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8 publications mentioning dme-mir-989

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-989. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

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[+] score: 206
GO term analysis of predicted miR-989 targets miR-989 target lists were obtained from TargetScanFly [22], TargetScanFly ORF [3], MinoTar [3] and miRNA. [score:9]
predicted miR-989 targets miR-989 target predictions were obtained from TargetScanFly [22], TargetScanFly ORF [3], MinoTar [3] and miRNA. [score:9]
Because the border cells are only a small fraction of the somatic cells, we have not attempted to validate upregulation of candidate targets in the miR-989 mutant border cells by monitoring target RNA levels. [score:8]
miR-989 target predictions were obtained from TargetScanFly [22], TargetScanFly ORF [3], MinoTar [3] and miRNA. [score:7]
miR-989 target lists were obtained from TargetScanFly [22], TargetScanFly ORF [3], MinoTar [3] and miRNA. [score:7]
il/), querying all Drosophila ontologies using the miR-989 target predictions as target set and all Drosophila genes as a background set. [score:5]
Transgenic expression of miR-989 in border cells of sibling mosaics partially suppressed the delayed border cell phenotype. [score:5]
A–D Expression of miR-989 in border cells in a miR-989 mutant background suppressed the delayed border cell migration phenotype. [score:5]
This sensor encodes eGFP expressed ubiquitously under control of a tubulin promoter, followed by a 3' UTR containing two target sites for miR-989. [score:5]
Predicted miR-989 targets associated with the GO terms ‘cell migration’ and ‘regulation of cell projections’ are obvious candidates whose de-regulation may cause border cell migration phenotypes. [score:5]
The border cell migration defect can be rescued by transgenic miR-989 expressionTo confirm that lack of miR-989 was responsible for the border cell migration defects described above, we expressed miR-989 from an UAS transgene. [score:5]
miR-989 is expressed in the somatic cells throughout egg chamber development. [score:4]
The border cell migration phenotype was rescued by transgenic expression of miR-989. [score:3]
It shows GO term enrichment of predicted miR-989 targets (color coded). [score:3]
Border cells transgenically expressing miR-989 in a miR-989 mutant background most often reached the oocyte by this stage. [score:3]
We show that border cell migration is delayed in miR-989 mutant egg chambers, and that this phenotype can be rescued by transgenic expression of the miRNA. [score:3]
GO term analysis of predicted miR-989 targets. [score:3]
A Genetic mosaics: wild type cells are labelled by nuclear GFP (upper panel) while miR-989 mutant cells do not express GFP. [score:3]
predicted miR-989 targets. [score:3]
Expression with the Slbo-Gal4 driver did not rescue border cell migration in a miR-989 [KO] mutant background. [score:3]
GFP -negative border cell clusters lacking miR-989 were delayed in their migration (Fig. 5A, C, D), whereas GFP -positive border cell clusters with transgenic miR-989 expression migrated almost normally (Fig. 5B, C, D; p<0.001). [score:3]
In the miR-989 mutant background, a homogenous GFP signal was also observed in all somatic cells, including the border cell cluster while GFP expression in the germ line cells was unchanged (Fig. 3B, D, arrows). [score:3]
Somatic cells, where miR-989 is expressed, comprise a small fraction of the total tissue. [score:3]
As a first step to address this question, we generated transgenic flies that express a miRNA sensor [21] for miR-989. [score:3]
miR-989 could act on multiple targets including those affecting the processes discussed above to permit normal border cell migration. [score:3]
Presence of GFP indicates transgenic miR-989 expression. [score:3]
Predicted miR-989 targets associated with the GO term ‘cell adhesion’ may be particularly interesting. [score:3]
