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22 publications mentioning mmu-mir-1224

Open access articles that are associated with the species Mus musculus and mention the gene name mir-1224. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 85
b miRNA RT-qPCR analysis showing the complete regulation of Hsa-miR-93, Hsa-miR-20a, Hsa-miR-125b and Hsa-miR-27b and, significant increase in the expression of Hsa-miR-1260 and Hsa-miR-1224-3p in metastatic tumors as compared to the non-metastatic xenograftTo validate the altered expression levels observed with whole genome miRNA array, we examined the expression levels of select miRNAs including hsa-miR-1260, hsa-miR-1224-3p (showing significant upregulation; see Fig.   2), hsa-miR-93, hsa-miR-20a, hsa-miR-125b, hsa-miR-27b (showing significant downregulation; see Fig.   3a), using individual miRNA QPCR analysis. [score:13]
b miRNA RT-qPCR analysis showing the complete regulation of Hsa-miR-93, Hsa-miR-20a, Hsa-miR-125b and Hsa-miR-27b and, significant increase in the expression of Hsa-miR-1260 and Hsa-miR-1224-3p in metastatic tumors as compared to the non-metastatic xenograft To validate the altered expression levels observed with whole genome miRNA array, we examined the expression levels of select miRNAs including hsa-miR-1260, hsa-miR-1224-3p (showing significant upregulation; see Fig.   2), hsa-miR-93, hsa-miR-20a, hsa-miR-125b, hsa-miR-27b (showing significant downregulation; see Fig.   3a), using individual miRNA QPCR analysis. [score:13]
In order to validate the miRNA expression obtained from whole genome profiling, expression of selected metastamiRs, including hsa-miR-1224-3p, hsa-miR-1260 (both significantly upregulated), hsa-miR-125b, hsa-miR-27b, hsa-miR-93,and hsa-miR-20a (all significantly downregulated) were confirmed using QPCR. [score:11]
To define the effect of characterized metastamiRs on the putative target proteins, we adopted two approaches: (i) inhibited hsa-miR-1224-3p or hsa-miR-1260 (both significantly upregulated) and (ii) functionally mimicked hsa-miR-125b, hsa-miR-27b, hsa-miR-93 or hsa-miR-20a (all significantly downregulated) and examined for the miRNA -dependent modulations in protein targets. [score:11]
Thus, we validated our microarray results with RT-qPCR for upregulation (Hsa-miR-1260; Hsa-miR-1224-3p) and downregulation (Hsa-miR-20a, Hsa-miR-27b, Hsa-miR-125b, Hsa-miR-93) profiles (see Fig.   3b). [score:7]
miRNA mimic (hsa-miR-125b, hsa-miR-27b, hsa-miR-93, hsa-miR-20a) and inhibitor (hsa-miR-1224-3p, hsa-miR-1260) approach for select miRNAs revealed the direct influence of the altered metastamiRs in the regulation of identified protein targets. [score:7]
c Histograms of mean cell–Alexa Fluor intensity showing alterations in the expression of (i) ADAMTS-1 and CREB with hsa-miR-1224-3p inhibition and, (ii) ADAMTS-1, ASK1, FOSB and AKT-1 with hsa-miR-1260 inhibition. [score:7]
Transient transfection of MSDACs with hsa-miR-125b-, hsa-miR-27b-, hsa-miR-93- or hsa-miR-20a- mimics (MISSION® microRNA Mimics, Sigma-Aldrich) as well as hsa-miR-1224-3p- and hsa-miR-1260 -inhibitors (MISSION® Synthetic miRNA Inhibitors, Sigma-Aldrich) were carried out by using either TurboFectin 8.0 reagent (Origene) or Neon electroporation transfection system (Life Technologies). [score:5]
Next, MSDACs transiently transfected with inhibitors for hsa-miR-1224-3p or hsa-miR-1260 (both showed profound induction in metastatic tumors) and examined for the alterations in protein targets. [score:5]
Conversely, we observed a significant (P < 0.001) upregulation of hsa-miR-1260 and hsa-miR-1224-3p in metastatic tumor compared with the non-metastatic control (Fig.   3b). [score:3]
Inhibiting hsa-miR-1224-3p resulted in the significant (P < 0.001) induction of ADAMTS-1 and CREB (Fig.   6c i). [score:3]
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2
[+] score: 18
The primers are listed in Supplementary Table 1. In vivo monitoring of pri-miRNA processingThe miR-219-Glo, miR-1224-Glo or control-Glo (pmirGLO, Promega) vector was transfected together with TLX -expressing vector or TLX siRNA -expressing vector. [score:4]
