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50 publications mentioning ssc-let-7a-1

Open access articles that are associated with the species Sus scrofa and mention the gene name let-7a-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 44
Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. [score:9]
0057156.g005 Figure 5 Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. [score:9]
Let-7, miR-15 and miR-133 families possessed at least two members, more interestingly, these miRNAs shared similar expression profile (up-regulated). [score:6]
The let-7 family (let-7a, let-7c) was up-regulated in this study, suggesting their transcription and maturation might also be induced directly by gonadotropin-releasing hormone. [score:5]
Prediction via software indicated that the let-7 family might target FSHβ with perfect match in seed sequence and high conservation among species (mouse and pig, data not shown), which indicated a potential role of the let-7 family in the regulation of FSH secretion. [score:4]
Microarray revealed a total of 31 differentially expressed miRNAs, most of which had been detected in pig pituitary anterior lobe with high abundance in our previous study [20], such as let-7a, let-7c, miR-361-5p, miR-320, miR-21, miR-22, miR-152 and miR-15a. [score:3]
Among all differentially expressed miRNAs, miR-133, miR-15/195 and let-7 families had two members. [score:3]
h CAAAUGC ssc-miR-19b 1.50 miR-19 GUGCAAA ssc-miR-183 1.47 miR-183 AUGGCAC ssc-miR-423-3p 1.46 miR-423/423-3p GCUCGGU ssc-miR-206 1.46 miR-1/206 GGAAUGU ssc-miR-15a 1.42 miR-15/16/195/424/497 AGCAGCA ssc-miR-22-5p 1.39 miR-22-5p/3568 GUUCUUC ssc-miR-133a-3p 1.39 miR-133 UUGGUCC ssc-miR-340 1.37 miR-340/340-5p UAUAAAG ssc-let-7a 1.37 let-7/98 GAGGUAG ssc-miR-338 1.36 miR-338/338-3p CCAGCAU ssc-miR-361-5p 1.35 miR-361/361-5p UAUCAGA 10.1371/journal. [score:1]
Previous studies showed that 100 nM GnRH caused an increase of let-7a [23]. [score:1]
h CAAAUGC ssc-miR-19b 1.50 miR-19 GUGCAAA ssc-miR-183 1.47 miR-183 AUGGCAC ssc-miR-423-3p 1.46 miR-423/423-3p GCUCGGU ssc-miR-206 1.46 miR-1/206 GGAAUGU ssc-miR-15a 1.42 miR-15/16/195/424/497 AGCAGCA ssc-miR-22-5p 1.39 miR-22-5p/3568 GUUCUUC ssc-miR-133a-3p 1.39 miR-133 UUGGUCC ssc-miR-340 1.37 miR-340/340-5p UAUAAAG ssc-let-7a 1.37 let-7/98 GAGGUAG ssc-miR-338 1.36 miR-338/338-3p CCAGCAU ssc-miR-361-5p 1.35 miR-361/361-5p UAUCAGA 10.1371/journal. [score:1]
It was reported that lin-28a played an important role in processing pri-let-7 to mature let-7 [38], [39]. [score:1]
Consequently, 27 of 31 miRNAs were mapped to 12 different pig chromosomes (Figure 3, detail in Table S2) and 3 miRNAs (ssc-let-7a, ssc-miR-340 and ssc-miR-133a-3p) had two copies in the genome. [score:1]
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2
[+] score: 29
For example, the miR-133 family (ssc-miR-133b, ssc-miR-133a-3p) as well as the let-7 family (ssc-miR-98, ssc-let-7e) was up-regulated in Bama minipigs; in the miR-1/206 family, ssc-miR-1 was up-regulated whilst ssc-miR-206 was down-regulated in Bama minipigs. [score:10]
Of these, 40 miRNAs are minimally expressed (0 < average signals ≤ 100; p ≤ 0.01), 77 miRNAs are modestly expressed 100 < average signals ≤ 1,000; p ≤ 0.01), 85 miRNAs were highly expressed (1,000 < average signals ≤ 10,000), and, in particular, 20 miRNAs were extremely highly expressed in the anterior pituitary (average signals ≥ 10,000; p ≤ 0.01), including ssc-miR-7, Y-90, ssc-miR-26a, ssc-miR-125b, Y-1, ssc-miR-125a, Y-77, ssc-let-7g, ssc-miR-29a, ssc-let-7i, ssc-let-7a, ssc-let-7f, ssc-miR-148a, ssc-miR-21, ssc-miR-335, ssc-miR-30b-5p, ssc-miR-191, ssc-miR-29c, ssc-miR-23b, and ssc-miR-23a (Fig 1C). [score:9]