To confirm that lack of miR-989 was responsible for the border cell migration defects described above, we expressed miR-989 from an UAS transgene. [score:3]
The border cell migration defect can be rescued by transgenic miR-989 expression. [score:3]
Table S1 GO terms enriched among the predicted miR-989 targets. [score:3]
Stage 8 (A, B) and Stage 10A (C, D) eggchambers expressing a miR-989 GFP sensor in a wild type (A, C) or in a miR-989 mutant background (B, D). [score:3]
Figure S1 GO term analysis of predicted miR-989 targets. [score:3]
A Example of a mosaic egg chamber in which miR-989 and GFP were expressed in border cells and border cell migration was normal. [score:3]
miR-989 is required in somatic cells for efficient border cell migrationThe predominant pattern of follicle cell expression suggested that miR-989 activity may be required in the follicle cells or the border cells to promote normal border cell migration. [score:3]
0067075.g005 Figure 5The border cell migration phenotype was rescued by transgenic expression of miR-989. [score:3]
Table S1 shows an annotated list of the GO terms that are enriched among predicted miR-989 targets. [score:3]
The predominant pattern of follicle cell expression suggested that miR-989 activity may be required in the follicle cells or the border cells to promote normal border cell migration. [score:3]
B Example of a mosaic egg chamber in which miR-989 and GFP were not expressed in border cells, and border cell migration was drastically delayed. [score:3]
In contrast, miR-989 expression under the control of a heat-shock inducible actin-flip-out-Gal4 cassette was able to rescue. [score:3]
In this report, we identify the miRNA miR-989 as a regulator of border cell migration. [score:2]
miR-989 activity in somatic cellsBorder cell migration depends on guidance signals from germ-line cells, signal interpretation by the border cells to produce directed migration and interaction between the two cell types to allow movement of the border cell cluster on and between the nurse cells [5], [6], [7]. [score:2]
miR-989 activity in somatic cells. [score:1]
Comparing the Slbo-Gal4 and flip-out clonal rescue results suggests that miR-989 may be required from the onset of border cell migration. [score:1]
In miR-989 mutant egg chambers, border cells were frequently delayed relative to the migrating main body follicular epithelium. [score:1]
However, follicle cells were positively labelled by the sensor in the absence of miR-989. [score:1]
In the stage 10A egg chamber on the left all somatic cells are wild-type (GFP positive), while all germ line cells are mutant for miR-989 (GFP negative). [score:1]
We also observed egg chambers in which the border cell cluster was partially wild type and partially mutant for miR-989 while germ line was wild-type (Fig. 4B, C). [score:1]
For the rescue analysis, third instar larvae of the genotype hsFLP/+; miR-989 [KO]/miR-989 [KO]; pT-989@Fb/AFG,10xGFP were heat-shocked at 37°C for 1–2h and ovaries were then dissected from 2–3d old adult females for analysis. [score:1]
In this light, it is interesting to note that loss of miR-989 from a subset of border cells is sufficient to cause border cell migration delays. [score:1]
Ovaries derived from young females bearing the miR-989 [KO] allele in trans to a genomic deficiency (Df(2R)Exel7130) uncovering the miR-989 locus proved to be morphologically normal (not shown). [score:1]
Deletion of the miR-989 gene was confirmed by PCR on genomic DNA (not shown). [score:1]
To generate the miR-989 sensor, oligos encoding two perfect miR-989 binding sites were annealed and cloned into the tub>eGFP transgene [21]. [score:1]
These results provide evidence that miR-989 is required in somatic cells for normal migration, but dispensable in the germ-line. [score:1]
0067075.g004 Figure 4Clonal analysis demonstrates a somatic requirement for miR-989 for normal border cell migration. [score:1]
In S10B egg chambers lacking miR-989, border cells were frequently found within the nurse cell compartment. [score:1]
