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14 publications mentioning gga-mir-146b

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-146b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 72
DF-1 cells were transfected with miR-146b-3p mimics or inhibitor, and then the expression levels of miR-146b-3p and the GHR gene were detected after 48 h. The results show that overexpression of miR-146b-3p downregulated GHR mRNA expression (Figure 6A), and that the inhibition of endogenous miR-146b-3p increased GHR mRNA expression (Figure 6B). [score:16]
For instance, gga-miR-34c has six targets in the present network, including SOCS3, GCG, APP, THY1, COL11A1 and MYH7B; gga-miR-146b-3p was predicted to target GHR and AKT1; gga-miR-223 was predicted to target FOXO3 and ADAM17; and the predicted target gene of gga-miR-9-5p was TGFBR2. [score:9]
Chicken miR-146b-3p may regulate muscle growth by inhibiting GHR expression. [score:6]
The expression of miR-146b-3p and GHR were detected in our sequencing samples, and the results indicate that their expression reveals a negative relationship (Figure 7). [score:5]
The expression of miR-146b-3p was higher (p < 0.05) in chickens with low body weight than those with high body weight in both WRR and XH chickens (Figure 7B), while the expression of GHR was higher in chickens with high body weight than those with low body weight (Figure 7C). [score:5]
The miR-146b-3p mimics, inhibitors of miR-146b-3p, mimic NC and inhibitor NC were purchased from GenePharma (GenePharma, Suzhou, China). [score:5]
Validation of miR-146b-3p Targeted GHR Gene. [score:3]
In this network, AKT1, another target of miR-146b-3p, was predicted to interact with FOXO3. [score:3]
Figure 6 GHR is a target of miR-146b-3p in chickens. [score:3]
These results indicated that the predicted site of GHR is a target of miR-146b-3p. [score:3]
Comer B. S. Camoretti-Mercado B. Kogut P. C. Halayko A. J. Solway J. Gerthoffer W. T. MicroRNA-146a and microRNA-146b expression and anti-inflammatory function in human airway smooth muscle Am. [score:3]
In human, miR-146b was reported to promote myogenic differentiation and modulate multiple gene targets in muscle cells, and might be involved in the pathogenesis of asthma and Myotonic Dystrophy Type-2 [49, 50, 51]. [score:3]
Figure 7The expression of miR-146b-3p and GHR in breast muscle of two-tail samples of WRR and XH. [score:3]
Interaction networks predicted that GHR was a target of miR-146b-3p, and this was confirmed by dual-luciferase reporter assay and qPCR in our study. [score:2]
The predicted binding site and mutated site of miR-146b-3p in c-GHR is shown. [score:1]
The mutant c-GHR plasmids were generated through changing the miR-146b-3p binding site from ATCCAT to AAGACT (Figure 7C), and mutagenesis was performed by PCR amplification and DpnI digestion to remove the parental DNA. [score:1]
DF-1 cells were co -transfected with 100 ng of the wild-type, mutant 3′-UTR or no-insert control dual-luciferase reporter and 50 nM of the miR-146b-3p mimic using Lipofectamine 3000 reagent in 96-well plates. [score:1]
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[+] score: 36