It has been well demonstrated that let-7, miR-193-3p, and miR-195 were down-regulated in GH-secreting pituitary adenomas that accompanied excessive GH secretion[42, 43] and our previous study demonstrated that over -expression of ssc-let-7c in porcine pituitary cells leads to a decrease in GH secretion[44]. [score:6]
In the present study, we observed that two members of the let-7 family (let-7e and ssc-miR-98) as well as miR-193-3p and miR-195 were significantly up-regulated in Bama minipigs. [score:4]
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3
[+] score: 28
PRRSV infection of PAMs by both HP-PRRSV and N-PRRSV strains downregulated the expression of let-7f-5p and several other let-7 family members, including let-7i-5p, let-7d-5p, and let-7g-5p, resulting in greater virus infectivity. [score:6]
In order to elucidate whether PRRSV infection affects the expression of let-7f-5p, microRNA let-7 family member expression in response to PRRSV infection was checked in PAMs. [score:5]
Let-7a suppresses Kaposi’s sarcoma -associated herpesvirus (KSHV) replication through targeting of MAP4K4 signaling pathways 38. [score:4]
To understand the expression patterns of let-7 family members during PRRSV infection, we profiled host miRNAs in PAMs during HP-PRRSV and N-PRRSV infections using a deep sequencing approach. [score:3]
The let-7 family contains some members with highly conserved sequences in species ranging from worms to humans, which share several conserved mRNA targets 17 36. [score:3]
Moreover, we observed that let-7f-5p has a significant impact on PRRSV infection and this result is consistent with previous observations showing that microRNAs are small RNAs with great power; differential regulation by let-7 family miRNAs has previously been shown to participate in regulation of the immune response to many virus infections. [score:3]
Similar expression patterns were observed for several other let-7 miRNA members (Fig. 7a). [score:3]
Notably, although the members share the same “seed region”, let-7 family members are each unique and encoded on different chromosomes. [score:1]
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4
[+] score: 28
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-20a, hsa-mir-22, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-98, hsa-mir-101-1, hsa-mir-16-2, mmu-let-7g, mmu-let-7i, mmu-mir-1a-1, mmu-mir-15b, mmu-mir-101a, mmu-mir-126a, mmu-mir-130a, mmu-mir-133a-1, mmu-mir-142a, mmu-mir-181a-2, mmu-mir-194-1, hsa-mir-208a, hsa-mir-30c-2, mmu-mir-122, mmu-mir-143, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-181a-1, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-15b, hsa-mir-122, hsa-mir-130a, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-142, hsa-mir-143, hsa-mir-126, hsa-mir-194-1, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-208a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-16-1, mmu-mir-16-2, mmu-mir-18a, mmu-mir-20a, mmu-mir-22, mmu-mir-26a-1, mmu-mir-26b, mmu-mir-29c, mmu-mir-98, mmu-mir-326, rno-mir-326, rno-let-7d, rno-mir-20a, rno-mir-101b, mmu-mir-101b, hsa-mir-1-1, mmu-mir-1a-2, hsa-mir-181b-2, mmu-mir-17, mmu-mir-19a, mmu-mir-181a-1, mmu-mir-26a-2, mmu-mir-19b-1, mmu-mir-181b-1, mmu-mir-181c, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-101-2, hsa-mir-26a-2, hsa-mir-378a, mmu-mir-378a, hsa-mir-326, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, mmu-mir-181b-2, rno-let-7a-1, rno-let-7a-2, rno-let-7b, rno-let-7c-1, rno-let-7c-2, rno-let-7e, rno-let-7f-1, rno-let-7f-2, rno-let-7i, rno-mir-15b, rno-mir-16, rno-mir-17-1, rno-mir-18a, rno-mir-19b-1, rno-mir-19a, rno-mir-22, rno-mir-26a, rno-mir-26b, rno-mir-29c-1, rno-mir-30c-1, rno-mir-30c-2, rno-mir-98, rno-mir-101a, rno-mir-122, rno-mir-126a, rno-mir-130a, rno-mir-133a, rno-mir-142, rno-mir-143, rno-mir-181c, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-