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22 publications mentioning bta-mir-20a

Open access articles that are associated with the species Bos taurus and mention the gene name mir-20a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 162
Down-regulated miR-20a-5p was found to potentially target eight up-regulated DEG (ELK4 (ETS transcription factor), ELOVL6 (fatty acid elongase 6), ETV1 (ETS variant 1), KDM6B (lysine demethylase 6B), KIAA1524, LONP2 (lon peptidase 2, peroxisomal), M6PR (mannose-6-phosphate receptor, cation dependent), USP12 (ubiquitin specific peptidase 12)) in LF-SO cows and miR-142-5p was found to potentially target four up-regulated DEG (ELK4, ELOVL6, ETV1, PIK3CD (phosphatidylinositol 3-kinase catalytic delta polypeptide)). [score:14]
miRNA Gene symbol Gene name Functional category miR-20a-5p, ELK4 ETS-domain protein Replication/Transcription/Translation miR-142-5p (SRF accessory protein 1) miR-20a-5p, ELOVL6 ELOVL fatty acid elongase 6 Cellular lipid metabolism & transport miR-142-5p miR-20a-5p, ETV1 ETS variant 1 Replication/Transcription/Translation miR-142-5p miR-20a-5p KDM6B Lysine (K)-specific demethylase 6B Replication/Transcription/Translation miR-20a-5p KIAA1524 KIAA1524 ortholog Cell cycle,cell growth, proliferation, differentiation & death miR-20a-5p LONP2 Lon peptidase 2n peroxisomal Cellular protein metabolism & transport miR-20a-5p M6PR Mannose-6-phophatase receptor (cation dependent) Cellular carbohydrate metabolism & transport miR-20a-5p USP12 Ubiquitin specific peptidase 12 Cellular protein metabolism & transport miR-142-5p PIK3CD Phosphoinositide-3-kinase, catalytic, delta polypeptide Immune, inflammatory and stress response Impact of miR-20a-5p and miR-142-5p on the expression of ELOVL6, gene involved in lipid metabolismTo investigate whether miR-20a-5p and/or miR-142-5p could regulate the expression of ELOVL6 in mammary epithelial cells, miRNAs were over-expressed individually or together using miRNA mimics in culture bovine mammary epithelial monolayers (BME-UV1). [score:12]
However, the in vivo down-regulation of miR-20a-5p and miR-142-5p, and the up-regulation of ELOVL6, were observed in a context of lactation, which was not reproduced in cultured cells where the cellular context may influence miRNA function [73]. [score:7]
miRNA Gene symbol Gene name Functional category miR-20a-5p, ELK4 ETS-domain protein Replication/Transcription/Translation miR-142-5p (SRF accessory protein 1) miR-20a-5p, ELOVL6 ELOVL fatty acid elongase 6 Cellular lipid metabolism & transport miR-142-5p miR-20a-5p, ETV1 ETS variant 1 Replication/Transcription/Translation miR-142-5p miR-20a-5p KDM6B Lysine (K)-specific demethylase 6B Replication/Transcription/Translation miR-20a-5p KIAA1524 KIAA1524 ortholog Cell cycle,cell growth, proliferation, differentiation & death miR-20a-5p LONP2 Lon peptidase 2n peroxisomal Cellular protein metabolism & transport miR-20a-5p M6PR Mannose-6-phophatase receptor (cation dependent) Cellular carbohydrate metabolism & transport miR-20a-5p USP12 Ubiquitin specific peptidase 12 Cellular protein metabolism & transport miR-142-5p PIK3CD Phosphoinositide-3-kinase, catalytic, delta polypeptide Immune, inflammatory and stress response ABCA1: ATP-Binding Cassette subfamily A (ABC1) member1, BTN1A1: BuTyrophiliN subfamily 1, member A1, LPIN1: LiPIN1, PPARγ: Peroxisome Proliferator-Activated Receptor gamma, PPARGC1B: Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Beta, SCD: Steroyl-CoA Desaturase, VLDLR: Very Low Density Lipoprotein Lipase Receptor. [score:7]
MiR-20a-5p may act i) on lipoprotein metabolism (by targeting APOBEC4 (apolipoprotein B mRNA editing enzyme catalytic polypeptide like 4), LDLR (low density lipoprotein receptor) and VLDLR (very low density lipoprotein receptor)), ii) on fatty acid desaturation (by targeting SCD5 (stearoyl-CoA desaturase 5)), and iii) on lipid secretion (by targeting BTN1A1 (butyrophilin subfamily 1 member A1), a major constituent of the milk fat globule membrane). [score:6]
Both miR-20a-5p and miR-142-5p are predicted to target ELK4 and ETV1 which are known to be involved in replication, transcription and translation. [score:5]
Among them, twenty-three predicted targets for miR-20a-5p and miR-142-5p have been identified, using DIANA software, which three were targeted by the two miRNAs. [score:5]
