miRBase entry: cel-mir-355

Stem-loop cel-mir-355


Accession
MI0000754
Description
Caenorhabditis elegans cel-mir-355 precursor miRNA mir-355
Gene
family?
RF00797; mir-355

Literature search
2 open access papers mention cel-mir-355
(22 sentences)

Sequence

3914 reads, 252 reads per million, 12 experiments
aauaaugucaagaauugaauggUUUGUUUUAGCCUGAGCUAUGagucaucggaauaugcaUAGCUUCUUGCUAAAACAUGCCaaucaaucuuaauugacgaaguuuuuga
......(((((..(((((.((((.(((((((((..((((((((..((....)).....)))))).))..))))))))).)))).))))).....)))))...........

Structure
-----aauaau     ---ga     a    U         CU  -      ---ag  a 
           gucaa     auuga uggU UGUUUUAGC  GA GCUAUG     uc u
           |||||     ||||| |||| |||||||||  || ||||||     ||  
           caguu     uaacu aCCG ACAAAAUCG  CU CGAUac     ag c
aguuuuugaag     aauuc     a    U         UU  U      guaua  g 


Annotation confidence Not enough data
Do you think this miRNA is real?
Comments
This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing [1]. The 5' end of the mature sequence was mapped by PCR, and the 3' end confirmed later [2].

Genome context
chrII: 11833536-11833645 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from cel-mir-355
Name Accession Chromosome Start End Strand Confidence




Database links

Mature cel-miR-355-5p

Accession MIMAT0000697
Description Caenorhabditis elegans cel-miR-355-5p mature miRNA
Sequence 23 - UUUGUUUUAGCCUGAGCUAUG - 43
Evidence experimental
cloned [1-2], PCR [1], Illumina [3]
Database links
Predicted targets

Mature cel-miR-355-3p

Accession MIMAT0031895
Description Caenorhabditis elegans cel-miR-355-3p mature miRNA
Sequence 61 - UAGCUUCUUGCUAAAACAUGCC - 82
Evidence not_experimental

References

  1. PubMed ID: 17174894
    Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans
    "Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP"
    "Cell (2006) 127:1193-1207

  2. PubMed ID: 19460142
    Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development
    Kato M, de Lencastre A, Pincus Z, Slack FJ
    Genome Biol (2009) 10:R54

  3. PubMed ID: 15317971
    Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
    "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
    "RNA (2004) 10:1309-1322