Other miRNAs from this paper: hsa-let-7d, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-21, hsa-mir-22, hsa-mir-30a, hsa-mir-32, hsa-mir-33a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-147a, hsa-mir-34a, hsa-mir-187, hsa-mir-204, hsa-mir-205, hsa-mir-200b, hsa-mir-23b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-138-2, hsa-mir-142, hsa-mir-144, hsa-mir-125b-2, hsa-mir-138-1, hsa-mir-146a, hsa-mir-190a, hsa-mir-200c, hsa-mir-155, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-365b, hsa-mir-328, gga-mir-33-1, gga-mir-125b-2, gga-mir-155, gga-mir-17, gga-mir-148a, gga-mir-138-1, gga-mir-187, gga-mir-32, gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-190a, gga-mir-204-2, gga-mir-138-2, gga-let-7d, gga-let-7f, gga-mir-146a, gga-mir-205b, gga-mir-200a, gga-mir-200b, gga-mir-34a, gga-mir-30e, gga-mir-30c-1, gga-mir-205a, gga-mir-204-1, gga-mir-23b, gga-mir-142, hsa-mir-449a, hsa-mir-489, hsa-mir-146b, hsa-mir-548a-1, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-33b, hsa-mir-449b, gga-mir-147, gga-mir-489, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-144, gga-mir-460a, hsa-mir-147b, hsa-mir-190b, gga-mir-22, gga-mir-460b, gga-mir-1662, gga-mir-1684a, gga-mir-449c, gga-mir-146c, gga-mir-449b, gga-mir-2954, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az, gga-mir-365b, gga-mir-33-2, gga-mir-125b-1, gga-mir-190b, gga-mir-449d, gga-mir-205c
Significant upregulation of miR-146b-5p and its corresponding downregulation of CDK2AP1 were observed in the AS pulmonary arteries in our findings, which is consistent with Zhong et al., who reported that significant upregulation of miR-205 targeted the downregulation of CDK2AP1 in the laryngeal squamous cell carcinoma [43]. [score:15]
The miR-146b and miR-146a these two members of miR-146 family, which negatively regulate expressions of inflammatory genes in some cell types containing airway smooth muscle, endothelial, epithelial cells and fibroblasts, are upregulated in a murine asthma mo del [34]. [score:7]
Likewise, from the miRNA-mRNA association, the under expressed genes LZTFL1, JAZF1, THBS2 and RPS14 were associated with microRNAs (miR-146b-5p, miR-1684a-3p, miR-460b-3p, miR-30e-5p, miR-33-5p, miR-148a-5p, miR-32-5p, miR-155 and miR-144-3p) that were down-regulated in pulmonary arteries (Figure 4). [score:6]
Considering the differential expressions of miR-155, miR-23b-3p, miR-146b-5p and miR-146b-3p found in the AS broiler pulmonary artery tissues in this study, we proposed these four miRNAs may also serve as a key regulator to pulmonary artery remo deling during AS progress. [score:4]
In this study, we found that miR-155, miR-23b-3p, miR-146b-5p and miR-146b-3p upregulated in AS broilers’ pulmonary arteries tissue. [score:4]
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[+] score: 25
Putative target genes of gga-miR-181a, gga-miR-26a, gga-miR-221, gga-miR-222, gga-miR-155, gga-miR-146b, and gga-miR-146c were predicted by TargetScan (Version 6.0) [31]. [score:5]
In humans, elevated expression of miR-146b was observed in malignant myoepithelioma of breast [71] as well as papillary thyroid carcinoma [63], [72]. [score:3]
In our study, miR-146b only showed an increasing trend, whereas its isoform, miR-146c, which has been only reported in chicken and zebra finch to date, showed significantly elevated expression level in tumorous spleen and MD lymphoma. [score:3]
H. Expression of gga-miR-146b. [score:3]
The differential expression of miR-146b and miR-146c between MDV-infected and non-infected samples was analyzed. [score:3]
In the current study, miR-146b showed an increasing trend, and miR-146c was significantly up-regulated in tumorous spleens and MD lymphomas compared to non-infected spleens. [score:3]
gga-miR-146b showed a trend toward being higher, and gga-miR-146c was up-regulated in tumorous spleen and MD lymphoma compared to non-infected spleens (Figure 2F, G). [score:3]
Nikiforova et al. reported that miR-146b was overexpressed at least 2-fold in papillary thyroid cancer compared with benign hyperplastic nodules [73]. [score:2]
[1 to 20 of 8 sentences]
[+] score: 21