194-1, rno-mir-194-2, rno-mir-208a, rno-mir-181a-1, hsa-mir-423, hsa-mir-18b, hsa-mir-20b, hsa-mir-451a, mmu-mir-451a, rno-mir-451, ssc-mir-122, ssc-mir-15b, ssc-mir-181b-2, ssc-mir-19a, ssc-mir-20a, ssc-mir-26a, ssc-mir-326, ssc-mir-181c, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-18a, ssc-mir-29c, ssc-mir-30c-2, hsa-mir-484, hsa-mir-181d, hsa-mir-499a, rno-mir-1, rno-mir-133b, mmu-mir-484, mmu-mir-20b, rno-mir-20b, rno-mir-378a, rno-mir-499, hsa-mir-378d-2, mmu-mir-423, mmu-mir-499, mmu-mir-181d, mmu-mir-18b, mmu-mir-208b, hsa-mir-208b, rno-mir-17-2, rno-mir-181d, rno-mir-423, rno-mir-484, mmu-mir-1b, ssc-mir-15a, ssc-mir-16-2, ssc-mir-16-1, ssc-mir-17, ssc-mir-130a, ssc-mir-101-1, ssc-mir-101-2, ssc-mir-133a-1, ssc-mir-1, ssc-mir-181a-1, ssc-let-7e, ssc-let-7g, ssc-mir-378-1, ssc-mir-133b, ssc-mir-499, ssc-mir-143, ssc-mir-423, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-98, ssc-mir-208b, ssc-mir-142, ssc-mir-19b-1, hsa-mir-378b, ssc-mir-22, rno-mir-126b, rno-mir-208b, rno-mir-133c, hsa-mir-378c, ssc-mir-194b, ssc-mir-133a-2, ssc-mir-484, ssc-mir-30c-1, ssc-mir-126, ssc-mir-378-2, ssc-mir-451, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, mmu-mir-378b, mmu-mir-101c, hsa-mir-451b, hsa-mir-499b, ssc-let-7a-2, ssc-mir-18b, hsa-mir-378j, rno-mir-378b, mmu-mir-133c, mmu-let-7j, mmu-mir-378c, mmu-mir-378d, mmu-mir-451b, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-194a, mmu-let-7k, mmu-mir-126b, mmu-mir-142b, rno-let-7g, rno-mir-15a, ssc-mir-378b, rno-mir-29c-2, rno-mir-1b, ssc-mir-26b
Thus, miRNA families (e. g., miR-1 and miR-122) that are specifically or highly expressed in any one of the 3 tissues, or miRNAs that are expressed ubiquitously (e. g., let-7 and miR-26) in all 3 tissues, show a far greater frequency than other miRNAs. [score:5]
Interestingly, the expression abundance varies among the let-7 family members (Tables 1 and 2); let-7a and let-7j, each have 80 reads; similarly, let-7b, let-7c and let-7e have almost the same number of reads (63–64); let-7d, let-7f and let-7j have 18 to 32 reads; and let-7h, let-7i and let-7k have a lower number of reads (5–9) (Tables 1 and 2). [score:3]
For instance, let-7 is represented by 445 reads and miR-26 by 177 reads (Tables 1 and 2), and these two miRNAs are ubiquitously expressed in the heart, liver and thymus (Figure 3A and 3B). [score:3]
Hence the let-7 miRNA family is represented by 11 members, and this study provides the evidence for the expression of all 11 let-7 family members in pig. [score:3]
Here, we found evidence for the expression of all 10 let-7 members in pig. [score:3]
Additionally, many other miRNAs, such as let-7, miR-98, miR-16, miR22, miR-26b, miR-29c, miR-30c and miR126, were also expressed abundantly in thymus (Figure 3). [score:3]
Similarly, let-7, miR-98, miR-16 and miR-130a are abundantly expressed in 13 of the 14 tissues (except in pancreas) (Figure 3A). [score:3]
let-7, miR-98, miR-130a and miR-16 showed uniform levels of expression in 13 different tissues but were hardly detected in pancreas (Figure 3A). [score:3]
The miR-98 sequence differs from that of the let-7 family by one nt at position 11 from the 5' end, thus miR-98 is also a member of the let-7 family. [score:1]
The let-7 family has 10 members in diverse animal species (miRBase). [score:1]
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5
[+] score: 24
In order to verification the differential expressed miRNAs, four down-regulated (let-7a, miR-16, miR-20a, miR-20b-5p) and 4 up-regulated miRNAs (miR-143, miR-192, miR-133b, miR-223) were randomly selected and analyzed using Real-time PCR method (Figure 6, A). [score:9]
Let-7 miRNAs were differentially expressed between Tongcheng and Large White pigs, indicating the gene expression regulation at posttranscriptional level in liver is different between lean and fatty pigs at the same body weight. [score:6]
