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2 publications mentioning cel-mir-355

Open access articles that are associated with the species Caenorhabditis elegans and mention the gene name mir-355. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 59
DAF-2 functioned as a molecular target for mir-355 in regulating MWCNTs toxicity in nematodesThe bioinformatics analysis also suggested that daf-2 gene might serve as a molecular target for mir-355 in regulating the MWCNTs toxicity in nematodes. [score:7]
That is, mir-355 may regulate the functions of entire insulin signaling pathway by acting as an upregulator of DAF-2 in MWCNTs-exposed nematodes. [score:5]
To construct the double mutant of mir-355(n4618);daf-2(RNAi) or mir-64(nDf52);ajm-1(RNAi), RNAi was performed by feeding nematodes with E. coli strain HT115 (DE3) expressing double-stranded RNA homologous to a target gene as described previously 48 49. [score:5]
DAF-2 functioned as a molecular target for mir-355 in regulating MWCNTs toxicity in nematodes. [score:4]
In this miRNAs-mRNAs network, more interestingly, we found that daf-2 gene may act as the potential targeted gene for mir-355 in regulating the MWCNTs toxicity (Figs 4 and 7, Table S6). [score:4]
The bioinformatics analysis also suggested that daf-2 gene might serve as a molecular target for mir-355 in regulating the MWCNTs toxicity in nematodes. [score:4]
The genetic experiments have demonstrated that mir-355 could regulate the MWCNTs toxicity by suppressing the functions of daf-2 gene in nematodes (Fig. 4). [score:4]
These genetic evidences have demonstrated that daf-2 gene may be the potential targeted gene for mir-355 in regulating the MWCNTs toxicity in nematodes. [score:4]
This elucidated molecular mechanism for the function of mir-355 in regulating the MWCNTs toxicity provides an important highlight on the understanding the roles of miRNA molecules in regulating biological behavior of ENMs in organisms. [score:3]
Among the dysregulated miRNAs, the further bioinformatics analysis demonstrated that lin-4, mir-228, mir-249, mir-47, mir-355, mir-45, mir-2210, mir-57, mir-1018, mir-360, mir-64, mir-2209, mit-793, mir-1830, mir-2210, mir-83, mir-789, and mir-806 might be involved in the control of MWCNTs toxicity through affecting the functions of identified dysregulated genes in exposed nematodes (Table S6). [score:3]
Assuming that daf-2 is the potential targeted gene for mir-355, the double mutant of mir-355;daf-2 should exhibit the similar phenotypes to those in daf-2 mutant. [score:3]
In this miRNAs-mRNAs network, we provide the evidence to prove the regulation relationship between mir-355 and DAF-2/insulin receptor in regulating the MWCNTs toxicity in nematodes. [score:3]
Genetic interaction of mir-355 with DAF-2 in regulating MWCNTs toxicity in nematodes. [score:2]
Our previous study has suggested that mutation of mir-355 resulted in the susceptible property of nematodes to MWCNTs toxicity with the aid of brood size, head thrash, body bend, intestinal autofluorescence, and intestinal ROS production as the toxicity assessment endpoints 28. [score:2]
A diagram showing the mir-355 mediated signaling pathway in the control of MWCNTs toxicity in nematodes. [score:1]
The used nematode strains were wild-type N2, mir-355(n4618), daf-2(RNAi), and mir-355(n4618);daf-2(RNAi). [score:1]
C. elegans maintenanceThe used nematodes were wild-type N2, mutants of daf-16(mu86) 34, daf-2(e1370) 35, age-1(hx546) 36, daf-18(e1375) 37, akt-1(ok525) 38, isp-1(qm150) 39, mir-355(n4618) 40, mir-64(nDf52) 40, mir-249(n4983) 40, mir-355(n4618);daf-2(e1370), daf-16(mu86);daf-2(e1370), and isp-1(qm150);mir-249(n4983) and transgenic strains of Ex(P ins-7-ins-7) and zIs356[P daf-16::daf-16a/b::GFP + rol-6], which were maintained on nematode growth medium (NGM) plates seeded with Escherichia coli OP50 at 20 °C as described 10. [score:1]
As shown in Fig. 4, the double mutant of mir-355(n4618);daf-2(RNAi) exposed to MWCNTs (1 mg/L) showed the similar phenotypes of induction of intestinal ROS production, brood size, and locomotion behavior to those in daf-2(RNAi) nematodes exposed to MWCNTs (1 mg/L). [score:1]
The used nematodes were wild-type N2, mutants of daf-16(mu86) 34, daf-2(e1370) 35, age-1(hx546) 36, daf-18(e1375) 37, akt-1(ok525) 38, isp-1(qm150) 39, mir-355(n4618) 40, mir-64(nDf52) 40, mir-249(n4983) 40, mir-355(n4618);daf-2(e1370), daf-16(mu86);daf-2(e1370), and isp-1(qm150);mir-249(n4983) and transgenic strains of Ex(P ins-7-ins-7) and zIs356[P daf-16::daf-16a/b::GFP + rol-6], which were maintained on nematode growth medium (NGM) plates seeded with Escherichia coli OP50 at 20 °C as described 10. [score:1]
Considering the key and conserved functions of insulin signaling pathway in the control of many biological processes in different organisms, our work on the function of mir-355 -mediated insulin signaling pathway in the control of MWCNTs toxicity will strengthen our understanding the crucial roles and the underlying mechanism of miRNAs in the control of biological behavior of ENMs in organisms. [score:1]
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2
[+] score: 16
Other miRNAs from this paper: cel-mir-259, cel-mir-360
Therefore, on the one hand, MWCNTs may suppress the function of DAF-16 in inducing the toxicity of MWCNTs on nematodes by inhibiting the expression of mir-355; on the other hand, MWCNTs may be also able to potentially enhance the function of DAF-16 in decreasing the toxicity of MWCNTs on nematodes by increasing the expression of mir-259 (Fig. 10). [score:9]
Our recent study has demonstrated that MWCNTs exposure would inhibit the expression of mir-355, which further induced the toxic effects of MWCNTs on nematodes by suppressing the function of DAF-2 33. [score:7]
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