed sequence with dsEcad640, showed parallel effects on ZEB1 expression, advocating the possibility that such a mechanism is also functional in vivo. [score:3]
A noncoding RNA cluster containing mir-302b, mir-302c, mir-302a, and mir-367 are known to be expressed most abundantly in human ES cells, and quickly decrease after cell differentiation and proliferation [49]. [score:3]
of a one-sided K-S test for seed -dependent off-target effects are as follows: transcripts with seed-complementary sequences of dsEcad640, P≤10 [−59]; those of miR-302a, P≤10 [−45]; those of miR-372, P≤10 [−20]; those of miR-373, P≤10 [−39]; those of miR-520c, P≤10 [−40]; those of miR-520f using common seed sequence, P≤10 [−14]; miR-520f using own seed sequence, P≤10 [−57]. [score:3]
To assess the parallel genome-wide regulation by dsEcad640 and the members of miR-302/372/373/520 family, microarray analyses were performed using PC-3 cells transfected with each of the miR-302a, miR-372, miR-373, miR-520c, and miR-520f duplexes, as well as with dsEcad640 at 24 hour. [score:2]
These results indicate that miR-302/372/373/520 family members and dsEcad640 show analogous genome-wide gene regulation due to the common seed sequence. [score:2]
The miR-302a duplex is composed of miR-302a and miR-302*; the miR-373 duplex, miR-373 and miR-373*; and the miR-520c duplex, miR-520c-5p and miR-520c-3p. [score:1]
The effects of dsEcad215, dsEcad302, dsEcad640, miR-302a, miR-372, miR-373, miR-520c, miR-520f, and siZEB1_CDS were determined using stably transfected cells with each luciferase reporter, and shown as Renilla/firefly. [score:1]
Microarray profiles of transcripts containing common seed-complementary sequences of dsEcad640 and members of miR-302/372/373/520 family by the transfection of (A) dsEcad640, (B) miR-302a duplex, (C) miR-372 duplex, (D) miR-373 duplex, (E) miR-520c duplex, and (F) miR-520f duplex. [score:1]
Microarray profiles of transcripts containing variable seed-complementary sequences of the opposite strands in their 3′UTRs by the transfection of (A) dsEcad640, (B) miR-302a duplex, (C) miR-372 duplex, (D) miR-373 duplex, (E) miR-520c duplex, and (F) miR-520f duplex. [score:1]
The transcripts of ZEB1, MED8, MTPN, LATS2, and RAB31 possess seed-complementarities to either of dsEcad640, miR-302a, miR-372, miR-373, miR-520c, and miR-520f. [score:1]
These results suggest that members of the miR-302/372/373/520 miRNA family activate E-cadherin transcription via repression of ZEB1 transcriptional factor in an analogous fashion to dsEcad640. [score:1]
The silencing activities by dsEcad640, miR-302a, miR-372, miR-373, miR-520c, and miR-520f on wild-type pLuc-CDS (WT), pLuc-CDS-m640-1, -2, and -1+2 are shown as Renilla/firefly. [score:1]
Chemically synthesized dsEcad640, miR-302a, miR-372, miR-373, miR-520c, and miR-520f duplexes were transfected into PC-3 cells. [score:1]
Then, the effects on ZEB1 CDS were determined by the transfection of miR-302a, miR-372, miR-373, miR-520c, and miR-520f duplexes along with pLuc-CDS and pGL3-Control into PC-3 cells (Figure 1). [score:1]
The transfection of the mir-302 cluster into human Colo and PC-3 cells has been shown to generate ES-like cells, known as miRNA -induced pluripotent stem (mirPS) cells [50]. [score:1]
MiR-302a, miR-372, miR-373, miR-520c, miR-520f miRNA duplexes were synthesized to form the same sequences and structures described in miRBase [51]. [score:1]
Table S2 List of the increased and decreased genes that have common seed-complementary sequences to dsEcad640 and miR-302/372/373/520 miRNA family members. [score:1]
The seed sequence of dsEcad640 antisense strand (AAGUGCU) is same as those of the miR-302/372/373/520 miRNA family members, miR-302a, miR-372, miR-373, miR-520a-3p, and miR-520f, although the seed sequence of miR-520f is shifted by 1 nt to 1–7 nt from 2–8 nt (Figure 1B). [score:1]
The seed sequences of dsEcad640 sense strand, miR-302*, the opposite strand of miR-372, miR-373*, miR-520c-5p, and the opposite strand of miR-520f, are different (Figure 1B). [score:1]
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[+] score: 68
Taken together, miR-302/367 expression induces global demethylation and suppresses NR2F2, two events that indirectly activate Oct4 expression, which in turn elevates miR-302/367 levels. [score:8]
The use of a miRNA expression vector, such as the intronic miRNA expression system, provides a simple and safe way to generate iPSCs due to the fact that no oncogene is required for successful reprogramming and, in the case of miR-302/367, only a single transcript is transfected rather than the simultaneous transfection of multiple genes, whose expression would be difficult to consistently maintain in iPSCs. [score:7]
Among the highly expressed ESC-specific miRNAs, miR-302/367 is highly expressed in early embryonic development and then rapidly declines after differentiation [12, 13]. [score:6]
This, in combination with the observation of high miR-302/367 expression in the early embryo followed by a rapid decrease upon differentiation, strongly suggests that miR-302/367 serves as upstream pluripotency regulator to modulate the expression of Oct4, Sox2, Nanog, and other embryonic transcription factors. [score:6]
Given that miRNA can target several to hundreds of genes, the inhibition of multiple factors and pathways likely initiates miR-302/367 reprogramming. [score:5]
In hESCs, NR2F2 expression begins with differentiation and conversely correlates with the expression of Oct4 and miR-302/367. [score:5]
miR-302/367 also directly targets NR2F2, a member of the nuclear orphan receptor family of transcriptional factors and a negative regulator of Oct4 [25]. [score:5]
Studies from Lin and colleagues showed that overexpression of mir-302/367 (approximately 1.1- to 1.3-fold as compared with normal hESCs) leads to global demethylation and coexpression of Oct4, Sox-2, and Nanog in human iPSCs [20, 27]. [score:4]
Also, miR-302/367 targets several cell cycle regulators. [score:4]
Studies have also shown that Oct4, Nanog, and Sox2 bind to the promoter regions of miR-302/367 and increase its expression level [26]. [score:3]
miR-302/367 targets multiple epigenetic factors, leading to global demethylation. [score:3]
A similar study using RUES2 cells confirmed that transfection with miR-302 elevates Oct4 and Nanog expression [14]. [score:3]
We found that the miR-302 may also target TGFBR2 and RHOC, supporting the possibility that miR-302/367 has the same effect on MET as miR-302b/327. [score:3]
This reciprocal cycle increases cellular levels of miR-302/367 and Oct4, which leads to the co-activation of other transcription regulators, such as Sox2 and Nanog. [score:2]
Based on evidence of the successful establishment of iPSC lines using a miRNA -mediated strategy, it seems that ESC-specific miRNA, especially the miRNA-302/367 family, can induce reprogramming events similar to those of Yamanaka factors. [score:1]
Moreover, instead of the miR-302 family alone, it has been argued that successful reprogramming by mature miRNAs always requires the combination of miR-302 s, miR-200c, and miR-369, while several miRNA -mediated iPSC lines have been generated with only miR-302 or miR-302/367 [20, 22]. [score:1]
Numerous miRNA -mediated iPSC lines have been subsequently developed with either miR-302/367 or a combination of miR-302 and other miRNAs from mouse fibroblast, human dermal fibroblast, and human skin cancer cells [21, 22]. [score:1]
2. Proposed Mechanism of miR-302/367-Mediated Reprogramming. [score:1]
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[+] score: 67
As both the miR-371–373 and miR-302/367 microRNA clusters are ESC-specific pluripotency markers (Suh et al., 2004; Thomson et al., 2006; Lakshmipathy et al., 2007; Barroso- del Jesus et al., 2008; Laurent, 2008), the co-ordinate expression of microRNAs from these clusters in malignant GCTs either represents the persistence of an embryonic pattern of microRNA expression that is not present in normal, somatically differentiated tissues, or acquired re -expression, regulated by an as yet undetermined mechanism(s) (Palmer et al., 2010). [score:8]
This analysis showed that the identical seed region shared by microRNAs from the over-expressed miR-371–373 and miR-302/367 clusters resulted in a global down-regulation of target mRNAs in malignant GCTs and was therefore of functional significance (Palmer et al., 2010). [score:8]
As for miR-371–373 and miR-302/367 over -expression, let-7 under -expression in malignant GCTs was universal, occurring regardless of patient age, histological subtype or site of disease, thereby extending published reports describing predominantly or exclusively tumours from adult patients (Cao et al., 2011a, b; Gillis et al., 2011; Xue et al., 2011). [score:7]
In summary, miR-371–373 and miR-302/267 cluster over -expression occurs in all malignant GCTs, regardless of patient age, histological subtype and anatomical site of disease (Palmer et al., 2010). [score:5]
In particular, further over -expression of the miR-302/367 cluster was observed in YSTs compared with seminomas, which resulted in the down-regulation of cancer -associated protein-coding genes (Murray et al., 2010). [score:5]
Furthermore, in another study the relative miR-302/367 over -expression in YSTs was associated with increased bone morphogenetic protein (BMP) signalling activity in YSTs (compared with seminomas), presumably via multiple predicted mRNA targets in the transforming growth factor –beta/BMP pathway (Fustino et al., 2011). [score:4]
Figure 2Differential expression of the miR-371–373 and miR-302/367 clusters in malignant germ cell tumours (GCTs) [adapted from (Palmer et al., 2010)]. [score:3]
In contrast, all malignant GCTs over-express the miR-371–373 and miR-302/367 clusters regardless of patient age, histological subtype or anatomical tumour site. [score:3]
control samples were identified, in addition to the key over-expressed miR-371–373 and miR-302/367 clusters (Palmer et al., 2010). [score:3]
However, the most significant finding was that the miR-371–373 and miR-302/367 clusters were over-expressed in all malignant GCTs, independent of patient age (paediatric or adult), tumour histological subtype (YST, seminoma or EC) or anatomical site (gonadal or extragonadal) (Palmer et al., 2010). [score:3]
In addition to the universal miR-371–373 and miR-302/367 over -expression findings, each subtype of malignant GCT was additionally characterized by specific abnormalities of microRNA expression (Palmer et al., 2010). [score:3]
As miR-302/367 expression is lost in cells and tissues showing somatic differentiation (Suh et al., 2004; Barroso- del Jesus et al., 2008), it is likely that levels peak during early extraembryonic differentiation. [score:3]
Importantly for potential clinical use as highly sensitive and specific universal biomarkers of malignant GCTs, the expression levels of the eight main microRNA members from the miR-371–373 and miR-302/367 clusters accurately segregated malignant GCTs from the non-malignant group, comprising fetal and gonadal control samples and benign teratomas (Fig. 2) (Palmer et al., 2010). [score:3]
The first report of serum microRNA expression in malignant GCTs contained a detailed multiplexed qRT-PCR methodology and demonstrated elevated serum levels of all eight main members of the miR-371–373 and miR-302/367 clusters in the serum of a paediatric patient compared with pooled normal serum (Murray et al., 2011). [score:2]
If so, dynamic changes in miR-302/367 levels in normal embryonic development (Stadler et al., 2010) would be mirrored in GCTs showing equivalent differentiation states (Murray et al., 2010). [score:2]
Figure 3MicroRNAs from the miR-371–373 and miR-302/367 clusters as novel serum biomarkers of malignant germ cell tumours (GCTs) [adapted from (Murray & Coleman, 2012)]. [score:1]
Thus, microRNAs of the miR-371–373 and miR-302/367 clusters are emerging as promising bodyfluid biomarkers to improve clinical management of malignant GCTs. [score:1]
Of note, the miR-371–373 cluster is involved in maintaining the pluripotent state in ESCs and germline stem cells, whereas miR-302/367 members are induced during the first stages of differentiation (Zovoilis et al., 2008). [score:1]
Levels of miR-372 from the miR-371–373 cluster (left) and miR-367 from the miR-302/367 cluster (right) in the serum at the time of diagnosis in eight malignant GCTs of different patient age, anatomical site and histological subtype. [score:1]
Hierarchical clustering analysis based on the eight main microRNAs from the miR-371–373 and miR-302/367 clusters (rows) segregates (A) paediatric and (B) adult malignant GCT samples from non-malignant controls (comprising benign teratomas and normal gonadal controls) (columns). [score:1]
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To infer the function of these miRNAs, we predicted 2,436 targets for the miR-302 cluster and 4,691 targets for the miR-520 cluster by querying the public database miRNAMap 2.0, and 2,284 target genes were shared by both clusters suggesting functional similarity. [score:7]
miRNAs expressed at high levels in hES cells and downregulated during differentiation or in adult cells included the well-known miR-302 family, miR-200 family, and miR-372. [score:6]
Among the hES signature miRNAs, the miR-520 cluster shared a similar expression pattern and seed sequence as the well known miR-302 family and targeted the same genes as the miR-302 family. [score:5]
In addition, we identified 12 other hES upregulated miRNAs in this cluster: miR-302a, miR-302b, miR-302c, miR-302d, miR-519b, miR-519c, miR-520a, miR-520b, miR-520c, miR-520d, miR-520e which share a consensus seed sequence: AAGUGC [24]. [score:4]
The upregulation of miR-302 cluster and miR-520 cluster in hES cells suggests their ability to modulate local chromatin states which is necessary for stem cell pluripotency [58, 59]. [score:4]
Among the miRNAs upregulated in hES cells, we observed 7 miRNAs were located in the miR-302 cluster and 21 miRNAs were located in miR-520 cluster. [score:4]
In addition, we identified 21 hES upregulated miRNAs that were co-localized in a cluster on chromosome 19, the miR-520 cluster, many of which shared consensus seed sequence with miR-302 family and which can be used as candidate biomarkers for pluripotency (Additional file 1). [score:4]
Especially, members of the miR-302 cluster on chromosome 4 and miR-520 cluster on chromosome 19 were highly expressed in undifferentiated hES cells. [score:3]
From our data, the expression of miR-302a, miR-302b, miR-302c, miR-302d and miR-367, which are co-located in a cluster on chromosome 4 were highly correlated (R [2 ]= 0.78–0.98). [score:3]
To visualize the functions of these miRNA targeted genes, a binary (red indicate participate in the functional category and green indicate not) heatmap was used to indicate functional commonality among all miRNAs in miR-302 and miR-520 clusters. [score:3]
Gene Ontology (GO) enrichment analysis confirmed that the inferred functions of miRNAs within the miR-302 and miR-520 clusters were overlapping based on their involvement in cell growth, negative regulation of cellular metabolic process, negative regulation of transcription, and small GTPase mediated signal transduction. [score:3]
The 20 miRNAs most highly expressed in hES cells, EB, and adult cells respectively were shown in additional file 1. MiR-302a, miR-302b, miR-302c, miR-302d, miR-367, and miR-200c were increased in hES and have previously been reported to be hES-specific [16, 17]. [score:3]
Along with the reports of miR-302 family on chromosome 4 [16, 17, 19, 25, 26], several groups have reported the expression of members of miR-520 cluster on chromosome 19 in hES cells [24, 26, 29]. [score:3]
The miR-302 cluster and miR-520 cluster target large groups of genes which share overlapping functions based on Gene Ontology (GO) analysis. [score:3]
Signature miRNAs, such as the miR-302 family, the miR-200 family have been reported in human [16, 17] and mouse embryonic stem cells [18- 20]. [score:1]