The primers are listed in Supplementary Table 1. The miR-219-Glo, miR-1224-Glo or control-Glo (pmirGLO, Promega) vector was transfected together with TLX -expressing vector or TLX siRNA -expressing vector. [score:4]
Expression of TLX had no effect on luciferase activity of miR-1224-Glo, a reporter that contains part of miR-1224, a miRtron that is processed into pre-miRNA independent of Drosha cleavage 33 (Fig. 1f). [score:3]
In contrast to overexpression of TLX, knockdown of TLX in NSCs promoted miR-219 processing, as shown by reduced luciferase activity of miR-219-Glo, compared to control RNA -treated cells (Fig. 1g), but had no effect on luciferase activity of miR-1224-Glo (Fig. 1g). [score:3]
The relative luciferase activity in NSCs transfected with a vector expressing siC or siTLX, together with the control-Glo, miR-219-Glo reporter or miR-1224-Glo reporter control; n=4. [score:3]
miR-1224-Glo was included as a negative control. [score:1]
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3
[+] score: 17
Among the list, members of the miR-29 family, miR-203, miR-762, and miR-1224, showed upregulation, whereas members of the miR-107 family, miR-127 and miR-130a/b, miR-342-3p, miR-351, miR-379, miR-455, and miR-467a, were downregulated in both strains. [score:7]
Transfection of miR-1224 results in a decrease in basal tumour necrosis factor-α (TNF-α) promoter reporter gene activity and a downregulation of LPS -induced TNF-α mRNA in RAW264.7 cells [44]. [score:4]
miR-1224 is highly expressed in mouse spleen, kidney, and lung [44]. [score:3]
It is interesting to observe the enhanced expression of miR-762 and miR-1224 in the LW. [score:3]
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4
[+] score: 15
Seven conserved DE miRs are upregulated during pneumonia and are expressed at moderate to high levels in lung neutrophils (average normalized mean expression above 5): mmu-miR-1224-5p, mmu-miR-188-5p, mmu-miR-139-5p, mmu-miR-15b-5p, mmu-miR-721, mmu-miR-18a-5p, and mmu-miR-130b-3p. [score:8]
We identified a network containing seven upregulated conserved miRs (mmu-miR-1224-5p, mmu-miR-188-5p, mmu-miR-139-5p, mmu-miR-15b-5p, mmu-miR-721, mmu-miR-18a-5p and mmu-miR-130b-3p) and another network consisting of downregulated miRs belonging to 3 highly conserved miR families (let-7, mir-30 and mir-34). [score:7]
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5
[+] score: 13
Among the differentially expressed miRNAs in lungs, most were correlated with immune system regulation, including miR-223 (regulation of the immune response), miR-1224 (regulation of tumor necrosis factor), miR-150 (differentiating stem cells towards megakaryocytes and control of B and T cell differentiation), miR-200a (regulation of immune response). [score:7]
miR-1224, with the function as: ‘negative regulator of tumour necrosis factor’, which has been shown to regulate tumour necrosis factor-α (TNF-α) gene expression by modulating Sp1, might be involved in regulating the LPS -mediated inflammatory responses [48]. [score:6]
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6
[+] score: 13
Figure 4 A. Histograms of individual miRNA QPCR analysis showing circulating levels of randomly selected miRNAs (miR-20a, miR-27b, miR-1224-3p, miR-1260, and miR-93) in the serum of animals with non-metastatic primary disease or with high-risk metastatic disease. [score:5]
Compared with the non-metastatic favorable disease animals, we observed marginal variations in the expression of miR-20a, miR-27b, miR-93, miR-1260, and miR-1224 (Figure 4A). [score:4]
For the present study, we used QPCR to confirm expression of selected miRNAs, including hsa-miR-1224-3p, hsa-miR-1260, hsa-miR-27b, hsa-miR-93, and hsa-miR-20a. [score:3]
B. Correlation analysis of the serum-circulating profiles of miR-20a, miR-27b, miR-1224-3p, miR-1260, and miR-93 observed using the miRnome approach. [score:1]
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7
[+] score: 9
