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21 publications mentioning dre-mir-21-2

Open access articles that are associated with the species Danio rerio and mention the gene name mir-21-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 315
The upregulation of Ptenb after miR-21 inhibition is consistent with the notion that miRNA may suppress the expression of its target mRNA. [score:12]
Alternatively, proteins with decreased expression after miR-21 knockdown may either be potential targets or may be downregulated due to indirect effects caused by miR-21. [score:10]
Of the 16 proteins, Kat7, MurC, Brd9, Tpi1b, MGC174082, Tpm1, Vaspb, G3bp2, Banf1 and Pcbp2 were upregulated at the mRNA level (Fig.   4), indicating that the inhibition effect of miR-21 was weakened due to the knockdown of miR-21, implying that these proteins are potential targets of miR-21. [score:9]
For instance, the downregulated proteins may be downstream factors of another miR-21 target, which participates in the same pathway as the miR-21 target. [score:8]
Among these DEPs, 49 were upregulated and 202 were downregulated after miR-21 knockdown. [score:8]
TMT quantitative proteomic results showed that 12 genes were downregulated and 4 genes were upregulated after miR-21 knockdown. [score:8]
However, the protein expression of Tpm1 was detected to be significantly downregulated after miR-21 suppression by both proteomics and WB. [score:8]
Among these DEPs, 49 were upregulated and 202 were downregulated after miR-21 knockdown (Table  S2). [score:8]
Another identified miR-21 target, pcbp2, was upregulated at both the mRNA and protein levels after miR-21 inhibition. [score:8]
Thus, proteins which were upregulated after miR-21 inhibition are candidate targets of miR-21. [score:8]
Several target genes of miR-21 have been identified in humans, including tumour suppressor programmed cell death 4 (PDCD4) 14, 15, phosphatase and tensin homologue tumour suppressor (PTEN) [16], tropomyosin 1 (TPM1) [17] and sprouty RTK signalling antagonist 2 (SPRY2) [18]. [score:7]
Positive and negative log2 expression ratios represent up- and down-regulation after miR-21 Knockdown respectively. [score:7]
Interestingly, TPM1 is also an miR-21 target in human cancer cells [17], implying that functional interaction of miR-21 and its targets appears to be conserved in both development and cancer biology. [score:6]
In summary, our results indicate that tpm1 and pcbp2 are direct targets of miR-21, while the other two genes may be indirectly regulated by miR-21. [score:6]
Four candidate targets of miR-21 (g3bp2, tpi1b, pcbp2 and tpm1) were selected, and luciferase assays were performed to determine whether miR-21 directly regulates these potential targets. [score:6]
Thus, miR-21 may modulate the expression of critical genes in valvulogenesis or other developmental processes by targeting Pcbp2. [score:6]
In zebrafish, miR-21 is specifically expressed in the heart and brain and has been shown to be a critical regulator of cardiac valvulogenesis during embryonic development 7, 26. [score:5]
In the present study, we carried out a loss-of-function experiment by inhibiting the expression of miR-21 in zebrafish embryos. [score:5]
The potential targets of miR-21 were predicted using the algorithms of TargetScanFish (http://www. [score:5]
In mammals, miR-21 is reported to be highly expressed in malignant tissues and thought to play important roles in tumour invasion 8, 9. miR-21 expression is also detected during cardiac remo delling, and it has been implicated in heart failure and several forms of cardiac stress 10– 13. [score:5]
Using a Tandem Isobaric Mass Tag (TMT) -based quantitative proteomic strategy, we found that 251 out of 2675 proteins quantitated were differentially expressed after miR-21 inhibition. [score:5]
Our results show that miR-21 can regulate numerous cellular pathways by modulating the expression of hundreds of proteins. [score:4]
Western blot analysis of protein expression levels of four representative proteins quantified by proteomics after miR-21 knockdown. [score:4]
MOs targeting miR-21-2 was used in the miR-21 knockdown in quantitative proteomic strategy. [score:4]
Thus, it is essential to identify miR-21-regulated targets at the protein level. [score:4]
It plays critical roles in valvulogenesis by regulating the same targets as those of the human/mouse miR-21 (pdcd4, sprouty and ptenb) [7]. [score:4]
Additional miR-21 targets should be identified with the same approach in subsequent studies, allowing a better understanding of the molecular basis of miR-21 -mediated regulation of valvulogenesis. [score:4]
Hundreds of proteins which are involved in various aspects of cellular processes were dysregulated after miR-21 inhibition, suggesting that miR-21 is multi-functional. [score:4]
Injection of miR-21 MOs knocked down the expression of miR-21 to nearly 30%. [score:4]
Using this approach, we determined that 251 proteins were differentially regulated after miR-21 inhibition (log2 fold change ≥0.27 or ≤−0.27, CV < 30%). [score:4]
