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12 publications mentioning bmo-mir-1a

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-1a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 44
On the basis of the evaluated luciferase activity, luc expression was shown to be strongly repressed, suggesting that bmo-miR-1a-3p might downregulate BmVMP23 expression via complementary interactions with target sites at the 3'-UTR. [score:8]
Liu et al. (2010a) were the first to report spatial expression patterns of nearly 100 miRNAs in multiple normal tissues of female and male B. mori using microarray and northern-blotting analyses, in which only 10 miRNAs were detected to express in universal tissue types of the B. mori, such as bmo-let-7 and bmo-bantam, whereas the majority were distributed exclusively or preferentially in specific tissues, such as bmo-miR-275 and bmo-miR-1. Other experimental protocols commonly used to detect miRNA expressions are polymerase chain reaction (PCR) technology, polyacryla-mide gel electrophoresis (PAGE)/northern blotting, and real-time PCR (qPCR). [score:7]
Singh et al. (2012) provided evidence that the BmNPV modulated the small -RNA -mediated defense of its host by encoding an miRNA (bmnpv-miR-1) that down-regulated the expression of the host GTP -binding nuclear protein, Ran. [score:6]
This result indicated that up-regulation of Bmo-miR-1 may lead to B. mori molting disorder. [score:4]
Yang et al. (2012) constructed expression vectors />ZEx-1-EGFP-pri-mir-1a/8/133 containing the promoter ie1, the enhancer hr5, and three corresponding pri-miRNA sequences. [score:3]
Expression of a vitelline membrane protein, BmVMP23, is repressed by bmo-miR-1a-3p in silkworm. [score:3]
Furthermore, they found a miRNA (bmo-miR-1a-3p) that matched perfectly to the 3-UTR sequence of BmVMP23 and conducted an in vitro co-transfection experiment to verify the expression relationship between bmo-miR-1a-3p and BmVMP23. [score:3]
[c] Gao predicted 87 potential bmo-miR-1 target genes based on available Bombyx mori 3'-UTR data in silico. [score:3]
Furthermore, this study revealed that the immediate-early gene targeted by bmo-miR-8 could be controlled by bmnpv-miR-1 and Ran dsRNA, resulting in increasing virus infection levels in the B. mori larvae. [score:3]
The regulation of silkworm larvae ecdysis by bmo-miR-1. [score:2]
The constructed miRNA vectors were successfully transfected into BmN cells and quantitative RT-PCR (qRT-PCR) analysis showed the relative abundances of bmo-mir-1a, bmo-mir-8, and bmo-mir-133 in BmN cells. [score:1]
In the program to figure out the intercommunication between miR-1 and B. mori molting disorder, when prepupal stage B. moris were injected with miR-1 mimics, the old epidermis was not slough off, and the formation of the internal pupal epidermis was slightly affected by the old translucent epidermis (Gao et al. 2012 ). [score:1]
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2
[+] score: 30
Stage-by-stage examinations revealed further expression patterns, such as emergence at specific time-points during embryogenesis and up-regulation of miRNA groups in late embryos (miR-1 and bantam), expression associated with stage transition between instar and molt larval stages (miR-34b), expression associated with silk gland growth and spinning activity (miR-274), continuous high expression from the spinning larval to pupal and adult stages (miR-252 and miR-31a), a coordinate expression trough in day 3 pupae of both sexes (miR-10b and miR-281), up-regulation in pupal metamorphosis of both sexes (miR-29b), and down-regulation in pupal metamorphosis of both sexes (miR-275). [score:20]
In total, 15 miRNAs were highly expressed throughout pupal and adult stages by microarray, among which 10 were common to both sexes and 9 were robustly expressed over the whole life cycle (including miR-252, miR-1, and let-7a) (Additional files 9 and 10). [score:5]
Half of the 92 miRNAs passed the filtering expression threshold, 35 were also confirmed by, and 15 (including bantam and miR-1) were robustly expressed from the embryo to adult stages (Additional files 3 and 4). [score:5]
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3
[+] score: 26
The most abundantly expressed miRNAs in B. mori are also highly conserved miRNAs e. g, miR-1, miR-8, miR-10, let-7, miR-263a, miR276a, and miR-306 and were expressed in all four stages, albeit their expression levels vary across different developmental periods. [score:8]
Differential regulation of miRNAs during silkworm development implies a role for miRNAs in diverse aspects of B. mori physiology and developmentEvolutionarily, miR-1 is highly conserved and involved in muscle development and physiology [65, 66]. [score:5]
