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6 publications mentioning oar-mir-432

Open access articles that are associated with the species Ovis aries and mention the gene name mir-432. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 57
Inhibition of KRT83 expression by miR-432 was confirmed by both gene knockdown with siRNA and overexpression, which was consistent with the miRNAs and targets prediction results. [score:10]
Fig. 8The overexpression of miR-432 repressed the protein expression but not mRNA expression of KRT83 in primary sheep epidemial fiber cells. [score:7]
org/vert_71/) detected a conserved miR-432 -binding site in the CDS region of KRT83 mRNA, we thus used dual luciferase reporter system to test whether miR-432 could target KRT83 expression (Fig.   7). [score:5]
** indicates p < 0.01 To further examine the effect of miR-432 on expression of KRT83 at both RNA and protein level, we transfected primary sheep epidermal fiber cells with miR-432 mimic, and then compared the expression of KRT83 in cells with that in cells treated by negative control miRNA. [score:4]
** indicates p < 0.01To further examine the effect of miR-432 on expression of KRT83 at both RNA and protein level, we transfected primary sheep epidermal fiber cells with miR-432 mimic, and then compared the expression of KRT83 in cells with that in cells treated by negative control miRNA. [score:4]
KRT83 was direct targets of miR-432. [score:4]
At the RNA level, expression of both oar-miR-432 and KRT83 was lower in adult than lamb. [score:3]
a mRNA expression of KRT83 show no significant different between cells treated by miR-432 mimic and those treated by negative control miRNA. [score:3]
Fig. 7Hela cell culture transfections showing negative relationship between oar-miR-432 and target KRT83. [score:3]
The targets sequence of the predicted miR-432 binding site that includes CDS region of KRT83 gene were cloned from sheep genomic DNA and inserted into the psi-CHECK2 plasmid (Promega) using AsiI and pmeI restriction sites downstream from the Renilla luciferase gene. [score:3]
As shown in Fig.   8, after the same normalization with ACTB as housekeeping genes, qPCR showed no significant difference in KRT83 mRNA expression between miR-432 mimic and negative control treated cells, but western blotting showed significant decrease of KRT83 protein in cells treated by miR-432 mimic compared to those treated by negative control. [score:2]
Tan sheep miRNA-mRNA analysis Curly fleece Regulatory network KRT83 miR-432 Chinese Tan sheep (Ovis aries) are indigenous to Mongolian plateau and are usually found in the Ningxia Hui Autonomous Region and Gansu province in China. [score:2]
b Wester blotting showed decreased protein expression of KRT83 in cells treated by miR-432 mimic compared to those treated by negative control miRNA. [score:2]
However, there are also some miRNA -RNA pairs with positive correlation, such as oar-miR-432 and KRT83. [score:1]
To validate binding of miR-432 with KRT83, another primer was subsequently synthesized by Sangon (Shanghai, China) to introduce mutation of some bases at the 22 bp putative binding site of miR-432 in KRT83. [score:1]
For primary sheep epidermal fiber cells, miR-432 mimic (100 nM) was transfected into cells using Lipofectamine™ 2000 (Invitrogen), according to the manufacturer’s protocol, and cells were harvested 48 h post-transfection using for RNA and protein extraction. [score:1]
Cells were transfected with two plasmids containing wild type (wt) or mutant (mut) KRT-83 CDS region, and then treated with miR-432 mimic (black) or negative control (grey). [score:1]
Plasmids containing KRT83 CDS region were co -transfected with either miR-432 or negative control miRNA to each well at a concentration of 100 nM in triplicates (Lipofectamine, Invitrogen). [score:1]
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2
[+] score: 8