Wild type border cells migrated normally when the germ line was mutant for miR-989. [score:1]
Deep sequencing of an ovarian small RNA library identified miR-989 as the most abundant miRNA species in the Drosophila ovary, constituting 15.9% of all annotated sequencing reads [18]. [score:1]
miR-989 is required in somatic cells for efficient border cell migration. [score:1]
In contrast, miR-989 and GFP negative border cells in sibling mosaics were strongly delayed. [score:1]
In the stage 10A egg chamber shown on the right all germ line cells are wild type (GFP positive) while all somatic cells are mutant for miR-989 (GFP negative). [score:1]
These observations suggested that miR-989 is required for some aspect of border cell migration towards the oocyte. [score:1]
This demonstrates that loss of miR-989 was responsible for the delayed border cell migration phenotype in the miR-989 mutant egg chambers. [score:1]
Border cell migration is delayed in miR-989 mutant egg chambers. [score:1]
We observed that border cell migration was frequently delayed in miR-989 [KO] / Df(2R)Exel7130 ovaries compared to controls and quantitated this phenotype during two stages of egg chamber development (Fig 2). [score:1]
Clonal analysis demonstrates a somatic requirement for miR-989 for normal border cell migration. [score:1]
0067075.g002 Figure 2Border cell migration is delayed in miR-989 mutant egg chambers. [score:1]
The UAS-miR-989 transgene was cloned into pUAST. [score:1]
This suggests that lack of miR-989 in just some border cells is sufficient to cause delays affecting the entire cluster. [score:1]
We therefore asked whether miR-989 was acting in the somatic cells or germ line cells of the ovary. [score:1]
Mosaic egg chambers in which border cells were mutant for miR-989 were strongly delayed in their migration. [score:1]
We observed a population of border cell clusters that were partially wild-type and partially mutant for miR-989. [score:1]
To test whether miR-989 has an important function during oogenesis, we generated a deletion allele (designated miR-989 [KO]) by ends-out homologous recombination [19], [20]. [score:1]
For mosaic analysis (Fig. 4), males bearing a recombinant FRT42A miR-989 [KO] chromosome were crossed to hsFLP; FRT42A ubiGFPnls virgins and offspring third instar larvae were heat-shocked for 1-2h at 37°C. [score:1]
Frequently, border cells had not reached the oocyte by this stage in miR-989 mutant egg chambers. [score:1]
This suggests that miR-989 is predominantly active in the somatic follicle cells. [score:1]
In contrast, migration was delayed when somatic cells including the border cell cluster were mutant for miR-989 but the germ line was wild-type (Fig. 4B, right panel). [score:1]
Moreover, we demonstrate that miR-989 is active in the somatic cells of the egg chamber and required in border cells for efficient migration. [score:1]
Migration was strongly delayed if the border cells were mutant, but not if the germ line cells lacked miR-989. [score:1]
In other words, the presence of wild-type border cells cannot compensate for the lack of miR-989 in some cells. [score:1]
In contrast, the border cell cluster lagged behind the follicular epithelium in homozygous miR-989 [KO] egg chambers (p<0.001 in comparison to the heterozygous control). [score:1]
Conversely, miR-989 mutant border cells were delayed when the germ line was wild type. [score:1]
We found that wild-type border cells migrated normally when the germ line cells lacked miR-989 (Fig. 4A, left panel). [score:1]
The miR-989 [KO] allele was generated using pRMCE as described [19], [20]. [score:1]
Over 1/3 of border cell clusters derived from miR-989 mutant females were delayed. [score:1]
To test this idea, we generated mosaic egg chambers that were partially wild-type and partially mutant for miR-989 and scored the migratory behaviour of the border cells. [score:1]