The sole miR-1 gene in Drosophila is transcriptionally up-regulated by Twist and Mef2 during myogenesis [66]. [score:4]
Evolutionarily, miR-1 is highly conserved and involved in muscle development and physiology [65, 66]. [score:2]
In B. mori, miR-1 level is very high, miR-133 is low and miR-206 is extremely low, which suggests that miR-1 likely plays a major role in muscle development and physiology in insects. [score:2]
Notably, miR-8 and miR-1 abundance was greater by 10-fold and 6-fold, respectively, in spinning larvae than in other stages. [score:1]
It includes miR-1, the entire family of miR-2, the miR-9 family (miR-9 and miR-9b), the let-7 family (let-7a, let-7j), miR-10b, miR-31, miR-71, miR-79, miR-87, miR-98, miR-100, miR-252, miR-263a, miR-275, miR-279, miR-317 and miR-1274b (Table 3 and Figure 4a). [score:1]
The top three abundant miRNAs of the silkworm are miR-1 (30%), miR-8 (11%) and miR-306 (11%) in the feeding larval stage; miR-1 (40%), miR-8 (18%), and miR-276a (15%) in the spinning larval stage; miR-276a (27%), miR-1 (16%) and miR278 (8%) in the pupal stage; miR-263a (18%), miR276a (16%) and miR-1 (12%) in the adult stage (Additional file 2). [score:1]
In addition to miR-1, miR-133 and miR-206 are designated as muscle-specific miRNAs because of their abundance in diverse muscle tissues [38, 67]. [score:1]
Interestingly, miR-1 and miR-276a are two of the three most abundant miRNAs in all four stages. [score:1]
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4
[+] score: 24
Analysis of putative target genes exhibits that the potential target genes to 6 differentially expressed miRNA, including bmo-miR-275, bmo-miR-14, bmo-miR-1a, N-50, N-46 and N-45, were classified into response to stimulus and immune system process based on GO analysis. [score:7]
Using qRT-PCR approach, 6 up-regulated miRNAs (bmo-miR-275, N-11, N-45, N-58, N-46 and N-50) and 4 down-regulated miRNAs (bmo-miR-14, bmo-miR-1a, bmo-miR-9a and bmo-miR-274) were validated for the sequence data. [score:7]
The down-regulation of bmo-miR-1a and bmo-miR-274 was also detected in BmCPV-infected 96 h midgut. [score:4]
Bombyx mori miR-8 was identified as an anti-viral miRNA, which is suppressed by BmNPV following infection or transfection of a bmnpv-miR-1 mimic into the host cell [16]. [score:3]
MiR-1 function is muscle-related as it plays central regulatory role in myoblast proliferation and differentiation in vitro [55]– [56]. [score:1]
Here, we found bmo-miR-1a and bmo-miR-274 may also be related to viral infection. [score:1]
The first insect virus encoded miRNA (HvAV-miR-1) was reported from Heliothis virescens ascovirus (HaAV3e) [12]. [score:1]
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5
[+] score: 23
For instance, miR-1 is specifically expressed in cardiac and skeletal muscle precursor cells, and regulates cardiomyocyte proliferation in vertebrates [19]. [score:4]
In Drosophila, miRNA-1 is strongly expressed throughout the mesoderm of early embryos and subsequently in somatic, visceral, and pharyngeal muscles and the dorsal vessel, and functions in the post-mitotic growth of larval muscle [20]. [score:3]
For example, miR-1 was predominantly expressed in the body wall and fat body in males, and in the malpighian tubules in females. [score:3]
Additionally, Northern-blot results confirmed sex differences in body wall expression of 10 miRNAs (e. g., bantam, miR-1, miR-13a and miR-2a) (Table 2, Figure 1D). [score:3]
Alternative probes used with both methods confirmed substantial miR-1 expression levels in the body wall and midgut during metamorphosis, which was clearly observed in the fat body of cocoon-spinning larvae and lasted until the end of the spinning period. [score:3]
In all, only 10 miRNAs were universally distributed (including bmo-let-7 and bmo-bantam), while the majority were expressed exclusively or preferentially in specific tissue types (e. g., bmo-miR-275 and bmo-miR-1). [score:3]
miR-1 (No. [score:1]
Both microarray and analyses with alternative probes for bmo-miR281-5p, bmo-miR-10b-5p, bmo-miR-1, bmo-let-7a and bmo-miR-275 yielded reproducible results. [score:1]
The highest signal was 79,375.67 (miR-1 in female head), and the average signal was 3,228.516. [score:1]
Based on microarray analysis, 10 female-biased miRNAs were detected in the malpighian tubules (miR-286, miR-228, miR-274, miR-1, miR-252, let-7a, miR-8, bantam, miR-200b, and miR-2a), whereas miR-276-5p and miR-305 showed male-prone accumulation in malpighian tubules. [score:1]
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6
[+] score: 21
Figure 7Comparison of the secondary structures of bmo-miR-1, aga-miR-1, ame-miR-1, dme-miR-1 and dps-miR-1. Computational prediction of B. mori miRNA targetsMiRNA can regulate the protein expression of genes based on the level of complementarity between miRNA seed sequences and binding sites on target mRNA [49- 51]. [score:8]