The GO classification and pathway analysis revealed that miR-21 may regulate various aspects of the cellular function (Fig.   3), and the miR-21-regulated proteins participate in many different pathways (Table  S3). [score:3]
Sixteen proteins were predicted to be miR-21 targets in zebrafish by at least two types of software (Table  S4). [score:3]
The mRNAs of 16 DEPs were also predicted to be miR-21 targets by at least two algorithms (Table  S4). [score:3]
Two genes, tpm1 and pcbp2, were verified to be novel targets of miR-21. [score:3]
Six different algorithms were employed to predict the targets of miR-21 as described above. [score:3]
In zebrafish, the cardiac expression of miR-21 was first detected at 48 hours post fertilization (hpf), and was restricted to the atrioventricular valve (AV) ring endocardium [26]. [score:3]
Validation of candidate target genes of miR-21. [score:3]
We further confirmed that tropomyosin 1 (tpm1) and poly(rC) binding protein 2 (pcbp2) identified from a proteomic profiling are direct targets of miR-21 using luciferase assays. [score:3]
To identify potential miR-21 target genes, we performed TMT -based quantitative proteomic analysis as summarized in Fig.   2A. [score:3]
The systematic identification of miR-21-regulated proteins provides a valuable source for further in-depth studies of the exact regulatory mechanisms of miR-21 in cardiac valvulogenesis. [score:3]
However, injection of only 4 ng of a MO targeting pre-miR-21-2 (miR-21-2, multi-blocking) resulted in incomplete looping of the heart tube in 80% (100/120) of the embryos (Fig.   1). [score:3]
Figure 1The effect of miR-21 knockdown on the zebrafish valve development. [score:3]
Figure 6Validation of candidate miR-21 target genes. [score:3]
In zebrafish, miR-21 is specifically expressed in the heart and brain, and it seems to be confined to the valvular endothelium within the heart [3]. [score:3]
Intriguingly, of the three validated miR-21 targets, Spry2 and Ptenb are also associated with these DEPs. [score:3]
Then, miR-21 expression was observed in the cardiac outflow tract and the developing central nervous system by 72 hpf [26]. [score:3]
miR-21 Target Prediction. [score:3]
A PPI network was generated using the 251 DEPs and the 3 reported miR-21 targets. [score:3]
These genes are potential miR-21 targets. [score:3]
In a previous study, we successfully identified miR-21 targets in human cancer cells by employing a Stable Isotope Labelling by Amino acids in Cell culture (SILAC) -based quantitative proteomic approach [25]. [score:3]
In the present study, global proteomic profiling was carried out to identify targets of miR-21 in zebrafish embryos. [score:3]
However, only a few miR-21 targets have been validated, and their functional mechanisms are largely unknown. [score:3]
Several target genes of miR-21 have previously been identified in humans, including pdcd4 14, 15, pten [16], tpm1 [17], spry2 [18] and stat3 [25]. [score:3]
The DEPs also interacted with validated miR-21 targets in the zebrafish (Fig.   S3). [score:3]
Several DEPs of biological interest (nanog homeobox (Nanog), Pcbp2, Tpm1 and Histone acetyltransferase (Kat7)) from the 251 proteins dysregulated after miR-21 knockdown were further validated by WB (Fig.   5). [score:3]
Two hundred and fifty-one proteins with a log2 fold change ≥0.27 or ≤−0.27 (CV < 30%) were considered to be dysregulated after miR-21 inhibition (Table  S2). [score:3]
miR-21 is highly conserved between humans and zebrafish, pdcd4, spry2 and ptenb are also confirmed targets of miR-21 in zebrafish 7, 26. [score:3]
A PPI network of the DEPs and two reported miR-21 targets (pdcd4 and spry2) were generated using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database (version 10.0, http://www. [score:3]
Taken together, these results imply that miR-21 may be an important regulator of diverse cellular functions across widespread biological processes during zebrafish embryonic development. [score:3]
For the predicted miR-21 target genes, total RNA was reverse transcribed into first-strand cDNA using cDNA reverse transcription kit (Invitrogen). [score:3]
The results showed that overexpression of miR-21 significantly decreased the luciferase activity of the reporter vector containing the 3′-UTRs of tpm1 and pcbp2 (Fig.   6). [score:3]
Effect of miR-21 Knockdown. [score:2]
GO distribution of miR-21- regulated proteins according to their biological process, molecular function and cellular component categories. [score:2]
Hundreds of proteins have been identified to be miR-21-regulated proteins in zebrafish embryos, which are attributed to the priority of the high-throughput MS analysis. [score:2]
of miR-21-regulated Proteins. [score:2]
Two recent studies revealed that miR-21 is also crucial in the regulation of cardiac valvulogenesis 7, 8. miR-21 is conserved between humans and zebrafish, with only two base pair differences in their mature forms. [score:2]
Figure 2Quantitative proteomic identification of miR-21-regulated proteins in zebrafish embryos. [score:2]