Let-7a was the most highly expressed let-7 family member, it has been confirmed as a negative regulation signal transducer and activator of transcription 3 (STAT3), which is associated to hepatocellular proliferation and hepatocarcinogenesis [45]. [score:4]
These results indicated that let-7 family members play important roles in liver development [46]. [score:2]
The human let-7/98 family contains 9 members, i. e., hsa-let-7a/b/c/d/e/f/g/i and hsa-miR-98. [score:1]
The rat let-7/98 family has 8 members (let-7g is not included). [score:1]
We found that the porcine let-7/98 family has 12 members including the 9 human homologous and ssc-let-7h/j/k miRNAs (Table S16). [score:1]
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6
[+] score: 21
The unified set of top 10 unique miRNAs over the two pig breeds correspond to 15 unique miRNAs, 11 of which (ssc-let-7a-1/2-5p, ssc-let-7c-5p, ssc-let-7e-5p, ssc-miR-10a-5p, ssc-miR-10b-5p, ssc-miR-127-3p, ssc-miR-148a-3p, ssc-miR-199a-1/2-5p, ssc-miR-21-5p, ssc-miR-26a-5p, ssc-miR-125b-1-5p) had been frequently reported highly expressed in skeletal muscle during porcine prenatal and postnatal developmental stages. [score:4]
Study of Mai et al. revealed that ssc-let-7a-1/2-5p, ssc-miR-10a-5p, ssc-miR-127-3p, ssc-miR-148a-3p, ssc-miR-199a-1/2-5p, ssc-miR-26a-5p were the most highly expressed unique miRNAs over five porcine muscle developmental stages from 90 dpc to 7 y after birth [27]. [score:4]
Let-7a, let-7c and let-7i (members of let-7 family) are regulators in development, and in cellular basal metabolism [30]. [score:3]
Some myogenesis related miRNAs (miR-133, miR-1, miR-206 and miR-148a) are highly abundant in MS pigs, while other miRNAs (let-7 family, miR-214, miR-181) highly expressed in LW. [score:3]
Interestingly, Among them, miR-133, miR-1, miR-206 and miR-148a were highly abundant in MS pigs, while let-7 family, miR-214 and miR-181 were highly expressed in LW. [score:3]
For example, Qin et al. had reported that ssc-let-7a, ssc-miR-10a, ssc-miR-10b, ssc-miR-127, ssc-miR-148a, ssc-miR-21, ssc-miR-26a were the most abundant miRNAs during porcine skeletal muscle developmental stages from 35 days post coitum to postnatal day 180 [25]. [score:2]
Hou et al. shows that ssc-let-7a, ssc-let-7c and ssc-miR-26a were the common most abundant miRNAs in longissimus dorsi muscle of three pig breeds (Landrace, Tongcheng, and Wuzhishan) at postnatal day 240 [26]. [score:1]
The let-7 family of microRNAs. [score:1]
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7
[+] score: 21
The expression of C9orf19 was potentially regulated by miR-1, miR-133b, miR-206 and let-7a. [score:4]
There were 22, 21, 10, 9 and 9 target mRNAs for miR-765, miR-302b, miR-1, let-7a and miR-206, respectively, suggesting that these miRNAs significantly contribute to prenatal skeletal muscle development in Tongcheng pigs. [score:4]
Eight miRNAs (miR-765, miR-302b, miR-1, let-7a, miR-206, miR-500, miR-546 and miR-705) targeted 86.1% of the mRNAs in Tongcheng pigs. [score:3]
We also found that CCNJ had potentially common target loci: let-7a, let-7b, let-7d, miR-198, miR-206, miR-207, miR-325, miR-370 and miR-697. [score:3]
, 32 miRNAs, including let-7b, let-7d and let-7i from the let-7 family, were differentially expressed during myogenesis. [score:3]
Similarly, the dystrophin (DMD) gene, involved in Duchenne muscular dystrophy 63, was potentially regulated by let-7a, let-7b, let-7d, miR-207 and miR-325. [score:2]
FKBP1B and HMGCS1, which encode rate-limiting enzymes of the cholesterol synthesis pathway 61, were potentially regulated by let-7a, miR-1 and miR-206. [score:2]
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8