Figure 8 Sequence and GO analysis of the miR-302 cluster and miR-520 cluster. [score:1]
MiR-520b, miR-302b, miR-302c, miR-302d, miR-519c, miR-520a and miR-302a were clustered closely base on the 48 GO terms analyzed. [score:1]
The members of the miR-302 and miR-520 clusters had similar sequences; they shared a consensus seed sequence: AAGUGC (panel A, seed sequence is highlighted by the purple rectangle). [score:1]
In particular, miR-302a, miR-302b, miR-302c, miR-302d, miR-519b, miR-519c, miR-520a, miR-520b, miR-520c, miR-520d, and miR-520e had a consensus seed sequence: AAGUGC (Figure 8, panel A). [score:1]
Functional comparison of miR-302 cluster and miR-520 cluster. [score:1]
At the Gene Ontology level, miR-520b, miR-302b, miR-302c, miR-302d, miR-519c, miR-520a, and miR-302a formed a cluster (significant GO terms shown as red), and they shared GO terms related to chromatin structure modifications (Panel B). [score:1]
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[+] score: 62
We constructed a bidirectional vector whereby the reporter gene mCherry is conjugated with perfectly complementary miRNA target sites for miR-223, miR-155, miR-142-3p and EGFP expressed in the anti-sense direction conjugated with miR-302a and miR-302d (miR-302 a/d). [score:7]
Expression of the specific miRNAs tested here is unknown within the neural tube-like structures, however there appears to be expression of one or more differentiation specific miRNAs in the interior whereas miR-302a/d appears to be expressed in the surrounding dark pigmented area. [score:7]
Our results show that upon differentiation of the hiPSC into EBs for 25 days, the majority of the cells harboring the reporter vector now expressed EGFP, presumably reflecting the loss of miR-302a/d expression as the cells differentiated. [score:5]
For ectopic expression of miRNAs, we purchased miRNA expressing lentiviral vectors for miR-302a, miR-302b, miR-302c, and miR302-d (PMIRH302abcdPA-2) and miR-155 (PMIRH155PA-1) from System biosciences (Mountain View, CA). [score:5]
Ectopic expression of the miRNAs by co-transduction, either miR-302a, miR-302b, miR-302c, and miR-302d or miR-155 results in ablation of EGFP or mCherry expression, respectively, demonstrating sensitivity of the vector to specific miRNAs (Fig. 1B, miR-302a-d and miR-155, respectively). [score:5]
Among these colonies, approximately 90% were EGFP negative and mCherry positive, indicating expression of miR-302a/d and no expression of the differentiation specific miRNAs, suggesting a partially reprogrammed state for these transformed cells as previously reported [19], [28], [29]. [score:5]
Interestingly, our results show that many cells expressed the hESC-specific miR-302a/d as evidenced by reduction of EGFP expression from day 0 to day 21 (70% to 27%, Fig. 3B), but only a small fraction of those cells actually formed hESC-like colonies. [score:5]
Loss of EGFP expression that is dependent upon expression of the hESC-specific miR-302a/d, is highly specific to the pluripotent cells. [score:5]
Since the fibroblasts do not express miR-302 [19], [20], [21], the cells were positive for EGFP (Fig. 3A, EGFP miR-T and EGFP miR-T/mCherry miR-T). [score:3]
We observed a decrease in EGFP expression over time, presumably reflecting the induction of the hESC specific miRNAs, including miR-302a/d. [score:3]
It is noteworthy that the hESC-specific miRNAs, miR-302a/d, are expressed in a significant proportion of the cells during the process of reprogramming. [score:3]
The miR-302 gene encodes a cluster of eight miRNAs on chromosome 4 (miR-302b*-b-c*-c-a*-a-d-367) that are preferentially expressed in embryonal carcinoma cells, hESCs and hiPSCs [19], [20], [21]. [score:3]
In our studies, more than 50% of the cells express miR-302a/d based upon loss of EGFP during reprogramming at day 14, but only 0.03% of the starting HFF result in hiPSC. [score:3]
These results indicated that miR-302a/d is not sufficient for reprogramming and therefore cannot be used solely as a reporter to identify true hiPSC. [score:1]
Future more selective choice of miRNAs in combination with miR-302 a/d may be utilized to fractionate hiPSC from partially reprogrammed cells based upon their expression profile of fluorescence and to further investigate reprogramming mechanisms. [score:1]
293T cells infected with a lentiviral vector encoding EGFP miR-T/mCherry miR-T were super-infected by a lentiviral vector encoding either miR-302a, miR-302b, miR-302c, and miR-302d (miR-302 a-d) or miR-155 2 days post-infection. [score:1]
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14
[+] score: 61
Because the expression levels of the miR-302 cluster must be precisely regulated, we used different promoters to exogenously express the miR-302 cluster and screened for optimal expression (Additional file 1: Figure S1a, S1b). [score:8]
Therefore, miR-302 s have a positive impact on somatic cell reprogramming, but in some pathways these family members can inhibit reprogramming by indirectly activating targets that inhibit reprogramming. [score:8]
Furthermore, miR-302 family members target the oncogene Bmi1 and suppress tumorigenesis, which can inhibit somatic cell reprogramming [15, 16, 25]. [score:7]
Furthermore, the miR-302 family activates Ink4a and Arf to suppress the tumorigenesis of human pluripotency stem cells by targeting the oncogene Bmi1 [25], and Arf/ p53 pathway activation suppresses somatic cell reprogramming [15, 16]. [score:7]
Therefore, miR-302 s are typically important factors that promote somatic cell reprogramming, but targeted factors that inhibit reprogramming exist in some signal pathways. [score:5]
Lin SL Chang DC Ying SY Leu D Wu DT MicroRNA miR-302 inhibits the tumorigenecity of human pluripotent stem cells by coordinate suppression of the CDK2 and CDK4/6 cell cycle pathwaysCancer Res. [score:5]
Lin SL Chang DC Lin CH Ying SY Leu D Wu DT Regulation of somatic cell reprogramming through inducible mir-302 expressionNucleic Acids Res. [score:4]
In the present study, we constructed a low-risk 6F/BM1-4C reprogramming system, in which we eliminated the tumorigenic factors used in traditional episomal reprogramming systems, such as c-Myc, SV40-LT, and p53 inhibitor, and included Oct4, Glis1, Klf4, Sox2, L-Myc, the miR-302 cluster and four compounds, and then treated cells for no longer than 48 h to efficiently generate iPSCs from hUCs. [score:3]
The miR-302 family, which is specifically expressed in embryonic stem cells (ESCs), can partially or completely replace reprogramming factors and increase reprogramming efficiency [14, 23, 24]. [score:3]
As revealed by AP staining, the combination termed 6F, which includes Oct4, Glis1, Klf4, Sox2, L-Myc, and the miR-302 cluster initially expressed from the CMV promoter (Fig.   1c, d), showed the highest reprogramming efficiency at 19 days post nucleofection. [score:3]
This system includes six low-risk factors (6F), Oct4, Glis1, Klf4, Sox2, L-Myc, and the miR-302 cluster. [score:1]
To induce reprogramming in this study, we employed low-risk factors, including L-Myc, Glis1, lincRNA-ROR, and the miR-302 cluster, and randomly combined them with Oct4, Sox2, and Klf4 in the Epstein–Barr virus-encoded nuclear antigen-1 (EBNA)-oriP episomal vector (Additional file 1: Figure S1a, S1b, S1c). [score:1]
Highlighted are the pluripotency factors Oct4, Sox2, Nanog, and miR-302a. [score:1]
The miR-302 family can increase reprogramming efficiency by replacing reprogramming factors [14, 23, 24]. [score:1]
In this study, we applied hUCs as donor cells to induce iPSCs using low-risk factors, and then we screened a combination of low-risk reprogramming factors, including Oct4, Glis1, Klf4, Sox2, L-Myc, and the miR-302 cluster. [score:1]
The sequence of the miR-302 cluster was cloned from genomic DNA. [score:1]
Highlighted are the pluripotency factors Oct4, Sox2, Nanog, and miR-302a. [score:1]
Three groups of cells harboring the reprogramming factors Oct4, Glis1, Klf4, Sox2, L-Myc, lincRNA-ROR, and the miR-302 cluster with high AP -positive scores were selected for further analysis (Additional file 5: Figure S2c, S2d). [score:1]
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[+] score: 60
For example, mir-302 family was highly expressed in PSC but maintained an upregulated expression at the mesoderm stage, with a significant decrease thereafter. [score:8]
Similarly, isoforms of the miR-302a-5p had several unique targets (242) that were not present in the repertoire of targets regulated by the exact microRNA. [score:6]
This latter observation suggests that despite the overlapping of gene targets, each microRNA from miR-302 cluster interacts with different affinities to their target RNAs. [score:5]
We identified 415 novel predicted targets for the 5′-isomiRs of the miR-302a-3p and 242 for the 5′-isomiRs of the miR-302a-5p, both highly expressed in the PSC. [score:5]
Additionally, we studied the correlation between the target affinity scores of the miR-302 cluster/family for their common targets (see above). [score:5]
mir-302 family members are highly expressed microRNAs in PSC. [score:3]
We chose the miR-302 family, miR-17/92 family and a group of microRNAs highly expressed in CM population. [score:3]
We then analyzed in more detail the microRNA miR-302a and their isomiRs, revealing the case of a particular microRNA being less abundant than its isomiRs and with a repertoire of predicted targets considerably different for most of the discovered 5′-isomiRs. [score:3]
We noticed a higher expression of several isomiRs related to hsa-mir-302a, particularly at the 5′-end. [score:3]
Moreover, the score correlation of predicted targets of the family miR-302 illustrates the synergistic effect that these microRNAs have. [score:3]
The most expressed microRNA family in PSC and MPC was miR-302, which also corresponds to a cluster we named Chr4.1 (first one found in Chormosome 4). [score:3]
Interestingly, for the miR-302a-3p there were several common targets between one of the isomiRs and the microRNA exact sequence, but many were exclusive for each isoform (94 for 302a3p-iso1, 81 for 302a3p-iso2 and 181 for 302a3p-iso3). [score:3]
For both miR-302 and mir-17-92a families many GO terms were related to developmental processes (see complete lists in Supplemental Files  11 and 12). [score:2]
It is necessary to include all family members (as in the case of mir-302) in order to reach significance in the developmental pathways. [score:2]
Many other clusters are composed by a single family (red lines), as for example the mir-302 family/cluster (cluster 4.1). [score:1]
Read counts of each isomiR is presented at the left of the sequence and sequences with a common seed are grouped together and named as iso-1, iso-2 and iso-3 for each miR302a. [score:1]
The well-characterized miR-302 family (mainly represented by miR-302a, miR-302b, miR302c, miR302d) is overwhelmingly expressed in both PSC and MPC. [score:1]
The cluster word clouds complemented the family word clouds; hence, Chr4.1 (where mir-302 family is located) was highly represented in PSC and MPC. [score:1]
In the case of MPC, the most distinctive family apart from the miR-302, is the miR-25 (miR-25, miR-92a and miR-92b). [score:1]
In Fig.   5C the upper part of the matrix shows the correlation scores between pairwise comparisons of miR-302 members. [score:1]
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[+] score: 57
As shown in Figure 4I, expression of miR-302a, miR-302b, miR-302d and miR-367 was identified in p100−/− cells, and such ectopic expression led to a dramatically inhibition of cyclin d1 3′-UTR activity in p100−/− cells (Figure 4J). [score:7]
The previous studies show that miR-302 inhibits the proliferation of endometrial cells with inhibiting CDK1 and Cyclin D1 expression in MCF7 and HepG2 [42, 43]. [score:7]
Transfectants of miR-302/367 cluster construct in UMUC3(shp100) cells also expressed a high level of miR-302d (Figure 4K) and inhibited Cyclin D1 expression in both UNUC3 (shp100) and p100−/− cells (Figure 4L and 4M). [score:7]
To provide evidence demonstrating whether miR-302d, rather than miR-302a/miR-302b, direct binds to cyclin d1 mRNA 3′-UTR and inhibits Cyclin D1 protein translation, putative miR-302d binding site in cyclin d1 3′-UTR reporter was point mutated as indicated in Figure 4O. [score:6]
The expression of these miRNAs were therefore determined in both p100+/+ and p100−/− cells, and the expression of miR-302a, miR-302b and miR-302d was found to be significantly decreased in p100−/− cells, whereas there was no observable difference on miR-17, miR-19a, miR-20a and miR-106b between p100+/+ and p100−/− cells (Figure 4E). [score:5]
Our finding is supported by previous report that miR-302 expression suppresses Cyclin D1-CDK4/6 pathways [42]. [score:5]
The results also showed that ectopic p100 expression in p100−/− cells only restored miR-302d expression (Figure 4F), demonstrating that miR-302d, not miR-302a or miR-302b, was activated by p100. [score:5]
These results demonstrate that miR-302 inhibits cyclin d1 3′-UTR luciferase activity, Cyclin D1 protein expression and cell cycle progression. [score:5]
To determine potential role of miR-302d in the Cyclin D1 expression, a miR-302/367 cluster construct was transfected into p100−/− cells and UMUC3 (shp100) cells, respectively. [score:3]
Construct expressing miR-302/367 was obtained from Addgene (Cambridge, MA). [score:3]
The p100-stimulation of CREB phosphorylation in its activation of LARP7/miR-302 transcription was convincingly demonstrated by using CREB knockdown in p100−/−(p100) cells. [score:2]
Constitutive expression of miR302 in UMUC3(shp100) cells induced G [0]/G [1] growth arrest as compared with UMUC3 (shp100/Vector) cells (Figure 4N). [score:2]
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[+] score: 54
The numerical in brackets shows the ranking of each pathway Table 3 Common validated target genes shared between the C19MC-AAGUGC-miRNAs and the miR-302/-372 families AAGUGC-miRNASeed position [a] Target transcript miR-302/-372 C19MC miR-302c miR-520e I NIK[10, 15] miR-373 miR-520c I MT1-MMP, mTOR, SIRT1[14, 21] miR-372, -373 miR-520c, -520e I RelA[12] miR-302b, -372, -373 miR-520c, -520e I TGFβR2[9, 12] miR-520b, -520e I CD46[16] miR-302c miR-520c I MICA, MICB, ULBP2[17] miR-519a I RBL2[13] miR-512 IIa miR-519d, -520g IIb SMAD7[19, 20] miR-520g, -520h IIb DAPK2[18, 22] miR-302d, -372 miR-520b, -519b-3p, -520a-3p I CDKN1A[5, 6] miR-519e IIa miR-519d, -520h IIb [a]Group I seed position is the canonical nts 2-7; IIa is nts 1-6 and IIb is other non-canonical position, as defined in Fig.   2a The 2058 putative target genes were further subjected to GO analysis and KEGG pathway annotation (Fig.   3b-d). [score:7]
The numerical in brackets shows the ranking of each pathway Table 3 Common validated target genes shared between the C19MC-AAGUGC-miRNAs and the miR-302/-372 families AAGUGC-miRNASeed position [a] Target transcript miR-302/-372 C19MC miR-302c miR-520e I NIK[10, 15] miR-373 miR-520c I MT1-MMP, mTOR, SIRT1[14, 21] miR-372, -373 miR-520c, -520e I RelA[12] miR-302b, -372, -373 miR-520c, -520e I TGFβR2[9, 12] miR-520b, -520e I CD46[16] miR-302c miR-520c I MICA, MICB, ULBP2[17] miR-519a I RBL2[13] miR-512 IIa miR-519d, -520g IIb SMAD7[19, 20] miR-520g, -520h IIb DAPK2[18, 22] miR-302d, -372 miR-520b, -519b-3p, -520a-3p I CDKN1A[5, 6] miR-519e IIa miR-519d, -520h IIb [a]Group I seed position is the canonical nts 2-7; IIa is nts 1-6 and IIb is other non-canonical position, as defined in Fig.   2a The 2058 putative target genes were further subjected to GO analysis and KEGG pathway annotation (Fig.   3b-d). [score:7]
Similarly, eight miR-302-like C19MC miRNAs were previously shown to promote cell proliferation and cell-cycle progression by targeting p21, an inhibitor of the G1/S transition, as for the miR-302 and -372 families [5, 6]. [score:5]
Echoing these findings, the miR-302-like C19MC are also predominantly 3p-biased, possibly targeting genes which are biologically significant in regulating the stemness of stem cells and the tumor phenotype in cancers. [score:4]
