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7 publications mentioning mmu-mir-380

Open access articles that are associated with the species Mus musculus and mention the gene name mir-380. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 157
b The seven predicted common target genes Table 2The mRNA expression level of Sp3, a suggested target gene of miR-380-3p, in the substantia nigra of Nrf2 (+/+) and Nrf2 (−/−) mice after treatment with PQ, MPTP or saline (≥2 fold, p < 0.05) Contrast Fold change p-value Regulation Nrf2 (+/+) PQ10 vs Nrf2 (−/−) PQ10 2.051.44 × 10 [−2] Up Nrf2 (−/−) MPTP30 vs Nrf2 (−/−) PQ10 3.651.69 × 10 [−2] Up Nrf2 (+/+) MPTP30 vs Nrf2 (+/+) PQ10 2.261.35 × 10 [−2] Up Nrf2 (+/+) MPTP30 vs Nrf2 (+/+) Saline 2.614.44 × 10 [−2] Up Similar to the results for the Nrf2 (+/+) mice, both MPTP treatment at 30 mg/kg and PQ treatment at 10 mg/kg decreased the expression intensity of miR-380-3p in SN of Nrf2 (−/−) ICR mice (Fig. 5b). [score:10]
In the SN of Nrf2 (+/+) mice, the expression of miR-380-3p was upregulated by low-dose PQ treatment (p < 0.01), but downregulated by high-dose PQ or MPTP treatment (p < 0.01). [score:9]
b The seven predicted common target genes Table 2The mRNA expression level of Sp3, a suggested target gene of miR-380-3p, in the substantia nigra of Nrf2 (+/+) and Nrf2 (−/−) mice after treatment with PQ, MPTP or saline (≥2 fold, p < 0.05) Contrast Fold change p-value Regulation Nrf2 (+/+) PQ10 vs Nrf2 (−/−) PQ10 2.051.44 × 10 [−2] Up Nrf2 (−/−) MPTP30 vs Nrf2 (−/−) PQ10 3.651.69 × 10 [−2] Up Nrf2 (+/+) MPTP30 vs Nrf2 (+/+) PQ10 2.261.35 × 10 [−2] Up Nrf2 (+/+) MPTP30 vs Nrf2 (+/+) Saline 2.614.44 × 10 [−2] Up MPTP-exposed mouse mo del is the most common mo del to study the mechanism of PD-related cell death because the dopaminergic neurotoxicity of MPTP can lead to DA depletion in both SN and striatum, causing behavioral change. [score:8]
b The seven predicted common target genes Table 2The mRNA expression level of Sp3, a suggested target gene of miR-380-3p, in the substantia nigra of Nrf2 (+/+) and Nrf2 (−/−) mice after treatment with PQ, MPTP or saline (≥2 fold, p < 0.05) Contrast Fold change p-value Regulation Nrf2 (+/+) PQ10 vs Nrf2 (−/−) PQ10 2.051.44 × 10 [−2] Up Nrf2 (−/−) MPTP30 vs Nrf2 (−/−) PQ10 3.651.69 × 10 [−2] Up Nrf2 (+/+) MPTP30 vs Nrf2 (+/+) PQ10 2.261.35 × 10 [−2] Up Nrf2 (+/+) MPTP30 vs Nrf2 (+/+) Saline 2.614.44 × 10 [−2] Up As compared to the control group, Nrf2 (+/+) ICR mice exposed to MPTP at 30 mg/kg exhibited neural cell damage, which was reflected by dark staining (blue–black) of cell nuclei, karyopyknosis and nucleus fragmentation (Fig. 1). [score:7]
We predicted the target genes of miR-380-3p using Miranda, Targetscan and Microcosm software which analyze the sequences of miRNA and 3'-UTR of specific genes. [score:5]
Furthermore, we predicted the mRNAs targeted by miR-380-3p using three target gene prediction programs and analyzed the possible biological effects. [score:5]
All three types of software found seven common miRNA target genes (Fig. 6a, b), among which SP3 was found to be one of the target genes of miR-380-3p (Table 2). [score:5]
The gene expression regulators Nrf2 and small regulatory RNA molecule miR-380-3p protect nerve cells from damage caused by neurotoxins. [score:5]
However, independent of Nrf2, 30 mg/kg of MPTP could cause changes in expression of miR-380-3p (difference ratio = 7.24), suggesting that miR-380-3p expression may not only depend on Nrf2. [score:5]
a Vennplot for the predictions of miR-380-3p target genes by Microcosm, Targetscan and Miranda. [score:5]
Alteration in the expression profile of microRNAs in SN tissue of Nrf2 (+/+) ICR or Nrf2 (−/−) ICR mice after treatment with PQ or MPTP was shown in Table 3. As shown in Fig. 5a, both PQ treatment at 10mg/kg and MPTP treatment at 30 mg/kg reduced the expression intensity of miR-380-3p in the SN of Nrf2 (+/+) ICR mice. [score:5]