**0.01miR-20a-5p and miR-142-5p are predicted to target genes differentially expressed by sunflower oilIn order to investigate the functional role of miR-20a-5p and miR-142-5p, computational applications were used to predict their targets. [score:5]
It can nevertheless be hypothesized that a weaker expression of miR-20a-5p and miR-142-5p may have contributed to the increased expression of ELOVL6 in the mammary glands of cows receiving the LF-SO diet. [score:5]
0185511.g005 Fig 5Effect of miR-20a-5p and miR-142-5p over -expression on ELOVL6 expression in BME-UV1 cells. [score:5]
Members of the PPAR family such as PPARα, PPARγ and the co-activator PPARGC1B (peroxisome proliferative activated receptor, gamma, coactivator 1 beta), are known to be transcription factors involved in the control of genes coding for lipogenic enzymes, are targeted by miR-142-5p, and are predicted to be targeted by miR-20a-5p [62]. [score:5]
miR-20a-5p and miR-142-5p are predicted to target genes differentially expressed by sunflower oil. [score:5]
Effect of miR-20a-5p and miR-142-5p over -expression on ELOVL6 expression in BME-UV1 cells. [score:5]
Interestingly, both miR-20a-5p and miR-142-5p potentially target ELOVL6, a member of the family of fatty acid elongases [67], which are involved in lipid metabolism and were over-expressed in our LF-SO samples. [score:5]
Among the eight studied miRNAs, RT-qPCR validations confirmed that miR-20a-5p (p = 0.08) and miR-142-5p (p = 0.03) were significantly down-regulated by sunflower oil supplementation. [score:4]
To investigate whether miR-20a-5p and/or miR-142-5p could regulate the expression of ELOVL6 in mammary epithelial cells, miRNAs were over-expressed individually or together using miRNA mimics in culture bovine mammary epithelial monolayers (BME-UV1). [score:4]
Here, in response to oil supplementation, miR-20a-5p will be able to regulate DNA methylation by targeting a demethylase. [score:4]
MiR-20a-5p, which was differentially expressed in our study, belongs to the miR-17/92 cluster that has mainly been described for its function as an oncomiR, but is also important to cell cycle proliferation, apoptosis and other biological processes in several organs [58]. [score:3]
LPIN1 (lipin 1) is also potentially targeted by miR-20a-5p. [score:3]
Predicted targets of miR-20a-5p and miR-142-5p involved in the lipid metabolism. [score:3]
ELOVL6 expression after transfection of miR-20a-5p and miR-142-5p (B) or miR-1-3p (C). [score:3]
Interestingly, among the predicted targets of miR-20a-5p and miR-142-5p are nine DEG previously identified in the same samples using previous transcriptomic analysis [46] (Table 3). [score:3]
miRNA Gene symbol Gene name miR-20a-5p ABCA1 ATP-Binding cassette, sub -family A (ABC1), member 1 APOBEC4 Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 4 (putative) APP Amyloid beta (A4) precursor protein BTN1A1 Butyrophilin, subfamily 1, member 1 ELOVL6 ELOVL fatty acid elongase 6 LDLR Low density lipoprotein receptor LPIN1 Lipin 1 PPARA Peroxisome proliferator-activated receptor alpha PPARG Peroxisome proliferator-activated receptor gamma PPARGC1B Peroxisome proliferator-activated receptor gamma, coactivator 1 beta SCD5 Steroyl-CoA desaturase VLDLR Very low density lipoprotein receptor miR-142-5p ABCA1 ATP-Binding cassette, sub -family A (ABC1), member 1 ACADL Acyl-CoA dehydrogenase, long chain ACAT1 Acetyl-CoA acetyltransferase 1 ACSL1,6 Acyl-CoA synthetase long-chain family member 1,6 ELOVL4, 5, 6 ELOVL fatty acid elongase 4,5,6 LRP2, 4 Low density lipoprotein receptor related-protein 2, 4   PPARGC1B Peroxisome proliferator-activated receptor gamma, coactivator 1 betamiRNA’s targets were predicted using Diana microT-CDS (http://diana. [score:3]
Impact of miR-20a-5p and miR-142-5p on the expression of ELOVL6, gene involved in lipid metabolism. [score:3]
As seed regions are conserved between human and cattle for miR-20a-5p and miR-142-5p, putative targets for these miRNA were predicted with a high degree of accuracy based on DIANA-microT-CDS v5.0 [53]. [score:3]
Then among the eight candidate miRNAs highlighted by the RNA sequencing study, it was confirmed, by RT-qPCR on samples from 11 cows (each cow receiving each diet), that the expression of miR-20a-5p and miR-142-5p was decreased by sunflower oil supplementation. [score:3]
Sunflower oil supplementation altered the expression of miR-20a-5p and miR-142-5p. [score:3]
