Stem-loop mmu-mir-380

Accession

MI0000797

Symbol

MGI: Mir380

Description

Mus musculus mmu-mir-380 precursor miRNA mir-379

Gene
family

RF04292; mir-379

Literature search

7 open access papers mention mmu-mir-380
(53 sentences)

Sequence

58234 reads, 290 reads per million, 71 experiments

aagAUGGUUGACCAUAGAACAUGCGcuacuucugugucgUAUGUAGUAUGGUCCACAUCUU
((((((...(((((((..(((((((.(((....))).)))))))..)))))))..))))))

Structure

      GUU       GA       c   u 
aagAUG   GACCAUA  ACAUGCG uac u
||||||   |||||||  ||||||| |||  
UUCUAC   CUGGUAU  UGUAUgc gug c
      -AC       GA       u   u 

Annotation confidence Medium

Comments

Seitz et al. predicted a cluster of 40 miRNAs in the imprinted human 14q32 domain, and confirmed the expression of a subset by Northern blot or primer extension in mouse, including a sequence from the 3' arm of this precursor [1]. Poy et al. cloned a sequence from the 5' arm from mouse pancreatic beta cell line MIN6 [2]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [3]. The 5' end of the miRNA may be offset with respect to previous annotations.

Genome context

chr12: 109711803-109711863 [+]

Clustered miRNAs

15 other miRNAs are < 10 kb from mmu-mir-380

Mature mmu-miR-380-5p

• Accession: MIMAT0000744
• Description: Mus musculus mmu-miR-380-5p mature miRNA
• Sequence: 4 - AUGGUUGACCAUAGAACAUGCG - 25
• Evidence: experimental ; cloned [2-3], Illumina [4-5]

Mature mmu-miR-380-3p

• Accession: MIMAT0000745
• Description: Mus musculus mmu-miR-380-3p mature miRNA
• Sequence: 40 - UAUGUAGUAUGGUCCACAUCUU - 61
• Evidence: experimental ; Northern [1], PCR [1], cloned [3], Illumina [4]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 15538371
   A pancreatic islet-specific microRNA regulates insulin secretion
   "Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M"
   "Nature (2004) 432:226-230


3. PubMed ID: 20215419
   MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
   "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
   "Mol Hum Reprod (2010) 16:463-471


4. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


5. PubMed ID: 15310658
   A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain
   "Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC, Cavaille J"
   "Genome Res (2004) 14:1741-1748