The expression of miR-380-3p was also verified by LNA–ISH and quantitative reverse transcription PCR (QRT–PCR), which is consistent with the expression profiling. [score:5]
It can be speculated that MPTP may interact with Nrf2 in the SN, thereby regulating the expression of miR-380-3p. [score:4]
Moreover, Nrf2 deficiency prevented an upregulation of miR-380-3p following MPTP exposure. [score:4]
Nrf2 (+/+) MPTP30 (p < 0.01) Table 1The miR-380-3p expression level in the substantia nigra of Nrf2 (+/+) and Nrf2 (−/−) mice after treatment with PQ, MPTP or saline (≥1.5 fold, p < 0.05) Contrast Fold Change p-value Regulation Nrf2 (+/+) PQ10 vs. [score:4]
In fact, expression of miR-380-3p was found to be altered by the Nrf2-MPTP interaction effect (fold difference = 3.97). [score:3]
These results indicate that miR-380-3p may be involved in the neurotoxicity induced by PQ or MPTP, and that MPTP may cause changes in miRNA expression profile via interaction with Nrf2. [score:3]
LNA–ISH was performed on using LNA™ probes for miR-380-3p (Exiqon, Woburn, Mass, USA), in order to validate the results of miR-380-3p in miRNA expression profile. [score:3]
b The expression intensity of miR-380-3p analyzed by LNA–ISH hybridization in Nrf2 (−/−) ICR mice SN after saline, PQ or MPTP treatment (n = 3); each microgram (400×) shows a representative view of a slice of the SN from each group. [score:3]
Fig. 6Analysis of common target genes of miR-380-3p. [score:3]
Verification of miR-380-3p expression. [score:3]
The expression intensity of miR-380-3p in the SN of Nrf2 (−/−) ICR mice after PQ or MPTP treatment. [score:3]
a The expression intensity of miR-380-3p analyzed by LNA–ISH hybridization in the SN of Nrf2 (+/+) ICR mice after saline, PQ or MPTP treatment (n = 3); each microgram (400×) shows a representative view of a slice of the SN from each group. [score:3]
Fig. 5The expression level of miR-380-3p in the SN of Nrf2 (+/+) and Nrf2 (−/−)after treatment with PQ, MPTP or saline. [score:3]
Nrf2 (+/+) MPTP30—Nrf2 (+/+) Saline miR-140, miR-380-3p S2c The relationship between transcription factor Nrf2 and changes in miRNA expression profile after exposure to paraquat or MPTP. [score:3]
A similar result was found in the SN of Nrf2 (−/−) mice; the expression level of miR-380-3p increased at a low-dose of PQ treatment (p < 0.01) and decreased after treatment with PQ at the high-dose or MPTP (p < 0.01). [score:3]
Using real-time polymerase chain reaction (PCR), the expression of miR-380-3p in the SN in both Nrf2 (+/+) and Nrf2 (−/−) mice was confirmed. [score:3]
[Nrf2 (+/+) PQ5—Nrf2 (+/+) Saline] + [Nrf2 (+/+) PQ10—Nrf2 (+/+) Saline] miR-140, miR-380-3p S2a and 2b Changes in miRNA expression profile after exposure to MPTP. [score:3]
c The expression levels of miR-380-3p analyzed by real-time PCR in Nrf2 (+/+) and Nrf2 (−/−) SN after treatment with PQ, MPTP or saline (n = 4). [score:3]
We have found previously that expression of miR-380-3p was altered in lithium–pilocarpine -induced epilepticus mo dels of Sprague–Dawley rat and in mo dels of temporal lobe epilepsy. [score:3]
Nrf2 (−/−) MPTP30 3.974.49 ×  10 [−2] Up Similar to the results for the Nrf2 (+/+) mice, both MPTP treatment at 30 mg/kg and PQ treatment at 10 mg/kg decreased the expression intensity of miR-380-3p in SN of Nrf2 (−/−) ICR mice (Fig. 5b). [score:3]
After MPTP treatment, the expression level of miR-380-3p was higher in the SN of Nrf2 (+/+) mice than in Nrf2 (−/−) mice (p < 0.01, Fig. 5c), which is consistent with the result of microarray analysis (Table 1). [score:3]
The expression intensity of miR-380-3p in the SN of Nrf2 (+/+) ICR mice after PQ or MPTP treatment. [score:3]
Analysis of common target genes of miR-380-3p. [score:3]
