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16 publications mentioning hsa-mir-1248

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-1248. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 122
This analysis showed that 1740 mRNAs were downregulated and 1770 mRNAs were upregulated by miR-1248 overexpression (Suppl. [score:9]
Two of these potential targets, GAPVD1 and CDK2, we validated were downregulated in miR-1248 overexpressing cells (Figure 6C). [score:8]
In addition, the mRNAs that were significantly changed in miR-1248 overexpressing cells were imputed into Ingenuity to obtain a miR-1248 putative target list and compared with targets for miR-1248 in TargetScan. [score:8]
Given that the presence of low-grade systemic chronic inflammation is associated with the development and progression of age-related diseases and conditions, it is interesting to speculate that miR-151a-3p, miR-181a-5p and miR-1248 may be important for regulating inflammatory processes and inhibiting inflammation -associated aging in young individuals. [score:7]
The top canonical pathways that were downregulated in miR-1248 overexpressing cells are shown (Figure 6D). [score:6]
Interestingly, DNA repair pathways are upregulated in miR-1248 overexpressing cells (Suppl. [score:6]
Several cytokine and inflammatory -associated pathways, including NF-κB, are downregulated in miR-1248 overexpressing cells (Figure 6D). [score:6]
Therefore, we overexpressed a miR-1248 mimic in HeLa cells and performed microarray analysis in order to determine which genes and pathways are differentially changed by miR-1248 overexpression (Figure 6). [score:5]
To understand more thoroughly how these miRNAs may contribute to the aging process, we examined several mRNA targets of miR-151a-3p, miR-181a-5p and miR-1248 as predicted using TargetScan 6.2. [score:5]
Consistent with this idea, we found that miR-1248 regulates genes involved in various cytokine pathways and specifically modulates the pro-inflammatory cytokines IL-6 and IL-8. In addition, accumulating data indicate that miR-181a-1 targets several inflammatory cytokines including the mRNAs that encode IL-1β, IL-1α, IL-6, IL-8, IL-10, TNFα, TGFβ, FGF2, HMGB1 and LIF [27- 29]. [score:4]
In order to better understand the potential role miR-1248 may play in aging, we performed a genome wide microarray and found that miR-1248 regulates the expression of genes that play a role in cytokine and inflammatory pathways, as well as, DNA repair pathways. [score:4]
miR-181a, miR-1248 and miR-3607 were significantly downregulated in serum from old individuals using Poisson regression (miR-181a-1 P=0.03, miR-3607 P=0.01, miR-1248 P=4.9×10 [−9]). [score:4]
Given that there is very little known about miR-1248, we sought to determine the potential targets and pathways regulated by this miRNA. [score:4]
Pathway and Target analysis for miR-1248. [score:3]
Based on our sequencing data, we found differential expression of three miRNAs: miR-181a-1, miR-1248 and miR-3607 with human age. [score:3]
We identified 115, 626 and 265 mRNAs that were predicted to be targeted by miR-151a-3p, miR-181a and miR-1248, respectively (Figure 5A). [score:3]
This analysis suggests that miR-151a-3p, miR-181a-5p and miR-1248 may target similar, yet distinct signaling pathways important for the aging process. [score:3]
Furthermore, the miR-1248 mimic that we have used for overexpression is biotin labeled. [score:3]
To validate the microarray results, total RNA from the same samples from above (Con-miR and miR-1248 overexpressing) were reverse transcribed using random hexamers and SSII reverse transcriptase (Invitrogen) followed by RT-qPCR using SYBR green PCR master mix (Life Technologies). [score:3]
Figure 6(A) HeLa cells transfected with Con-miR or miR-1248 were analyzed 48 hrs after transfection for miR-1248 expression using real-time RT-PCR. [score:3]