[+] score: 21
It has been established that miRNAs can target specific genes [23]; in the present study, let-7c, let-7 g, miR-18a, miR-196b, and miR-9 targeted MAP3K7, and miR-196b and miR-19b targeted dipeptidyl-peptidase 8 (DPP8), suggesting that cellular miRNAs play a key role in regulating gene expression in response to PPV infection. [score:10]
Among them, let-7 g, miR-17-5p, miR-17-3p, miR-20a, miR-181a, miR-16, miR-146b, miR-10b, and miR-155-5p were upregulated; let-7c, miR-122, miR-18a, miR-19a, miR-19b, miR-196b, miR-21, and miR-9 were downregulated. [score:7]
Let-7 g, let-7c, miR-19b, and miR-16 are involved in immune-related programs and may act through the target gene IL10. [score:3]
Many immune-related miRNAs have been identified in innate and adaptive immune systems, including the miR-17—92 cluster, miR-221, miR-10, miR-196b, miR-126, miR-155, miR-150; miR-181a, miR-326, miR-142-3p, miR-424, miR-21, miR-106a, miR-223, miR-146; the let-7 family, miR-9, and miR-34 [6]. [score:1]
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9
[+] score: 20
Other miRNAs from this paper: ssc-mir-122, ssc-mir-125b-2, ssc-mir-181b-2, ssc-mir-20a, ssc-mir-23a, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-21, ssc-mir-29c, ssc-mir-30c-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-20a, bta-mir-21, bta-mir-26b, bta-mir-30d, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-30b, bta-mir-107, bta-mir-10a, bta-mir-127, bta-mir-142, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-138-2, bta-mir-17, bta-mir-181c, bta-mir-192, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-214, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-99b, ssc-mir-99b, ssc-mir-17, ssc-mir-30b, ssc-mir-199b, bta-mir-1-2, bta-mir-1-1, bta-mir-129-1, bta-mir-129-2, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-135b, bta-mir-138-1, bta-mir-143, bta-mir-144, bta-mir-146b, bta-mir-146a, bta-mir-181d, bta-mir-190a, bta-mir-199a-2, bta-mir-202, bta-mir-206, bta-mir-211, bta-mir-212, bta-mir-223, bta-mir-26a-1, bta-mir-29d, bta-mir-30f, bta-mir-338, bta-mir-33a, bta-mir-33b, bta-mir-375, bta-mir-429, bta-mir-451, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-133a-1, ssc-mir-1, ssc-mir-146b, ssc-mir-181a-1, ssc-mir-30a, bta-mir-199c, ssc-mir-206, ssc-let-7e, ssc-let-7g, ssc-mir-133b, ssc-mir-29a, ssc-mir-30d, ssc-mir-30e, ssc-mir-199a-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-10b, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-99a, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-192, ssc-mir-142, ssc-mir-127, ssc-mir-202, ssc-mir-129a, ssc-mir-455, ssc-mir-125b-1, ssc-mir-338, ssc-mir-133a-2, ssc-mir-146a, bta-mir-26c, ssc-mir-30c-1, ssc-mir-126, ssc-mir-199a-1, ssc-mir-451, ssc-let-7a-2, ssc-mir-129b, ssc-mir-429, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-132, ssc-mir-138, ssc-mir-144, ssc-mir-190a, ssc-mir-212, bta-mir-133c, ssc-mir-26b, ssc-mir-200b, ssc-mir-223, ssc-mir-375, ssc-mir-33b
They detected let-7a-3p as a regulator of zonadhesin-like and growth hormone 2 gene, miR-142-5p as a regulator of heparin -binding growth factor 1, and miR-144 as a regulator of the growth factor receptor-bound protein 2. They also found that miR-430 regulates the transforming growth factor-beta -induced protein ig-h3, miR-451 blocks the anti-dorsalizing morphogenic protein, and miR-1594 activates both titin-cap (telethonin)-like mRNA and growth hormone receptor isoform 2. All of these miRNAs and targets are associated with growth and developmental processes. [score:8]
Some muscle miRNAs have well-defined target genes, as miR-206 regulates IGF-1; miR-1, miR-122, and miR-462 control IGF-2a; the let-7 family regulates MSTN; miR-103 and miR-107 modulate GHR and FSHR; and miR-138 and miR-211 control LHR. [score:5]