Hence, despite the presence of the AAGUGC seed sequence, it is more likely that the nts 2-7 canonical subgroup of the C19MC-AAGUGC-miRNAs may target genes that share similar functions as the miR-302/-372 miRNAs. [score:3]
MiR-302 -driven cellular reprogramming coordinates stem cell division by regulating targets in the cell cycle, particularly at the G1/S restriction point [5]. [score:3]
Consistent with the bioinformatics prediction, a literature review showed that a number of validated targets have indeed been reported to be shared between the miR-302/372 and the group I C19MC-AAGCGU-miRNA families (Table  3). [score:3]
Many of the expressed miRNAs share the “AAGUGC” seed sequence of the known reprogramming miR-302 miRNA family; these miRNAs are called the C19MC-AAGUGC-miRNAs in this work (see Fig.   2a and depiction below). [score:3]
However, the miR-520 and miR-302/372 families share a significant number of target genes (Fig.   3a) suggesting common biological functions. [score:3]
a Venn diagrams of predicted target genes of the miR-302/372 families and group I of the C19MC-AAGUGC-miRNAs. [score:3]
The group I miR-519 subfamily also shares 262 putative target genes with the miR-302/-372 families, far fewer than the miR-520 subfamily (Fig.   3a, red box). [score:3]
In particular, C19MC-AAGUGC-miRNAs with the nucleotides 2-7 canonical seed position as in miR-302/-372 miRNAs, may play similar roles as miR-302/-372 in induced pluripotency. [score:1]
The results showed that 1185 putative shared genes were obtained between the miR-520 and -302/372 families (Fig.   3a, blue box and Additional file 1: Table S1), suggesting that the miR-520 subfamily might share similar biological functions with the miR-302/372 family. [score:1]
Bioinformatics analysis showed that sixteen of the C19MC miRNAs share the same “AAGUGC” seed sequence with members of the miR-302/-372 family, which are known cellular reprogramming factors. [score:1]
Moreover, the identification of sixteen miR-302-like C19MC miRNAs predicts functions in promoting “stemness” as the miR-302 and miR-372 families. [score:1]
More specifically, a subgroup of sixteen C19MC miRNAs has been identified that shares the same AAGUGC seed sequence as the reprogramming miR-302/372 family, predicting contribution of the C19MC-AAGUGC-miRNAs to the reprograming process. [score:1]
Further elucidation of the biological functions of C19MC miRNAs, particular the miRNA-302-like subclass, may lead to potential applications in more efficient cellular reprogramming and in cancer therapy. [score:1]
On sequence alignment, sixteen C19MC miRNAs were found to share the same seed sequence, 5’-AAGUGC-3’, with the reported reprogramming-able miR-302 and miR-372 miRNA families [8, 9] (Fig.   2a). [score:1]
Possible biological functions of a subset of miR-302-like C19MC miRNAs, were further investigated by bioinformatics analysis, which predicted targeting at the apoptosis pathway in the tumorigenesis of cancer cells and induced pluripotency in stem cells. [score:1]
Furthermore, it is noted that the AAGUGC seed position at 5’ end is variable among the C19MC-AAGUGC-miRNAs: subgroup I miRNAs, which includes eight miR-519 and -520 subfamilies, have the seed sequence located at the canonical and optimal 5’-nucleotide positions (nts) 2-7, as in the miR-302/-372 families; the seed sequence of the four subgroup IIa miRNAs is at location nts 1-6, and that of the remaining subgroup IIb miRNAs is at nts 3-8 and 4-9 (Fig.   2a). [score:1]
a The sixteen C19MC miRNAs that share the AAGUGC hexameric seed sequence (in bold letters and boxed in red) with the miR-302 (in blue letters) and miR-372 (in green letters) families are shown. [score:1]
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[+] score: 46
Consistently, the major mature miRNA product, miR-302a, was expressed strongly in undifferentiated NT2 cells, and down-regulated during the RA time-course while its minor counterpart, miR-302a*, was not expressed in any cell type. [score:8]
Five of the other 6 miRNAs were members of a known ES cell specific polycistronic cluster (miR-367, miR-302a, b, c and d) and one, miR-150, which was only weakly expressed in NT2-undiff, were down-regulated during the RA time-course (Fig. 4). [score:6]
Additionally, three minor products of the polycistronic cluster (miR-302a*, miR-302b* and miR-302c*) were also down-regulated, but were expressed below threshold in NT2-undiff (Fig. 3B). [score:6]
Moreover, the undifferentiated NT2 cells expressed members of ES cell specific polycistronic cluster (miR-367, miR-302a, b, c and d), consistent with their stem cell properties [29], [30], which, subsequently, were down-regulated as the RA time-course progressed and the cells acquired the neural precursor features [32], [34]. [score:6]
Additionally, 2 known miRNAs, miR-10a and miR-302a, were used as controls for up- and down-regulation, respectively. [score:4]
For example, the polycistronic miR-302 cluster was found to be strongly down-regulated during the RA time-course (Fig. 3A). [score:4]
In another study, Hohjoh and Fukushima [27] examine the profiles of 180 mouse and 127 human miRNAs in human (NTera2/D1) and rodent (P19D and Neuro2a) cell lines, and report that the ES cell specific miR-302 cluster [29], [30] and miR-124a [10] show the opposite patterns of expression in response to the RA treatment and may mark the onset of neural differentiation. [score:3]
The expression levels of the same miR-302 (A; bottom left panel) and miR-181 (B; bottom right panel) genomic clusters in NT2-undiff and NT2-28D plotted as yellow and blue bars, respectively. [score:3]
0011109.g003 Figure 3The expression levels of the members of the miR-302 (A) and the miR-181 (B) genomic clusters during neural differentiation plotted as heatmaps (top panels). [score:3]
The expression levels of the members of the miR-302 (A) and the miR-181 (B) genomic clusters during neural differentiation plotted as heatmaps (top panels). [score:3]
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[+] score: 42
They claimed that miR-302 is a human tumor suppressor capable of attenuating rapid cell growth, causing tumor cell apoptosis, and inhibiting tumor cell invasion and metastasis. [score:5]
The cluster of miR-302 is the most abundantly expressed miRNA in undifferentiated ESCs, and its expression is sharply turned off upon the induction of differentiation (20). [score:5]
In NT2 and other pluripotent cells, high expression of miR-302 coincides with high expression of OCT4. [score:5]
Members of the miR-302 cluster are highly expressed in embryonic stem cells (ESC), where they regulate cell self-renewal and pluripotency. [score:4]
Interestingly, the ESC-specific transcription factors OCT4, SOX2, Nanog and Rex-1 have binding sites on the miR-302 promoter, thus regulating its expression (20, 21). [score:4]
The expression pattern of miR-302 and ips genes greatly varies among the diffuse versus intestinal, subgroups of tumors (Unpublished data). [score:3]
However, this finding was in agreement with a recent report by Lin et al. (38) who have demonstrated tumor suppressive activity for miR-302. [score:3]
Then, miR-302 expression in tumor samples was normalized to the matched non-tumor samples (2 [‸‑ΔΔCT]). [score:3]
There is a positive correlation between miR-302 expression and induced pluripotency (ips) genes, including OCT4 variants, in gastric adenocarcinoma. [score:3]
However, more work is needed on the expression and function of miR-302 on different tumor types before we can assign a general role for miR-302 in tumor initiation and progression. [score:3]
Thus, our findings have supported Lin's results and provided an important insight into the role of miR-302 in tumorigenesis. [score:1]
The miR-302 promoter has binding sites for the main ESC-specific transcription factors, i. e. OCT4, Nanog, Sox2, and Rex1 (20, 21). [score:1]
Members of the miR-302 cluster (miR-302a, miR-302b, miR-302c, miR-302d, and miR-367) are the most abundant miRNAs in human embryonic stem cells (hESCs). [score:1]
The sample size was calculated based on the assumption of 1 ΔCT difference for miR-302 expression between tumor and non-tumor gastric samples, with the consideration of a type I error of 0.05 and type II error of 0.20. [score:1]
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[+] score: 41
Several mRNAs targeted by neuronal miRNAs (i. e., miR-124, miR-9 and miR-96) were downregulated upon increased miRNA expression, consistent with expectations of the role of miRNAs in repressing downstream targets, whereas for the decreasing miR-302 cluster and miR-103, similar proportions of the targets were up- and downregulated. [score:15]
We initially analyzed the correlations between the expression levels of miR-302a-d, miR-124, miR-96, miR-9 and miR-103 and their experimentally validated (miRTarBase 4.4 (Hsu et al, 2011)) mRNA targets that are expressed in iNGN cells (Supplementary Fig S8). [score:7]
This cluster is transcriptionally regulated by NANOG, POU5F1 and SOX2 (Barroso- del Jesus et al, 2009), and since these pluripotency factors were downregulated, the decrease in the miR-302/367 cluster levels was expected. [score:5]
To further test the impact of miRNAs in iNGN cell differentiation, we knocked down the expression of the miR-302/367 cluster and miR-124 in iNGN cells by miRNA sponges (Ebert et al, 2007). [score:4]
At every time point and for each replicate, the relative miR-124 qRT–PCR expression levels were normalized to miR-302a and miR-96 and these ratios were multiplied with corresponding nCounter counts for miR-302a and miR-96 separately. [score:3]
Validated miRNA targets (Hsu et al, 2011) were used for correlation analysis with miR-302a-d, miR-9, miR-96, and miR-103. [score:3]
At day 0, the uninduced iNGN samples had miRNA signatures of stem cells; the miR-302/367 cluster dominated their profile (50.3% of the total amount of miRNAs) (Fig 4A; Supplementary Fig S5) consistent with previous studies that demonstrated its role in regulating self-renewal and preserving pluripotency (Lipchina et al, 2012; Wang et al, 2013). [score:2]
Thus, miRNA profiles rapidly changed in the course of iNGN differentiation, consistent with the loss of pluripotency (miR-302 cluster) and the establishment of neuronal miRNA signatures (miR-124, miR-96 and miR-9). [score:1]
The miRNA sponge sequences for hsa-miR-124 and the hsa-miR-302/367 cluster were in silico designed as previously described by Krol et al (2010), synthesized (Genewiz), PCR-amplified and placed downstream of a GFP-T2A-puromycin cassette driven by the EF1α promoter within a lentiviral vector (Addgene Plasmid 12252: pRRLSIN. [score:1]
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21
[+] score: 35
[d] As annotated by miRBase Table 2 Top 10 differentially expressed piRNA clusters (A), tRNA-derived small RNAs (B) and miRNAs (C) A Top 10 differentially expressed small RNA clusters Genomic location Log2 Fold Change P-value (adjusted) 1 chr15:21,925,462-21,930,432 −6.18 2.62e-29 2 chr15:20,841,664-20,850,393 −6.18 1.31e-27 3 chr15:23,510,099-23,514,854 −6.25 1.46e-26 4 chr18:55,837,529-55,838,674 inf 1.46e-26 5 chr1:2,237,998-2,241,778 −6.55 1.88e-26 6 chr15:23,386,898-23,404,357 −6.06 1.88e-26 7 chr15:28,583,287-28,593,483 −5.93 1.88e-26 8 chr4:190,801,280-190,804,618 −6.21 1.88e-26 9 chr15:20,723,798-20,737,584 −5.94 3.27e-26 10 chr18:14,460,205-14,464,963 −6.30 3.27e-26 B Differentially expressed tRNA-derived small RNAs tRNA Log2 Fold Change P-value (adjusted) 1 Glu-GAG (chr13, 45492060) −2.51 2.50e-09 2 Glu-GAG (chr15, 26327380) −2.63 2.50e-09 3 Asp-GAY (chrX, 18996334) −3.49 3.40e-06 C Top 10 differentially expressed miRNAs miRNA Log2 Fold Change p-value (adjusted) 1 hsa-miR-302d-3p 11.25 1.95e-13 2 hsa-miR-302a-3p 11.65 1.23e-12 3 hsa-mir-302b 11.38 1.23e-12 4 hsa-mir-302a 11. [score:9]
A study using a genetic screen of primary human cells supported this observation and found that both miR-372-373 and miR-302 may act as TGCT oncogenes through inhibition of target genes such as Large Tumor Suppressor homolog 2 (LATS2) [12]. [score:7]
Expression analysis of miRNAs shows upregulation of the miR-302 and miR-371-373 clusters. [score:6]
The most significantly differentially expressed miRNAs were from the miRNA clusters miR-302/367 and miR-371-373, seen in the upper right corner of the plot. [score:3]
We have confirmed the association between TGCT and high expression of the miR-371-373 and miR-302/367 clusters. [score:3]
Moreover, the miRNAs miR-371-373, miR-302 and miR-146 have previously been shown to display a TGCT-specific expression pattern [12– 14], indicating a potential role for miRNAs in TGCT pathogenesis. [score:3]
Our results confirm previous findings indicating that miRNAs have a relevant role during testicular carcinogenesis, since overexpression of the miR-371-373, miR-302 and miR-367-3p clusters was noted in malignant TGCT tissue [12, 57, 58]. [score:3]
Several of the miR-371-373 and miR-302/367 cluster members have shown a sufficiently strong association with TGCT [12, 57, 58] to serve as biomarkers of TGCT. [score:1]
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22
[+] score: 33
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-18a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-21, hsa-mir-23a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-96, hsa-mir-98, hsa-mir-99a, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-99a, mmu-mir-127, mmu-mir-128-1, mmu-mir-136, mmu-mir-142a, mmu-mir-145a, mmu-mir-10b, mmu-mir-182, mmu-mir-183, mmu-mir-187, mmu-mir-193a, mmu-mir-195a, mmu-mir-200b, mmu-mir-206, mmu-mir-143, hsa-mir-139, hsa-mir-10b, hsa-mir-182, hsa-mir-183, hsa-mir-187, hsa-mir-210, hsa-mir-216a, hsa-mir-217, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-224, hsa-mir-200b, mmu-mir-302a, mmu-let-7d, mmu-mir-106a, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-128-1, hsa-mir-142, hsa-mir-143, hsa-mir-145, hsa-mir-127, hsa-mir-136, hsa-mir-193a, hsa-mir-195, hsa-mir-206, mmu-mir-19b-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-18a, mmu-mir-21a, mmu-mir-23a, mmu-mir-31, mmu-mir-92a-2, mmu-mir-96, mmu-mir-98, hsa-mir-200c, mmu-mir-17, mmu-mir-139, mmu-mir-200c, mmu-mir-210, mmu-mir-216a, mmu-mir-219a-1, mmu-mir-221, mmu-mir-222, mmu-mir-224, mmu-mir-19b-1, mmu-mir-92a-1, mmu-mir-128-2, hsa-mir-128-2, mmu-mir-217, hsa-mir-200a, hsa-mir-219a-2, mmu-mir-219a-2, hsa-mir-363, mmu-mir-363, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-371a, hsa-mir-18b, hsa-mir-20b, hsa-mir-452, mmu-mir-452, ssc-mir-106a, ssc-mir-145, ssc-mir-216-1, ssc-mir-217-1, ssc-mir-224, ssc-mir-23a, ssc-mir-183, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-128-1, ssc-mir-136, ssc-mir-139, ssc-mir-18a, ssc-mir-21, hsa-mir-146b, hsa-mir-493, hsa-mir-495, hsa-mir-497, hsa-mir-505, mmu-mir-20b, hsa-mir-92b, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, hsa-mir-671, mmu-mir-216b, mmu-mir-671, mmu-mir-497a, mmu-mir-495, mmu-mir-146b, mmu-mir-708, mmu-mir-505, mmu-mir-18b, mmu-mir-493, mmu-mir-92b, hsa-mir-708, hsa-mir-216b, hsa-mir-935, hsa-mir-302e, hsa-mir-302f, ssc-mir-17, ssc-mir-210, ssc-mir-221, mmu-mir-1839, ssc-mir-146b, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-128-2, ssc-mir-143, ssc-mir-10b, ssc-mir-23b, ssc-mir-193a, ssc-mir-99a, ssc-mir-98, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-142, ssc-mir-497, ssc-mir-195, ssc-mir-127, ssc-mir-222, ssc-mir-708, ssc-mir-935, ssc-mir-19b-2, ssc-mir-19b-1, ssc-mir-1839, ssc-mir-505, ssc-mir-363-1, hsa-mir-219b, hsa-mir-371b, ssc-let-7a-2, ssc-mir-18b, ssc-mir-187, ssc-mir-218b, ssc-mir-219a, mmu-mir-195b, mmu-mir-145b, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-31, ssc-mir-182, ssc-mir-216-2, ssc-mir-217-2, ssc-mir-363-2, ssc-mir-452, ssc-mir-493, ssc-mir-671, mmu-let-7k, ssc-mir-7138, mmu-mir-219b, mmu-mir-216c, mmu-mir-142b, mmu-mir-497b, mmu-mir-935, ssc-mir-9843, ssc-mir-371, ssc-mir-219b, ssc-mir-96, ssc-mir-200b
In our study, the putative porcine miR-302 cluster was highly expressed in both types of piPSCs, but hpiPSCs had a much higher expression level that was similar to that in EpiSCs. [score:5]