We previously found that miR-1248 expression was decreased in our miRNA screen in PBMCs [14]. [score:3]
In our validation experiments in the larger cohort, we found a significant decrease in expression of miR-151a-3p, miR-181a-5p, and miR-1248. [score:3]
We further validated by real-time RT-PCR that indeed these cytokines are decreased in abundance in miR-1248 overexpressing cells (Figure 6C). [score:3]
All canonical pathways regulated by miR-1248 are shown in the heat map in Supplemental Figure S1. [score:2]
Although miR-1248 is likely to be involved in various other cellular pathways, these data suggest that decreased serum miR-1248 may potentiate inflammation and DNA damage that occurs with age. [score:1]
Figure 1 (A) Frequency of miRNA reads per individual using small RNA NGS from 11 young (~30 yr) and 11 old (~64 yr) individuals for miR-181a-1, miR-3607, miR-1248, miR-151a and miR-151b. [score:1]
There have been very few published reports on miR-1248. [score:1]
We found that CDK2, IL6 and IL8 mRNAs were enriched in our biotin miR-1248 pulldowns; whereas GAVD1 mRNA was not (Figure 6F). [score:1]
Three serum miRNAs were significantly decreased in old individuals: miR-151a-3p, miR-181a-5p and miR-1248. [score:1]
We found significantly lower levels of miR-151-5p and miR-1248 and a close to significant (p=0.09) decrease in miR-181a-5p levels with monkey age (Figure 4). [score:1]
Importantly, miR-181a-1, miR-1248 and miR-3607 were significantly lower in old individuals (Figure 1A). [score:1]
Immunoprecipitation of mRNAs associated with biotinylated-miR-1248. [score:1]
miR-181a-5p and miR-1248 were significantly decreased in old individuals (Figure 3). [score:1]
HeLa cells were transfected with 20 nM biotinylated miR-1248 (miR-1248) or a negative control, biotinylated C. elegans miR-67 (Con-miR) using Lipofectamine 2000. [score:1]
Figure S1), suggesting that decreased levels of miR-1248 with age may influence the impaired DNA repair capacity that is observed in older individuals [32]. [score:1]
HeLa cells were transfected with 20 nM biotinylated miRIDIAN miR-1248 mimic (Thermo Scientific) or a negative control, biotinylated miRIDIAN C. elegans miR-67 (here named Con-miR) using Lipofectamine 2000. [score:1]
These data indicate that miR-1248 can bind to CDK2, IL6 and IL8 mRNAs. [score:1]
Therefore, we performed biotin-labeled precipitations followed by RNA isolation and RT-qPCR to identify mRNAs bound to miR-1248. [score:1]
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2
[+] score: 24
Other miRNAs from this paper: hsa-mir-1-2, hsa-mir-1-1
There were 2877 target genes identified from 16 P. acnes small RNA fragments, 35 target genes identified from three EBOV miRNAs, and 160 target genes identified from the human miR-1248 family. [score:7]
using small RNA fragmentsTwenty small RNA fragments, including human miR-1248, were used as queries in the TargetScan human database to identify potential target genes for these small RNA fragments. [score:5]
Twenty small RNA fragments, including human miR-1248, were used as queries in the TargetScan human database to identify potential target genes for these small RNA fragments. [score:5]
This segment was also the target of the seed region of human miR-1248 (UCUUCC). [score:3]
As a result, the three groups of gene lists were separated into EBOV miRNA, P. acnes short RNA sequences, and human miR-1248 based on queries in miRBase. [score:1]
The newly identified RNA sequences are listed in Table 1. The RNA sequence CCUUCUU of PA-miR-2-3p-5 was found to be identical to the seed region of the human miR-1248 family. [score:1]
Notably, cells transfected with PA-miR-2-3p-5 and EBV-miR-2-3p showed similar transcript levels as cells transfected with human miR-1248 (Fig. 3). [score:1]
The cells were cotransfected with human miR-1248 plus PA-miR-2-3p-5, human miR-1248 plus EBV-miR-2-3p; or PA-miR-2-3p-5 plus EBV-miR-2-3p. [score:1]
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3