MiR-20a-5p also potentially targets KDM6B, a demethylase illustrating a mutual regulation of two epigenetic processes [66]. [score:3]
Predicted targets of miR-20a-5p and miR-142-5p among DEG previously identified by microarrays. [score:3]
MiR-20a-5p and miR-142-5p were transfected separately or together for 48h in BME-UV1 cells, and the expression of ELOVL6 was quantified by RT-qPCR. [score:3]
Consequently, miR-20a-5p and miR-142-5p may impact lipid metabolism in response to oil supplementation as a function of their putative targets. [score:3]
Although the response of miR-20a-5p to lipid supplementation has not yet been described, the change in the expression of miR-142-5p observed here is in line with previous reports regarding adipose tissues. [score:3]
Potential regulations through miR-20a-5p in milk fat metabolism. [score:2]
0185511.g003 Fig 3Potential regulations through miR-20a-5p in milk fat metabolism. [score:2]
0185511.g004 Fig 4Predicted binding site of miR-20a-5p, miR-142-5p and miR-1-3p in the 3’UTR of ELOVL6. [score:1]
Eight miRNAs (miR-15a-5p, miR-17-5p, miR-20a-5p, miR-33a-3p, miR-126-3p, miR-181a-5p, miR-142-5p and miR-223-3p; Supplementary S1 Table) were chosen for further study on the basis of their ranking and their function highlighted in the literature. [score:1]
To evaluate the role of miR-20a-5p and miR-142-5p, these miRNAs were over-expressed in bovine mammary epithelial cell line. [score:1]
No significant changes were obtained in cells transfected with miR-20a-5p and miR-142-5p, either separately or together (Fig 5B). [score:1]
In addition, a direct interaction between miR-20a-5p and PPARγ has been confirmed using luciferase assay in Cos-7 cells [63]. [score:1]
In order to investigate the functional role of miR-20a-5p and miR-142-5p, computational applications were used to predict their targets. [score:1]
Predicted binding site of miR-20a-5p, miR-142-5p and miR-1-3p in the 3’UTR of ELOVL6. [score:1]
We can hypothesize that the interaction predicted between miR-20a-5p and miR-142-5p in BME-UV1 cells does not occur or that the miRNAs affects the protein level. [score:1]
Putative targets of miR-20-5p and miR-142-5p among these DEG were investigated. [score:1]
Taken together, these predictions suggest a potential and crucial role for miR-20a-5p at different levels in milk fat synthesis and secretion. [score:1]
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2
[+] score: 24
Other miRNAs from this paper: bta-mir-99b, bta-mir-494
Follicular cells from follicles harboring incompetent oocytes have a molecular profile marked by an increase in PTEN expression and lower expression of bta-miR-494 and bta-miR20a, which indicates lower activity of the PI3K-Akt pathway. [score:5]
Thus, o PTEN levels are regulated by the increase in miR-494 and miR-20a expression in follicles harboring competent oocytes. [score:4]
Additionally, bta-miR-20a was recently validated as a regulator of PTEN in bovine granulosa cells [13]. [score:2]
We determined the levels of genes downstream of PI3K-Akt signaling that are related to different cellular processes (i. e., protein synthesis, DNA repair, cell cycle progression, and apoptosis), as well as the levels of the PTEN regulators bta-miR-494 and bta-miR-20a. [score:2]
We were able to identify a molecular landscape formed by the PI3K-Akt pathway in FCs consisting of lower PTEN, FOXO3a, bta-mir-494, bta-miR-20a, and BAX levels, leading to high oocyte developmental potential (Fig 4). [score:2]
Similar analysis were performed for bta-miR-20a, which was validated as a regulator of PTEN in granulosa cells [13]. [score:2]
MiR-494 and miR-20a analysis in fcs in relation to oocyte competence. [score:1]
The Ct values of bta-miR-494 and bta-miR-20a, were normalized to the geometric mean of two small reference RNAs (miR-99b and RNU1) and the relative expression was calculated using the 2 [-ΔCt] transformation. [score:1]
For miRNA-494 and miR-20a, the reverse transcription reaction was performed with 100 ng of total RNA using the miScript PCR Starter Kit (Qiagen, Venlo & Limburg, The Netherlands), according to the manufacturer’s instructions. [score:1]
Specifically, some miRNAs, such as miR-494 and miR-20a, have been shown to exert modulatory effects on PTEN levels and, thus, on genes downstream of the PI3K-Akt pathway [12, 13]. [score:1]
In the present study, we detected higher levels of the PTEN mRNA and lower levels of bta-miR-494 and bta-miR-20a, two validated modulators of PTEN, in FCs from poor oocyte quality groups, suggesting that the PI3K-Akt signaling pathway is less active in these follicles. [score:1]