Regulation of somatic cell reprogramming through inducible mir-302 expression. [score:4]
This study demonstrated that overexpression of the whole porcine miR-106a-363 cluster and miR-302 cluster combined with OSKM improved the efficiency of piPSCs induction. [score:3]
Of note, the piPSCs highly expressing the miR-106a-363 cluster and putative porcine miR-302 cluster had enhanced reprogramming efficiency. [score:3]
This indicated that hpiPSCs dominantly expressed the miR-302 cluster. [score:3]
The miR-302 cluster regulates the PI3K-Akt signaling pathway, MAPK signaling pathway, TGF-β signaling pathway, lysine degradation and other processes (Fig 7B). [score:2]
It was previously reported that the miR-106a-363 cluster and miR-302 cluster promote reprogramming in mice by negatively regulating the TGF-β signaling pathway [35, 36]. [score:2]
The miR-106a-363 cluster and miR-302 cluster were both highly expressed in piPSCs compared with pEFs. [score:2]
Genomic location of the putative porcine miR-302 cluster. [score:1]
A putative porcine miR-302 cluster was predicted by miRdeep2. [score:1]
We observed that the number of AP -positive colonies was highest in the cells transfected with the putative miR-302 cluster group, then in those with the miR-106a-363 cluster group then in the control group (Fig 7E and Fig 7F). [score:1]
These findings indicate that the miR-106a-363 cluster and putative miR-302 cluster exhibited an improved reprogramming efficiency, which was consistent with the results observed in mouse cells. [score:1]
Transfection of has-miR-302a and has-miR-302b miRNA mimics effectively enhanced the porcine reprogramming efficiency and reduced the induction time for piPSCs in the OSKM and OSK induction systems in a previous study [58]. [score:1]
The miR-106a-363 cluster and putative miR-302 cluster therefore appear to have conserved functions in promoting the reprogramming efficiency in porcine cells. [score:1]
An approximately 1.5 kb range harboring the whole miRNA-106a-363 cluster and a 2 kb range containing the putative porcine miR-302 cluster were amplified. [score:1]
The pMXs system separately carrying the porcine miR-106a-363 cluster and putative porcine miR-302 cluster were used to reprogram the pEFs in combination with pMXs-Oct4, pMXs-Sox2, pMXs-Klf4 and pMXs-Myc (OSKM, laboratory stored). [score:1]
Of note, we found three novel miRNAs, ssc_38501, ssc_38503 and ssc_38508, which were very similar to has-miR-302a and has-miR-302b in terms of their sequences (Fig 6C). [score:1]
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[+] score: 33
The expression of gene NR2F2 is increased during differentiation when the expression of OCT4 gene and miR-302 declines (Rosa and Brivanlou, 2011). [score:5]
Several miRNA, especially miR-302 and miR-372 have been directly involved in enhancing of HFF reprogramming (Subramanyam et al., 2011) revealing the possibility to directly target these miRNAs to reprogram the HFF without Yamanaka factors. [score:5]
Type of cells Processes involved Non-coding RNA Reference hESC Pluripotency, self-renewal, cell cycle and fate specification miR-302 Suh et al. (2004), Bar et al. (2008), Lipchina et al. (2011) hESC Inhibition of pluripotency miR-145 Xu et al. (2009) iPSC Pluripotency miR-17, miR-106b, and miR-106a Li et al. (2011) Fibroblasts to iPSC Reprogramming miR-302, miR-372 Anokye-Danso et al. (2011), 2012, Subramanyam et al. (2011) Fibroblasts to iPSC Reprogramming Combination of miR-302, miR-200c, and miR-369 Miyoshi et al. (2011) iPSC Reprogramming LincRNAs Loewer et al. (2010) hESC Neural differentiation LincRNAs Ng et al. (2012) iPS-derived neural progenitors Neural differentiation LincRNAs Lin et al. (2011) hESC Differentiation to neuroectoderm miR-200, miR-96 Du et al. (2013) hESC-derived neural stem cells Suppression of selfrenewal, neural differentiation miR-124, miR-125b and miR-9/9 Roese-Koerner et al. (2013) hESC Neural differentiation miR7 Liu et al. (2012) hESC Neural differentiation miR125 Boissart et al. (2012) hESC, human embryonic stem cells; iPSC, induced pluripotent stem cells. [score:5]
Rosa and Brivanlou (2011) have shown that Oct4 and miR-302 inhibit NR2F2, which in turn inhibits Oct4. [score:5]
A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. [score:3]
Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP response. [score:3]
Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells. [score:3]
This study showed important biological function of mir-302 and NR2F2 in human early development and cell fate determination. [score:2]
Another study confirmed that fully pluripotent stem cells can be obtained by introducing other miRNA such as combination of miR-302, miR-200c, and miR-369 (Miyoshi et al., 2011). [score:1]
The most abundant miRNA transcript in hESCs is mir-302 which encodes for miR-302a/b/c/d and mir-367 (Suh et al., 2004) and is under the control of Oct4, Sox2, and Nanog. [score:1]
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[+] score: 31
miRNA-302/367 promotes proliferation and accelerates G1 to S transition of the cell cycle by the targeting the Rb family and CDK1NA [13], whereas the miR-17/92 cluster enhances reprogramming efficiency by downregulating PTEN, a renowned tumor suppressor [11]. [score:8]
Similarly, miR-302/367 and miR-200 play a crucial role in iPSC generation by targeting EMT-related genes TGFβR2 and ZEB1/ZEB2, respectively [12, 14], echoing our finding of miR-524-5p regulation of ZEB2. [score:4]
Lin SL Chang DC Lin CH Ying SY Leu D Regulation of somatic cell reprogramming through inducible mir-302 expressionNucleic Acids Res. [score:4]
The miR-302/367 cluster enhances reprogramming efficiency by increasing cell division rate [13] and suppressing apoptosis [39], as well as promoting epigenetic reactivation of pluripotency genes [40], as shown here for miR-524-5p. [score:3]
Subramanyam D Lamouille S Judson RL Liu JY Bucay N Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cellsNat Biotechnol. [score:3]
Alternatively, when co-expressed with the OSKM factors, members of the miR-302/367, miR-17/92, and miR-200 clusters are able to enhance reprogramming efficiency [10– 12]. [score:3]
Moreover, both miR-302/367 and miR-200 clusters increase the kinetics of MET during reprogramming through blocking the epithelial-to-mesenchymal transition (EMT)-related genes TGFβR2 and ZEB1/ZEB2 [12, 14]. [score:1]
Other C19MC miRNAs, particularly those that share the same seed sequence with the known reprogramming miR-302/-372 families [7], may also be shown to contribute to cellular reprogramming in future studies. [score:1]
The miR-302/367 cluster miRNAs are able to generate iPSCs [8, 9]. [score:1]
These include the miR-302/367, miR-17/92, and miR-200 clusters, and the chromosome 19 miRNA cluster (C19MC). [score:1]
Others have reported that introduction of multiple members of the miR-302/367 family was able to rapidly and efficiently reprogram fibroblasts into iPSCs with or without other reprogramming factors [9, 10]. [score:1]
Zhang Z Hong Y Xiang D Zhu P Wu E MicroRNA-302/367 cluster governs hESC self-renewal by dually regulating cell cycle and apoptosis pathwaysStem Cell Rep. [score:1]
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[+] score: 30
Using miRNA microarray analysis, qRT-PCR, and bioinformatics, we identified and selected four downregulated miRNAs including hsa-miR-302a-3p and 27 upregulated miRNAs including hsa-miR-30a-5p, hsa-miR-23a-3p, hsa-miR-195a-5p, hsa-miR-99b-5p and hsa-let-7c-5p under these two conditions as having the potential to target genes involved in the regulation of autophagy (Table 2). [score:10]
Moreover, predicted target genes included autophagy core genes; hsa-miR-302a-3p targets ULK1 and hsa-miR-548ah-5p targets ATG16L1, suggesting that these four miRNAs participate in the autophagy process in 5-FU treatment and have the potential to be used to manipulate autophagy in 5-FU based chemotherapy in CRC. [score:7]
There had 4 down-regulated miRNAs including hsa-miR-302a-3p and hsa-miR-548ah-5p; and 27 up-regulated miRNAs including hsa-let-7c-5p and hsa-miR-30a-5p upon 5-FU treatment and starvation in human colon cancer cells. [score:7]
Three kinds of human colon cancer cell lines, HT29 (A), DLD1 (B) and HCT116 (C), were treated as described in Fig. 2. qRT-PCR was performed to validate the alteration of the expression of hsa-miR-302a-3p, hsa-miR-548ah-5p, hsa-miR-30a-5p, hsa-miR-23-3p, hsa-miR-195a-5p and hsa-let-7c-5p under 5-FU treatment (5-FU) and starvation. [score:3]
0114779.g003 Figure 3Three kinds of human colon cancer cell lines, HT29 (A), DLD1 (B) and HCT116 (C), were treated as described in Fig. 2. qRT-PCR was performed to validate the alteration of the expression of hsa-miR-302a-3p, hsa-miR-548ah-5p, hsa-miR-30a-5p, hsa-miR-23-3p, hsa-miR-195a-5p and hsa-let-7c-5p under 5-FU treatment (5-FU) and starvation. [score:3]
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26
[+] score: 29
In differentiated hNSCs, downregulated hsa-miR-96 correlated with SOX5 upregulation of gene and protein expression; similar results were obtained for hsa-miR-302a, hsa-miR-182, hsa-miR-7, hsa-miR-20a/b, and hsa-miR-17 and their target NR4A3. [score:11]
NR4A3, a target of the hsa-miR-7, hsa-miR302a, and miR-17 family, is a member of the NR4A family of nuclear receptors, which, depending on their level of expression, are involved in the differentiation, survival, apoptosis, and regulation of hippocampal axon guidance [50]. [score:6]
Hsa-miR-302a, cluster miR-17-92, and miR-96-182, from 1W, and miR-15 family from 3W were respectively significantly downregulated (Figure  3, Table  1). [score:4]
MiRNA profile of 2D versus 3D was very similar in terms of miRNA types; but the degree and timing of miRNA differential regulation was significantly different for miRNAs involved in both maintenance of stemness (hsa-miR-302a), and cell proliferation (the clusters miR-17-92 and miR-96-183). [score:2]
Top-ranked downregulated miRNAs: hsa-miR-96, hsa-miR-182, hsa-miR-183, hsa-miR-7 and hsa-miR-302a were analyzed by using the DIANA-microT 4.0 algorithm to investigate the KEGG pathway. [score:2]
Significant differences comparing 2D and 3D substrate types were observed at both time points in miRNAs belonging to cluster miR-17-92 [46] and miR-96-182 [47] (regulators of cell proliferation), and hsa-miR-302a (maintenance of stemness), (Figure  3, Table  1). [score:2]
However, in 3D samples, the degree and timing of regulation were significantly different in miRNA members of cluster mi-R17 and miR-96-182, and hsa-miR-302a. [score:2]
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27
[+] score: 28
Transfection of miR302 into cancer cell lines suppresses the expression of genes important in development and differentiation and appears to re-program differentiated cancer cells into a pluripotent ES-like state [45]. [score:6]
The miR302 family targets numerous genes important in early human embryogenesis [45, 46], and is co-expressed with other ES cell genes such as Oct3/4, Sox2 and Nanog. [score:5]
Via inhibition of cyclin D1/D2, CDK2, and BMI-1 expression, miR302 blocks G1-S cell cycle transition, leading to a low proliferation rate [47]. [score:5]
Lin S. L. Chang D. C. Ying S. Y. Leu D. Wu D. T. MicroRNA miR302 inhibits the tumorigenecity of human pluripotent stem cells by coordinate suppression of the CDK2 and CDK4/6 cell cycle pathwaysCancer Res. [score:5]
Decreased levels of Oct4 and miR302 permit NR2F2 expression. [score:3]
miR302 expression is induced by Oct4; together, Oct4 and miR302 silence the transcription factor NR2F2. [score:3]
Fareh M. Turchi L. Virolle V. Debruyne D. Almairac F. de-la-Forest Divonne S. Paquis P. Preynat-Seauve O. Krause K. H. Chneiweiss H. The miR302–367 cluster drastically affects self-renewal and infiltration properties of glioma-initiating cells through CXCR4 repression and consequent disruption of the SHH-GLI-NANOG networkCell Death Differ. [score:1]
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28
[+] score: 28
The expression of pluripotence -associated hsa-miR-302 family was silenced and the expression of Hox miRNA hsa-miR-10 family that regulates gene expression predominantly in neuroectoderm was induced to high levels in these hESC-derived neuronal progenitors hESC-I hNuPs [6, 34]. [score:8]
The drastic expression increase of hsa-miR-10 upon exposure of hESCs to RA suggested that RA might induce the expression of Hox genes and co -expression of Hox miRNA hsa-miR-10 to silence pluripotence -associated genes and miRNA hsa-miR-302 to drive a neuroectoderm fate switch of pluripotent hESCs [6, 34]. [score:7]
Genome-scale profiling of miRNA differential expression patterns during hESC neuronal lineage-specific progression further identified novel sets of stage-specific human embryonic neurogenic miRNAs, including silencing of the prominent pluripotence -associated hsa-miR-302 family and drastic expression increases of Hox hsa-miR-10 and the let-7 miRNAs [6, 34]. [score:5]
Nuclear translocation of NAD (nicotinamide adenine dinucleotide) -dependent histone deacetylase SIRT1 and global chromatin silencing lead to hESC cardiac fate determination, while silencing of pluripotence -associated hsa-miR-302 family and drastic up-regulation of neuroectodermal Hox miRNA hsa-miR-10 family lead to hESC neural fate determination [6]. [score:4]
Nuclear translocation of NAD -dependent histone deacetylase SIRT1 and global chromatin silencing lead to hESC cardiac fate determination, while silencing of pluripotence -associated hsa-miR-302 family and drastic up-regulation of neuroectodermal Hox miRNA hsa-miR-10 family lead to hESC neural fate determination. [score:4]
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Other miRNAs from this paper: hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-302e, hsa-mir-302f
To confirm the direct targeting of the 3′ UTR region of ALDH5A1 mRNA by miR-302, we employed TG1-miR and TG1 cells expressing a Renilla luciferase expression construct containing the ALDH5A1-3′UTR. [score:8]
The miR-302 can be another relevant epigenetic regulator, since it targets ALDH5A1 mRNA, is upregulated in GBM stem-like cells by extra-cellular pro-differentiation cues [15], and is enriched in non-proliferative/differentiated GBM territories (F. Burel-Vandenbos, T. Virolle, unpublished results). [score:7]
Deletion of miR-302 putative target sequence in the 3′UTR of ALDH5A1 mRNA (ALDH5A1-3′UTR-DEL) prevented the binding of the miR, and rescued luciferase activity (Fig.   1e). [score:3]
The cells were chocked twice (at day 0 and day 3) and collected at day 6. Cells were transfected with Renilla Luciferase mRNA and Firefly luciferase mRNA containing either the wild-type form of ALDH5A1-3′UTR or a mutant form of ALDH5A1-3′UTR with a deletion of the miR-302 putative target sequence (ALDH5A1-3′UTR-DEL). [score:3]
Deletion of miR-302 putative target sequence in the 3′UTR of ALDH5A1 mRNA (ALDH5A1-3′UTR-DEL) prevents the binding of the miR, and rescues luciferase activity. [score:3]
e Targeting of the ALDH5A1 transcript by miR-302. [score:3]
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30
[+] score: 27
According to the online database for miRNA target prediction and functional annotations, miRDB [61, 62] the number of predicted targets for miRNAs in the miR-371–373 and miR-302 families range from 469 to 529; interestingly, LATS2 is one of the highest ranking targets on the list for all miR-302 family members as well as miR-372 and miR-373 (Target Score > 98) [62]. [score:9]
These studies have reported higher expression of miRNAs in the miR-371–73 and the miR-302 clusters and lower expression of let-7 in Type I and Type II GCTs compared to normal samples [29– 37]. [score:4]
Specifically, the miR-302 cluster regulates the Nodal inhibitors LEFTY1 and LEFTY2 [64]. [score:4]
We also observed differences in, with miR-371-5p, miR-122, miR-302a, miR-302d, and miR-373 showing elevated expression in one or more histologic subtypes. [score:3]