Nrf2 (−/−) MPTP30 3.974.49 ×  10 [−2] Up Similar to the results for the Nrf2 (+/+) mice, both MPTP treatment at 30 mg/kg and PQ treatment at 10 mg/kg decreased the expression intensity of miR-380-3p in SN of Nrf2 (−/−) ICR mice (Fig. 5b). [score:3]
This could in turn regulate the miR-380-3p/Sp3-mRNA pathway, which is likely part of the mechanism of MPTP -induced neurodegeneration. [score:2]
A MicroRNA ISH Buffer, Controls Kit, and a LNA™ microRNA-380-3p probe were purchased from Exiqon Life Sciences, Life Sciences and Diagnostics Co. [score:1]
The arrows indicated the signal of LNA™ probes for miR-380-3p. [score:1]
[37] Current study investigating the link between Nrf2 and miR-380-3p found that expression of miR-380-3p was not altered until PQ dose rose to 10 mg/kg (fold difference = 1.95) or MPTP dose to 30 mg/kg (fold difference = 1.79). [score:1]
S7 7. Nrf2 -dependent effect of 5 mg / kg PQ Nrf2(+/+) PQ5—Nrf2(–/–) PQ5 miR-135a, miR-376c, miR-31, miR-let-7i*, miR-669b*, miR-344, miR-15b, miR-700*, miR-3099, miR-377, miR-338-5p, miR-382, miR-219-3p and miR-310a S8 8. Nrf2 -dependent effect of 10 mg / kg PQ Nrf2(+/+) PQ10—Nrf2(–/–) PQ10 miR-495*, miR-154*, miR-let-7b, miR-1983, miR-103 and miR-26a S9 The miR-380-3p / Sp3 mRNA pathway is worth to mention here. [score:1]
In our study, we selected miRNA-380-3p for computational prediction using Microcosm (www. [score:1]
Bioinformatic analysis of miRNA-380-3p and comparison. [score:1]
These findings implicate miR-380-3p in the mechanism through which Nrf2 protects the brain from Parkinson’s disease-related cell death and opens new avenues of investigation for developing more effective neuroprotective therapies. [score:1]
Therefore, the miR-380-3p/Sp3 mRNA pathway may be part of the mechanism of MPTP -induced neurotoxicity, although it requires further experimental verification. [score:1]
miR-380-3p could then alter the mRNA level of Sp3, thus affecting the biological function of the cells, inducing neurotoxicity. [score:1]
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2
[+] score: 6
Within the miR-127 cluster, no miRNA targets were identified by miRNA/mRNA pairing (Figure 4C), while all had direct mRNA targets when miRNA/protein pairs were analyzed, such as miR-380-5p, miR-370, and miR-434. [score:6]
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3
[+] score: 6
Few of the highly upregulated microRNAs were: miR-79, miR-183, miR-206, miR-207, miR-296-3p, miR-298, miR-380-5p, miR-433, miR-449b, miR-705, miR-761 (S1 Table). [score:4]
Majority of the dysregulated microRNAs e. g. miR-380, miR-207, miR-79, miR-129, miR-153, miR-183, etc. [score:2]
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4
[+] score: 5
Interestingly, 26 miRNAs including miR-337-5p and miR-380-3p were upregulated >2 fold in the mutant SMCs (Figure 3C, Table 2 and Table S2). [score:4]
miR-125a-5p, miR-29c(3p), miR-145(5p), miR-143(3p), miR-337-5p, and miR-380-3p were analyzed on the gels. [score:1]
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5
[+] score: 3
Figure 1 MicroRNAs targeting p53: miR-125b, miR-504, miR-1285, miR-92, miR-141, miR-380-5p, miR-15a, miR-16, miR-25, miR-30d, miR-200a [reviewed in Ref. [score:3]
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6
[+] score: 2
Other miRNAs from this paper: mmu-mir-155
Using a high-throughput array method, serum expression of miR-155 and miR-380 in patients who experienced cardiac death were approximately 3-4 fold higher compared with those who returned for their 1-year follow-up [12]. [score:2]
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7
[+] score: 1
Recent study has shown that a subset of circulating miRNAs (specifically, increased serum miR-155 and miR-380) are predictive for cardiac death in human patients after hospital discharge for acute myocardial infarction [31]. [score:1]
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