Border cell migration was delayed in a statistically significant manner (p<0.001) when all cells in the border cell cluster were mutant for miR-989, but the germ cells were wild-type. [score:1]
We did not observe a statistically significant delay in egg chambers in which the border cells were wild-type but the germline was mutant for miR-989. [score:1]
However, we do not exclude the possibility that loss of miR-989 from other somatic cells might contribute to the border cell migration phenotype. [score:1]
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[+] score: 42
Other miRNAs from this paper: hsa-mir-33a, hsa-mir-92a-1, hsa-mir-92a-2, dme-mir-1, dme-mir-8, dme-mir-11, hsa-mir-34a, hsa-mir-210, dme-mir-184, dme-mir-275, dme-mir-92a, dme-mir-276a, dme-mir-277, dme-mir-33, dme-mir-281-1, dme-mir-281-2, dme-mir-34, dme-mir-276b, dme-mir-210, dme-mir-92b, dme-bantam, dme-mir-309, dme-mir-317, hsa-mir-1-2, hsa-mir-184, hsa-mir-190a, hsa-mir-1-1, hsa-mir-34b, hsa-mir-34c, aga-bantam, aga-mir-1, aga-mir-184, aga-mir-210, aga-mir-275, aga-mir-276, aga-mir-277, aga-mir-281, aga-mir-317, aga-mir-8, aga-mir-92a, aga-mir-92b, hsa-mir-92b, hsa-mir-33b, hsa-mir-190b, dme-mir-190, dme-mir-957, dme-mir-970, dme-mir-980, dme-mir-981, dme-mir-927, dme-mir-252, dme-mir-1000, aga-mir-1174, aga-mir-1175, aga-mir-34, aga-mir-989, aga-mir-11, aga-mir-981, aga-mir-1889, aga-mir-1890, aga-mir-1891, aga-mir-190, aga-mir-927, aga-mir-970, aga-mir-957, aga-mir-1000, aga-mir-309, cqu-mir-1174, cqu-mir-281-1, cqu-mir-1, cqu-mir-275, cqu-mir-957, cqu-mir-277, cqu-mir-252-1, cqu-mir-970, cqu-mir-317-1, cqu-mir-981, cqu-mir-989, cqu-mir-1175, cqu-mir-276-1, cqu-mir-276-2, cqu-mir-276-3, cqu-mir-210, cqu-mir-92, cqu-mir-190-2, cqu-mir-190-1, cqu-mir-1000, cqu-mir-11, cqu-mir-8, cqu-bantam, cqu-mir-1891, cqu-mir-184, cqu-mir-1890, cqu-mir-980, cqu-mir-33, cqu-mir-2951, cqu-mir-2941-1, cqu-mir-2941-2, cqu-mir-2952, cqu-mir-1889, cqu-mir-309, cqu-mir-252-2, cqu-mir-281-2, cqu-mir-317-2, aga-mir-2944a-1, aga-mir-2944a-2, aga-mir-2944b, aga-mir-2945, aga-mir-33, aga-mir-980
The targets of miR-989 and miR-92 in mosquitoes are not yet known; however, several studies have examined expression of these miRNAs during development. [score:6]
quinquefasciatus mosquitoes to age- and sex-matched WNV-NY99 infected mosquitoes revealed that the majority of miRNAs were unaffected; however, we observed 2.8 fold downregulation of miR-989 following WNV-NY99 infection (Figure 3B; Additional file 1, Figure S1). [score:4]
Given the dysregulation of miR-989 and miR-92 during WNV infection, it is interesting to speculate that the targets of these miRNAs may play roles in mediating flavivirus infection in the mosquito host. [score:4]
We found miR-989, a female-specific miRNA in Anopheles and Aedes mosquitoes, to be downregulated in WNV-infected Cx. [score:4]
gambiae miRNAs, miR-34, miR-1174, miR-1175, and miR-989, show changes in expression during Plasmodium infection [11]. [score:3]
Notably, this pattern of miRNA expression for miR-989 and miR-92 is also found in deep sequencing reads of WNV-infected Cx. [score:3]
aegypti, miR-989 expression is restricted to female mosquitoes and found predominantly in the ovaries [10, 11]. [score:3]
Differences in miR-989 and miR-92 expression levels are highlighted. [score:3]
Two miRNAs, miR-92 and miR-989, showed significant changes in expression levels following WNV infection. [score:3]
Culex miR-989 and miR-92 expression levels are altered during flavivirus infection. [score:3]
Five miRNAs, miR-1, miR-317, miR-277, miR-989, and miR-92 were sequenced >120 times and were readily detectable in total RNA isolated from Cx. [score:1]
The D. melanogaster miR-989 precursor, for example, has 99 nt of intervening sequence between the miRNA and miRNA* [18]. [score:1]
aegypti mosquitoes; miR-989 is also present in the midgut while miR-92 is present in Ae. [score:1]
quinquefasciatus since (i) miR-11 and miR-989 are located on the plus strand in An. [score:1]
miR-11 and miR-989 map to the plus strand in the Ae. [score:1]
