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12 publications mentioning bmo-bantam

Open access articles that are associated with the species Bombyx mori and mention the gene name bantam. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 28
Stage-by-stage examinations revealed further expression patterns, such as emergence at specific time-points during embryogenesis and up-regulation of miRNA groups in late embryos (miR-1 and bantam), expression associated with stage transition between instar and molt larval stages (miR-34b), expression associated with silk gland growth and spinning activity (miR-274), continuous high expression from the spinning larval to pupal and adult stages (miR-252 and miR-31a), a coordinate expression trough in day 3 pupae of both sexes (miR-10b and miR-281), up-regulation in pupal metamorphosis of both sexes (miR-29b), and down-regulation in pupal metamorphosis of both sexes (miR-275). [score:20]
Half of the 92 miRNAs passed the filtering expression threshold, 35 were also confirmed by, and 15 (including bantam and miR-1) were robustly expressed from the embryo to adult stages (Additional files 3 and 4). [score:5]
Seven "early" miRNAs were detected in freshly deposited eggs (including bantam, miR-92, and miR-184-3p), which continued to be expressed until the formation of the synkaryon (6 h Em) (Additional file 5). [score:3]
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2
[+] score: 27
Expression Patterns of sli-miR-14, sli-miR-2a and sli-bantam from S. lituraThe expression patterns of the putative miRNAs, sli-miR-14, sli-miR-2a and sli-bantam were also studied to make a further validation of this method. [score:5]
Expression Patterns of sli-miR-14, sli-miR-2a and sli-bantam from S. litura. [score:3]
Three known miRNAs, miR-14, miR-2a and bantam, play important roles in the developmental stages in D. melanogaster [23, 24], such as regulating steroid hormone signaling [25] and apoptosis [12, 26, 27]. [score:3]
The expression patterns of the putative miRNAs, sli-miR-14, sli-miR-2a and sli-bantam were also studied to make a further validation of this method. [score:3]
The homologues of miR-14, miR-2a and bantam were cloned from Spodoptera litura, a prevalent agriculture pest in China, by stem-loop RT-PCR, and their expression patterns in different developmental stages were also investigated to confirm the results of sequence analysis. [score:2]
Amplification and Identification of sli-miR-14, sli-miR-2a and sli-bantam from S. lituraTo identify the availability of stem-loop RT-PCR technology for cloning conserved miRNAs from non-mo del insects, the sequences of miR-14, miR-2a and bantam in mo del insects were searched from miRBase (Table S1). [score:1]
The putative homologue of the three miRNAs in S. litura, namely sli-miR-14, sli-miR-2a and sli-bantam were cloned by a stem-loop RT-PCR technique. [score:1]
PCR of sli-miR-14, sli-miR-2a and sli-bantam. [score:1]
Three homologues of known miRNAs, miR-14, miR-2a and bantam in mo del insects, were cloned from S. litura by stem-loop RT-PCR, and named sli-miR-14, sli- miR-2a and sli-bantam. [score:1]
Some miRNAs with evolutional variation in their 5′ region, such as bantam, cannot show accurate sequences in non-mo del insects and toned to be identified together by other methods. [score:1]
Instead, abnormal amplification appeared in qPCR of sli- bantam, there is no significant difference in Ct values in the presence or absence of cDNA added to the RT reactions (data not shown). [score:1]
To identify the availability of stem-loop RT-PCR technology for cloning conserved miRNAs from non-mo del insects, the sequences of miR-14, miR-2a and bantam in mo del insects were searched from miRBase (Table S1). [score:1]
Although putative sli-bantam could be cloned by stem-loop RT-PCR, no stable results of qPCR could be obtained here (data not shown). [score:1]
Amplification and Identification of sli-miR-14, sli-miR-2a and sli-bantam from S. litura. [score:1]
When Group 1 was used for amplification of miR-14, miR-2a and bantam, PCR products were 86 bp, 89 bp, 89 bp, respectively (Figure 1a); when Group 2 was used, PCR products were 76 bp, 79 bp and 79 bp in length, respectively (Figure 1b). [score:1]
MiR-14 and miR-2a are more conserved members of miRNAs family than bantam in mo del insects including D. melanogaster and B. mori (Table S1). [score:1]
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3
[+] score: 18
bantam miRNA exhibited constant expression levels in the body wall, silk glands and midgut, but presented significant down-regulation in the fat body (Additional file 1). [score:6]
In insects, bantam miRNA regulates cell proliferation and death by targeting the apoptosis gene hid (wrinkled) [11]. [score:4]