Using the MIC, we identified correlations across the data features, including six major hubs with higher expression in YST (LEFTY1, LEFTY2, miR302b, miR302a, miR 126, and miR 122) compared with other GCT. [score:2]
Our findings are consistent with previous studies of Type I and Type II GCTs that have identified an overexpression of the miR-371–73 and miR-302 clusters in GCTs compared with normal samples [29– 35]. [score:2]
The miRNA-302 and miRNA-371–373 clusters are highly plausible candidate miRNAs in GCTs given their roles as regulators of embryonic stem cell pluripotency markers [59, 60] which has been discussed extensively in previous studies of GCTs [33, 35]. [score:2]
Alterations in the serum levels of the miR371–3 and miR-302/367 MiRNAs also show promise as a diagnostic and follow-up tool for TGCT patients [38], highlighting the potential translational impact of molecular evaluation. [score:1]
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[+] score: 27
The expression levels and fold-changes of 35 most abundantly expressed miRNAs of T3/HDF and T3/CMHDF, as well as those of 31 miRNAs of T3/MEF and T3/CMMEF, cells are summarized in Table 2. These results indicate that nine hES cell-specific miRNAs (miR-302a, 302b, 302c, 302 d, 367, 371, 372, 373 & 200c) were abundantly expressed in T3/HDF and T3/CMHDF cells, and that miR-367 and miR-373 had little more than 2-fold variations between these two cell populations. [score:7]
Recently, we reported that the expression of hES cell-specific miRNAs miR-302 d, miR-372 and miR-367 and miR-200c, as well as miR-199a, were strongly up-regulated by activin A [6]. [score:6]
The undifferentiated state of T3/HDF and T3/CMHDF, as well as T3/MEF and T3/CMMEF, cells was evidenced by the very high expression levels of "stemness" genes, as well as hES cell-specific miR-302/367 and miR-371/372/373 clusters, and low expression levels of differentiation markers of ectoderm, mesoderm and endoderm in addition to the strong staining of OCT4 and NANOG. [score:5]
The extremely abundant expression of hES cell-specific miR-302/367 and miR-371/372/373 clusters also indicated the very high proportion of undifferentiated hES cells present in these four cell populations. [score:3]
It may also be noted that the miRNA data of T3/MEF and T3/CMMEF cells were previously determined using the set of 250 miRNAs in which miR-302a, 302b, 302c and 373 were not included, and that very similar expression profiles of miRNAs (r = 0.9624) between T3/MEF and T3/CMMEF cells were also found previously [6]. [score:3]
As demonstrated previously [24- 27], the miR-302/367 cluster on chromosome 4 and miR-371/372/373 cluster on chromosome 19 were extremely abundantly expressed in undifferentiated hES-T3 cells grown on T3HDF feeder (T3/HDF) and feeder-free Matrigel in T3HDF-conditioned medium (T3/CMHDF), as well as MEF feeder (T3/MEF) and feeder-free Matrigel in MEF-conditioned medium (T3/CMMEF). [score:3]
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[+] score: 26
When miR-302 cluster (without miR367) was overexpressed, significant increase in conversion of partial to fully reprogrammed iPS cells by suppressing MBD2 expression, thereby increasing Nanog expression was observed (19). [score:9]
Somatic cells reprogram to an embryonic stem cell (ESC) comparable induced pluripotent stem (iPS) cell state upon forced expression of exogenously delivered transcription factors, however, expression of exogenous miR-302 cluster (without miR-367) is efficient in attaining a fully reprogrammed iPS state. [score:5]
JMJD1C knockdown reduces miR-302 expression (36). [score:4]
It is thought that overexpressed miRNAs from the miR302/367 cluster in stem cells primarily repress development. [score:4]
The expression of the miR302/367 cluster rapidly and efficiently reprograms mouse and human somatic cells to an iPSC state without a requirement for exogenous transcription factors (10, 11). [score:3]
Eight miRNA loci (miR-302b, miR-302b [*], miR-302c, miR-302c [*], hsc-3, miR-302a [*], miR-302d, and miR-367) are located within an ~700-bp region on chromosome 4. Cell biology data indicated that the miR302-367 promoter activity depends on the ontogeny and hierarchical cellular stage. [score:1]
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33
[+] score: 24
Hsa-miR-424 had the highest degree of regulation in the MiRNA-Gene-Network; 37 target genes were down-regulated in the mRNA expression profiles, followed by hsa-miR-144 with 23 target genes and hsa-miR-302a, hsa-miR-181b and hsa-520d-3p, both had 20 target genes in the network. [score:13]
Among these 17 miRNAs, 7 miRNAs (hsa-miR-130b*, hsa-miR-21*, hsa-miR-223, hsa-miR-302a, hsa-miR-424, hsa-miR-451, hsa-miR-486-5p) were differentially expressed between active TB and latent TB, 6 miRNAs were up-regulated in active TB patients, and only hsa-miR-130b* showed reduced gene expression level. [score:8]
This difference between active and non-active TB groups was mainly due to the induced expression of hsa-miR-365, hsa-miR-223 and hsa-miR-302a, hsa-miR-486-5p, hsa-miR-144 and hsa-miR-451, hsa-miR-21* and hsa-miR-424 in active TB patients. [score:3]
[1 to 20 of 3 sentences]
34
[+] score: 24
Dysregulation of MIR101, MIR141, and MIR152 to the HIV-1 Gag protein contributes to HIV-1 budding and release via DNA hypermethylation, ubiquitin transfer, and endoplasmic reticulum -associated degradation at the late infection stage Briefly, dysregulation of; dysregulation of MIR9 contributes to HIV-1 infection to hijack CD4+ T cells through dysfunction of the immune and hormone pathways; dysregulation of MIR139-5p, MIRLET7i, and MIR10a contributes to the HIV-1 integration/replication stage through DNA hypermethylation and immune system dysfunction; dysregulation of MIR101, MIR141, and MIR152 contributes to the HIV-1 virus assembly/budding stage through DNA hypermethylation, ubiquitin transfer, and endoplasmic reticulum -associated degradation; dysregulation of MIR302a contributes to not only microvesicle -mediated transfer of miRNAs but also dysfunction of NF-κB signaling pathway in hepatocarcinogenesis. [score:7]
We found that dysregulation of; dysregulation of MIR9 contributes to HIV-1 infection to hijack CD4+ T cells through dysfunction of the immune and hormone pathways; dysregulation of MIR139-5p, MIRLET7i, and MIR10a contributes to the HIV-1 integration/replication stage; dysregulation of MIR101, MIR141, and MIR152 contributes to the HIV-1 virus assembly and budding mechanisms; dysregulation of MIR302a contributes to not only microvesicle -mediated transfer of miRNAs but also dysfunction of NF-κB signaling pathway in hepatocarcinogenesis. [score:6]
We identified that the change in the miRNA expression profile of MIR302a (p-value < 0.734) leads to the change in the gene expression profile of teashirt zinc finger homeobox 3 (TSHZ3) (p-value < 0.189), also known as zinc finger protein 537 (ZNF537), between normal liver cells and liver cancer cells (Fig.   4). [score:5]
D ysregulation of MIR302a contributes to not only microvesicle -mediated transfer of miRNAs but also dysfunction of NF-κB signaling pathway in hepatocarcinogenesis. [score:2]
D ysregulation of MIR302a contributes to not only microvesicle -mediated transfer of miRNAs but also dysfunction of NF-κB signaling pathway in hepatocarcinogenesisIt has been identified that microvesicles transfer miRNA between cells and alter biological functions by affecting signaling pathways in hepatocarcinogenesis [87]. [score:2]
Therefore, dysregulation of MIR302a contributes to not only microvesicle -mediated transfer of miRNAs but also dysfunction of NF-κB signaling pathway in hepatocarcinogenesis. [score:2]
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[+] score: 22
miR-302/miR-367 target several endogenous inhibitors of BMP and activin pathways including Lefty, TOB2, DAZAP2, and SLAIN1. [score:5]
More recently, miR-302 was shown to directly target NR2F2, a transcription factor involved in the very early triggering of the neural genetic program, suggesting that miR-302 may more specifically avoid a spontaneous commitment of PSCs into the neural lineage (Rosa and Brivanlou, 2011). [score:4]
miR-125, miR-302, and miR-371 both target proteins involved directly in signaling mediated by receptors of the TGF-beta family and modulate finely the strength of the signal transduction. [score:4]
Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP response. [score:3]
A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. [score:3]
Next to miR-302 family members, the miR-371/miR-372/miR-373 cluster is also considered as a potent inhibitor of the neural lineage commitment (Kim et al., 2011a). [score:2]
miRNAs of the miR-302/miR-367 family are particularly enriched in PSC. [score:1]
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[+] score: 22
On the other hand, miR-302 can enhance reprogramming by accelerating mesenchymal-to-epithelial translation (MET) [30] and suppressing some epigenetic regulators [23]. [score:6]
Nanog, Oct3/4, Sox2 and Rex1 are upstream regulators of the miR-302 cluster promoter [26], miR-302a regulate ESCs cell cycle by repressing cyclin D1 [27], and miR-302 impair early differentiation by repress NR2F2 [28] and Lefty [29] at the post-transcriptional level. [score:3]
We also analyzed miRNA cluster expression patterns and the results revealed that miRNAs from miR-302 cluster, miR-182/miR-183/miR-96 cluster and miR-143/miR-145 cluster were the most rpESCs enriched miRNAs. [score:3]
For miRNAs which had a very high expression level(>10000 reads TPM) in both parthenogenetic and IVF rhesus monkey ESCs, members of mir-302 cluster, miR-106b-25 cluster have important roles in maintaining of pluripotency. [score:3]
This phenomenon recently observed also in mouse and human parthenogenetic ESCs [1], [21], [22], due to homologous crossing-over and recombination during meiosis I. In our two rpESCs, mir-302 cluster had a very high expression level, consisting with previous studies that mir-302 cluster was high-riched in human ESCs [12]– [18]. [score:3]
Recently, the roles of mir-302 cluster in regulation of ESCs pluripotency have been clarified on some extent. [score:2]
Our previous research suggested that rhesus monkey ESCs from in vitro fertilization (IVF) blastocysts also share a unique miRNAs set, miR-302 cluster, which were reported to be highly enriched in human ESCs, were the most rhesus monkey ESCs enriched miRNAs [19]. [score:1]
Intriguingly, mir-302 cluster can reprogram cancer or somatic cells into iPS [23]– [25] revealed that mir-302 cluster had important roles in maintaining pluripotency of pluripotent cells. [score:1]
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[+] score: 21
Moreover, it has been recently reported that the NODAL inhibitor, LEFTY2, is down-regulated by miR-302, a microRNA that is highly enriched in hESC, thus revealing that modulating LEFTY2 at the translational level might be important to promote the undifferentiated stage [59]. [score:8]
miR-302 inhibits the translation of mRNAs inducing differentiation, including NR2F2 (an antagonist of OCT4), ZEB1 (Inhibitor of E-CADHERIN)and BMPR2 (inducing SMAD1/5/8 signalling) [22]– [24]. [score:7]
In human, OCT4, SOX2 and NANOG promote the expression of stem-cell enriched miRNAs, for example, the polycistronic miR-302/367 cluster [21]. [score:3]
BMP4 is a negative regulator of miR-302/367 [23]. [score:2]
Not surprisingly, a higher reprogramming efficiency has been achieved using a combination of OCT4, SOX2, KLF4 and MYC together with miR-302/367 [25]. [score:1]
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[+] score: 21
MicroRNA miR-302 inhibits the tumorigenecity of human pluripotent stem cells by coordinate suppression of the CDK2 and CDK4/6 cell cycle pathways. [score:5]
Regulation of somatic cell reprogramming through inducible mir-302 expression. [score:4]
0127119.g001 Fig 1 A) Relative expression of endogenous miR-302s (miR-302a,-b,-c, and-d), miR-369s (miR-369-3p and-5p), and miR-200c in primary tumors and colon cancer cell lines. [score:3]
A) Relative expression of endogenous miR-302s (miR-302a,-b,-c, and-d), miR-369s (miR-369-3p and-5p), and miR-200c in primary tumors and colon cancer cell lines. [score:3]
S2 Fig A, Fluorescent TUNEL staining was performed to detect apoptotic HT29 cells transfected with miR302, miR302 plus miR-369s, or negative control (NC) miR. [score:1]
C, of the apoptosis-related proteins Bak, Bid, Bcl-xl, Bcl2, Mcl1, Caspase-8, and Caspase-3 in HT29 and DLD-1 cells transfected with miR302, miR-369s, miR302 plus miR-369s, or NC miR. [score:1]
Previous studies indicated that miR-302a,-b,-c, and-d (miR-302s), miR369-3p,-5p (miR-369s) and miR-200c could elicit cellular reprogramming in normal somatic cells [13]. [score:1]
miR302 -transfected DLD-1 colorectal cancer cells were analyzed for DNA methylation. [score:1]
B, Propidium iodide and Annexin V-FITC staining was performed in DLD-1 cells 60 h post-transfection with miR302, miR-369s, miR302 plus miR-369s, or NC miR. [score:1]
To prepare a CA transfection mixture in vitro, 2 μg of each miR-302 (-a,-b,-c,-d), miR-369 (-3p,-5p) or NC miR was mixed with 4 μL of 1 M CaCl [2] in 1 mL of serum-free bicarbonate (44 mM)-buffered DMEM medium (pH 7.5) incubated at 37°C for 30 min, and used for transfection [15– 17]. [score:1]
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[+] score: 21
Oct4 can bind directly to miR-302 and upregulate its expression [171], while canonical Wnt signaling regulates mir-302 expression involving 3 TCF/LEF binding sites. [score:10]
In the latter, knocking down β-catenin leads to decreased expression of mir-302, whereas knocking down Tcf3 produces the opposite effect [174], which promotes the expression of pluripotency genes in F9 and P19 cells [154, 175]. [score:7]
In one case this regulation involves the mir-302 gene, which encodes a cluster of 5 microRNAs (miRNAs) that are highly expressed in undifferentiated NTERA-2 cells and P19 cells [172, 173]. [score:4]
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[+] score: 21
Of these, 271 miRNAs were overexpressed in hESCs and 73 were overexpressed in HFF-1. The fifteen most significant miRNAs derived from eleven different hairpin precursors, all overexpressed in the hESC libraries, are shown in Table 2. Ten of these miRNAs belong to known hESC-specific miR-302/367 and miR-371/372/373 clusters. [score:7]
Seven of the significantly differentially expressed moRNAs were derived from the hESC-specific miR-302/367 cluster. [score:3]
Overexpression of miR-302 family members is able to reprogram human and mouse somatic cells to pluripotency [19, 20]. [score:3]
Most miRNAs deriving from the well-known pluripotency related miR-302/367, miR-371/372/373 and C19MC clusters were significantly overexpressed in our hESC data, a finding that is well in line with the earlier observations. [score:3]
miRNAs found in hESCs belong mostly to the miR-302 and miR-290 families expressed from miR-302/367 and miR-371–373 clusters, respectively [13, 14]. [score:3]
Among them are seven of the ten moRNAs that derive from the pluripotency related miR-302/367 cluster. [score:1]
One of those fluctuating TFs is NANOG [73], which is also involved in activation of ES cell miRNAs miR-290 and miR-302 [74]. [score:1]
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These data indicated that hiPSCs highly expressed miR-302 and miR-17, whereas NHDFs highly expressed let-7 accompanied by modest levels of miR-17 expression. [score:7]
Subsequent incorporation of four copies of the target sequence for miR-302a, let-7a, or miR-17 into the 3′ UTR of KR or EGFP created two different sensor vectors, SeVdp-302aT/let-7aT and SeVdp-302aT/17T (Fig.   4a), with SeVdp-302aT/let-7aT capable of being used to simultaneously evaluate miR-302 and let-7 expression, and SeVdp-302aT/17T capable of being used to simultaneously evaluate miR-302 and miR-17 expression. [score:3]
Although this system allowed long-term monitoring of miR-302a-5p expression during hiPSC differentiation, episomal vectors may be rapidly cleared from highly proliferating cells [46]. [score:3]