Relative levels of bta-miR-494 and bta-miR-20a were significantly higher (p < 0.05) in FCs derived from follicles in which the oocytes cleaved, regardless of subsequent blastocyst generation (CNB and BL), than in follicles in which the oocytes did not cleave (NC) (Fig 2A and 2B). [score:1]
Relative levels of bta-miR-494 and bta-miR-20a in follicular cells. [score:1]
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3
[+] score: 21
Finally, miR-17-5p and miR-20a-5p, that we found to be under-expressed in the LM fraction and potentially target PTEN and STAT signaling, if down-regulated were proved to trigger cell apoptosis [68]. [score:8]
14/26s highly expressed in the HM fraction (let-7d-5p, miR-103a-3p, miR-142-3p, miR-17-5p, miR-18a-5p, miR-196a-5p, miR-20a-5p, miR-24-1-5p, miR-26a-5p, miR-301a-3p, miR-30b-5p, miR-34b-5p, miR-34c-5p, miR-378a-3p) and 7/14s highly expressed in the LM fraction (miR-10b-5p, miR-122-5p, miR-1-3p, miR-184, miR-486-5p, miR-7-5p, miR-99b-5p) were predicted to target 327 and 281 experimentally observed genes, respectively. [score:7]
Dysregulation of miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184 and miR-20a-5p was found to target three pathways (PTEN, PI3K/AKT and STAT). [score:4]
Among these pathways, “PTEN Signaling” was regulated by miR-17-5p, miR-26a-5p and miR-486-5p, “PI3K/AKT Signaling” by 122-5p and miR-184 and “STAT3 Pathway” by miR-20a-5p (Fig.   4). [score:2]
[1 to 20 of 4 sentences]
4
[+] score: 15
Other miRNAs from this paper: ssc-mir-122, ssc-mir-125b-2, ssc-mir-181b-2, ssc-mir-20a, ssc-mir-23a, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-21, ssc-mir-29c, ssc-mir-30c-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-21, bta-mir-26b, bta-mir-30d, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-30b, bta-mir-107, bta-mir-10a, bta-mir-127, bta-mir-142, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-138-2, bta-mir-17, bta-mir-181c, bta-mir-192, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-214, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-99b, ssc-mir-99b, ssc-mir-17, ssc-mir-30b, ssc-mir-199b, bta-mir-1-2, bta-mir-1-1, bta-mir-129-1, bta-mir-129-2, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-135b, bta-mir-138-1, bta-mir-143, bta-mir-144, bta-mir-146b, bta-mir-146a, bta-mir-181d, bta-mir-190a, bta-mir-199a-2, bta-mir-202, bta-mir-206, bta-mir-211, bta-mir-212, bta-mir-223, bta-mir-26a-1, bta-mir-29d, bta-mir-30f, bta-mir-338, bta-mir-33a, bta-mir-33b, bta-mir-375, bta-mir-429, bta-mir-451, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-133a-1, ssc-mir-1, ssc-mir-146b, ssc-mir-181a-1, ssc-mir-30a, bta-mir-199c, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-133b, ssc-mir-29a, ssc-mir-30d, ssc-mir-30e, ssc-mir-199a-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-10b, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-99a, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-192, ssc-mir-142, ssc-mir-127, ssc-mir-202, ssc-mir-129a, ssc-mir-455, ssc-mir-125b-1, ssc-mir-338, ssc-mir-133a-2, ssc-mir-146a, bta-mir-26c, ssc-mir-30c-1, ssc-mir-126, ssc-mir-199a-1, ssc-mir-451, ssc-let-7a-2, ssc-mir-129b, ssc-mir-429, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-132, ssc-mir-138, ssc-mir-144, ssc-mir-190a, ssc-mir-212, bta-mir-133c, ssc-mir-26b, ssc-mir-200b, ssc-mir-223, ssc-mir-375, ssc-mir-33b
Recently, Wang et al. (2016), using ovaries and testes of young Nile tilapia, showed that miR-17-5p and miR-20a were highly expressed in the ovaries and negatively regulated DMRT1 expression, suggesting that these miRNAs could induce estrogen production by inhibiting DMRT1 expression and promoting cyp19a1a expression in Nile tilapia. [score:12]
Skaftnesmo et al. (2017) explored which miRNAs regulate mRNAs during initiation of puberty, and several regulated miRNAs in the pubertal stage had earlier been associated (miR-20a, miR-25, miR-181a, miR-202, let7c/d/a, miR-125b, miR-222a/b, miR-190a) or have now been found connected (miR-2188, miR-144, miR-731, miR-8157) to the initiation of puberty. [score:3]
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5
[+] score: 9
