sort by

21 publications mentioning gga-mir-30c-1

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-30c-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 56
Another approach to multiple siRNA expression was stimulated by report that a mouse miR30 -based shRNA expression cassette can be driven by Pol II promoters and provide higher knockdown efficiency than those driven by the Pol III U6 promoter [10]. [score:6]
These results suggest that although NP miRNA can be expressed from the mouse miR30 -based cassette in DF-1 cells, the level of target gene knockdown is modest following stable integration of the lentiviral vector. [score:6]
Subsequently, Sun et al showed that a single Pol II promoter can drive three artificial miR30 cassettes to express siRNAs all targeting GFP, resulting in further knockdown of the GFP intensity in the cells [17]. [score:6]
The mouse miR30 -based miRNA expression cassette has been wi dely used to express artificial miRNA in lentiviral vectors [21]. [score:5]
As shown in Figure 1c, transient expression of miR30-NP inhibited Renilla luciferase activity by ∼85%. [score:5]
Inhibition of luciferase activity by NP miRNA expressed from a mouse miR30 -based lentiviral vector. [score:5]
To express anti-influenza artificial miRNA, we replaced the mature miR30 sequences in pLB2 with sequences that target nucleoprotein (NP) of influenza virus (Figure 1b). [score:5]
As a control, Vero cells were transduced with a CPGM lentivirus that expressed miR30 -based miRNA specific for the firefly luciferase transcript. [score:3]
Expression of NP miRNA from the mouse miR30 -based lentiviral vector. [score:3]
Zhou et al reported that two tandem copies of the miR30 -based cassette can be expressed in a single transcript driven by a Pol II promoter [15], [16]. [score:3]
In addition to miR30 -based designs, mouse miR155 -based design has also been used to knockdown multiple genes [19]. [score:2]
In the transient transfection assay, the miR30-NP lentiviral vector and psicheck-2 dual luciferase reporter plasmid, in which the NP target sequence was cloned into the 3′ UTR of the synthetic Renilla luciferase gene, were co -transfected into DF-1 cells. [score:2]
A similar miR30 -based approach was utilized by Zhu et al to knockdown multiple genes [18]. [score:2]
Flanking and hairpin sequences are miR30. [score:1]
0022437.g001 Figure 1(a) Schematic diagram of the miR30-NP lentiviral vector. [score:1]
Psicheck-2 dual luciferase reporter plasmid (50 ng) and miR30-NP lentiviral vector (450 ng) were co -transfected in DF-1 cells. [score:1]
[1 to 20 of 16 sentences]
2
[+] score: 26
Four up-regulated miRNAs (gga-miR-221, gga-miR-30b, gga-miR-30c and gga-miR-215) and 1 down-regulated miRNA (gga-miR-375) in RSS chicken were chosen randomly to validate the Solexa sequencing results using RT-qPCR. [score:7]
7-A, miR-30b expression profile comparison; 7-B, miR-30c expression profile comparison; 7-C, CARS mRNA expression profile comparison. [score:7]
Members of the miR-30 family function in osteoblast differentiation, adipogenic differentiation, angiogenesis, myocardial matrix remo deling and cell apoptosis by targeting various pathways [18– 22, 33]. [score:3]
6-B, comparison of miR-30c expression in livers. [score:3]
0127342.g008 Fig 8Quantitative RT-PCR assays of miR-30b and miR-30c expression using total RNA isolated from the chicken thigh muscle and hypothalamus at day1 (D1), 2, 4 and 6 w of age. [score:2]
Quantitative RT-PCR assays of miR-30b and miR-30c expression using total RNA isolated from the chicken thigh muscle and hypothalamus at day1 (D1), 2, 4 and 6 w of age. [score:2]
Gga-miR-30b and gga-miR-30c share the same seed regions and belong to the gga-miR-30 family. [score:1]
The differences of miR-30c and CARS between the two types of chickens reached a significant level at 5 or 6 w of age (Fig 6-B and 6-C) (P < 0.05). [score:1]
[1 to 20 of 8 sentences]
3