Then, we performed a TMT -based quantitative proteomic analysis together with bioinformatics and luciferase reporter assays to identify the protein targets of miR-21 in zebrafish embryos. [score:2]
Screening of Potential miR-21-regulated Proteins Using a Quantitative Proteomic Strategy. [score:2]
The results showed that knocking down miR-21 resulted in a failure of cardiac looping, which is consistent with previous studies 7, 26. [score:2]
The TaqMan small RNA Assays System was used to detect the expression of mature miR-21 as previously described [26]. [score:2]
The majority of the quantitated proteins had a log2 fold change (miR-21-knockdown group (KD)/negative control group (NC)) between −0.27 and 0.27 (Fig.   S2). [score:2]
Figure 3 GO classification of miR-21-regulated proteins. [score:2]
The constructs were co -transfected with miR-21 mimics into HEK293T cells. [score:1]
In zebrafish, miR-21 is critical in heart valve formation. [score:1]
Significantly decreased luciferase activity was detected in 293 T cells co -transfected with miR-21 mimics and psi-CHECK2- tpm1-WT or psi-CHECK2- pcbp2-WT (Fig.   6B). [score:1]
HEK293T cells were transfected with psi-CHECK2 vectors harbouring the wild type or mutated miR-21 -binding site and the miR-21 mimics. [score:1]
To gain insights into the biological functions of miR-21, we first classified the DEPs using Protein Analysis Through Evolutionary Relationships (PANTHER) Protein Class ontology (http://www. [score:1]
In brief, 10 μg protein extracted from the embryos of both the miR-21 KD and NC groups were separated by 12% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Waukesha, WI, USA). [score:1]
Moreover, when the sites complementary to the seed region in the 3′-UTR of miR-21 were mutated, the luciferase activities of these four genes were not affected (Fig.   6). [score:1]
Two genomic copies of miR- 21 exist in zebrafish, which can generate the same mature miRNA (miR-21-1 and miR-21-2). [score:1]
However, the PPI information will allow us to better understand the functional mechanisms of miR-21. [score:1]
By comparison, no significant changes were observed in the luciferase activity in cells co -transfected with the psi-CHECK2 vectors harbouring the 3′-UTR of the other two genes and the miR-21 mimics. [score:1]
The mutated plasmids psi-CHECK2- g3bp2-Mut (the TAAGC sequence in the complementary site for the seed region of miR-21 was mutated to GCCUA), psi-CHECK2- tpi1b-Mut (TAAGC to GCCUA), psi-CHECK2- pcbp2-Mut (AAGC to CCUA) and psi-CHECK2- tpm1-Mut (AAGC to CCUA) were generated from the psi-CHECK2 vector containing the wild type 3′-UTR using a QuickChange kit (Stratagene, La Jolla, CA, USA). [score:1]
This study is the first to explore the function of miR-21 in zebrafish embryos using a quantitative proteomic approach. [score:1]
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2
[+] score: 168
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7e, hsa-mir-20a, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-31, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-203a, hsa-mir-204, hsa-mir-212, hsa-mir-181a-1, hsa-mir-221, hsa-mir-23b, hsa-mir-27b, hsa-mir-128-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-143, hsa-mir-200c, hsa-mir-181b-2, hsa-mir-128-2, hsa-mir-200a, hsa-mir-30e, hsa-mir-148b, hsa-mir-338, hsa-mir-133b, dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-10b-1, dre-mir-181b-1, dre-mir-181b-2, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-203a, dre-mir-204-1, dre-mir-181a-1, dre-mir-221, dre-mir-222a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7e, dre-mir-7a-3, dre-mir-10b-2, dre-mir-20a, dre-mir-21-1, dre-mir-23a-1, dre-mir-23a-2, dre-mir-23a-3, dre-mir-23b, dre-mir-24-4, dre-mir-24-2, dre-mir-24-3, dre-mir-24-1, dre-mir-26b, dre-mir-27a, dre-mir-27b, dre-mir-29b-1, dre-mir-29b-2, dre-mir-29a, dre-mir-30e-2, dre-mir-101b, dre-mir-103, dre-mir-128-1, dre-mir-128-2, dre-mir-132-1, dre-mir-132-2, dre-mir-133a-2, dre-mir-133a-1, dre-mir-133b, dre-mir-133c, dre-mir-143, dre-mir-148, dre-mir-181c, dre-mir-200a, dre-mir-200c, dre-mir-203b, dre-mir-204-2, dre-mir-338-1, dre-mir-338-2, dre-mir-454b, hsa-mir-181d, dre-mir-212, dre-mir-181a-2, hsa-mir-551a, hsa-mir-551b, dre-mir-31, dre-mir-722, dre-mir-724, dre-mir-725, dre-mir-735, dre-mir-740, hsa-mir-103b-1, hsa-mir-103b-2, dre-mir-2184, hsa-mir-203b, dre-mir-7146, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-181b-3, dre-mir-181d, dre-mir-204-3, dre-mir-24b, dre-mir-7133, dre-mir-128-3, dre-mir-7132, dre-mir-338-3
As miR-21 was the most highly upregulated and most highly expressed miRNA in all three mo dels, we validated the expression of known target genes, including the tumor suppressor, pdcd4, and TGFβ receptor subunit, tgfbr2 and novel putative target genes such as the anti-apoptotic factor, bcl2l13, Choline kinase alpha, chka and the regulator of G-protein signaling, rgs5. [score:15]