Figure 7Comparison of the secondary structures of bmo-miR-1, aga-miR-1, ame-miR-1, dme-miR-1 and dps-miR-1. MiRNA can regulate the protein expression of genes based on the level of complementarity between miRNA seed sequences and binding sites on target mRNA [49- 51]. [score:6]
bmo-miR-1, bmo-let-7a, bmo-miR-8, bmo-miR-14, bmo-miR-276a, bmo-miR-279 were strongly expressed in all developmental stages (larva, pupa and moth). [score:4]
The results of comparisons between the hairpin structures of bmo-miR-1, aga-miR-1, ame-miR-1, dme-miR-1 and dps-miR-1 precursors are shown in Figure 7. Additional file 5 shows the secondary structures of B. mori miRNAs. [score:1]
The phylogenetic tree for the mir-1 and mir-184 miRNA family is shown in Figure 6; other detailed results are listed in Additional file 4. Figure 6Phylogenetic tree of the mir-1 family and the mir-184 family. [score:1]
The phylogenetic tree for the mir-1 and mir-184 miRNA family is shown in Figure 6; other detailed results are listed in Additional file 4. Figure 6Phylogenetic tree of the mir-1 family and the mir-184 family. [score:1]
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7
[+] score: 16
In frog embryos, miR-1 promotes muscle differentiation by targeting histone deacetylase4 (HDAC4) that acts as a transcriptional repressor in the Notch pathway [46]. [score:3]
In Drosophila, miR-1 functions in Notch pathway through targeting the Notch ligand, Delta [62]. [score:3]
For analyzing the expression patterns of bmo-miR-1, bmo-miR-13, bmo-miR-14, bmo-miR-77, bmo-miR-263a, bmo-miR-275 and U6 snRNA, real-time PCR was performed in an ABI Prism® 7300 Sequence Detection system with Quant SYBR Green PCR kit (TIANGEN, BJ) following the manufacturer's instructions. [score:3]
Other examples are miR-1, miR-133, and miR-206; all are strictly expressed in muscles and heart [43], [44], [45], [46]. [score:3]
These findings give us clues that conservative co-localization of the miR-1/miR-133 cluster and its antisense gene may exhibit their conserved functions in various species. [score:1]
We also discovered six pairs that are organized as clusters; bmo-miR-1/bmo-miR-133, bmo-let-7/bmo-miR-100, bmo-miR-12/bmo-miR-283, and bmo-miR-275/bmo-miR-305 are separated by less than 20 kb apart and in the same orientation; bmo-miR-9b overlaps with bmo-miR-79 on the opposite strand; and bmo-miR-2 is adjacent to bmo-miR-13 but on the reverse strand in a tail-to-tail orientation about some twenty basepairs away. [score:1]
Bmo-miR-8, bmo-miR-9a, and bmo-miR-263a, similar to bmo-miR-1, showed a slight elevation after diapause-broken stage (DBS) and kept a relatively constant level thereafter. [score:1]
We noticed that the bmo-miR-1/bmo-miR-133 pair is highly conserved across diverse taxa including not only insects, such as honey bee and red flour beetle (data unpublished), but also vertebrates, such as frog, chicken, mouse, and human. [score:1]
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8
[+] score: 4
BmNPV-encoded miRNAs were first identified by J. Singh et al. [84], and one of these, bmnpv-miR-1, was shown to suppress host miRNA biogenesis by regulating the exportin-5 cofactor Ran [85]. [score:4]
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9
[+] score: 3
The contrary results were found among bmo-let-7, bmo-miR-1, bmo-miR-100, bmo-miR-124, bmo-miR-137, bmo-miR-14, bmo-miR-252, bmo-miR-275, bmo-miR-305, bmo-miR-307, bmo-miR-34, and bmo-miR-279c, where the literature -based collections showed higher expression levels (Additional file 12: Table S7). [score:3]
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10
[+] score: 3
A number of evolutionarily conserved miRNAs (let-7a, mir-1, and bantam) were among the most abundant miRNAs, as demonstrated previously [8, 34- 37], whereas the majority of novel miRNAs (particularly the silkworm-specific components) were among the least abundant (Additional file 13, Figures 1C,1D), consistent with the correlation between evolutionary conservation of miRNAs and their expression levels [35, 38, 39]. [score:3]
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11
[+] score: 3
Besides, Bmo-miR-8 was identified as an anti-viral miRNA, which is suppressed by BmNPV following infection or transfection of abmnpv-miR-1 into the host cell [13]. [score:3]
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12
[+] score: 1
We observed a similar mechanism in silkworm TE-miRNAs, as we previously reported in non TE-derived miRNAs [37], that a miRNA precursor is able to yield different kinds of mature miRNA sequences (such as TE-miRNA-1 and TE-miRNA-2), and likewise different precursors produce the same miRNAs. [score:1]
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