[+] score: 17
The fatty acid desaturase FADS2, which catalyses the initial desaturation step in the synthesis of long chain polyunsaturated fatty acids, was included in both the PPAR signalling pathway and the biosynthesis of unsaturated fatty acids pathway and was targeted by the down-regulated miRNA miR-30c-1-3p. [score:6]
In this study, FADS1 was targeted by miR-365-3p, miR-218-5p, miR-181a-5p, miR-181b-5p, miR-29a-3p, and miR-23b-3p, whereas FADS2 was targeted by miR-30c-1-3p. [score:5]
In addition, the putative target genes of miR-30 family members (miR-30a, -30b, -30d, -30e, and -30c) encode proteins involved in glycerophospholipid metabolism, biosynthesis of unsaturated fatty acids, pantothenate and CoA biosynthesis, and PPAR signalling pathway. [score:3]
Members of the miR-30 family are important positive regulators of adipocyte differentiation in a human adipose tissue-derived stem cell mo del 44. [score:2]
Seven conserved families all were DE with P ≤ 0.05, including let-7 (let-7a, -7b, -7c,-7f, -7g, -7i, -7j, and -7k), miR-130 (miR-130a, and -130b), miR-146 (miR-146a, -146b, and -146c), miR-15 (miR-15a, -15b, and -15c), miR-181 (miR-181a and -181b), miR-29 (miR-29a, -29b and -29c), and miR-30 (miR-30a, -30b, -30c, -30d, and -30e). [score:1]
[1 to 20 of 5 sentences]
4
[+] score: 16
Other miRNAs from this paper: hsa-let-7d, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-21, hsa-mir-22, hsa-mir-30a, hsa-mir-32, hsa-mir-33a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-147a, hsa-mir-34a, hsa-mir-187, hsa-mir-204, hsa-mir-205, hsa-mir-200b, hsa-mir-23b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-138-2, hsa-mir-142, hsa-mir-144, hsa-mir-125b-2, hsa-mir-138-1, hsa-mir-146a, hsa-mir-190a, hsa-mir-200c, hsa-mir-155, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-365b, hsa-mir-328, gga-mir-33-1, gga-mir-125b-2, gga-mir-155, gga-mir-17, gga-mir-148a, gga-mir-138-1, gga-mir-187, gga-mir-32, gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-190a, gga-mir-204-2, gga-mir-138-2, gga-let-7d, gga-let-7f, gga-mir-146a, gga-mir-205b, gga-mir-200a, gga-mir-200b, gga-mir-34a, gga-mir-30e, gga-mir-205a, gga-mir-204-1, gga-mir-23b, gga-mir-142, hsa-mir-449a, hsa-mir-489, hsa-mir-146b, hsa-mir-548a-1, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-33b, hsa-mir-449b, gga-mir-146b, gga-mir-147, gga-mir-489, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-144, gga-mir-460a, hsa-mir-147b, hsa-mir-190b, gga-mir-22, gga-mir-460b, gga-mir-1662, gga-mir-1684a, gga-mir-449c, gga-mir-146c, gga-mir-449b, gga-mir-2954, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az, gga-mir-365b, gga-mir-33-2, gga-mir-125b-1, gga-mir-190b, gga-mir-449d, gga-mir-205c
It was also demonstrated the down-regulation of miR-30c caused the up-regulated expression of platelet-derived growth factor receptor-β (PDGFRβ), and then activated the PDGF signaling which leaded to the PASMCs proliferation and phenotypes switching in a hypoxia condition [7]. [score:9]
And Caruso et al firstly reported miRNAs dysregulation in the progress of PAH by the means of microarray analysis and quantitative polymerase chain reaction, who detected that miR-451and miR-322 up regulated, whereas miR-30, miR-22, and let-7f down regulated in PAH rodent mo dels [27]. [score:4]
This research highlighted the potential role of specific miRNAs in disease progress [23], like miR-21, miR-204, miR-17, miR-155, miR-138 and miR-30c in human and rat mo dels of PAH [7, 8, 28, 29]. [score:3]
[1 to 20 of 3 sentences]
5
[+] score: 12
Of the seventeen miRNAs that are expressed at higher levels at the later stage of development (HH25) in the chicken, duck, and quail (Table S7) four belong to the miR-30 family (miR-30a-3p, miR-30a-5p, miR-30d*, and miR-30e*). [score:4]