MiR-20 and miR-106a were identified to promote the renewal of mouse spermatogonial stem cells (SSCs) at the post-transcriptional level via targeting STAT3 and Ccnd1 [19], and miR-221/222 were found to play a crucial role in maintaining the undifferentiated state of spermatogonia through repression of KIT expression [20]. [score:5]
He Z MiRNA-20 and mirna-106a regulate spermatogonial stem cell renewal at the post-transcriptional level via targeting STAT3 and Ccnd1Stem Cells. [score:4]
[1 to 20 of 2 sentences]
6
[+] score: 9
In contrary to the members of cluster 106b~25, the two polycistronic miRNAs bta-miR-20a and -92 did not follow the same expression trend. [score:3]
Additionally in SM we found not only differential expression of miR-25 on day four of pregnancy but also of two (bta-miR-20a, -92a) of the six polycistronical miRNAs encoded on cluster 17~92. [score:3]
13 miRNAs (bta-miR-25, bta-miR-93, bta-miR-106b, bta-miR-125b, bta-miR-193a-5p, bta-miR-200b, bta-miR-200c, bta-miR-221, bta-miR-2898, bta-let-7i (MC); bta-miR-20a, bta-miR-29b, bta-miR-92 (SM)) which were found to be regulated during early pregnancy using NGS resutling, in case of MC miRNAs, in separation of cyclic and pregnant animals in PCA, were validated with RT-qPCR. [score:2]
With the exception of bta-let-7i in MC and bta-miR-20a in SM, a significant correlation was confirmed for all miRNAs including the housekeeping miRNAs. [score:1]
[1 to 20 of 4 sentences]
7
[+] score: 7
Other miRNAs from this paper: ssc-mir-122, ssc-mir-135-1, ssc-mir-135-2, ssc-mir-148a, ssc-mir-19a, ssc-mir-20a, ssc-mir-224, ssc-mir-24-1, ssc-mir-323, ssc-mir-140, ssc-mir-183, ssc-mir-214, ssc-mir-27a, ssc-mir-325, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-136, ssc-mir-153, ssc-mir-18a, ssc-mir-186, ssc-mir-196a-2, ssc-mir-204, ssc-mir-21, bta-mir-18b, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-148a, bta-mir-18a, bta-mir-21, bta-mir-221, bta-mir-27a, bta-mir-27b, bta-mir-107, bta-mir-140, bta-mir-20b, bta-mir-215, bta-let-7d, bta-mir-17, bta-mir-186, bta-mir-199b, bta-mir-210, bta-mir-214, bta-mir-450a-2, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-15a, bta-mir-19a, bta-mir-204, ssc-mir-15a, ssc-mir-17, ssc-mir-199b, ssc-mir-210, ssc-mir-221, bta-mir-101-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-135a-2, bta-mir-135a-1, bta-mir-135b, bta-mir-136, bta-mir-146b, bta-mir-153-1, bta-mir-153-2, bta-mir-183, bta-mir-24-1, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-224, bta-mir-323, ssc-mir-101-1, ssc-mir-101-2, ssc-mir-133a-1, ssc-mir-450a, ssc-mir-146b, ssc-mir-215, bta-mir-1343, bta-mir-2320, bta-mir-2326, bta-mir-2366, bta-mir-2411, bta-mir-2483, bta-mir-450a-1, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-103-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-450c, ssc-mir-133a-2, ssc-let-7a-2, ssc-mir-18b, ssc-mir-1343, ssc-mir-2320, bta-mir-450b, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-2366-1, ssc-mir-2366-2, ssc-mir-2411, ssc-mir-2483
Phylogenetic trees of mir-17-mir-20 cluster among different mammalian species were constructed using MEGA4. [score:1]
0031426.g005 Figure 5 Folding secondary structure of porcine mir-17 cluster including four miRNAs (mir-17, mir-18, mir-19a and mir-20) and flanking sequences was predicted by RNAfold. [score:1]
0031426.g004 Figure 4 Folding secondary structure of porcine mir-17 cluster including four miRNAs (mir-17, mir-18, mir-19a and mir-20) and flanking sequences was predicted by RNAfold. [score:1]
The mir-17-mir-20 cluster consisted of four miRNA genes. [score:1]
mir-17 and mir-20 had closer phylogenetic and evolutionary relationship with each other (Figure 5). [score:1]
The folding secondary structure of porcine mir-17-mir-20 cluster predicted computationally by RNAfold. [score:1]
The result partially proved that mir-20 resulted from duplication of mir-17 and the history of mir-17 cluster might be closely linked to the early evolution of the mammalian lineage [38]. [score:1]
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8
[+] score: 7
Previous studies have demonstrated that the expression of MyoG is strongly correlated with miRNA (miR-20a) expression, which in turn controls cell cycle exit by targeting E2F transcription factors [68], [76], [77], [78]. [score:7]
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9
[+] score: 6
