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5 publications mentioning bmo-mir-277

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-277. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 51
In the present study, we speculated that BmNPV infection induced the expression of miR-277-5p, which led to regulate the expression of Dnmt2 gene. [score:6]
Constitutive miR-277 expression shortens lifespan [36] and plays a role as a metabolic switch controlling amino acid catabolism by targeting the metabolic pathway enzymes [37]. [score:5]
In this study, we found that miR-277-5p was significantly up-regulated in BmNPV infected silkworm larvae. [score:4]
0165865.g005 Fig 5 (a) miR-277-5p was predicted to target Dnmt2. [score:3]
Dnmt2 (NM_001043469.1) was predicted to be one of the target genes for miR-277-5p using miRNA seed region binding to the 3’UTR of Dnmt2 mRNA with mfe (minimum free energy) -20.1kcal/mol (Fig 5A). [score:3]
These results suggested that Dnmt2 was one of the target genes for miR-277-5p. [score:3]
Among the 9 differential expressed miRNA, we found several studies have reported the potential functions of miR-277. [score:3]
Target validation of miR-277-5p. [score:3]
To confirm whether miR-277-5P specifically inhibited Dnmt2, the mutated reporter was constructed. [score:3]
Bombyx mori Dnmt2 3’UTR containing the potential target -binding sequences for miR-277-5p and mutated Dnmt2 3’UTR were synthesized by GenePharma (Shanghai, China). [score:3]
Overexpression of miR-277-5p has no effects on luciferase activity in the PmirGLO-Dnmt2-mut transfected cells (Fig 5B and 5C). [score:3]
For example, miR-277 expression was induced in response to parasitization by the Glyptapanteles flavicoxis parasitoid [35]. [score:3]
Drosophila miR-277 is down regulated during the adult life, and controls branched-chain amino acid catabolism. [score:2]
We found that bmo-miR-277-5p targeted the B. mori DNA cytosine-5 methyltransferase (Dnmt2) as shown by dual-luciferase reporter assay. [score:2]
As predicted, we found that the luciferase activity decreased significantly in 293 cells co -transfected with PmirGLO-Dnmt2 and miR-277-5p mimic when compared to the negative control, which indicated that miR-277-5p can directly regulated Dnmt2. [score:2]
MiR-277-5p mimics and negative controls synthesized by GenePharma (Shanghai, China) with PmirGLO, PmirGLO-Dnmt2 and PmirGLO-Dnmt2-mut constructs were co -transfected into 293 cells using lipofectamine2000 reagent (Invitrogen) according to the manufacturer's protocol. [score:1]
Validating the interaction of miR-277-5p with Dnmt2. [score:1]
To explore whether miR-277-5p play roles in silkworm-BmNPV interaction, we investigated the target gene of miR-277-5p. [score:1]
[1 to 20 of 18 sentences]
[+] score: 17
Among these identified miRNAs, we found 12 pairs of miRNAs and miRNA*s. revealed that some B. mori miRNA genes were expressed only during specific stages, indicating that B. mori miRNA genes (e. g., bmo-miR-277) have developmentally regulated patterns of expression. [score:7]
bmo-miR-277 was expressed only in moth and not detected in larva and pupa; the precursor of bmo-miR-277 was also detected [see Figure S1 of Additional file 1]. [score:3]
For example, bmo-miR-277 was expressed only during the moth stage. [score:3]
Figure 1 displayed the result of analysis for bmo-miR-277. [score:1]
Members of the bantam, mir-71, mir-275, mir-277, mir-279 miRNA families have a high degree of similarity in their precursor sequences. [score:1]
bmo-miR-279 and other mir-279 precursor sequences have sequence similarities of >55%; bmo-miR-277 precursors share >56% sequence similarity; bmo-miR-27 precursors share >57% sequence similarity; bmo-miR-71 precursors share >59% sequence similarity; and bmo-bantam precursors share >60% sequence similarity. [score:1]
We designated these sequences bmo-miR-13a*, bmo-miR-14, bmo-miR-46, bmo-miR-46*, bmo-miR-71, bmo-miR-277 and bmo-bantam. [score:1]
[1 to 20 of 7 sentences]
[+] score: 13
Expression profile of specific miRNAs differs between Drosophila, mosquito and silkwormMicroRNA, miR-277 expression has been detected only in Drosophila adults [20, 24]. [score:5]
Another miRNA, miR-277, was reported to be specifically expressed in the moth stage [33]. [score:3]
MicroRNA, miR-277 expression has been detected only in Drosophila adults [20, 24]. [score:3]
We detected miR-277 in all four stages. [score:1]
In contrast, miR-277 is present in the silkmoth at a level much lower than in pupae and spinning larvae (Table 3). [score:1]
[1 to 20 of 5 sentences]
[+] score: 7
Using northern blotting analysis, He et al. (2008) revealed that some B. mori miRNAs (e. g., bmo-miR-277) were expressed only during specific stages, indicating that these miRNAs have regulatory patterns of developmental expression. [score:7]
[1 to 20 of 1 sentences]
[+] score: 7
Four miRNAs (miR-29b, miR-34b, miR-277, and miR-285) were significantly up-regulated from the spinning larvae to adult stages, and nine (miR-305, miR-275, miR-289, miR-307-3p, miR-274, miR-286, miR-87, miR-315, and miR-92) were significantly down-regulated during this time-course (Additional file 12). [score:7]
[1 to 20 of 1 sentences]