As miR-21 was the most highly upregulated and most highly expressed miRNA in all three mo dels, we validated the expression of known target genes, including the tumor suppressor, pdcd4, and TGFβ receptor subunit, tgfbr2 [29, 30] and novel putative targets such as the anti-apoptotic factor, bcl2l13, the Choline Kinase Alpha, chka and the Regulator of G-protein signaling, rgs5. [score:15]
Upregulation of miR-21, miR-31 and miR-181c leads to the downregulation of inhibitors and suppressors such as pdcd4, tgfbr2, bcl2l13, rgs5 and chka, downregulated genes with anti-proliferative functions. [score:14]
Fig 7 summarizes the gene regulatory circuit for miR-21, miR-31 and miR-181c, the 3 validated, shared upregulated miRNAs, with downregulated putative target genes that have functional relationships with conserved blastema -associated genes. [score:10]
Among the differentially upregulated miRNAs across all three regenerating systems, miR-21 was the most highly expressed and upregulated miRNA (Figs 2C and 3A). [score:9]
Following injury, three miRNAs (miR-21, miR-31 and miR-181c) are commonly upregulated and target a set of five commonly downregulated genes shown with blue lines. [score:9]
0157106.g007 Fig 7Following injury, three miRNAs (miR-21, miR-31 and miR-181c) are commonly upregulated and target a set of five commonly downregulated genes shown with blue lines. [score:9]
These filtering criteria identified 136 downregulated genes with predicted binding sites in the 3’-UTRs for any of the 5 common upregulated miRNAs (miR-21, miR-31, miR-181b, miR-181c and miR-7b) (S21 Table). [score:7]
These studies confirmed miR-21, miR-181c and miR-31 were consistently upregulated in all three organisms and miR-181b and miR-7b were upregulated in both zebrafish and bichir (Fig 3). [score:7]
S22 Table Zebrafish Ensembl gene identifiers for 58 genes downregulated in three mo dels with predicted miRNA binding sites for miR-21, miR-181c, miR-181b, miR-31 and miR-7 and members of the network of commonly up- and downregulated genes with functional interactions to 11 blastema -associated genes. [score:7]
miR-21 was the most abundantly expressed miRNA and the most highly upregulated in all three systems (Fig 2C and S2– S4 Tables). [score:6]
As miR-21 is the most highly upregulated and most highly expressed miRNA in all three mo dels, we further investigated miR-21 target genes. [score:6]
The other three genes, bcl2l13, rgs5 and chka, were selected because we predicted them to be targeted by both miR-21 and miR-181c and they have inhibitory roles in cellular proliferation. [score:5]
0157106.g006 Fig 6 (a) Network of blastema -associated genes, miR-21 and predicted miR-21 targets differentially expressed in three mo del systems. [score:5]
Morphological and histological studies of miR-21, miR-31 and/or miR-181 inhibition combined with identification of target genes would demonstrate their roles in blastema formation. [score:5]
This analysis identified 2 known miR-21 target genes (pdcd4 and tgfbr2) and 3 putative novel targets (bcl2l13, rgs5 and chka) that are implicated during blastema formation. [score:5]
Within this subset of differentially regulated zebrafish miRNAs, we identified 10 miRNAs: miR-21, miR-181c, miR-181b, miR-31, miR-7b, miR-2184, miR-24, miR-133a, miR-338 and miR-204, that showed conserved expression changes with both bichir and axolotl regenerating samples (Table 1). [score:4]
We identified a subset of 19 downregulated transcripts from the 58 blastema -associated genes with a predicted miR-21 binding site (Fig 6A and S2 File). [score:4]
S21 Table Zebrafish Ensembl gene identifiers for 136 genes downregulated in three mo dels with predicted miRNA binding sites for miR-21, miR-181c, miR-181b, miR-31 or miR-7 in all three mo dels. [score:4]
Previous profiling studies have also identified miR-21 as a highly upregulated miRNA in response to injury in multiple tissues and numerous organisms [2, 27, 41, 69]. [score:4]
Both pdcd4 and tgfbr2 were chosen for validation because they are known miR-21 targets. [score:3]
STRING interactions with 11 common blastema -associated genes, miR-21, miR-31, miR-181, and 50 additional common differentially expressed genes with common predicted miRNAs binding sites. [score:3]
Next, we established a gene network for common miRNA target genes for miR-21, miR-31 and miR-181. [score:3]
Predicted miR-21 binding sites predicted by miRanda [75] within 3’-UTRs for zebrafish, bichir and axolotl target genes. [score:3]
miR-21 regulation of blastema -associated genes. [score:2]
Predicted miR-21 binding sites for genes shown in Fig 6A. [score:1]
Defining miR-21 mechanism of action in these different contexts thus provide an important opportunity with which to define the cellular and molecular mechanisms of endogenous repair and regeneration processes that have been conserved during evolution. [score:1]
S2 FilePredicted miR-21 binding sites for genes shown in Fig 6A. [score:1]
In addition to rgs5, both bcl2l13 and chka had predicted binding sites for 4 miRNAs (miR-21, miR-181b, miR-181c and miR-7b). [score:1]
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3