Taken together, these miRNA changes, including differential expression of let-7, miR-302, and miR-30 families (Table S7), indicate that the HH20 to HH25 developmental window may be a critical transition phase in which multipotent NC cells begin to differentiate to form the various tissues of the face. [score:4]
Thus, up-regulation of the miR-30 family might reflect an increase in cellular differentiation at HH25. [score:4]
[1 to 20 of 3 sentences]
6
[+] score: 10
For example, a comparison of miRNA expression profiles in proliferating myoblasts and differentiated myotubes revealed that miR-221 and miR-222 are down-regulated upon differentiation of primary and established myogenic cells, whereas miR-21, miR-103, miR-130, miR-99, miR-30 and miR20 are up-regulated [19, 33], suggesting that these miRNAs play important roles in the transition between proliferation and differentiation of muscle cells. [score:9]
In addition to miR-206, miR-1 and miR-181, nine other miRNAs among the most abundant in these libraries (miR-221, miR-222, miR-21, miR-103, miR-130, miR-99, miR-30, miR20, and miR128) have been implicated in the proliferation and differentiation of muscle cells (Table 1) [15, 19, 33]. [score:1]
[1 to 20 of 2 sentences]
7
[+] score: 10
The expression level of miR-218-5p, miR-30-5p and miR-19-3p was downregulated by estrogen. [score:6]
Three miRNA clusters (miR-218-5p, miR-19-3p, and miR-30-5p) from 46 differentially expressed miRNA were found to potentially bind to the 3′UTR of ELOVL5 in the present study (Figure 2B). [score:3]
Two miRNA (miR-124a-3p and miR-124b) and four miRNA clusters (miR-19-3p, miR-218-5p, miR-124-3p, and miR-30-5p) shared by three databases were found to be potentially combine the 3′UTR of ELOVL5, respectively (Figure 2A,B and Figure S3). [score:1]
[1 to 20 of 3 sentences]
8
[+] score: 10
In additional, of the top 10 abundantly differentially expressed miRNAs identified in this study, some of them, such as miR-21 [20], miR-30c [14], miR-191 [21], let-7a and miR-22 [22] have also been reported to play important roles in the liver development. [score:4]
Moreover, we also found that miRNA-30 family and let-7 family were significantly differentially expressed between the postnatal and matured livers and were abundant in the liver, whose important roles in the liver development have been proved 14, 22. [score:4]
Hand NJ The microRNA-30 family is required for vertebrate hepatobiliary developmentGastroenterology. [score:2]
[1 to 20 of 3 sentences]
9
[+] score: 8
Other down-regulated miRNAs include members of the miR-30 family of miRNAs (gga-miR-30c, gga-miR-30d, gga-miR-30a-5p) many of which have been implicated in a wide range of cancers (Gaziel-Sovran et al., 2011; Baraniskin et al., 2012; Cheng et al., 2012). [score:4]
Other downregulated miRNAs included members of the miR-30 family of miRNAs (gga-miR-30c, gga-miR-30d, gga-miR-30a-5p) and the avian-specific gga-miR-1729 originally discovered in developing chick embryo. [score:4]
[1 to 20 of 2 sentences]
10
[+] score: 8
It has also been reported that miR-30c, let-7a, let-7b and let-7c were up-regulated, while miR-30d, miR-29a and miR-26a were down-regulated in rat granulosa cells treated with FSH for 12 h, which suggests that these miRNAs participate in FSH -mediated progesterone biosynthesis of granulosa cells [45]. [score:7]
Similarly, other miRNA families exhibited vast read ranges, such as the gga-miR146 (91–235,844 reads) and gga-miR-30 (886–164,361 reads) families (Additional file 1: Table S2). [score:1]
[1 to 20 of 2 sentences]
11
[+] score: 7
The relative expression of gga-mir-2188, gga-mir-30c-5p and gga-mir-92-3p was significantly (p < 0.01) higher in yolk than in albumen. [score:3]
The mir-30 family of miRNAs have been shown to be involved in early muscle development in zebrafish [18] and the mouse equivalent (mmu-mir-100-5p) of gga-mir-100-5p is involved in embryonic stem-cell differentiation [19]. [score:2]
Of the seven miRNAs three; gga-mir-30c-5p, gga-mir-99a-5p and gga-mir-100-5p have been reported to be present within chicken embryos [14]. [score:1]