Other miRNAs from this paper: hsa-let-7f-1, hsa-let-7f-2, hsa-mir-20a, hsa-mir-21, hsa-mir-26a-1, hsa-mir-34a, hsa-mir-182, hsa-mir-210, hsa-mir-215, hsa-mir-221, hsa-mir-1-2, hsa-mir-15b, hsa-mir-122, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-141, hsa-mir-144, hsa-mir-127, hsa-mir-1-1, hsa-mir-34b, hsa-mir-34c, hsa-mir-26a-2, hsa-mir-375, hsa-mir-133b, hsa-mir-20b, hsa-mir-429, hsa-mir-451a, hsa-mir-486-1, hsa-mir-802, bta-mir-26a-2, bta-let-7f-2, bta-mir-16b, bta-mir-21, bta-mir-221, bta-mir-34b, bta-mir-127, bta-mir-15b, bta-mir-20b, bta-mir-215, bta-mir-210, bta-let-7f-1, bta-mir-122, bta-mir-34c, bta-mir-34a, bta-mir-1-2, bta-mir-1-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-141, bta-mir-144, bta-mir-16a, bta-mir-182, bta-mir-26a-1, bta-mir-375, bta-mir-429, bta-mir-451, bta-mir-486, bta-mir-2285a, bta-mir-2285d, bta-mir-2285b-1, bta-mir-2376, bta-mir-2285c, bta-mir-1388, bta-mir-3431, hsa-mir-451b, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-6119, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2285n-7, bta-mir-2285k-2, bta-mir-6529a, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2285k-5, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, hsa-mir-486-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-6529b, bta-mir-133c, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2285ae, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm, hsa-mir-6529
In recent years, the value of circulating miRNAs as diagnostic biomarkers has been shown in relation to cancer (e. g. miR-21, miR-20, miR-221 [10, 11]), cardiovascular disease (miR-1, miR-133a [12]), liver disease (miR-122 [13]) and diabetes (miR-375 and miR-34 [14]), among many other human pathologies and physiological processes including pregnancy [15]. [score:5]
Moreover, miRNAs enriched in the bovine blood cell fraction included many previously reported to be blood cell-derived in humans, such as miR-144, miR-451, let-7f, miR-26a, miR-15b, miR-20a, miR-16a, miR-16b and miR-486 [18, 25, 37, 39– 41]. [score:1]
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10
[+] score: 6
Serum levels of miR-16, miR-17, miR-20a, miR-20b, miR-26a, and miR-26b were up-regulated in an experimental sepsis condition induced by cecal ligation and puncture in mice 34, whereas over -expression of miR-21, miR-29b and miR-148a occurred in systemic lupus erythematosus 35 36. [score:6]
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11
[+] score: 3
Other miRNAs from this paper: mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-29b-1, mmu-mir-30b, mmu-mir-99a, mmu-mir-126a, mmu-mir-132, mmu-mir-141, mmu-mir-181a-2, mmu-mir-185, mmu-mir-193a, mmu-mir-199a-1, mmu-mir-200b, mmu-mir-34c, mmu-let-7d, mmu-mir-196a-1, mmu-mir-196a-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-16-1, mmu-mir-16-2, mmu-mir-20a, mmu-mir-22, mmu-mir-23a, mmu-mir-26a-1, mmu-mir-26b, mmu-mir-34a, mmu-mir-200c, mmu-mir-212, mmu-mir-181a-1, mmu-mir-26a-2, mmu-mir-29b-2, mmu-mir-199a-2, mmu-mir-199b, mmu-mir-378a, mmu-mir-451a, mmu-mir-674, mmu-mir-423, mmu-mir-146b, bta-mir-26a-2, bta-let-7f-2, bta-mir-16b, bta-mir-26b, bta-mir-99a, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-30b, bta-mir-193a, bta-let-7d, bta-mir-132, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-22, bta-mir-23a, bta-mir-29b-2, bta-mir-423, bta-let-7g, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-let-7i, bta-mir-23b, bta-mir-34c, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-34a, bta-mir-141, bta-mir-146b, bta-mir-16a, bta-mir-185, bta-mir-196a-2, bta-mir-196a-1, bta-mir-199a-2, bta-mir-212, bta-mir-26a-1, bta-mir-29b-1, bta-mir-181a-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-2284w, bta-mir-2284x, bta-mir-2284y-1, mmu-let-7j, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285t, bta-mir-2284z-2, mmu-let-7k, mmu-mir-126b, bta-mir-2284ab, bta-mir-2284ac
Among them, four miRNA (miR-29b-3p, miR-181a-5p, miR-181b-5 and miR-451a-5p) and five miRNA (miR-20a-5p, miR-23b-3p, miR-26b-5p, miR-99a-5p and miR-199a-3p) in the mouse and bovine, respectively, were expressed in the other species with moderate (over 10,000 reads) to high abundance (Table S2). [score:3]
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[+] score: 3
The miRNAs which were most abundant in plasma (Fig 3B) in our experiment are reportedly expressed at high levels in blood cells, including erythrocytes (miR-451, miR-16b), leukocytes (miR-150, miR-27a, miR-23a) and thrombocytes (miR-223, miR-20a, miR-24) and are putatively released into the plasma through apoptosis, lysis or active shedding [41, 42, 14]. [score:3]
[1 to 20 of 1 sentences]
13
[+] score: 3