[+] score: 28
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-18a, hsa-mir-21, hsa-mir-27a, hsa-mir-96, hsa-mir-99a, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-30b, mmu-mir-99a, mmu-mir-124-3, mmu-mir-125b-2, mmu-mir-9-2, mmu-mir-135a-1, mmu-mir-181a-2, mmu-mir-182, mmu-mir-183, mmu-mir-199a-1, hsa-mir-199a-1, mmu-mir-200b, hsa-mir-181a-2, hsa-mir-182, hsa-mir-183, hsa-mir-199a-2, hsa-mir-181a-1, hsa-mir-200b, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-125b-1, hsa-mir-135a-1, hsa-mir-135a-2, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-18a, mmu-mir-21a, mmu-mir-27a, mmu-mir-96, mmu-mir-135b, mmu-mir-181a-1, mmu-mir-199a-2, mmu-mir-135a-2, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-125b-1, hsa-mir-200a, hsa-mir-135b, dre-mir-182, dre-mir-183, dre-mir-181a-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-15a-1, dre-mir-15a-2, dre-mir-18a, dre-mir-21-1, dre-mir-27a, dre-mir-27b, dre-mir-27c, dre-mir-27d, dre-mir-27e, dre-mir-30b, dre-mir-96, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-125b-1, dre-mir-125b-2, dre-mir-125b-3, dre-mir-135c-1, dre-mir-135c-2, dre-mir-200a, dre-mir-200b, dre-let-7j, dre-mir-135b, dre-mir-181a-2, dre-mir-135a, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, mmu-let-7k, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3
A regulatory mo del was suggested, based on known miR-21 targets, involving infection as the first stimulation, followed by factors that activate miR-21 transcription, which then suppresses PTEN and PDCD-4 (known tumour suppressor genes) and leads to cholesteatoma growth. [score:8]
A study examining cholesteatomas revealed upregulation of miR-21, leading to a mo del explaining the potential growth of this benign yet potentially harmful growth in the middle ear or the mastoid bone (Friedland et al, 2009). [score:4]
From a set of suspected miRNAs previously linked to proliferation and growth in other mo dels, miR-21 was found to be up-regulated in cholesteatoma samples, as compared to normal skin. [score:3]
PTEN, a known miR-21 target, was found to decrease in protein levels, but not in the mRNA levels of VS samples. [score:3]
miRNAs were also described in other ear related diseases such as the role of miR-21 in human cholesteatoma growth and proliferation (Friedland et al, 2009) and in vestibular schwannomas (VS; Cioffi et al, 2010). [score:3]
miR-21 was knocked down in primary VS cultures using anti-miR-21 oligonucleotides, which increased cell proliferation and survival. [score:2]
In a different study, miR-21 was found to be over-expressed in VS nerve tissue, as compared to a normal tissue (Cioffi et al, 2010; Fig 3). [score:2]
One of the studies discovered a regulatory role of miR-21 in human cholesteatoma growth and proliferation (Friedland et al, 2009; Fig 3). [score:2]
Vestibular schwannomas have also been found to contain increased levels of miR-21 (Cioffi et al, 2010). [score:1]
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4
[+] score: 26
Two predicted targets of miR-21 are NDRG1B and NDRG4, members of the N-myc downstream regulated gene (NDRG) family, which are downregulated in Tbx5 morphants at 48 hpf. [score:7]
Another interesting miRNA that we found upregulated at 48 hpf is miR-21 whose deregulation in heart has been reported to contribute to cardiovascular disease (Jazbutyte and Thum, 2010). [score:7]
We selected the most variable miRNAs, in terms of expression fold-change between Tbx5 and Ct morphants for downstream analysis: miR-34a, miR-10d-5p, miR-30a, miR-210-3p, and miR-5p at 24 hpf, miR-34a, miR-462, miR-146a, miR-21, miR-7b, and miR-190b at 48 hpf (Table 1). [score:3]
Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow -dependent expression of mir-21. [score:3]
Therefore, we hypothesized that Tbx5 might affect NDRG4 expression through miR-21 modulation. [score:3]
MicroRNA-21: from cancer to cardiovascular disease. [score:2]
stage miRNA FC p-val adj p-val 24 hpf dre-miR-34a 2.82 1.03e−12 2.99e−10 dre-miR-10d-5p 0.55 11.24e−07 6.77e−06 dre-miR-30a 0.41 9.40e−12 1.02e−09 dre-miR-210-3p 0.33 8.29e−12 1.02e−09 dre-miR-210-5p 0.26 1.68e−10 1.22e−08 48 hpf dre-miR-34a 6.62 7.43e−16 2.70e−14 dre-miR-462 5.6 5.95e−10 7.63e−09 dre-miR-146a 4.51 1.05e−09 1e27e−08 dre-miR-21 2.84 1.65e−10 2.25e−09dre-miR-19a-3p [a] 0.68 8.52e−02 4.91e−02 dre-miR-7b 0.10 1.83e−07 1. 66e−06 dre-miR-190b 0.01 1.21e−18 5.29e−17 [a]Data for miR-19a-3p comes from our previous published data in (Chiavacci et al., 2015). [score:1]
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5
[+] score: 17
Together with qRT-PCR results confirming that miR-23a and miR-21 were indeed overexpressed in zebrafish via vector -based miRNA expression assay (Figure 1C), we found out that miR-23a, a miRNA that has not been reported to be associated with axon regeneration, had no obvious effect on M-cell axon regeneration; while miR-21, which has been shown to promote regeneration in different organs (Strickland et al., 2011; Han et al., 2014; Hoppe et al., 2015), remarkably promoted M-cell axon regeneration (control: 243.7 ± 32.9 μm, n = 33 fish vs. [score:4]