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. [score:3]
The let-7 family is known to regulate multiple genes related to the cell cycle and proliferation (Yang et al., 2008), and let-7i was shown to influence innate immunity (Chen et al., 2007). [score:2]
The first miRNAs, lin-4 and let-7, were both discovered in Caenorhabditis elegans (Lee et al., 1993; Wightman et al., 1993; Reinhart et al., 2000) and have subsequently been found to correspond to a novel and extensive class of small non-coding RNAs (Lagos-Quintana et al., 2001; Lau et al., 2001; Lee and Ambros, 2001). [score:1]
A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. [score:1]
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-18a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-21, hsa-mir-23a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-96, hsa-mir-98, hsa-mir-99a, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-99a, mmu-mir-127, mmu-mir-128-1, mmu-mir-136, mmu-mir-142a, mmu-mir-145a, mmu-mir-10b, mmu-mir-182, mmu-mir-183, mmu-mir-187, mmu-mir-193a, mmu-mir-195a, mmu-mir-200b, mmu-mir-206, mmu-mir-143, hsa-mir-139, hsa-mir-10b, hsa-mir-182, hsa-mir-183, hsa-mir-187, hsa-mir-210, hsa-mir-216a, hsa-mir-217, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-224, hsa-mir-200b, mmu-mir-302a, mmu-let-7d, mmu-mir-106a, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-128-1, hsa-mir-142, hsa-mir-143, hsa-mir-145, hsa-mir-127, hsa-mir-136, hsa-mir-193a, hsa-mir-195, hsa-mir-206, mmu-mir-19b-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-18a, mmu-mir-21a, mmu-mir-23a, mmu-mir-31, mmu-mir-92a-2, mmu-mir-96, mmu-mir-98, hsa-mir-200c, mmu-mir-17, mmu-mir-139, mmu-mir-200c, mmu-mir-210, mmu-mir-216a, mmu-mir-219a-1, mmu-mir-221, mmu-mir-222, mmu-mir-224, mmu-mir-19b-1, mmu-mir-92a-1, mmu-mir-128-2, hsa-mir-128-2, mmu-mir-217, hsa-mir-200a, hsa-mir-302a, hsa-mir-219a-2, mmu-mir-219a-2, hsa-mir-363, mmu-mir-363, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-371a, hsa-mir-18b, hsa-mir-20b, hsa-mir-452, mmu-mir-452, ssc-mir-106a, ssc-mir-145, ssc-mir-216-1, ssc-mir-217-1, ssc-mir-224, ssc-mir-23a, ssc-mir-183, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-128-1, ssc-mir-136, ssc-mir-139, ssc-mir-18a, ssc-mir-21, hsa-mir-146b, hsa-mir-493, hsa-mir-495, hsa-mir-497, hsa-mir-505, mmu-mir-20b, hsa-mir-92b, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, hsa-mir-671, mmu-mir-216b, mmu-mir-671, mmu-mir-497a, mmu-mir-495, mmu-mir-146b, mmu-mir-708, mmu-mir-505, mmu-mir-18b, mmu-mir-493, mmu-mir-92b, hsa-mir-708, hsa-mir-216b, hsa-mir-935, hsa-mir-302e, hsa-mir-302f, ssc-mir-17, ssc-mir-210, ssc-mir-221, mmu-mir-1839, ssc-mir-146b, ssc-mir-206, ssc-let-7e, ssc-let-7g, ssc-mir-128-2, ssc-mir-143, ssc-mir-10b, ssc-mir-23b, ssc-mir-193a, ssc-mir-99a, ssc-mir-98, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-142, ssc-mir-497, ssc-mir-195, ssc-mir-127, ssc-mir-222, ssc-mir-708, ssc-mir-935, ssc-mir-19b-2, ssc-mir-19b-1, ssc-mir-1839, ssc-mir-505, ssc-mir-363-1, hsa-mir-219b, hsa-mir-371b, ssc-let-7a-2, ssc-mir-18b, ssc-mir-187, ssc-mir-218b, ssc-mir-219a, mmu-mir-195b, mmu-mir-145b, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-31, ssc-mir-182, ssc-mir-216-2, ssc-mir-217-2, ssc-mir-363-2, ssc-mir-452, ssc-mir-493, ssc-mir-671, mmu-let-7k, ssc-mir-7138, mmu-mir-219b, mmu-mir-216c, mmu-mir-142b, mmu-mir-497b, mmu-mir-935, ssc-mir-9843, ssc-mir-371, ssc-mir-219b, ssc-mir-96, ssc-mir-200b
Let-7a was highly expressed in both types of piPSCs but had a low expression level in pEFs. [score:4]
However, the mpiPSCs did not show down-regulation of most let-7 family members, including let-7c, let-7d, let-7e, let-7f, let-7g and let-7i. [score:4]