These were gga-mir-control, as an endogenous positive control and for calculating relative expression; gga-mir-183, which was not present in either fluid, as an endogenous negative control; gga-mir-2188, gga-mir-30c-5p, gga-mir-215-5p and gga-mir-92-3p all of which were present in both fluids. [score:1]
[1 to 20 of 4 sentences]
12
[+] score: 6
3.4Wang et al. identified 32 known miRNAs from skeletal muscle of Arbor Acres commercial chickens, of which 12 form five clusters: miR-133a-1-miR-1a-2, miR-23b-miR-24, miR-99a-let-7c, miR-92-miR-19b-miR-18a-miR-17, and miR-30e-miR-30c-1, suggesting that most miRNAs co-express in skeletal muscle [85]. [score:3]
Wang et al. identified 32 known miRNAs from skeletal muscle of Arbor Acres commercial chickens, of which 12 form five clusters: miR-133a-1-miR-1a-2, miR-23b-miR-24, miR-99a-let-7c, miR-92-miR-19b-miR-18a-miR-17, and miR-30e-miR-30c-1, suggesting that most miRNAs co-express in skeletal muscle [85]. [score:3]
[1 to 20 of 2 sentences]
13
[+] score: 4
The present study of splenic miRNA and mRNA profiles from chickens after Salmonella challenge has identified differential expression of several miRNAs linked to immune responses, including miR-155, miR-9, miR-30 which have been reported previously and several miRNAs, such as miR-101-3p and miR-130b-3p, which were shown here to be associated with the immune response to infection with SE. [score:3]
Several miRNAs previously reported to be involved in immune responses such as miR-155, miR-9, miR-30, miR-126, and miR-29 families were identified. [score:1]
[1 to 20 of 2 sentences]
14
[+] score: 3
Most of the changed microRNAs are involved in development (especially in the embryonic development) and some examples of them are miRNA-222, miRNA-383, miRNA-126, miRNA-133, miRNA-30, miRNA-10a, miRNA-196 and miRNA-18b. [score:3]
[1 to 20 of 1 sentences]
15
[+] score: 2
Other miRNAs from this paper: gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-30e
Following PCR to form the siRNA with hairpin primers, the siRNA PCR fragment was then cloned into the miRNA30-like hairpin insertion site in a slx12 shuttle vector containing RFP, along with a U6 siRNA cassette containing the chicken U6 promoter and a microRNA operon expression cassette. [score:2]
[1 to 20 of 1 sentences]
16
[+] score: 2
Other miRNAs from this paper: gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-30e
Gene-specific shRNAs are embedded into the primary transcript of human miR30, which is located in the 3′UTR of a doxycycline-regulated GFP transgene. [score:2]
[1 to 20 of 1 sentences]
17
[+] score: 1
In addition to these transcripts our data list microRNAs 490-5p&3p, 1677-5p, 126-5p, 1434-5p, 193b-3p, and the members of the miR30 family, i. e., 30a-5p, 30b-5p, 30d-5p and 30e-5p, as novel cardiac enriched transcripts. [score:1]
[1 to 20 of 1 sentences]
18
[+] score: 1
A number of miRNA backbones can be used, including miR-155, miR-30 and miR17-92 [23- 25]. [score:1]
[1 to 20 of 1 sentences]
19
[+] score: 1
Further, deep sequencing detected let-7, miR-21, and miR-30 in rainbow trout eggs, which indicated that these miRNAs may play roles in controlling egg quality [21]. [score:1]
[1 to 20 of 1 sentences]
20
[+] score: 1
Other miRNAs from this paper: gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-30e
Sox14 specific oligonucleotides and a scrambled sequence were subsequently cloned in the second miRNA site of vector pRFPRNAi to generate synthetic miRNA30-like hairpins under the control of the chick U6 snRNA promoter, as described in [25]. [score:1]
[1 to 20 of 1 sentences]
21
[+] score: 1
The 20 most abundant miRNAs in the three groups were ordered by the average proportion of each miRNA and included miR-148a-3p, miR-22-3p, miR-10b-5p, miR-181a-5p, miR-133a-3p, miR-126-5p, let-7f-5p, miR-10a-5p, miR-30c-5p, miR-146c-5p (Supplementary Table 1). [score:1]
[1 to 20 of 1 sentences]