Quantitative RT-PCR (qRT-PCR) analysis indicated that both miR-302a and miR-124 were expressed in hiPSCs (Supplementary Fig.   S2a,b). [score:3]
Particularly high levels of miR-302a expression were detected in hiPSCs as compared with levels observed in normal human dermal fibroblasts (NHDFs), H9-derived neural stem cells (H9-NSCs), and Wharton’s jelly stem cells (WJSCs). [score:2]
To investigate the potency of SeVdp-miR-Sensor, we constructed vectors containing target sequences for let-7a (SeVdp-let-7aT), miR-302a (SeVdp-302aT), miR-9 (SeVdp-9T), or miR-124 (SeVdp-124T), as well as a vector containing complementary sequences for a portion of the firefly luciferase gene (SeVdp-FlucT) as a control. [score:1]
Four copies of the complementary sequence for miR-302a, miR-17, or let-7a were incorporated into the 3′ UTRs of the KR and EGFP genes to construct the SeVdp-302aT/let-7aT and SeVdp-302aT/17T vectors. [score:1]
miR-302a, miR-122, and miR-208a can be used to specifically identify embryonic stem cells (ESCs), hepatocytes, and cardiomyocytes, respectively 5, 8, 13, and, interestingly, miR-375 can be used as a marker for isolation of insulin-producing cells lacking available specific surface markers [8]. [score:1]
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[+] score: 20
Other miRNAs from this paper: hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-302e, hsa-mir-302f
MiR-302 indeed belongs to a class of miRNAs that functions as cytoplasmic gene silencers: this is in suppressing translation of targeted messenger RNAs (mRNA). [score:7]
A majority of miR-302 -targeted genes are transcripts involved in development-related signals and oncogenes [131]. [score:4]
In using a vector which expresses a cDNA encoding for miR-302 and further selecting cells for its stable integration with antibiotics, Lin and co-workers [30] were able to achieve full reprogramming of cells from human hair follicles; however that cells are slow to propagate because of a restricted cell cycle rate [134]. [score:3]
In human, miR-302 is predominantly expressed in hES and iPS cells, but not in differentiated cells [132, 133]. [score:3]
Finally, recent studies have evidenced that both Oct4 and Sox2 play a pivotal role in miR-302 expression in human embryonic stem cells (hES) [129, 130]. [score:3]
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[+] score: 20
QPCR confirms the result of network analysis as MIR302A showed significant upregulation and MIR Let7g showed significant downregulation in Human Amniotic Fluid Stem Cells (hAFSCs). [score:7]
Regulation of somatic cell reprogramming through inducible mir-302 expression. [score:4]
Expression analysis of MIR302A, MIRLet7G, MIR 21, and MR 137 in Human Amniotic Fluid Stem Cells. [score:3]
Interestingly, Common Targets algorithm highlighted the possible function of 2 microRNAs including MIR302A and MIRLET7G (Figs 8 and 9) as these microRNAs interact with NANOG and POUSF1 and also are involved in 2 differentiation process of adipogenesis and osteogenesis. [score:3]
Interestingly, Mir302A which was a hub (central component) in the network constructed by the Common Targets algorithm generate a significant subnetwork with high chance of generation at P<0.001. [score:2]
MIR302A and MIRLET7G, MIR 21, and MR 137 were selected for validation using qPCR. [score:1]
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[+] score: 20
Real-time PCR confirmed microarray analysis results: expression of miR-302a and miR-491-3p was up-regulated (Fig. 2) and miR-520d-3p and miR-383 was down-regulated (Fig. 2) in patients with NOA. [score:9]
Quantitative real-time PCR analysis confirmed microarray data: miR-302a and miR-491-3p was up-regulated and miR-520d-3p and miR-383 was downregulated in NOA patients. [score:7]
For example, one of the predicted target genes of miR-302a and miR-383 is MLH1, while miR-491-3p and miR-520d-3p is SCP1 and SCP3, respectively. [score:3]
To confirm the results obtained by microarray analysis, quantitative real-time RT-PCR analysis of normal and NOA testicular samples was performed for miR-302a, miR-491-3p, miR-520d-3p and miR-383. [score:1]
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[+] score: 20
The mir-31 [36]– [38], mir-302a [39], [40], mir-21 [41], mir-181b [41], [42] and were found to be up-regulated in several cancers. [score:4]
In addition, the bio-informatic results revealed many potential pre-miRNA sequences including pre-mir-302a and pre-let-7b in the mitochondrial genome. [score:1]
Using 2.5 pmol of locked nucleic acid (LNA) probes prelabelled with digoxigenin, we determined in situ hybridization patterns of scramble miR (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 1). [score:1]
The most significant alignments with human miRNA were obtained with four pre-miRNA (pre-mir-302a, pre-let-7b, pre-mir-1267 and pre-mir-1296; E-value <0.1) and with the two miRNA (mir-365 and mir-31; E-value <0.1) Examples of alignment results show that for some candidates the seed region was perfectly aligned and some others had one or two nucleotide differences (Figure 1). [score:1]
In situ hybridization of pre-mir-302a, pre-let-7b and mir-365, using specific labelled locked nucleic acids and confocal microscopy, demonstrated that these miRNA were localized in mitochondria of human myoblasts. [score:1]
Using locked nucleic acid (LNA) probes, we performed in situ hybridization to localized mir-365, mir-let-7b, pre-let-7b and pre-mir-302a within the cell. [score:1]
The in situ hybridization clearly localized two pre-microRNA (pre-mir302a and pre-let-7b) and one microRNA (mir-365) in the mitochondria. [score:1]
Strikingly, we also observed that two pre-miRNAs, pre-mir302a and pre-let-7b were located within mitochondria surrounding the nucleus (Figures 5E, F). [score:1]
0020220.g005 Figure 5Using locked nucleic acid (LNA) probes, we performed in situ hybridization to localized mir-365, mir-let-7b, pre-let-7b and pre-mir-302a within the cell. [score:1]
As it circumvents the need for mitochondrial purification, this approach helped to clearly establish the localization of at least two pre-miRNA (prem-mir-302a and pre-let-7b) and one miRNA (mir-365) in human mitochondria. [score:1]
The present study demonstrated for the first time the localization of pre-miRNA (pre-mir-302a, pre-let-7b) and miRNA in human mitochondria isolated from muscular cells. [score:1]
Hoechst 33342 staining of nuclei (lane 1), specific signal of scramble miR (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) provided by locked nucleic acid (LNA) probes (lane 2) and MitoTracker® Red CM-H [2]XRos staining of functioning mitochondria (lane 3) are represented in gray scale. [score:1]
In situ signal of mir-365, pre-mir-let7b and pre-mir-302a co-localized with functioning mitochondria in human myoblasts observed in confocal microscopy. [score:1]
The hypothesis of mitochondrial miRNA synthesis could be supported by the present results of co-localization of pre-mir-302a and pre-let-7b in the mitochondria. [score:1]
0020220.g004 Figure 4 Using 2.5 pmol of locked nucleic acid (LNA) probes prelabelled with digoxigenin, we determined in situ hybridization patterns of scramble miR (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 1). [score:1]
Mir-365, pre-mir-302a and pre-let7b specific fluorescent LNA were clearly co-localized in perinuclear mitochondria, as demonstrated by the yellow signal one could observed in that area (Figure 3D, E, F). [score:1]
Hoechst 33342 staining of nuclei (lane 1), specific signal of scramble miRNA (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 2) and MitoTracker® Red CM-H [2]XRos staining of respiring mitochondria (lane 3) are represented in gray scale. [score:1]
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[+] score: 20
Irrespective of p53 status, after radiation miR-302a and miR-302c up-regulated, and miR-518f down-regulated in the all cell lines, whereas after SN38 treatment up-regulated miRNAs were miR-133a, miR-155-3p, miR-204, miR-22, miR-512-3p, miR-517a, miR-517c and miR-708 in the all cell lines (Figure 3, Table 1a). [score:10]
Irrespective of wild type or mutated or null p53, after radiation treatment, miR-302a and miR-302c up-regulated, and miR-518f down-regulated in colon cancer cells, whereas after SN38 treatment up-regulated miRNAs were miR-133a, miR-155, miR-204, miR-22, miR-512-3p, miR-517a, miR-517c and miR-708 in the all colon cancer cell lines. [score:10]
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[+] score: 19
We chose these miRNAs because they are among the most abundant miRNAs expressed in hESCs (miR-302 and 367) or NSCs (miR-9–1) and because their isomiRs are co-expressed at levels that are comparable to most of the canonical miRNAs in our libraries (Supplementary Table S2). [score:5]
5′ and 3′ isomiRs are functional in vitroIn order to further test whether isomiRs are functional, we constructed luciferase reporter vectors with the 3′UTR mRNA of potential targets of miR-9, miR-302a, miR367 and their corresponding isomiRs (Supplementary Table S4). [score:3]
is expressed as a canonical miRNA by mmu-miR-302c but as an isomiR by hsa-miR-302c and all of the remaining members of the miR-302 family of man and mouse. [score:3]
In order to further test whether isomiRs are functional, we constructed luciferase reporter vectors with the 3′UTR mRNA of potential targets of miR-9, miR-302a, miR367 and their corresponding isomiRs (Supplementary Table S4). [score:3]
In addition, the 3′UTRs of BTG1 and HMGA2 were confirmed as predicted targets of both miRNAs and 5′isomiRs of miR-302a and miR-9, respectively. [score:3]
Membranes were washed twice at room temperature with 2 × SSC and 0.1% SDS for 5 min and exposed on x-ray film with an intensifying screen at −80°C for a minimum of 48 h. Digoxigenin labelled locked nucleic acid probe (Exiqon) specific to miR-9, miR-302a and let-7d were used in some of the hybridization experiments. [score:1]
Figure 1E illustrates that the ratios of the isomiR bands for miR-9 and miR-302a that we detected by northern blotting correspond well with the sequencing results. [score:1]
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[+] score: 17
The relative expression of miRNAs in DCM samples and healthy control samples is shown in Figure 9. We found a 2.6-fold increase in hsa-miR-340 expression (P < 0.001), a 2.4-fold increase in hsa-mir-19b expression (P < 0.01) and a twofold increase in hsa-miR-302 expression (P < 0.05) in DCM samples. [score:9]
The hsa-miR-19b and hsa-miR-302 were down-regulated in the profile analysis, but up-regulated in the quantitative RT-PCR assay. [score:6]
The miRNA-302 family plays a role in regulating growth factor signalling pathways, with implications for nephropathic cell fate transitions [37]. [score:2]
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[+] score: 16
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-20a, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-27a, hsa-mir-30a, hsa-mir-93, hsa-mir-96, hsa-mir-99a, hsa-mir-100, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-105-1, hsa-mir-105-2, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-10a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-205, hsa-mir-212, hsa-mir-181a-1, hsa-mir-222, hsa-mir-224, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-27b, hsa-mir-30b, hsa-mir-122, hsa-mir-125b-1, hsa-mir-132, hsa-mir-141, hsa-mir-145, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-146a, hsa-mir-150, hsa-mir-184, hsa-mir-188, hsa-mir-320a, hsa-mir-181b-2, hsa-mir-30c-1, hsa-mir-34c, hsa-mir-30e, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-371a, hsa-mir-372, hsa-mir-376a-1, hsa-mir-378a, hsa-mir-383, hsa-mir-339, hsa-mir-133b, hsa-mir-345, hsa-mir-425, hsa-mir-483, hsa-mir-146b, hsa-mir-202, hsa-mir-193b, hsa-mir-181d, hsa-mir-498, hsa-mir-518f, hsa-mir-518b, hsa-mir-520c, hsa-mir-518c, hsa-mir-518e, hsa-mir-518a-1, hsa-mir-518d, hsa-mir-518a-2, hsa-mir-503, hsa-mir-513a-1, hsa-mir-513a-2, hsa-mir-376a-2, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-548c, hsa-mir-645, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-378d-2, hsa-mir-744, hsa-mir-548e, hsa-mir-548j, hsa-mir-548k, hsa-mir-548l, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-302e, hsa-mir-302f, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, hsa-mir-548q, hsa-mir-548s, hsa-mir-378b, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-548w, hsa-mir-320e, hsa-mir-548x, hsa-mir-378c, hsa-mir-548y, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-378f, hsa-mir-378g, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-378h, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-378i, hsa-mir-548am, hsa-mir-548an, hsa-mir-371b, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-378j, hsa-mir-548ay, hsa-mir-548az, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-548bc
Interestingly, some miRNAs such as miR-29b and miR-430 overlapped between different vertebrate species and some miRNAs upregulated (miR302a) or downregulated (Let-7) in germ cell tumors overlapped with miRNAs identified in vertebrate PGCs according to different studies, as can be seen in Figure 1. MiRNAs identified in PGCs and germ cell tumors differ from miRNAs that were found to be abundant in mature human oocytes [28, 29]. [score:7]
It has been shown that all malignant germ cell tumors overexpress the miR-371–373 and miR-302/367 clusters with increased serum levels, regardless of patient age, histological subtype, or anatomical site of tumor [26, 27]. [score:3]
The miR-302/367 cluster represents the most abundant cluster of eight miRNAs that are specifically expressed in hESCs [81]. [score:3]
Functional studies identified important roles of miR-302/367 in regulation of pluripotency and differentiation of hESCs in vitro. [score:2]
Beside its role in TGF- β signaling, miR-302/367 also promotes the bone morphogenetic protein (BMP) signaling. [score:1]
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Expression of the pluripotency -associated miRNAs miR-302a–d, -372, and -373 was significantly suppressed with progression of differentiation (Supporting Information Fig. S1B, S1C). [score:5]
Expression of mature miR-302a-d, miR-372 and miR-373 is suppressed in an endothelial differentiation-specific manner, as compared to time-matched SA461 pluripotent samples. [score:4]
Indeed, it has recently been demonstrated that overexpression of the miR-302 cluster can facilitate reprogramming of somatic cells to pluripotency [16]. [score:3]
Pluripotent human embryonic stem cells (hESCs) are reported to express a unique panel of miRNAs, including the miR-302 cluster and miR-371/372/373 cluster [12, 13]. [score:3]
We also validated the substantial loss of differentiation -associated miR-302a–d, -372, and -373 in agreement with previous studies [12, 13]. [score:1]
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Other miRNAs from this paper: hsa-mir-16-1, hsa-mir-17, hsa-mir-20a, hsa-mir-21, hsa-mir-23a, hsa-mir-100, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-107, hsa-mir-16-2, mmu-mir-1a-1, mmu-mir-23b, mmu-mir-125b-2, mmu-mir-130a, mmu-mir-9-2, mmu-mir-145a, mmu-mir-181a-2, mmu-mir-184, mmu-mir-199a-1, hsa-mir-199a-1, mmu-mir-205, mmu-mir-206, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-199a-2, hsa-mir-205, hsa-mir-181a-1, hsa-mir-214, hsa-mir-219a-1, hsa-mir-223, mmu-mir-302a, hsa-mir-1-2, hsa-mir-23b, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-145, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-184, hsa-mir-206, mmu-mir-16-1, mmu-mir-16-2, mmu-mir-20a, mmu-mir-21a, mmu-mir-23a, mmu-mir-103-1, mmu-mir-103-2, rno-mir-338, mmu-mir-338, rno-mir-20a, hsa-mir-1-1, mmu-mir-1a-2, hsa-mir-181b-2, mmu-mir-107, mmu-mir-17, mmu-mir-100, mmu-mir-181a-1, mmu-mir-214, mmu-mir-219a-1, mmu-mir-223, mmu-mir-199a-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-181b-1, mmu-mir-125b-1, hsa-mir-219a-2, mmu-mir-219a-2, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-367, hsa-mir-372, hsa-mir-338, mmu-mir-181b-2, rno-mir-9a-1, rno-mir-9a-3, rno-mir-9a-2, rno-mir-16, rno-mir-17-1, rno-mir-21, rno-mir-23a, rno-mir-23b, rno-mir-100, rno-mir-103-2, rno-mir-103-1, rno-mir-107, rno-mir-125b-1, rno-mir-125b-2, rno-mir-130a, rno-mir-145, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-184, rno-mir-199a, rno-mir-205, rno-mir-206, rno-mir-181a-1, rno-mir-214, rno-mir-219a-1, rno-mir-219a-2, rno-mir-223, hsa-mir-512-1, hsa-mir-512-2, rno-mir-1, mmu-mir-367, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, rno-mir-17-2, hsa-mir-1183, mmu-mir-1b, hsa-mir-302e, hsa-mir-302f, hsa-mir-103b-1, hsa-mir-103b-2, rno-mir-9b-3, rno-mir-9b-1, rno-mir-9b-2, rno-mir-219b, hsa-mir-23c, hsa-mir-219b, mmu-mir-145b, mmu-mir-21b, mmu-mir-21c, mmu-mir-219b, mmu-mir-219c, mmu-mir-9b-2, mmu-mir-9b-1, mmu-mir-9b-3