The function of miR-17, especially miR-17-5p, when in combination with miR-20a and miR-106a is to inhibit monocyte proliferation, differentiation and maturation, as appeared in a review by Lindsay [39]. [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7c, hsa-let-7d, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-20a, hsa-mir-21, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-99a, hsa-mir-148a, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-181a-2, hsa-mir-181a-1, hsa-mir-125b-1, hsa-mir-143, hsa-mir-125b-2, hsa-mir-126, hsa-mir-146a, hsa-mir-155, hsa-mir-106b, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-151a, hsa-mir-450a-1, hsa-mir-452, hsa-mir-450a-2, hsa-mir-92b, hsa-mir-151b, hsa-mir-378d-2, bta-mir-26a-2, bta-let-7f-2, bta-mir-148a, bta-mir-151, bta-mir-16b, bta-mir-21, bta-mir-26b, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-92a-2, bta-let-7d, bta-mir-17, bta-mir-450a-2, bta-mir-7-3, bta-let-7f-1, bta-let-7c, bta-mir-125b-2, bta-mir-15a, bta-mir-99b, hsa-mir-450b, bta-mir-106b, bta-mir-143, bta-mir-146a, bta-mir-155, bta-mir-16a, bta-mir-26a-1, bta-mir-378-1, bta-mir-452, bta-mir-92a-1, bta-mir-92b, bta-mir-7-2, bta-mir-7-1, bta-mir-181a-1, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-450a-1, bta-mir-378-2, hsa-mir-378b, bta-mir-2284w, bta-mir-2284x, hsa-mir-378c, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, bta-mir-450b, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, hsa-mir-378j, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-378b, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-378c, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2284y-7, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, bta-mir-378d, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
Other highly expressed microRNAs previously associated with endothelial cells included miR-21, miR-378, miR-20a, miR-17, and miR-26a [Kuehbacher et al., 2007; Wang and Olson, 2009; Bonauer et al., 2010]. [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-20a, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-101-1, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-199a-1, hsa-mir-30c-2, hsa-mir-199a-2, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-125b-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-140, hsa-mir-141, hsa-mir-152, hsa-mir-191, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-149, hsa-mir-150, hsa-mir-320a, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-101-2, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-379, hsa-mir-423, hsa-mir-451a, hsa-mir-486-1, hsa-mir-496, hsa-mir-520a, hsa-mir-525, hsa-mir-518b, hsa-mir-516b-2, hsa-mir-516b-1, hsa-mir-516a-1, hsa-mir-516a-2, hsa-mir-92b, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, bta-mir-26a-2, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-16b, bta-mir-21, bta-mir-27a, bta-mir-320a-2, bta-mir-125a, bta-mir-125b-1, bta-mir-199a-1, bta-mir-31, bta-mir-140, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-191, bta-mir-192, bta-mir-22, bta-mir-23a, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-mir-150, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-23b, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-99b, hsa-mir-1249, hsa-mir-103b-1, hsa-mir-103b-2, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, bta-mir-101-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-141, bta-mir-152, bta-mir-16a, bta-mir-24-1, bta-mir-199a-2, bta-mir-223, bta-mir-26a-1, bta-mir-379, bta-mir-451, bta-mir-486, bta-mir-496, bta-mir-92a-1, bta-mir-92b, bta-mir-1249, bta-mir-320b, bta-mir-320a-1, hsa-mir-320e, hsa-mir-23c, hsa-mir-451b, bta-mir-149, hsa-mir-486-2
We detected a total of 208 miRNAs in bovine plasma (based on mean Cq < 35 across all sample pools; Fig.   4a), the most abundant of which (Fig.   4b) corresponded to miRNAs reportedly expressed at high levels in blood cells including erythrocytes (miR-451, miR-486, miR-16), leukocytes (miR-150, miR-27a, miR-23a) and thrombocytes (miR-223, miR-20a, miR-24), and which are putatively released into the plasma through apoptosis, lysis or active secretion [36– 38]. [score:3]
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[+] score: 2
Regarding the top 20 c-miRNAs, the c-miRNA profiles of both JB cattle groups were considerably similar to those of JSH cattle [24], except that in the latter, miR-15b, miR-19b, miR-20a, and miR-26a were not in the top 20 but were above 30. [score:1]