In all, only 10 miRNAs were universally distributed (including bmo-let-7 and bmo-bantam), while the majority were expressed exclusively or preferentially in specific tissue types (e. g., bmo-miR-275 and bmo-miR-1). [score:3]
Additionally, Northern-blot results confirmed sex differences in body wall expression of 10 miRNAs (e. g., bantam, miR-1, miR-13a and miR-2a) (Table 2, Figure 1D). [score:3]
Similar results were obtained by Northern blot analysis for bantam, miR-2a, miR-228 and miR-79 (Figure 1D). [score:1]
Based on microarray analysis, 10 female-biased miRNAs were detected in the malpighian tubules (miR-286, miR-228, miR-274, miR-1, miR-252, let-7a, miR-8, bantam, miR-200b, and miR-2a), whereas miR-276-5p and miR-305 showed male-prone accumulation in malpighian tubules. [score:1]
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4
[+] score: 15
Here, the significant up-regulation of Lig gene in infected midgut suggests that suppression of bmo-bantam may retard the growth of infected silkworm by increasing the expression of Lig gene. [score:8]
Previously, we identified the down-regulation of bmo-bantam in BmCPV infected midgut [8]. [score:4]
Moreover, Lig functions as a critical growth suppressor to control organ size via bantam (ban) microRNA and Hippo signaling in D. melanogaster [49]. [score:3]
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5
[+] score: 7
Liu et al. (2010a) were the first to report spatial expression patterns of nearly 100 miRNAs in multiple normal tissues of female and male B. mori using microarray and northern-blotting analyses, in which only 10 miRNAs were detected to express in universal tissue types of the B. mori, such as bmo-let-7 and bmo-bantam, whereas the majority were distributed exclusively or preferentially in specific tissues, such as bmo-miR-275 and bmo-miR-1. Other experimental protocols commonly used to detect miRNA expressions are polymerase chain reaction (PCR) technology, polyacryla-mide gel electrophoresis (PAGE)/northern blotting, and real-time PCR (qPCR). [score:7]
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6
[+] score: 6
bmo-let-7b, bmo-let-7c, bmo-miR-9, bmo-miR-9*, bmo-miR-100-like, bmo-miR-263a, bmo-miR-31 and bmo-bantam were expressed in larva and pupa, but were not detected in moth; of these miRNAs, bmo-miR-9 and bmo-miR-9* are also complementary miRNAs. [score:3]
We designated these sequences bmo-miR-13a*, bmo-miR-14, bmo-miR-46, bmo-miR-46*, bmo-miR-71, bmo-miR-277 and bmo-bantam. [score:1]
bmo-miR-279 and other mir-279 precursor sequences have sequence similarities of >55%; bmo-miR-277 precursors share >56% sequence similarity; bmo-miR-27 precursors share >57% sequence similarity; bmo-miR-71 precursors share >59% sequence similarity; and bmo-bantam precursors share >60% sequence similarity. [score:1]
Members of the bantam, mir-71, mir-275, mir-277, mir-279 miRNA families have a high degree of similarity in their precursor sequences. [score:1]
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7
[+] score: 3
Among the highly expressed; bantam, miR-184, miR-81, miR-100, miR-92, miR-2766, miR-279 are listed in the top (S1 Table) and these findings are in concurrence with recent reports [19]. [score:3]
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8
[+] score: 3
A number of evolutionarily conserved miRNAs (let-7a, mir-1, and bantam) were among the most abundant miRNAs, as demonstrated previously [8, 34- 37], whereas the majority of novel miRNAs (particularly the silkworm-specific components) were among the least abundant (Additional file 13, Figures 1C,1D), consistent with the correlation between evolutionary conservation of miRNAs and their expression levels [35, 38, 39]. [score:3]
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9
[+] score: 3
Among these, several conserved miRNAs such as bmo-bantam and bmo-let-7 were highly abundant suggesting that these conserved miRNAs may have important regulatory roles in Bombyx mori. [score:2]
Further analysis suggested that conserved miRNAs such as bmo-bantam, bmo-let-7, bmo-miR-31, bmo-miR-8 were in general highly abundant. [score:1]
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10
[+] score: 2
Other miRNAs from this paper: dme-bantam
Cooperative regulation of growth by Yorkie and Mad through bantam. [score:2]
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11
[+] score: 1
For instance, bmo-bantam, bmo-miR-12, bmo-miR-263a, bmo-miR-263b, bmo-miR-278, and bmo-miR-8 are both literature -based and database collections but the miRBase-collected miRNAs showed stronger signals. [score:1]
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12
[+] score: 1
Some of these miRNAs, such as bmo-bantam, bmo-miR-184, bmo-miR-2766, bmo-miR-11 and bmo-miR-100, exhibited a clear preference for binding to BmAgo2 with a high read copy of greater than 5000. [score:1]
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