Seventeen miRNAs with different expression levels were selected randomly; one was not significantly expressed (miR-1786), the others are sixteen SDE miRNAs including six up-regulated (miR-375, -3523, -125b-5p, -130b-5p, -456-3p, and -460a-5p), and ten down-regulated miRNAs (miR-146b-5p, -24-3p, -451, -126-5p, -2188-5p, -33-3p, -22-3p, -148a-3p, -21-5p, and -10a-5p) (Fig. 4). [score:11]
Consistent with the fold-change in miR-146b-5p, miR-24-3b was down-regulated (−7.39-fold) in 30-week-old chickens. [score:4]
The SDE miRNAs with high fold-changes were all down-regulated in L30, including miR-146b-5p (−8.50-fold), miR-24-3p (−7.39-fold), miR-146a-5p (−5.96-fold), miR-221-5p (−5.85-fold), miR-7b (−5.35-fold), miR-147 (−5.11-fold), miR-20-5p (−4.59-fold), and miR-140b-5p (−4.57-fold). [score:4]
Seven conserved families all were DE with P ≤ 0.05, including let-7 (let-7a, -7b, -7c,-7f, -7g, -7i, -7j, and -7k), miR-130 (miR-130a, and -130b), miR-146 (miR-146a, -146b, and -146c), miR-15 (miR-15a, -15b, and -15c), miR-181 (miR-181a and -181b), miR-29 (miR-29a, -29b and -29c), and miR-30 (miR-30a, -30b, -30c, -30d, and -30e). [score:1]
For instance, miR-146b-5p (also known as miR-146b) is located in an intergenic region (22,683,802–22,683,906) on chicken chromosome 6 and is found in most vertebrate species (including mammals). [score:1]
[1 to 20 of 5 sentences]
[+] score: 17
The DEM miR-27 has an inhibitory role with regard to adipogenesis in mouse and human multipotent adipose-derived stem (hMADS) cells 30, and overexpression of miR-27 specifically inhibited adipocyte formation in mice 31. miR-146b has been related to the differentiation of 3T3-L1 cells 29. [score:7]
In this study, some of the DEMs relevant to the 11 intersection genes may be strong candidates for regulating AbF because miR-133c-3p, miR-133a-3p, miR-200a-3p, and miR-146b-5p were shown by independent qPCRs to be differentially expressed in birds with very high and very low AbF contents. [score:4]
Eleven of these (miR-204, miR-19a-3p, miR-19b-3p, miR-30d, miR-26a, miR-122-5p, miR-103-3p, miR-27b-3p, miR-92-3p, miR-142-3p, and miR-17-5p) have been implicated, directly or indirectly, in fat deposition; 9 showed a high fold-change (miR-3535, miR-144-3p, miR-30e-5p, miR-301b-3p, miR-215-5p, miR-200a-3p, miR-133a-3p, miR-133c-3p, and miR-146b-5p). [score:3]
However, many important candidate miRNAs related to lipid mechanism (e. g., gga-miR-301b-3p, gga-miR-130b-3p, gga-miR-30a-5p, gga-miR-142, gga-miR-146b, gga-miR-103, gga -miR-26a, etc. ) [score:1]
Potentially novel miRNAs (gga-miR-3535, miR-30e-5p, miR-301b-3p, miR-215-5p, miR-200a-3p, miR-133a-3p, miR-133c-3p, and miR-146b-5p) and genes (LAMA2, RAP1B, PECR, AKT1, ITGALL and CHAD) related to abdominal adipose tissue were also identified. [score:1]
Changes in four lipid-related DEMs identified by deep sequencing (gga-miR-122-5p, miR-103-3p, miR-27b-3p, and miR-146b-5p) were confirmed by qPCR. [score:1]
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[+] score: 14
miR-146 is an immune system regulator and plays a key role in regulating different types of diseases (Jopling et al., 2005). [score:5]
NF-κB -dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. [score:5]
Divergent intracellular pathways regulate interleukin-1beta -induced miR-146a and miR-146b expression and chemokine release in human alveolar epithelial cells. [score:4]
[1 to 20 of 3 sentences]
[+] score: 9
Other miRNAs from this paper: gga-mir-146a, gga-mir-21
Expression analysis of four miRNA genes including mir-21, mir-146a, mir-146b, and mir-146c1, as well as IL-10 was assessed in TECs exposed to either LAMPs or live organisms. [score:3]
Expression of miRNA21, miRNA146b and miRNA146c1 did not differ from media controls at any time point in any exposure group (data not shown). [score:3]