The only differences were that miR-20a was present at higher levels in the grain-fed cattle than in the grazing cattle, and that miR-29a was present at higher levels in the grazing cattle, although both miR-20a and miR-29a were within the top 25 miRNAs in both cattle groups. [score:1]
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A single bout of exhaustive cycling or rowing training for 90 days elevated plasma miR-20a, miR-21, miR-146a, miR-221, and miR-222 levels [29], whereas a single bout of cycling exercise at 70% VO [2 max] for 60 min decreased the circulating level of miR-486 immediately after the exercise [30]. [score:1]
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Notable, bta-miR-17-5p and bta-miR-20a are DE miRNAs identified in our study and show a FC < 2.0 (Table 1). [score:1]
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The top 20 miRNAs in the milk exosome were let-7b (12.7 %), miR-200c (10.9 %), miR-26a (8.8 %), let-7c (7.5 %), let-7a-5p (6.3 %), miR-30a-5p (3.1 %), miR-320a (2.7 %), miR-103 (2.5 %), miR-107 (2.2 %), let-7d (1.9 %), miR-23-3p (1.6 %), miR-191 (1.6 %), miR-23a (1.6 %), miR-20a (1.5 %), miR-1777b (1.5 %), miR-151-5p (1.4 %), miR-24-3p (1.3 %), miR-320b (1.3 %), miR-200b (1.2 %), and miR-141 (1.2 %) (Fig.   2). [score:1]
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phosphorylation of L-AAs (FDR = 7.76E-04, n = 13, such as miR-15b) was predicted to be influenced by mammary positively correlated miRNAs and phosphorylation of amino acids (FDR = 1.83E-08, n = 44, such as miR-20a) was predicted to be controlled by mammary negatively correlated miRNAs. [score:1]
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For example, the levels of miR-20a and miR-106b were positively correlated (R = 0.99) while miR-2288 and miR-671 were negatively correlated (R = -0.76). [score:1]
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-22, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-98, hsa-mir-99a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-106a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-10a, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-181a-1, hsa-mir-221, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-15b, hsa-mir-27b, hsa-mir-30b, hsa-mir-130a, hsa-mir-152, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-185, hsa-mir-193a, hsa-mir-320a, hsa-mir-200c, hsa-mir-1-1, hsa-mir-181b-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-99b, hsa-mir-130b, hsa-mir-30e, hsa-mir-363, hsa-mir-374a, hsa-mir-375, hsa-mir-378a, hsa-mir-148b, hsa-mir-331, hsa-mir-339, hsa-mir-423, hsa-mir-20b, hsa-mir-491, hsa-mir-193b, hsa-mir-181d, hsa-mir-92b, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-378d-2, bta-mir-29a, bta-let-7f-2, bta-mir-148a, bta-mir-18a, bta-mir-221, bta-mir-27a, bta-mir-30d, bta-mir-320a-2, bta-mir-99a, bta-mir-181a-2, bta-mir-27b, bta-mir-30b, bta-mir-106a, bta-mir-10a, bta-mir-15b, bta-mir-181b-2, bta-mir-193a, bta-mir-20b, bta-mir-30e, bta-mir-92a-2, bta-mir-98, bta-let-7d, bta-mir-148b, bta-mir-17, bta-mir-181c, bta-mir-191, bta-mir-200c, bta-mir-22, bta-mir-29b-2, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-10b, bta-mir-24-2, bta-mir-30a, bta-let-7a-1, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-25, bta-mir-363, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-15a, bta-mir-19a, bta-mir-19b, bta-mir-331, bta-mir-374a, bta-mir-99b, hsa-mir-374b, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, bta-mir-1-2, bta-mir-1-1, bta-mir-130a, bta-mir-130b, bta-mir-152, bta-mir-181d, bta-mir-182, bta-mir-185, bta-mir-24-1, bta-mir-193b, bta-mir-29d, bta-mir-30f, bta-mir-339a, bta-mir-374b, bta-mir-375, bta-mir-378-1, bta-mir-491, bta-mir-92a-1, bta-mir-92b, bta-mir-9-1, bta-mir-9-2, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, bta-mir-320b, bta-mir-339b, bta-mir-19b-2, bta-mir-320a-1, bta-mir-193a-2, bta-mir-378-2, hsa-mir-378b, hsa-mir-320e, hsa-mir-378c, bta-mir-148c, hsa-mir-374c, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-378j, bta-mir-378b, bta-mir-378c, bta-mir-378d, bta-mir-374c, bta-mir-148d
MiR-92a belongs to the miR-17 ~92 cluster with seven miRNAs (miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-19b, miR-20a and miR-92a) and was first described as an oncogenic miRNA cluster involved in B-cell lymphoma [61]. [score:1]
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