[+] score: 18
young) Olivieri et al. Plasma ABI TaqMan miRNA PCR array (365 miRNAs); qRT-PCR (validation) 11 20, 80, and 100 year olds; validation (only miR-21) in 111 healthy adults aged 20-105 (profiling cohort) and in 34 patients (mean, 87 years) with cardiovascular disease and 15 healthy centenarian offspring (mean, 72 years) 46 miRNAs downregulated, 12 up- then downregulated, 5 upregulated in profiling cohort; one miRNA (miR-21) downregulated in validation cohort Noren Hooten et al. Serum Deep sequencing and qRT-PCR 20 young (mean, 30.1 years) and 20 old (mean, 64.2 years) individuals 3 miRNAs downregulated (miR-151a-5p, miR-181a-5p and miR-1248) PBMCs, peripheral blood mononuclear cells. [score:18]
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4
[+] score: 7
Panganiban et al. revealed an up-regulation of miRNA-1248, which targets IL-5, in the serum of 10 asthmatic patients compared to control subjects [21]. [score:5]
Differential microRNA epression in asthma and the role of miR-1248 in regulation of IL-5. American journal of clinical and experimental immunology. [score:2]
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5
[+] score: 6
Other miRNAs from this paper: hsa-mir-3658
MIR1248 has 154 target genes and is upregulated in SK-N-SH and K562. [score:6]
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6
[+] score: 5
The majority of miRNAs (76%) showed high expression level (10 AC 100), 13 miRNAs (16%) had AC lower than 10, whereas 6 miRNAs-MIR34B, MIR34C, MIR191, MIR223, MIR1248, and MIR1905C-showed very high expression levels (AC≥100) in stallion sperm (Table S7). [score:5]
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7
[+] score: 5
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-21, hsa-mir-22, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-27a, hsa-mir-30a, hsa-mir-31, hsa-mir-98, hsa-mir-99a, hsa-mir-101-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-197, hsa-mir-199a-1, hsa-mir-208a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-10a, hsa-mir-10b, hsa-mir-34a, hsa-mir-187, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-203a, hsa-mir-211, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-mir-224, hsa-mir-200b, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-122, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-125b-1, hsa-mir-128-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-138-2, hsa-mir-140, hsa-mir-142, hsa-mir-143, hsa-mir-144, hsa-mir-145, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-126, hsa-mir-138-1, hsa-mir-146a, hsa-mir-200c, hsa-mir-155, hsa-mir-128-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-101-2, hsa-mir-219a-2, hsa-mir-34b, hsa-mir-34c, hsa-mir-99b, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-375, hsa-mir-328, hsa-mir-337, hsa-mir-338, hsa-mir-339, hsa-mir-384, hsa-mir-424, hsa-mir-429, hsa-mir-449a, hsa-mir-485, hsa-mir-146b, hsa-mir-494, hsa-mir-497, hsa-mir-498, hsa-mir-520a, hsa-mir-518f, hsa-mir-499a, hsa-mir-509-1, hsa-mir-574, hsa-mir-582, hsa-mir-606, hsa-mir-629, hsa-mir-449b, hsa-mir-449c, hsa-mir-509-2, hsa-mir-874, hsa-mir-744, hsa-mir-208b, hsa-mir-509-3, hsa-mir-1246, hsa-mir-219b, hsa-mir-203b, hsa-mir-499b
MiRNA expression was also analyzed in exhaled breath condensates from asthmatic patients and control subjects, showing decreased expression of several miRNAs in asthma (miR-1248, let-7a, miR-155, miR-21, miR-328, and miR-133a) [23]. [score:5]
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8
[+] score: 3
miRNAs selected for validation studies spanned a range of expression from high to single alignments, and included miR-21, miR-30, miR-98/Let7 family members, miR-221, miR-222, miR-622, miR-664, miR-1248 and miR-1291. [score:3]
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9
[+] score: 3
Eleven microRNAs that were differentially expressed and common in 2 of the 4 cell types were found to be miR-17, miR-21, miR-29c, miR-141, miR-142-3p, miR-215, miR-494, miRPlus-E1072, miRPlus-E1192, miR-1248, and miR-1973 (Fig. 5). [score:3]