To overexpress miRNAs, a construct containing pri-miR-133b/pri-miR-23a/pri-miR-21 was made by amplifying a genomic region containing the miR-133b/miR-23a/miR-21 precursor. [score:3]
To further verify the specific role of miR-133b in regulating axon regeneration, we overexpressed another two miRNAs, miR-23a and miR-21, with the same assay as mentioned above. [score:3]
With another two miRNAs (miR-23a and miR-21) having different effects on axon regeneration, we reported that overexpression of miR-133b specifically reduced the regenerative length in M-cell (Figure 7). [score:3]
miR-21 OE: 778.4 ± 60.8 μm, n = 12 fish; Figures 1E,F). [score:1]
miR-21 alleviated apoptosis of cortical neurons through promoting PTEN-Akt signaling pathway in vitro after experimental traumatic brain injury. [score:1]
Axotomy -induced miR-21 promotes axon growth in adult dorsal root ganglion neurons. [score:1]
miR-21 OE, P < 0.0001; control vs. [score:1]
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6
[+] score: 16
In particular, miR-29, which is down-regulated in response to cardiac injury, has been shown to inhibit the expression of fibrotic genes [148], while miR-21, which is upregulated in response to cardiac stress, has been proposed to promote it [149, 150], although a miR-21 KO mouse mo del raises questions on the essential nature of this response [151]. [score:11]
Roy S. Khanna S. Hussain S. R. A. Biswas S. Azad A. Rink C. Gnyawali S. Shilo S. Nuovo G. J. Sen C. K. MicroRNA expression in response to murine myocardial infarction: miR-21 regulates fibroblast metalloprotease-2 via phosphatase and tensin homologue Cardiovasc. [score:4]
Patrick D. M. Montgomery R. L. Qi X. Obad S. Kauppinen S. Hill J. A. van Rooij E. Olson E. N. Stress -dependent cardiac remo deling occurs in the absence of microRNA-21 in mice J. Clin. [score:1]
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7
[+] score: 16
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-21, hsa-mir-29a, hsa-mir-96, mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-140, mmu-mir-181a-2, mmu-mir-182, mmu-mir-183, mmu-mir-194-1, mmu-mir-200b, hsa-mir-34a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-183, hsa-mir-181a-1, hsa-mir-200b, mmu-mir-34c, mmu-mir-34b, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-140, hsa-mir-194-1, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-21a, mmu-mir-29a, mmu-mir-96, mmu-mir-34a, mmu-mir-135b, hsa-mir-200c, hsa-mir-181b-2, mmu-mir-17, mmu-mir-200c, mmu-mir-181a-1, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-181b-1, mmu-mir-181c, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-34b, hsa-mir-34c, hsa-mir-376c, hsa-mir-376a-1, mmu-mir-376a, hsa-mir-135b, mmu-mir-181b-2, mmu-mir-376b, dre-mir-34a, dre-mir-181b-1, dre-mir-181b-2, dre-mir-182, dre-mir-183, dre-mir-181a-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-15a-1, dre-mir-15a-2, dre-mir-17a-1, dre-mir-17a-2, dre-mir-21-1, dre-mir-29a, dre-mir-96, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-140, dre-mir-181c, dre-mir-194a, dre-mir-194b, dre-mir-200b, dre-mir-200c, hsa-mir-376b, hsa-mir-181d, hsa-mir-507, dre-let-7j, dre-mir-135b, dre-mir-181a-2, hsa-mir-376a-2, mmu-mir-376c, dre-mir-34b, dre-mir-34c, mmu-mir-181d, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, mmu-let-7k, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-181b-3, dre-mir-181d, mmu-mir-124b
Friedman et al. (2009) miR-21 Phosphatase and tensin homolog (Pten) Down-regulation of target in cholesteatoma; inhibition of miR. [score:8]
Skin samples from cholesteatoma patients and control individuals were analyzed for protein levels of the putative miR-21 targets, PTEN, and PCDC4 (Friedland et al., 2009). [score:3]
Cholesteatoma growth and proliferation: posttranscriptional regulation by microRNA-21. [score:2]
MicroRNA-21 overexpression contributes to vestibular schwannoma cell proliferation and survival. [score:2]
Friedland et al. (2009), Cioffi et al. (2010) miR-21 Programmed cell death 4 (Pdcd4) Western blot on cholesteatoma skin samples. [score:1]
[1 to 20 of 5 sentences]
8
[+] score: 13
The gene pdcd4, encoding programmed cell death protein 4, was found to be an important in vivo target of miRNA-21, and inhibition of miRNA-21 binding to pdcd4 resulted in failure of atrioventricular valve development (Kolpa et al., 2013). [score:6]
Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow -dependent expression of miR-21. [score:3]
Similarly, miRNA-21 is required for normal valvulogenesis in the zebrafish, and its expression is highly dependent on blood flow (Banjo et al., 2013). [score:3]
miR-21 represses Pdcd4 during cardiac valvulogenesis. [score:1]
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9
[+] score: 9
Several of these miRNAs were commonly up-regulated by both of the infection conditions, including miR-21 (mature miRNA and its star sequence), miR-29a, miR-29b, miR-146a, and miR-146b (Figure 1A). [score:4]