For example, the cluster of miR-512-3p, miR-205, and the miR-302 family illustrated on the heat map demonstrates high expression in undifferentiated ES and early neural progenitor stages, downregulation during the glial restricted and early OP transitions, but then additional high expression during mid to late OP development. [score:9]
From the top ten downregulated miRNAs at this transition, six miRNAs (miR-367, miR-302a, miR-302c, miR-372, miR-302b, and miR-302d) have been shown to encourage proliferation and are highly expressed in undifferentiated cells and cancer stem cells [43], [44], [45], [46], [47], [48]. [score:6]
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In fact, miR-302 and miR-372 have been shown to promote reprogramming, and miR-302 and miR-372 inhibit the TGF-ß -induced epithelial–mesenchymal transition, as does mouse miR-294 [37]. [score:3]
Eigenvectors with absolute values greater than 2 are shown in Table 2. Most of the listed miRNAs are members of the miR-302 cluster and two human-specific C19MC clusters (the miR-371/372/373 and miR-512∼ clusters), and these miRNAs were previously reported to be expressed in ES and iPS cells [10], [12]– [16], [30], [31]. [score:3]
In addition, other miRNAs—such as miR-17, -20a, -93, -106b, -106a, and -20b, and miR-302 members—which share the same sequence as the miR290 cluster, the miR-371 cluster, and C19MC, and were detected at high levels in both human and mouse ES/iPS cells, were shown to mediate reprogramming by targeting Tgfßr2 and p21 during the mesenchymal-to-epithelial transition during the initiation stage of reprogramming [6]. [score:3]
The miRNAs, reported in numerous studies and expressed abundantly in human and mouse pluripotent cells, are members of the miR-302 cluster [12], [13], [15], [16]. [score:3]
The seed sequence of miR-302 is identical to those of miR-372, miR-373 [39], [40], and some C19MC members. [score:1]
Members of the miR-302 cluster were ranked 11 [th], 17 [th], 34 [th], and 59 [th] in the mouse list, and 1 [st], 30 [th], and 33 [rd] in the human list. [score:1]
In addition, miR-367, which is a distantly related member of the miR-302 cluster [36], was 37 [th] in the mouse list and 17 [th] in the human list. [score:1]
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These miRNAs were differentially-expressed upon NaB -induced differentiation and represent ES miRNAs (hsa-miR-302a*, hsa-miR-302d, hsa-miR-517b), endodermal miRNAs (hsa-miR-122, hsa-miR-375) and miRNAs that were upregulated in both lines (hsa-miR-10a, hsa-miR-24). [score:6]
Further, a few hepatic/endoderm markers such as FOXA2, Alpha-fetoprotein, Albumin (Fig. 7D–F) and miR-375 (Fig. 6B) were not upregulated, and there was a concomitant increase in the expression of the ESC-specific miR-302a* (Fig. 6C). [score:6]
Characterizations of miRNA expression in mouse [13]– [16] and in human [17]– [20] ES cells and ESC-derived embryoid bodies have been recently published, and revealed two highly-expressed clusters (miR-302 and mmu-miR-290/hsa-miR-371/372/373). [score:3]
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Pluripotency-related miRNA (i. e., miR-290 and miR-302 clusters) also regulate reprogramming through suppression of differentiation-related miRNA (i. e., let-7 family, miR-21) [93], with Oct4 and Nanog directly regulating their expression [93]. [score:8]
Indeed, MEFs nearing senescence (late passage) are poorly reprogrammed, and the microRNA families miR-290 and miR-302, which inhibit senescence by inhibiting the expression of G1/S checkpoints, improve their reprogramming [20, 21]. [score:7]
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Although clustered miRNA family members tend to have similar expression patterns, in this study miR302a* was expressed in baboon liver and lymphocytes while 302a was not detected. [score:5]
We confirmed expression of miR21, 26b, 30a-5p, 760, and 16-1 (Figure 2) and lack of detectable expression for miR302a, 648, and 373. [score:5]
For example, miRNA cluster members on chr 19 were down regulated while miR302a and 302d gene family members on human chr 4 and miR17, 18, and 19 on chr 13 [37] were expressed in baboon liver and lymphocytes. [score:4]
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Lin S. L. Chang D. C. Ying S. Y. Leu D. Wu D. T. MicroRNA miR-302 inhibits the tumorigenecity of human pluripotent stem cells by coordinate suppression of the CDK2 and CDK4/6 cell cycle pathways Cancer Res. [score:5]
Lin S. L. Chang D. C. Lin C. H. Ying S. Y. Leu D. Wu D. T. Regulation of somatic cell reprogramming through inducible miR-302 expression Nucleic Acids Res. [score:4]
Subramanyam D. Lamouille S. Judson R. L. Liu J. Y. Bucay N. Derynck R. Blelloch R. Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells Nat. [score:3]
Koga C. Kobayashi S. Nagano H. Tomimaru Y. Hama N. Wada H. Kawamoto K. Eguchi H. Konno M. Ishii H. Reprogramming using microRNA-302 improves drug sensitivity in hepatocellular carcinoma cells Ann. [score:1]
The miR-302b lies in the miR-302-367 cluster, where else includes miR-302c, miR-302a, miR-302d and miR-367 [19]. [score:1]
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Although miR-302 families have been suggested to be tumor repressors in human cancer, the mechanism by which they suppress tumor development remains to be defined. [score:4]
Although miR-302 has been suggested to have tumor suppressor potential, the present studies focused on the self-renewal and proliferation properties of miR-302b in the stemness maintenance of embryonic stem cells (ESCs) or tumor stem cell properties in advanced cancer cells [17, 18]. [score:3]
Other studies have demonstrated the tumor suppressive activity of miR-302 in human pluripotent stem cell by both the CCNE-CDK2 and CCND-CDK4/6 pathways in G1-S cell cycle transition. [score:3]
The miR-302 family consists of four highly-homologous miRNA members, which are transcribed together as a noncoding RNA cluster containing mir-302b, mir-302c, mir-302a, mir-302d, and mir-367 in a 5′-to-3′ direction [16]. [score:2]
To date, miR-302 s have been proven to post-transcriptionally regulate CCND1 and CDK4, therefore affecting cell cycle progression. [score:2]
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Faherty N Curran SP O’Donovan H Martin F Godson C Brazil DP CCN2/CTGF increases expression of miR-302 microRNAs, which target the TGFβ type II receptor with implications for nephropathic cell phenotypesJ Cell Sci. [score:5]
Our findings also demonstrate that miR-299, miR-499, miR-302, and miRNA-200 were upregulated in EPO-MVs (Fig.   8c). [score:4]
The miRNA profiles of the MVs revealed that EPO-MVs changed 212 miRNAs (fold-change ≥ 1.5), including miR-299, miR-499, miR-302, and miRNA-200, and that 70.28 % of these changes involved upregulation. [score:4]
miR-302 decreases TGF-β1 -induced EMT in renal HKC8 epithelial cells and attenuates the TGF-β1 -induced mesangial production of fibronectin and thrombospondin [44]. [score:1]
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In our exon-array gene expression analysis, genes for 26 miRNAs were up- or downregulated during differentiation to CPs and NPs, including previously implicated pluripotency (mir-302a, 302b) [61] and cardiac (mir-133, 23b, 26a) [44] regulated miRNAs (Dataset S1). [score:7]
1000553.g007 Figure 7 (A) Expression profiles of two previously characterized miRNAs, mir-302a and mir-133-1, from combined tissue/cell-line gene expression data. [score:3]
Examination of miRNAs with previously established hESC or cardiac differentiation expression patterns identified binding sites for mir-302a, 302c (ESC), and mir-26a (cardiac) in the alternative bleeding exon of SPTBN1 and the afore mentioned binding sites in the 3′UTR of ATP2A2. [score:3]
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[+] score: 13
Some microRNAs such as miR-145 and miR-302a can inhibit OCT4 translation and regulate OCT4 gene expression during development, respectively [14], [15]. [score:9]
Card DA Hebbar PB Li L Trotter KW Komatsu Y Mishina Y Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells. [score:4]
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Curcumin was shown to significantly downregulate the H [2]O [2] -induced expression of miR-302 cluster in ARPE-19 cells. [score:6]
Based on statistical significance (p<0.05) and 2 -FC, curcumin pretreatment attenuated the H [2]O [2] -induced expression of 17 miRNAs (miR-15b, miR-17, miR-21, miR-26b, miR-27b, miR-28–3p, miR-30b, miR-30d, miR-92a, miR-125a-5p, miR-141, miR-196b,, miR-195, miR-302a, miR-302c, miR-320a, and miR-9), which were also significantly reduced by the curcumin treatment alone (Figure 4, Table 2). [score:3]
MiR-302 has been reported to inhibit several epigenetic regulators, including AOF1/2, methyl-CpG binding proteins 1 and 2, and DNA (cytosine-5-)-methyltransferase 1, that induce global DNA demethylation and subsequently activate transcription factors Oct4, Sox2, and Nanog [26]. [score:3]
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In one example, mir-302 is induced by Oct4 (POU5F1) and Sox2 in hESC, in turn suppressing cyclin D1 and thereby (along with other target mRNAs), increasing the fraction of cells in S phase and stimulating cell cycle [7]. [score:5]
Two clusters (2 and 6) included microRNAs primarily expressed in pluripotent cultures and these clusters included mir-302 family members as expected. [score:3]
For example, predicted microRNA chr16:62121639-62121678:- was based on RNA sequences found in ESC and its expression clusters with mir-302a, with which it shares a seed sequence (cluster 2, see Figure S7). [score:3]
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92) 43 hsa-mir-302a dbDEMC 19 hsa-let-7g dbDEMC, miR2Disease 44 hsa-mir-212 literature 20 hsa-let-7b dbDEMC, HMDD, miR2Disease 45 hsa-mir-372 dbDEMC 21 hsa-mir-150 dbDEMC, literature 46 hsa-mir-197 dbDEMC 22 hsa-mir-338 dbDEMC 47 hsa-mir-124 literature 23 hsa-mir-103 dbDEMC, miR2Disease 48 hsa-mir-378 HMDD 24 hsa-mir-15b dbDEMC, HMDD 49 hsa-mir-26b dbDEMC, miR2Disease 25 hsa-mir-31 dbDEMC, HMDD, miR2Disease 50 hsa-mir-542 higher RWRMDA (No. [score:11]
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miRNA target has-miR302a MECP2 hsa-miR29a TET1, TET2, TET3 has-miR29a/c DNMT3A, DNMT3B has-miR29b-1/2 DNMT1 (Indirect via SP1) hsa-miR148a DNMT3B hsa-miR148a DNMT1 hsa-miR152 DNMT1 has-miR302a DNMT1 (Indirect via AOF2) hsa-miR342 DNMT1 hsa-miR17-92 DNMT1 hsa-miR26a-1/2 EZH2 hsa-miR101-1/2 EZH2/EED hsa-miR214 EZH2 hsa-miR128-1/2 BMI-1 hsa-miR199a-1/2 BRM hsa-miR433 HDAC6 hsa-miR449a HDAC1 hsa-miR138 SIRT1In the first column we report a list of miRNAs which are known to target epigenetic regulators and in the second column the corresponding targets. [score:10]
It is well known (see for instance Gruber and Zavolan, 2013) that Dnmt proteins are strictly controlled in a coordinated way by a number of miRNAs, among them in particular mir-29a/b/c, mir-152, mir148a, mir342, mir302 and various members of the cluster mir17-92. [score:1]
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Functionally, the miR-302 cluster has been associated with cellular reprogramming where iPS cells overexpressing miR-302 exhibited suppressed MBD2 expression which in turn increased expression of NANOG (Lee et al., 2013). [score:9]
Epigenetic Regulation of Nanog by MiR-302 Cluster-MBD2 Completes Induced Pluripotent Stem Cell Reprogramming. [score:1]
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Treatment with miR-27a/b [46], miR-128 [49], miR-145 [50], miR-302 [51], and miR-758 [48] directly suppressed ABCA1 by binding to its 3' UTR and attenuated cholesterol efflux to ApoA1. [score:4]
Inhibition of miR-302a also increased plasma HDL, decreased plaque formation, and promoted lesion remo deling. [score:3]
These results highlight the potential of miR-33 or miR-302a suppression as a strategy to promote RCT and the regression of atherosclerosis [51, 55]. [score:3]
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In addition, miR-302 and miR-467a are expressed in mouse ES cells and supplement silencing of the miR-290–295 targets via the 2–7 U seed [16], [27]. [score:5]
The miR-302 cluster is the most abundant miRNA family in human ES cells, and appears to be mostly responsible for 2–7 U seed functions instead of miR-371–373, which is expressed at much lower levels [12]. [score:3]
This seed is shared with miRNAs that are otherwise unrelated such as the miR-430, miR-302 and miR-467a families (http://www. [score:1]
miR-430 and miR-302 appear in the zebrafish and chick genomes and have therefore been acquired before the split of the mammalian lineage. [score:1]
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Other miRNAs from this paper: hsa-mir-23a, hsa-mir-27a
miR-27a-5p As Putative Regulator of CX [3]CR1 ExpressionThe differential expression of miR-302a and miR-27a-5p in untreated and TGF-β1 -treated NK cells was checked for validation by using specific miRNA assays. [score:5]
The differential expression of miR-302a and miR-27a-5p in untreated and TGF-β1 -treated NK cells was checked for validation by using specific miRNA assays. [score:2]
[8] The remaining two miRNAs (miR-302a and miR-27a-5p) were further investigated as putative regulators of CXCR4, CXCR3, or CX [3]CR1 expression (Figure 1 and Figure S2 in). [score:2]
The analysis revealed that miR-302a was virtually absent in both untreated and TGF-β1 -treated NK cells (data not shown). [score:1]
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Genome-scale profiling of microRNA (miRNA) differential expression showed that the expression of pluripotence -associated hsa-miR-302 family was silenced and the expression of Hox miRNA hsa-miR-10 family that regulates gene expression predominantly in neuroectoderm was induced to high levels in those hESC-derived neuronal progenitors [16, 19]. [score:10]
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Scatter plots showing the number of miRNAs targeting an mRNA (Y axis) versus the correlation between miRNAs and mRNA expression (Y axis) for selected miRNA families within the integrated data set for neuroblastoma (a) the let-7 family and (b) and mir-302 family, and human immune cells (c) the let-7 family and (d) the mir-320 family. [score:5]
In Figure 9a, we see that hsa-miR-7 is a well-connected node within the module ‘Positive Regulation of Neuron Projection Development’ that is principally enriched for the mir-302a family/cluster (P < 1.9-04). [score:3]
Interestingly, although the mir-302 family showed cooperativity in the data set, it did not show any significant cooperativity in the Human Immune Cells data set. [score:1]
In some cases, there is limited evidence of a greater effect, such as in the plots of the mir-302 family in the neuroblastoma data set (Figure 5b) and the mir-320 family in the human immune cells data set (Figure 5d). [score:1]
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All combinations involve miR302, which has been shown to stimulate the expression of Oct4/ Sox2 and Nanog as well as inhibiting several factors that stimulate DNA methylation [73] and stimulating tumour suppressor related pathways [74]. [score:7]