No difference in expression was observed for miRNA146b, miRNA146c1 or miRNA21. [score:3]
[1 to 20 of 3 sentences]
[+] score: 8
For instance, few proteins (IRAK1, IRAK2, and TRAF6) within TLR signaling have been confirmed as direct targets of miR-146 (O'Neill et al., 2011); signal molecules MyD88, TAB2, SHIP1, and SOCS1 were targets of miR-155 (Eulalio et al., 2012); and cytokines IL-6 and IL-10 are targeted by Let-7 (Stae del and Darfeuille, 2013). [score:8]
[1 to 20 of 1 sentences]
[+] score: 6
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-106a, hsa-mir-16-2, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-23b, hsa-mir-27b, hsa-mir-122, hsa-mir-125b-1, hsa-mir-140, hsa-mir-125b-2, hsa-mir-136, hsa-mir-146a, hsa-mir-150, hsa-mir-206, hsa-mir-155, hsa-mir-181b-2, hsa-mir-106b, hsa-mir-302a, hsa-mir-34b, hsa-mir-34c, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-367, gga-let-7i, gga-let-7a-3, gga-let-7b, gga-let-7c, gga-mir-125b-2, gga-mir-155, gga-mir-222a, gga-mir-221, gga-mir-92-1, gga-mir-19b, gga-mir-20a, gga-mir-19a, gga-mir-18a, gga-mir-17, gga-mir-16-1, gga-mir-15a, gga-mir-1a-2, gga-mir-206, gga-mir-223, gga-mir-106, gga-mir-302a, gga-mir-181a-1, gga-mir-181b-1, gga-mir-16-2, gga-mir-15b, gga-mir-140, gga-let-7g, gga-let-7d, gga-let-7f, gga-let-7a-1, gga-mir-146a, gga-mir-181b-2, gga-mir-181a-2, gga-mir-1a-1, gga-mir-1b, gga-let-7a-2, gga-mir-34b, gga-mir-34c, gga-let-7j, gga-let-7k, gga-mir-23b, gga-mir-27b, gga-mir-24, gga-mir-122-1, gga-mir-122-2, hsa-mir-429, hsa-mir-449a, hsa-mir-146b, hsa-mir-507, hsa-mir-455, hsa-mir-92b, hsa-mir-449b, gga-mir-302b, gga-mir-302c, gga-mir-302d, gga-mir-455, gga-mir-367, gga-mir-429, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-1458, gga-mir-1576, gga-mir-1612, gga-mir-1636, gga-mir-449c, gga-mir-1711, gga-mir-1729, gga-mir-1798, gga-mir-122b, gga-mir-1811, gga-mir-146c, gga-mir-15c, gga-mir-449b, gga-mir-222b, gga-mir-92-2, gga-mir-125b-1, gga-mir-449d, gga-let-7l-1, gga-let-7l-2, gga-mir-122b-1, gga-mir-122b-2
Interestingly, some of these differentially expressed miRNAs, including miR-146, have been previously reported to be associated with immune-related signal pathways in mammals. [score:3]
After exposure of THP-1 (human acute monocytic leukemia cell line) cells to lipopolysaccharides (LPS), miR-146 was identified as an inhibitor of signalling proteins of the innate immune responses by NF-kappaB [14]. [score:3]
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[+] score: 3
Nata et al. (9) reported that overexpression of miR-146b activated the NF-κB pathway, improved epithelial barrier function, ameliorated intestinal inflammation in dextran sulfate sodium -induced colitis mice. [score:3]
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[+] score: 2
[40] It was revealed that miR-146, [41] miR-155, [42] and miR-203 [43] regulate arthritic inflammatory response. [score:2]
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[+] score: 2
Among these genes, represented in clade1 and clade2, are a number of miRNAs exhibiting strong expression in B2 cells (mir-147, mir-146b, mir-1618, mir-200a, mir-1649, and mir-1648a) compared to the B19 samples. [score:2]
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[+] score: 1
Similarly, other miRNA families exhibited vast read ranges, such as the gga-miR146 (91–235,844 reads) and gga-miR-30 (886–164,361 reads) families (Additional file 1: Table S2). [score:1]
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[+] score: 1
Moreover, the activation of TLR signals can also induce microRNAs (such as miR-146, miR-155 and miR-223) that can feedback the components in the TLR signalling system[9, 10]. [score:1]
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