By microarray analysis of miRNA expression in zebrafish we found that miRNAs of the miR-21, miR-29, and miR-146 families were commonly induced by infection of embryos with S. typhimurium and by infection of adult fish with M. marinum. [score:3]
The miR-146 family members were commonly induced during infections of embryos and adult fish, along with miRNAs of the miR-21 and miR-29 families, which also have been implicated in immunity and infection. [score:1]
The induction of members of the miR-21, miR-29, and miR-146 families was in line with earlier microarray studies, which reported these along with some other miRNAs, like miR-9, miR-132, miR-147, and miR-155 as infection-inducible [13, 26, 43, 44]. [score:1]
[1 to 20 of 4 sentences]
10
[+] score: 8
Other miRNAs from this paper: dre-mir-10a, dre-mir-10b-1, dre-mir-204-1, dre-mir-181a-1, dre-mir-214, dre-mir-222a, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-10b-2, dre-mir-10c, dre-mir-10d, dre-mir-17a-1, dre-mir-17a-2, dre-mir-21-1, dre-mir-22a, dre-mir-22b, dre-mir-25, dre-mir-26a-1, dre-mir-26a-2, dre-mir-26a-3, dre-mir-30d, dre-mir-92a-1, dre-mir-92a-2, dre-mir-92b, dre-mir-100-1, dre-mir-100-2, dre-mir-125a-1, dre-mir-125a-2, dre-mir-125b-1, dre-mir-125b-2, dre-mir-125b-3, dre-mir-125c, dre-mir-126a, dre-mir-143, dre-mir-146a, dre-mir-462, dre-mir-202, dre-mir-204-2, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, dre-mir-181a-2, dre-mir-1388, dre-mir-222b, dre-mir-126b, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-204-3
The most enriched GO term was segment polarity determination (GO:0007367), which contained target mRNA for the miR-10 and miR-125 families, as well as miR-181a-5p miR-21-5p, miR-222a-3p, and miR-430b-3p. [score:3]
The most frequently abundant was miR-143-3p, which was among the 10 most abundant miRNAs in both testis and ovary in all five species examined, followed by miR-21-3p, which was abundant in all except Nile tilapia. [score:1]
In addition, miR-10b-5p, miR-125a-5p, miR-143–3p, miR-181a-5p, and miR-21–5p were among the most abundant miRNAs in at least six of nine gonadal stages (Fig. 4b). [score:1]
let-7a-1-5p, let-7c-5p and miR-92b-3p were more abundant in spermatozoa only, whereas miR-430b-3p and miR-21-5p were more abundant in oocytes only. [score:1]
Among the most frequently abundant miRNAs between species were let-7a-5p, miR-143-3p, miR-181a-5p, miR-202-5p, and miR-21-5p (Supplementary Fig. S2B). [score:1]
miR-21-5p was the only miRNA which was among the most abundant miRNA in both unfertilized eggs and the ovary (Fig. 4a,c). [score:1]
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[+] score: 6
Other miRNAs from this paper: dre-mir-196a-1, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-203a, dre-mir-210, dre-mir-214, dre-mir-219-1, dre-mir-219-2, dre-mir-221, dre-mir-222a, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-mir-429a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-1-2, dre-mir-1-1, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-21-1, dre-mir-25, dre-mir-30e-2, dre-mir-101a, dre-mir-103, dre-mir-107a, dre-mir-122, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-126a, dre-mir-129-2, dre-mir-129-1, dre-mir-130b, dre-mir-130c-1, dre-mir-130c-2, dre-mir-133a-2, dre-mir-133a-1, dre-mir-133b, dre-mir-133c, dre-mir-135c-1, dre-mir-135c-2, dre-mir-140, dre-mir-142a, dre-mir-142b, dre-mir-150, dre-mir-152, dre-mir-462, dre-mir-196a-2, dre-mir-196b, dre-mir-202, dre-mir-203b, dre-mir-219-3, dre-mir-365-1, dre-mir-365-2, dre-mir-365-3, dre-mir-455-1, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, dre-mir-135b, dre-mir-135a, dre-mir-499, dre-mir-738, dre-mir-429b, dre-mir-1788, dre-mir-196c, dre-mir-107b, dre-mir-455-2, dre-mir-222b, dre-mir-126b, dre-mir-196d, dre-mir-129-3, dre-mir-129-4
Members of the let-7 family and dre-miR-21 showed high levels of expression in the majority of tissues, e. g., dre-miR-21 yielded 21% of muscle miRNAs. [score:3]
Conversely, dre-miR-124, dre-let-7a and dre-mir-21 were ubiquitously expressed. [score:3]
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12
[+] score: 6
We focused our subsequent investigation on two of the miRNAs over-expressed after nerve injury, miR-29b and miR-223, as their increased expression (16 and 55 % increase, respectively) is pertinent when considering the role of miRNAs is to negatively regulate their target genes [18], and unlike miR-21 [19], they have not been extensively studied in the brain. [score:6]
[1 to 20 of 1 sentences]
13
[+] score: 5
By sorting the ‘Expression’ column of the table, the highest expressed miRNA hsa-mir-21 ranks on the top of the table. [score:5]
[1 to 20 of 1 sentences]
14
[+] score: 4
Thum, T. et al. MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts. [score:2]
In the cardiovascular system, miR-29 family has multiple roles: i) is known to be involved in atrial fibrillation [69]; ii) may act as a negative regulator of fibrosis counteracting miR-21 function 43, 70, 71; iii) controls cardiomyocytes apoptosis and aortic aneurism formation 30, 72. [score:2]