The second gene of interest is Nmnat1, which was downregulated (–1.24-fold change and P=0.01), whereas its three predicted target miRNAs (mir-1224, mir-431 and mir-743a) were upregulated (Fig. 7). [score:9]
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8
[+] score: 9
While Hunsberger et al. also report that miR-1224 is upregulated in response to cerebral ischemia in rats [33], it has yet to be determined whether these microRNAs are causative or merely responding to injury. [score:4]
Panel E shows miR-1224. [score:1]
We have demonstrated highly reproducible, progressive, and concordant elevation of miR-714, miR-1188, miR-1897-3p, miR-877*, and miR-1224 in plasma and kidneys at 3, 6 and 24 hour after acute kidney injury. [score:1]
miR-1897-3p, miR-1188, miR-714, miR-877*, and miR-1224 increased time dependently in both kidneys and plasma. [score:1]
Of the eleven microRNAs retested, five (miR-1188, miR-1897-3p, miR-714, miR-877*, and miR-1224) confirmed in both kidney and plasma (Figure 5). [score:1]
The mo del produced highly reproducible, progressive, concordant elevation of miR-714, miR–1188, miR-1897-3p, miR-877*, and miR-1224 in plasma and kidneys at 3, 6 and 24 hours after acute kidney injury. [score:1]
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9
[+] score: 8
Other miRNAs from this paper: mmu-mir-30a, mmu-mir-101a, mmu-mir-125a, mmu-mir-125b-2, mmu-mir-132, mmu-mir-134, mmu-mir-135a-1, mmu-mir-138-2, mmu-mir-142a, mmu-mir-150, mmu-mir-154, mmu-mir-182, mmu-mir-183, mmu-mir-24-1, mmu-mir-194-1, mmu-mir-200b, mmu-mir-122, mmu-mir-296, mmu-mir-21a, mmu-mir-27a, mmu-mir-92a-2, mmu-mir-96, rno-mir-322-1, mmu-mir-322, rno-mir-330, mmu-mir-330, rno-mir-339, mmu-mir-339, rno-mir-342, mmu-mir-342, rno-mir-135b, mmu-mir-135b, mmu-mir-19a, mmu-mir-100, mmu-mir-139, mmu-mir-212, mmu-mir-181a-1, mmu-mir-214, mmu-mir-224, mmu-mir-135a-2, mmu-mir-92a-1, mmu-mir-138-1, mmu-mir-181b-1, mmu-mir-125b-1, mmu-mir-194-2, mmu-mir-377, mmu-mir-383, mmu-mir-181b-2, rno-mir-19a, rno-mir-21, rno-mir-24-1, rno-mir-27a, rno-mir-30a, rno-mir-92a-1, rno-mir-92a-2, rno-mir-96, rno-mir-100, rno-mir-101a, rno-mir-122, rno-mir-125a, rno-mir-125b-1, rno-mir-125b-2, rno-mir-132, rno-mir-134, rno-mir-135a, rno-mir-138-2, rno-mir-138-1, rno-mir-139, rno-mir-142, rno-mir-150, rno-mir-154, rno-mir-181b-1, rno-mir-181b-2, rno-mir-183, rno-mir-194-1, rno-mir-194-2, rno-mir-200b, rno-mir-212, rno-mir-181a-1, rno-mir-214, rno-mir-296, mmu-mir-376b, mmu-mir-370, mmu-mir-433, rno-mir-433, mmu-mir-466a, rno-mir-383, rno-mir-224, mmu-mir-483, rno-mir-483, rno-mir-370, rno-mir-377, mmu-mir-542, rno-mir-542-1, mmu-mir-494, mmu-mir-20b, mmu-mir-503, rno-mir-494, rno-mir-376b, rno-mir-20b, rno-mir-503-1, mmu-mir-551b, mmu-mir-672, mmu-mir-455, mmu-mir-490, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-504, mmu-mir-466d, mmu-mir-872, mmu-mir-877, rno-mir-466b-1, rno-mir-466b-2, rno-mir-466c, rno-mir-872, rno-mir-877, rno-mir-182, rno-mir-455, rno-mir-672, mmu-mir-466l, mmu-mir-466i, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-466j, rno-mir-551b, rno-mir-490, rno-mir-1224, rno-mir-504, mmu-mir-466m, mmu-mir-466o, mmu-mir-466c-2, mmu-mir-466b-4, mmu-mir-466b-5, mmu-mir-466b-6, mmu-mir-466b-7, mmu-mir-466p, mmu-mir-466n, mmu-mir-466b-8, rno-mir-466d, mmu-mir-466q, mmu-mir-21b, mmu-mir-21c, mmu-mir-142b, mmu-mir-466c-3, rno-mir-322-2, rno-mir-503-2, rno-mir-466b-3, rno-mir-466b-4, rno-mir-542-2, rno-mir-542-3
Both ACTH and 17α-E2 up-regulated the expression of miRNA-212, miRNA-132, miRNA-154, miRNA-494, miRNA-872, miRNA-194, and miRNA-24-1, but reduced the expression of miRNA-322, miRNA-20b, miRNA-339, miRNA-27a, miRNA-551b, and miRNA-1224. [score:8]
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10
[+] score: 7
The ten most up-regulated miRNAs included mmu-miR-205-5p, mmu-miR-222-3p, mmu-miR-205-3p, mmu-miR-146b-5p, mmu-miR-21-5p, mmu-miR-21-3p, mmu-miR-221-3p, mmu-miR-140-3p, mmu-miR-142-5p, and mmu-miR-140-5p and the ten most down-regulated miRNAs comprised mmu-miR-211-5p, mmu-miR-3096-5p, mmu-miR-711, mmu-miR-466h-5p, mmu-miR-130b-3p, mmu-miR-3082-5p, mmu-miR-1199-5p, mmu-miR-669b-5p, mmu-miR-1187, and mmu-miR-1224-5p (Table 1). [score:7]