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10
[+] score: 3
Some of the analyzed miRNAs such as miR-1286, miR-1275, miR-665, miR-602 and miR-1248 did not show any corresponding target gene (Table S4). [score:3]
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11
[+] score: 1
In all three cases, between 75% (mir-1291) and 97% (mir-1248) of the miRNA hairpin is contained within the snoRNA (using the coordinates of the UCSC Genome Browser as described in the ). [score:1]
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12
[+] score: 1
Diagnostic values were observed for let-7a-5p, let-7g-5p, miR-126-3p, miR-1248, miR-675, miR-942-5p, and miR-93-3p respectively in only two [9, 14], two [9, 10], one [9], two [10, 13], two [11, 13], two [12, 13], and four of the seven studies [9, 10, 12, 13]. [score:1]
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13
[+] score: 1
However, other microRNAs have not been described in cardiac differentiation, including hsa-mir-675-5p, hsa-mir-585-3p, hsa-mir-1248 and hsa-mir-509-5p. [score:1]
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14
[+] score: 1
Other miRNAs from this paper: hsa-mir-17, hsa-mir-28, hsa-mir-223, hsa-mir-127, hsa-mir-188, hsa-mir-194-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-30e, hsa-mir-362, hsa-mir-363, hsa-mir-367, hsa-mir-379, hsa-mir-196b, hsa-mir-450a-1, hsa-mir-431, ssc-mir-28, hsa-mir-493, hsa-mir-512-1, hsa-mir-512-2, hsa-mir-500a, hsa-mir-501, hsa-mir-502, hsa-mir-450a-2, hsa-mir-513a-1, hsa-mir-513a-2, hsa-mir-506, hsa-mir-508, hsa-mir-509-1, hsa-mir-532, hsa-mir-615, hsa-mir-660, bta-mir-127, bta-mir-30e, bta-mir-17, bta-mir-450a-2, bta-mir-532, bta-mir-363, bta-mir-660, hsa-mir-891a, hsa-mir-892a, hsa-mir-509-2, hsa-mir-450b, hsa-mir-892b, hsa-mir-708, hsa-mir-509-3, hsa-mir-1285-1, hsa-mir-1285-2, ssc-mir-17, bta-mir-155, bta-mir-188, bta-mir-194-2, bta-mir-196b, bta-mir-223, bta-mir-28, bta-mir-362, bta-mir-367, bta-mir-379, bta-mir-431, bta-mir-493, bta-mir-500, bta-mir-502a-1, bta-mir-502a-2, bta-mir-502b, bta-mir-615, bta-mir-708, bta-mir-1248-1, bta-mir-1248-2, ssc-mir-450a, bta-mir-2320, bta-mir-1388, bta-mir-194-1, bta-mir-450a-1, eca-mir-30e, eca-mir-367, eca-mir-684, eca-mir-196b, eca-mir-615, eca-mir-708, eca-mir-194-1, eca-mir-493a, eca-mir-17, eca-mir-1248, eca-mir-28, eca-mir-127, eca-mir-379, eca-mir-431, eca-mir-493b, eca-mir-155, eca-mir-194-2, eca-mir-188, eca-mir-223, eca-mir-362, eca-mir-363, eca-mir-450a, eca-mir-450b, eca-mir-450c, eca-mir-500-1, eca-mir-500-2, eca-mir-501, eca-mir-502, eca-mir-508, eca-mir-509a, eca-mir-532, eca-mir-660, ssc-mir-30e, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-127, ssc-mir-532, ssc-mir-708, ssc-mir-1285, ssc-mir-500, hsa-mir-514b, ssc-mir-363-1, ssc-mir-450c, hsa-mir-500b, ssc-mir-194b, ssc-mir-155, ssc-mir-362, bta-mir-3601, ssc-mir-615, ssc-mir-2320, bta-mir-450b, ssc-mir-194a, ssc-mir-196b-2, ssc-mir-363-2, ssc-mir-493, hsa-mir-892c, eca-mir-1388, eca-mir-514b, eca-mir-506a, eca-mir-509b, bta-mir-194b, ssc-mir-1388, ssc-mir-223, ssc-mir-660, bta-mir-194b-2, bta-mir-1949
At the same genomic position and strand, a high confident hit from miRBase is found, eca-mir-1248 is found. [score:1]
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15
[+] score: 1
miR-151a-3p, miR-181a-5p, and miR-1248 are reported to be significantly decreased with age in humans, in which all three miRNAs also show indications of associations with inflammation [31]. [score:1]
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16
[+] score: 1
Seven human miRNAs (hsa-miR-1291, hsa-miR-1246, hsa-miR-1248, hsa-miR-1274b, hsa-miR-1973, hsa-miR-4284 and hsa-miR-3656) could be accurately mapped to other ncRNAs, which included snoRNAs, snRNAs, tRNAs and rRNAs (Table 1). [score:1]
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