Other miRNAs from this paper: mmu-mir-1a-1, mmu-mir-127, mmu-mir-134, mmu-mir-136, mmu-mir-154, mmu-mir-181a-2, mmu-mir-143, mmu-mir-196a-1, mmu-mir-196a-2, mmu-mir-21a, rno-mir-329, mmu-mir-329, mmu-mir-1a-2, mmu-mir-181a-1, mmu-mir-181b-1, mmu-mir-181c, mmu-mir-375, mmu-mir-379, mmu-mir-181b-2, rno-mir-21, rno-mir-127, rno-mir-134, rno-mir-136, rno-mir-143, rno-mir-154, rno-mir-181c, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-196a, rno-mir-181a-1, mmu-mir-196b, rno-mir-196b-1, mmu-mir-412, mmu-mir-370, oar-mir-431, oar-mir-127, oar-mir-136, mmu-mir-431, mmu-mir-433, rno-mir-431, rno-mir-433, ssc-mir-181b-2, ssc-mir-181c, ssc-mir-136, ssc-mir-196a-2, ssc-mir-21, rno-mir-370, rno-mir-412, rno-mir-1, mmu-mir-485, mmu-mir-541, rno-mir-541, rno-mir-493, rno-mir-379, rno-mir-485, mmu-mir-668, bta-mir-21, bta-mir-181a-2, bta-mir-127, bta-mir-181b-2, bta-mir-181c, mmu-mir-181d, mmu-mir-493, rno-mir-181d, rno-mir-196c, rno-mir-375, mmu-mir-1b, bta-mir-1-2, bta-mir-1-1, bta-mir-134, bta-mir-136, bta-mir-143, bta-mir-154a, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-329a, bta-mir-329b, bta-mir-370, bta-mir-375, bta-mir-379, bta-mir-412, bta-mir-431, bta-mir-432, bta-mir-433, bta-mir-485, bta-mir-493, bta-mir-541, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-1, ssc-mir-181a-1, mmu-mir-432, rno-mir-668, ssc-mir-143, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-196b-1, ssc-mir-127, ssc-mir-432, oar-mir-21, oar-mir-181a-1, oar-mir-493, oar-mir-433, oar-mir-370, oar-mir-379, oar-mir-329b, oar-mir-329a, oar-mir-134, oar-mir-668, oar-mir-485, oar-mir-154a, oar-mir-154b, oar-mir-541, oar-mir-412, mmu-mir-21b, mmu-mir-21c, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-370, ssc-mir-493, bta-mir-154c, bta-mir-154b, oar-mir-143, oar-mir-181a-2, chi-mir-1, chi-mir-127, chi-mir-134, chi-mir-136, chi-mir-143, chi-mir-154a, chi-mir-154b, chi-mir-181b, chi-mir-181c, chi-mir-181d, chi-mir-196a, chi-mir-196b, chi-mir-21, chi-mir-329a, chi-mir-329b, chi-mir-379, chi-mir-412, chi-mir-432, chi-mir-433, chi-mir-485, chi-mir-493, rno-mir-196b-2, bta-mir-668, ssc-mir-375
We speculated that the expression of mir-432 in goat skin may play a role in the development of the skin and hair follicle. [score:4]
Mir-432 is known to exhibit relatively abundant expression patterns that decrease in adulthood [24]. [score:2]
Other families that had a high abundance of reads were miR-134, miR-136, miR-154, miR-370, miR-412, miR-431, miR-432, miR-433, miR-485, miR-493, miR-541; a total of 11 miRNA families. [score:1]
These five miRNA families as well as miR-432 family have been found in these domestic animals cattle, horse, pig, sheep and goat. [score:1]
[1 to 20 of 4 sentences]
3
[+] score: 5
Other miRNAs from this paper: oar-mir-433, oar-mir-376c, oar-mir-376a, oar-mir-496, oar-mir-10b
However, three of the interactions that involved miR-1468, miR-433-3p, and miR-432 exhibited a clear direct relationship, and 12 of the 15 target genes of miR-326 had a direct relationship. [score:5]
[1 to 20 of 1 sentences]
4
[+] score: 3
Other miRNAs from this paper: oar-mir-431, oar-mir-127, oar-mir-136, oar-mir-433
Also shown are the relative positions of six conserved miRNA present in the PEG11as gene i. e. mir-431, mir-433 (consisting of mir-433-5p and mir-433-3p i. e. both stem loops produce a functional miRNA), mir-127, mir-432 and mir-136. [score:1]
PEG11as also encodes six miRNA, mir-431, mir-433 (consisting of mir-433-5p and mir-433-3p i. e. both stem loops produce a functional miRNA), mir-127, mir-432 and mir-136 [31]. [score:1]
The PEG11 open reading frame is shown in blue while the locations of antisense miRNA (mir-431, mir-433, mir-127, mir-432 and mir-136) are shown in red. [score:1]
[1 to 20 of 3 sentences]
5
[+] score: 3
The effects of two negative (NC1, NC2) and one positive (siRNA) controls are shown, as are the effects of the most inhibiting miR-329a-3p, the closely related miR-329b-3p, and the most activating miR-376a-5p and miR-432-3p. [score:3]
[1 to 20 of 1 sentences]
6
[+] score: 1
Six new sheep miRNAs were discovered in gp1a: oar-miR-127-5p, oar-miR-136-3p, oar-miR-323b-5p, oar-miR-323c-5p, oar-miR-431-3p, and oar-miR-432-3p. [score:1]
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