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15
[+] score: 3
Other miRNAs from this paper: dre-mir-21-1
Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow -dependent expression of miR-21. [score:3]
[1 to 20 of 1 sentences]
16
[+] score: 3
Other miRNAs from this paper: dre-mir-21-1
Banjo, T. et al. Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow -dependent expression of miR-21. [score:3]
[1 to 20 of 1 sentences]
17
[+] score: 3
Other miRNAs from this paper: dre-mir-21-1, dre-mir-200c
Silibinin suppresses EMT -driven erlotinib resistance by reversing the high miR-21/low miR-200c signature in vivo. [score:3]
[1 to 20 of 1 sentences]
18
[+] score: 2
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-17, hsa-mir-21, hsa-mir-22, hsa-mir-28, hsa-mir-29b-1, hsa-mir-16-2, mmu-let-7g, mmu-let-7i, mmu-mir-1a-1, mmu-mir-29b-1, mmu-mir-124-3, mmu-mir-9-2, mmu-mir-133a-1, mmu-mir-145a, mmu-mir-150, mmu-mir-10b, mmu-mir-195a, mmu-mir-199a-1, hsa-mir-199a-1, mmu-mir-200b, mmu-mir-206, mmu-mir-143, hsa-mir-10a, hsa-mir-10b, hsa-mir-199a-2, hsa-mir-217, hsa-mir-218-1, hsa-mir-223, hsa-mir-200b, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-143, hsa-mir-145, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-150, hsa-mir-195, hsa-mir-206, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-16-1, mmu-mir-16-2, mmu-mir-21a, mmu-mir-22, mmu-mir-29c, rno-let-7d, rno-mir-329, mmu-mir-329, rno-mir-331, mmu-mir-331, rno-mir-148b, mmu-mir-148b, rno-mir-135b, mmu-mir-135b, hsa-mir-200c, hsa-mir-1-1, mmu-mir-1a-2, mmu-mir-10a, mmu-mir-17, mmu-mir-28a, mmu-mir-200c, mmu-mir-218-1, mmu-mir-223, mmu-mir-199a-2, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-7b, mmu-mir-217, hsa-mir-29c, hsa-mir-200a, hsa-mir-365a, mmu-mir-365-1, hsa-mir-365b, hsa-mir-135b, hsa-mir-148b, hsa-mir-331, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, rno-let-7a-1, rno-let-7a-2, rno-let-7b, rno-let-7c-1, rno-let-7c-2, rno-let-7e, rno-let-7f-1, rno-let-7f-2, rno-let-7i, rno-mir-7b, rno-mir-9a-1, rno-mir-9a-3, rno-mir-9a-2, rno-mir-10a, rno-mir-10b, rno-mir-16, rno-mir-17-1, rno-mir-21, rno-mir-22, rno-mir-28, rno-mir-29b-1, rno-mir-29c-1, rno-mir-124-3, rno-mir-124-1, rno-mir-124-2, rno-mir-133a, rno-mir-143, rno-mir-145, rno-mir-150, rno-mir-195, rno-mir-199a, rno-mir-200c, rno-mir-200a, rno-mir-200b, rno-mir-206, rno-mir-217, rno-mir-223, dre-mir-7b, dre-mir-10a, dre-mir-10b-1, dre-mir-217, dre-mir-223, hsa-mir-429, mmu-mir-429, rno-mir-429, mmu-mir-365-2, rno-mir-365, dre-mir-429a, hsa-mir-329-1, hsa-mir-329-2, hsa-mir-451a, mmu-mir-451a, rno-mir-451, dre-mir-451, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-1-2, dre-mir-1-1, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-10b-2, dre-mir-16a, dre-mir-16b, dre-mir-16c, dre-mir-17a-1, dre-mir-17a-2, dre-mir-21-1, dre-mir-22a, dre-mir-22b, dre-mir-29b-1, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-133a-2, dre-mir-133a-1, dre-mir-133b, dre-mir-133c, dre-mir-143, dre-mir-145, dre-mir-150, dre-mir-200a, dre-mir-200b, dre-mir-200c, dre-mir-206-1, dre-mir-206-2, dre-mir-365-1, dre-mir-365-2, dre-mir-365-3, dre-let-7j, dre-mir-135b, rno-mir-1, rno-mir-133b, rno-mir-17-2, mmu-mir-1b, dre-mir-429b, rno-mir-9b-3, rno-mir-9b-1, rno-mir-9b-2, rno-mir-133c, mmu-mir-28c, mmu-mir-28b, hsa-mir-451b, mmu-mir-195b, mmu-mir-133c, mmu-mir-145b, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, mmu-mir-451b, mmu-let-7k, rno-let-7g, rno-mir-29c-2, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3
Olfactory bulb let-7b, let-7c-1, let-7c-2, miR-10a, miR-16, miR-17, miR-21, miR-22, miR-28, miR-29c, miR-124a-1, miR-124a-3, miR-128a, miR-135b, miR-143, miR-148b, miR-150, miR-199a, miR-206, miR-217, miR-223, miR-29b-1, miR-329, miR-331, miR-429, miR-451. [score:1]
Cortex let-7c-1, miR-10a, miR-21, miR-124a-1, miR-128a, miR-135b, miR-150, miR-199a, miR-217, miR-329, miR-451. [score:1]
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[+] score: 2
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-148a, hsa-let-7g, hsa-let-7i, hsa-mir-122, hsa-mir-34c, hsa-mir-148b, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-10c, dre-mir-21-1, dre-mir-122, dre-mir-135c-1, dre-mir-135c-2, dre-mir-148, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-mir-459, hsa-mir-499a, dre-let-7j, dre-mir-499, dre-mir-34c, dre-mir-734, hsa-mir-499b, dre-mir-7146, dre-mir-7147, dre-mir-7148
Slide1: dre-miR-21 is found to be abundant and ubiquitously expressed as compared to some let-7 family members. [score:2]
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20
[+] score: 2
Other miRNAs from this paper: dre-mir-21-1, dre-mir-29a
Resveratrol reduces prostate cancer growth and metastasis by inhibiting the Akt/MicroRNA-21 pathway. [score:2]
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21
[+] score: 1
miRNAs let-7a, miR-100-5p, miR-10b-5p, miR-125b-5p, miR-146a, miR-181a-5p, miR-21, miR-27c-3p and miR-92a-3p were the most abundant miRNAs (>100,000 reads) in the four samples (Excel S1). [score:1]
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