MicroRNA’s have the advantage of specifically targeting multiple pathways and as seen for miR302 may therefore reduce the amount of factors to be introduced to induce pluripotency. [score:3]
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Quantitation of p21 protein levels reportedly regulated post-transcriptionally by miRNAs of the hsa-miR-302 family [23] revealed the expected down-regulation of this protein in IGROV-1 cells transfected with hsa-miR-302b precursor (data not shown), suggesting that hsa-miR-302b exerts biological effects in IGROV-1 cells similar to those observed in other cellular mo dels. [score:5]
Moreover, a very recent study reports direct regulation of p21 protein by members of the miR-302 family activated following DNA damage in human embryonic stem cells [20], further suggesting that miR-302 can impact the response to DNA-damaging agents by modulating different target molecules. [score:5]
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Members of miR-302 family, enriched in hESCs, such as miR-302a, miR-302b, miR-302c, and miR-302d, have been shown to be directly involved in regulation of p21 expression in hESCs, demonstrating a novel function for miR-302 s in hESCs [67]. [score:5]
Tens of miRNA species were upregulated after UV-exposures, including hESC-specific miRNAs such as those of the miR-302 cluster and miR-371-372 family. [score:4]
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74
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2008.06.008 18774719 6. Subramanyam D Lamouille S Judson RL Liu JY Bucay N Derynck R Blelloch R Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cellsNat Biotechnol. [score:3]
Lipchina I Elkabetz Y Hafner M Sheridan R Mihailovic A Tuschl T Sander C Studer L Betel D Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP responseGenes Dev. [score:3]
For example, the miR-302/367 cluster (Fig.   3d and Additional file 5: Figure S3D, panel 3) is known to be abundant in hESCs in maintaining pluripotency [14]. [score:1]
miRNAs for further validation and in miR-302/367 cluster are highlighted with a black border. [score:1]
Some miRNAs known to be highly enriched in hPSCs, e. g., miR-302/367 [14], or endothelial cells, e. g., miR-126 [20], were clearly identified with. [score:1]
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75
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The miRNAs that exhibited at least a 2-fold change in expression in the hADSCs before and after the induction of chondrogenic differentiation are listed in Table I, and these include 12 upregulated miRNAs (miR-196a, miR-143, miR-383, miR-193b, let-7i, miR-26a, miR-539, miR-199a-3p, miR-337-5p, miR-146a-5p, miR-646, and miR-381) and 8 downregulated miRNAs (miR-490-5p, miR-1307, miR-125b, miR-96-3p, miR-302-3p, miR-23a-3p, miR-590, and miR-510). [score:9]
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Among 20 expressed miRNAs, the expression levels of hsa-mir-25, hsa-mir-221, hsa-mir-302b, hsa-mir-363, hsa-mir-372, hsa-mir-199a, hsa-mir-302d, hsa-mir-26a, hsa-mir-320, hsa-mir-744, hsa-mir-152 and hsa-let-7e in the study of Morin et al. exceed those obtained with miRExpress, but the levels of hsa-mir-423, hsa-let-7a, hsa-mir-1, hsa-mir-340, hsa-mir-302a, hsa-mir-130a, hsa-let-7f and hsa-mir-122 in the work by Morin et al. are lower than those obtained from miRExpress (Table 6) (full data are available in additional file 7). [score:9]
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The expression of the miR-371 cluster is downregulated before that of the miR-302/367 cluster, suggesting a temporal hierarchy in the duration of specific miRNA activity (Stadler et al., 2010; Kim et al., 2011). [score:6]
This sequence is complementary to the AAGUGC seed sequence of the miR-290-295 cluster (miR-290, miR-291a, miR-292, miR-291b, miR-294, and miR-295) and the miR-302/367 cluster (miR-302a, miR-302b, miR-302c, miR-302d, and miR-367) in mouse ESCs. [score:1]
These miRNAs include two clusters: miR-302/367 and the miR-371 cluster (miR-372 and miR-373). [score:1]
Members of the miR-302 family rescue the proliferation defects of DGCR8-mutant mouse ESCs (Wang et al., 2008) and reprogram human skin cancer cells into a pluripotent ESC-like state (Lin et al., 2008). [score:1]
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The miR-302 cluster miRNAs (miR-302a, miR-302a*, miR-302b, miR-302b*, miR-302c, miR-302c*, miR-302d) have been shown to regulate important cellular functions in hESCs, including cell proliferation and chromatin structure, and have been consistently reported to be overexpressed in hESCs [156]. [score:4]
The miRNAs overexpressed in undifferentiated hESCs like miRNA-302, 200 and 520 cluster miRNAs, were closely involved in the development of cancer. [score:4]
Some literatures have reported the relatedness between miRNA-302 family and tumorigenecity [157- 160]. [score:1]
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The expression of the miR302/367 cluster especially allows the rapid and efficient reprograming of mouse and human somatic cells to an iPSC state without forced expression of exogenous transcription factors [64]. [score:5]
In mammalian primed pluripotent stem cells, some miRNAs including miR-20, miR-92, and miR-302 regulate the apoptotic threshold and survival through targeting the pro-apoptotic protein BIM [63]. [score:4]
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80
[+] score: 8
It was found that many miRNA species are expressed predominantly in hESCs [128, 129] and genotoxic stresses, such as UV-exposures, result in differential expression of many miRNA species (e. g., miR-302 cluster, miR-371-372 family genes) [130]. [score:5]
More recently, additional miRNAs, such as miR-302 family genes, were implicated in direct regulation of the levels of p21 protein in hESCs, thus affecting the cell cycle machinery through the G1/S checkpoint that is often described as being non-operational in hESC [19]. [score:3]
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81
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Cell viability assay upon overexpression of miR-200a, miR-200b, miR-15a, miR-429 miR-302 in MDAMB-231 cells. [score:2]
S6 Fig cell proliferation assay upon overexpression of miR-200a, miR-200b, miR-15a, miR-429 and miR-302 in MDAMB-231 cells. [score:2]
S7 Fig Trypan Blue assay shows cell viability upon overexpression of miR-200a, miR-200b, miR-15a, miR-429 and miR-302 in MDAMB-231 cells. [score:2]
miR-200a, miR-200b, miR-15a, miR-429 and miR-302 reduced cell proliferation in MDAMB-231 cells. [score:1]
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The down-regulated miRNAs were highly enriched for LCL specific miRNAs (miR-155, let-7a-i, miR-21, miR-142, miR103, miR-320, miR-146a-b) and the up-regulated miRNAs were highly enriched for iPSC specific miRNAs (miR-302a, miR-302c, miR-371a, miR-302b, miR-302d, miR-372, miR-373miR-92a-1, miR-92a-2, miR-92b, miR-17, miR-20a, miR-18a). [score:7]
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Moreover, alignment of miRs that could regulate the Wnt reporter made intra- and inter -family related functional consensus sequences apparent (i. e. the seed of miR-1 and miR-613 or within the miR-302 and -515 families (Fig. S8)). [score:2]
Alignment to identify a functional consensus in the miR-515 family and its overlap with modulators the miR-302 family in regarding their ability to modulate the Wnt pathway. [score:1]
Moreover, this consensus sequence is also highly shared by the miR-activators of the miR-302 family. [score:1]
Of these, miR-302a and miR-302d are members of the miR-302 family and miR-519e, -519b, and -517a belong to the miR-515 family. [score:1]
Specifically, this correlation or trend was very clear for 6 miRs, miR-302a,-302d, -519e, -519b, -517a, and -371 e. g. ; they all hyper-activated the Wnt-reporter and happen to exhibit high sequence similarity (Fig. 2C). [score:1]
Figure S8 Extraction of a consensus sequence of identified miRs within the miR-515 and miR-302 family. [score:1]
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84
[+] score: 7
With regard to VPA treatment, the principal downregulated miRNA cluster encompassed miR-302a, b, c and d, which are well proven as markers for self-renewal and/or proliferation; consequently, VPA downregulated the pluripotency markers POU5F1, NANOG and LIN28 >7-fold (Supplementary Table S2A). [score:7]
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85
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42 members of the miR-515 family (miR-515–527) and eight members of the miR-302–367 cluster (miR-302a, miR-302a*, miR-302b, miR-302b* miR-302c, miR-302d, miR-302d* and miR-367) were only up-regulated in TGCT and not in the other two cancers, and three members of the miR-105-767 cluster (miR-105, miR-105* and miR-767-5p) were ranked as the top ten up-regulated miRNAs in TGCT. [score:7]
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86
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Lipchina I. Elkabetz Y. Hafner M. Sheridan R. Mihailovic A. Tuschl T. Sander C. Studer L. Betel D. Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP responseGenes Dev. [score:3]
Neural induction is repressed through action on the BMP/TGFβ signalling pathway, blocking the transition of stem cells towards the neural lineage, via targeting by miR-302/367, increasing BMP signalling [220]. [score:3]
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87
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We also found that miR-302∼367 cluster were up-regulated both in 4F and 3F groups, but not in the cMyc only group, indicating that miR-302∼367 were not regulated by cMyc directly. [score:6]
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88
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Four SAGE tags detected in human embryonic stem cells specifically match a cluster of four human miRNAs (mir-302a, b, c&d) known to be expressed in embryonic stem cells. [score:3]
Among the eight human miRNAs whose expression was detected by SAGE tags, four (mir-302a, b, c&d) mapped to a 600 bp region of Chr. [score:3]
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89
[+] score: 6
Intriguingly, the maintenance of precursor expression in neuronal cultures for the pluripotency -associated miR-371 and miR-520, as well as for miR-302 might indicate that these miRNAs have further functional roles beyond the switch from self-renewal to differentiation. [score:3]
This evidence is consistent with previous data showing that miR-302 is expressed in neural stem cells (e. g. [32]). [score:3]
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90
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Other miRNAs from this paper: hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-302e, hsa-mir-302f
For example, Rosa et al., showed that miR-302 family members are involved in the differentiation of human embryonic stem cells [32]; miR-302c could directly target the estrogen receptor in human breast cancer [33]. [score:4]
Dysregulation of miR-302 is seen in biliary tract cancer and thyroid cancer [34]. [score:2]
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91
[+] score: 6
Other miRNAs from this paper: hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-302e, hsa-mir-302f
To try to overcome this difficulty, some researchers are testing other possibilities to generate cancer-derived hiPSCs by the application of other factors in addition to the Yamanaka factors, such as exogenous expression of miRNA302 and chemical compounds, as azacitidine (DNA methyltransferase inhibitor) and knockdown of p53, p21, and Ink4/Arf [19, 62]. [score:6]
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92
[+] score: 6
Interestingly, CDKN1A was found to be a target for other down-regulated miRNA species we identified in our present study, namely, hsa-mir-302a, hsa-mir-302b, and hsa-mir-302d [70]. [score:6]
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93
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Instead, the miR-302 cluster was the highest expressed in human and rhesus macaque [14, 30, 31], indicating the functional divergence of stemness miRNA clusters in primate lineages. [score:3]
MiR-290-295 and miR-302 clusters are transcriptionally regulated by Oct-4/Sox2 [29]. [score:2]
The miR-290-295 cluster contains multiple mature miRNAs with seed sequences similar or identical to those in the miR-302 cluster [6]. [score:1]
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94
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Card D. A. Hebbar P. B. Li L. Trotter K. W. Komatsu Y. Mishina Y. Archer T. K. Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells Mol. [score:4]
Liu H. Deng S. Zhao Z. Zhang H. Xiao J. Song W. Gao F. Guan Y. Oct4 regulates the miR-302 cluster in P19 mouse embryonic carcinoma cells Mol. [score:2]
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95
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The genes and miRNAs expected to be enriched in iPSCs/ESCs, from the literature [18, 21, 38– 42], include transcription factors involved in maintaining “stemness” (FOXD3, GATA6, NANOG, NR6A1, POU5F1, SOX2, UTF1, TFCP2L1, and ZFP42), signaling molecules involved in pluripotency and self-renewal (CRABP2, EDNRB, FGF4, FGF5, GABRB3, GAL, GRB7, IFITM1, IL6ST, KIT, LEFTY1, LEFTY2, LIFR, NODAL, NOG, NUMB, PTEN, SFRP2, and TDGF1), cytokines and growth factors (FGF4, FGF5, LEFTY1, LEFTY2, NODAL, and TDGF1), other ESC-specific genes (BRIX1, CD9, DIAPH2, DNMT3B, IFITM2, IGF2BP2, LIN28A, PODXL, REST, SEMA3A, TERT, ESRG, and GJA1), and miRNAs (miR-302a, miR-302c, miR-371a, miR-302b, miR-302d, miR-372, miR-373, miR-92a-1, miR-92a-2, miR-92b, miR-17, miR-20a, and miR-18a) that were highly enriched in genes and miRNAs that were expressed (NRC ≥ 20) in our reprogrammed iPSCs and the majority of them showed significant upregulation (FC ≥ 2.0, FDR ≤ 0.05) during iPSC reprogramming (Figure 4(c)). [score:6]
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96
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Recently, it was discovered that ectopic expression of the miR302/367 cluster, combined with Hdac2 suppression, can directly and efficiently reprogram both mouse and human fibroblasts without supplementation with any of the Yamanaka factors (39). [score:6]
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97
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This is in agreement with the fact that high expression levels of miR-302 cluster are typical of mouse epiblast stem cells [48]. [score:3]
However, expression levels of miR-302a and miR-302b were low in rat PSCs compared to the ESC specific miR-290-295 cluster. [score:2]
This list includes homologues of human miR-302a and miR-302b. [score:1]
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98
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Subramanyam D. Lamouille S. Judson R. L. Liu J. Y. Bucay N. Derynck R. Blelloch R. Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells Nat. [score:3]
Finally, mir-302d-3p is a member of the mir-302/367 cluster that has a role in the regulation of cell signaling pathways involved in the cell cycle and inducing pluripotent stem cells [29, 30]. [score:2]
Gao Z. Zhu X. Dou Y. The miR-302/367 cluster: A comprehensive update on its evolution and functions Open Biol. [score:1]
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99
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In the context of a putative role of miRNA in pGE, it is noteworthy that several mRNAs, upregulated upon mTEC maturation, showed tissue-specific expression patterns, i. e. being restricted to brain (miR-124 and miR-129), heart (miR-499), testis (miR-202), skin (miR-203) or embryo (miR-467 and miR-302). [score:6]
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100
[+] score: 5
The qPCR (Figure  2B) confirmed strong and mouse ESC-selective expression for both miR-302 and uc. [score:3]
miR-302 was used as a positive control. [score:1]
We used ESC-specific miR-302 as a positive ncRNA control [20, 25]. [score:1]
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