We additionally investigated the effects of flavivirus infection on miRNA expression and found that miR-92 and miR-989 are significantly changed in response to WNV infection. [score:1]
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[+] score: 7
However, we have shown that miR-x2 (miR-989) is predominantly expressed in ovaries in both An. [score:3]
Winter and colleagues state that miR-989 (miR-x2) is expressed only in midguts of An. [score:3]
gambiae miR-996 and miR-989, respectively [42] and homologs of these two miRNAs are found in the expanded list of Drosophila miRNAs [40, 41]. [score:1]
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[+] score: 4
Indeed, the 3’ arm of mir-989 is highly expressed in ovaries (figure 2). [score:3]
Recently, mir-989 has been discovered to be involved in cell migration in the ovary [50]. [score:1]
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[+] score: 3
The AMP expression phenotype for select miRNAs (miR-34, miR-92a, miR-9a and miR-989) is shown (Fig 1D and 1E). [score:3]
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[+] score: 2
However mIR-989 and aga-mir-10361b, that were ovary and testes-enriched in the adult respectively, were similarly enriched in the immature larval gonad of each sex (Additional file 7: Table S4), indicating that each microRNA must play an early role in the formation of the gonad. [score:1]
Validating our approach, microRNAs with known roles in oogenesis such as miR-989 were heavily ovary-enriched in our analysis [20, 46]. [score:1]
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[+] score: 1
Other miRNAs from this paper: dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-9a, dme-mir-10, dme-mir-12, dme-mir-13a, dme-mir-13b-1, dme-mir-13b-2, dme-mir-276a, dme-mir-133, dme-mir-276b, dme-mir-210, dme-mir-31b, dme-mir-9c, dme-mir-306, dme-mir-9b, dme-mir-31a, dme-mir-309, dme-mir-316, dme-mir-317, dme-mir-2c, ame-mir-12, ame-mir-133, ame-mir-210, ame-mir-276, ame-mir-2-1, ame-mir-2-2, ame-mir-317, ame-mir-9a, ame-mir-9b, bmo-mir-9a, bmo-mir-10, bmo-mir-276, bmo-mir-31, bmo-mir-71, ame-mir-10, ame-mir-137, ame-mir-13a, ame-mir-2-3, ame-mir-29b, ame-mir-31a, ame-mir-375, ame-mir-71, ame-mir-932, dme-mir-193, dme-mir-375, dme-mir-932, dme-mir-970, dme-mir-971, dme-mir-137, dme-mir-1006, dme-mir-1007, bmo-mir-2a-1, bmo-mir-2a-2, bmo-mir-2b, bmo-mir-13a, bmo-mir-13b, bmo-mir-133, bmo-mir-210, bmo-mir-317, tca-mir-2-3, tca-mir-2-1, tca-mir-2-2, tca-mir-10, tca-mir-12, tca-mir-13a, tca-mir-13b, tca-mir-31, tca-mir-71, tca-mir-133, tca-mir-137, tca-mir-210, tca-mir-276, tca-mir-317, tca-mir-932, tca-mir-9b, bmo-mir-12, bmo-mir-137, bmo-mir-932, bmo-mir-9b, tca-mir-9a, tca-mir-970, ame-mir-13b, ame-mir-1006, ame-mir-316, bmo-mir-970, lmi-mir-276, lmi-mir-210, lmi-mir-10, lmi-mir-9a, bmo-mir-9c, bmo-mir-306a, bmo-mir-989a, bmo-mir-316, bmo-mir-1175, bmo-mir-9d, bmo-mir-750, bmo-mir-375, bmo-mir-306b, api-mir-137, api-mir-10, api-mir-276, api-mir-13a, api-mir-210, api-mir-29, api-mir-2a, api-mir-2b, api-mir-2c, api-mir-316, api-mir-317, api-mir-71, api-mir-971, api-mir-9a, api-mir-9b, api-mir-306, api-mir-3049, bmo-mir-989b, ame-mir-1175, ame-mir-193, ame-mir-989, ame-mir-3049, ame-mir-971, ame-mir-3770, ame-mir-9c, ame-mir-306, ame-mir-750, tca-mir-9c, tca-mir-316, tca-mir-9d, tca-mir-309a, tca-mir-3049, tca-mir-375, tca-mir-29, tca-mir-1175, tca-mir-750, tca-mir-989, tca-mir-309b, tca-mir-193, tca-mir-6012, tca-mir-9e, ame-mir-6037, ame-mir-6012, ame-mir-2b, tca-mir-309c, tca-mir-971b
Examination of the miRNAs only found in the four endopterygotes (Fig. 6) indicates that MIR-989 family is present in all them, MIR-1006 in A. mellifera, B. mori and D. melanogaster, and MIR-1007 in A. mellifera and D. melanogaster. [score:1]
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[+] score: 1
Other miRNAs from this paper: dme-mir-14, dme-mir-34, dme-mir-9c
Quantification was done relative to miR-989 and normalized to heterozygous sibling ovaries. [score:1]
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