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11
[+] score: 5
Out of 12 miRNA families that were predicted to target the PRKAG1 sense promoter in both human and mouse, nine (miR-718, miR-1224, miR-188, miR-346, miR-296, miR-671, miR-221, miR-1306, miR-506) can form highly stable duplex structures with their target sites (MFE ≤ −30 kcal/mol) in both organisms. [score:5]
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12
[+] score: 5
For example, miR-7 regulates the expression of A53T α-synuclein [51], miR-7 and -153 participate in SNCA transcription [23, 51], the function of miR-1224, -184 and let-7i-3p/5p is regulated by leucine-rich repeat kinase 2 (LRRK2) [52, 53], and miR-127-5p and -16-5p play their roles in glucocerebrosidase (GBA) pathway [54]. [score:5]
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13
[+] score: 5
To test whether the PMIS inhibits noncanonical miRs, inhibitors were made to two miRs that do not require Drosha (miR-877-5p and miR-1224-5p) and one that does not require Dicer (miR-451a). [score:5]
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14
[+] score: 5
Furthermore, we identified miR-9, miR-455, and miR-1224 as microRNAs downregulated upon myelination and reduced following Dicer ablation from Schwann cells. [score:4]
Only three microRNAs met these criteria: miR-9, miR-455, and miR-1224. [score:1]
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15
[+] score: 4
Down-regulated 3 miRNAs were mmu-miR-1224, mmu-miR-9-3p, and mmu-miR-26a in the order of the significance score by SAM. [score:4]
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16
[+] score: 4
MiR-686 and miR-1224 both showed increased expression in males and females, as predicted by the miRNA arrays. [score:3]
We also examined miR-682 that increased in males, miR-686 and miR-1224 that increased in males and females, and miR-883a-3p that increased in females (1B). [score:1]
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17
[+] score: 4
MiR-27b, miR-214, miR-199a-3p, miR-182, miR-183, miR-200a, and miR-322 were found to be downregulated, whereas miR-705 and miR-1224 were increased after 4 weeks of alcohol feeding in mice [26]. [score:4]
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18
[+] score: 3
They are distinguished by additional suffixes (e. g. hsa-miR-1224-5p (5'arm) and hsa-miR-1224-3p (3'arm)). [score:1]
If complete names are used, e. g. hsa-miR-1224-5p, the author likely means the 5'arm predominant mature form of human miRNA-1224. [score:1]
On the other hand, an incomplete form e. g. miR-1224 could mean precursor or mature microRNAs, the 3' or the 5' variant or an unspecified variant of microRNA 1224 in some species depending on the context. [score:1]
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19
[+] score: 1
Additionally, selected miRs that were significantly altered in our microarray, such as miR-26b, miR-27a, miR-143, miR-150, miR-328, miR-341*, miR-680 and miR-1224, were validated. [score:1]
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20
[+] score: 1
MiR-709, miR-1224, and miR-342-3p/5p are increased several-fold in the DP subset, while miR-150 and miR-342-3p are increased in all the thymocyte subsets. [score:1]
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21
[+] score: 1
Note that there is not evidence for small RNA generation across most of the aligned species, and only in a small number of the species exhibit a classic "saddle-shaped" evolutionary profile in which the hairpin loop clearly evolves more quickly than does the hairpin arms (e. g. as is seen for mir-1224, mir-3064, and mir-877). [score:1]
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22
[+] score: 1
Some miRNAs (mitrons) such as miR-877, miR-1224, and miR-1226 are independent of the canonical miRNA biogenesis pathway but dependent on the splicing process [25]. [score:1]
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