Inhibition of the let-7 family by Lin 28a, a very important pluripotent factor, improves the efficiency of somatic cell reprogramming [39]. [score:3]
The expression of the let-7 family was investigated because inhibition of the let-7 family was previously suggested to promote the reprogramming of somatic cells into piPSCs. [score:3]
MiR-98 belongs to the let-7 family and also targets MYC [38]. [score:3]
We found that most members of the let-7 family (except let-7a) were down-regulated in hpiPSCs compared with pEFs (Fig 5B). [score:3]
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A previous study reported that overexpression of let-7 resulted in insulin resistance and impaired glucose tolerance, implying that the lin28/let-7 pathway regulates glucose metabolism [34]. [score:4]
In another study, it was reported that miRNA let-7 expression is regulated by glucose [35]. [score:4]
MiRNAs including let-7, miR-1224 and miR-2288 that correlated with glucose levels were also correlated with target mRNAs belonging to functions of carbohydrate metabolism, including ACACA, ATXN2, FASN, ESR1, FUT8, SCD, CTH, GPR39, ITGB3, MLXIPL, BCL2, RGL1, MTAP, PGM2 and SLC16A6. [score:3]
We found that the expression of let-7 family mRNA negatively correlated with GLU levels, positively correlated with erythrocytes and negatively correlated with LYM. [score:3]
The other five miRNA transcripts were identified as locally regulated SNPs associated within the same chromosome of the probe-set/gene, including miR-30e, miR-19a, let-7 g, miR-4507 and miR-27d. [score:2]
As discussed above, the let-7 family plays a significant role in glucose metabolism; this study shows the complex levels of regulation. [score:2]
Five other miR (miR-30e, miR-19a, miR-4507, miR-27d and let-7 g) were associated with SNPs on the same chromosome. [score:1]
A number of studies have also demonstrated that let-7 miRNAs levels drop after infection, which in turn increases IL-10 mRNA [36]. [score:1]
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Let-7a inhibits cell growth and tumor formation [33], while let-7f inhibits the invasive and metastatic potency of gastric cancer lines by downregulation of MYH9 (a metastasis -associated gene) [34]. [score:8]
Ssc-let-7a was highly expressed in all the different organs, which suggested that this type of miRNA might participate wi dely in regulations throughout the whole body. [score:4]
The let-7 family is a conserved family of miRNAs, the members of which behave as tumor suppressors [32]. [score:3]
Moreover, other miRNAs were also expressed abundantly in skeletal muscles, including ssc-let-7a, ssc-let-7c, ssc-let-7f, ssc-miR-143-3p, ssc-miR-10b, ssc-miR-148a, ssc-miR-127, ssc-miR-30d, ssc-miR-30a-5p, and ssc-miR-181a. [score:3]
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ADORA1 (Adenosine receptor A1), which showed downregulated expression in the DSP-TP, was the target of three miRNAs, let-7a, let-7c, and let-7e. [score:8]
Five miRNAs, three highly expressed in the LL-YY (miR-let-7a, miR-let-7c, and miR-let-7e) and two highly expressed in the DSP-TP (miR-339 and miR-362), were identified as targeting ADORA1 gene. [score:7]
Some DE miRNAs, such as miR-29b, miR-122, miR-145-5p, let-7c, miR-4332, miR-182, miR-92b-3p, miR-29c,let-7a, and let-7e, and some DEGs such as CAV2, MYOZ2, FRZB, FASN, SCD, ESR1, and ADORA1, may be factors in the regulation of muscle growth and lipid deposition. [score:2]
Based on the RPKM values, the top four of the DE miRNAs were miR-let-7a, miR-99a, miR-374a-5p, and miR-148b-3p, whose sum of RPKM varied from approximately 2,035 to 9,865. [score:1]
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-29a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-208a, hsa-mir-148a, hsa-mir-10a, hsa-mir-181a-2, hsa-mir-181c, hsa-mir-199a-2, hsa-mir-181a-1, hsa-mir-214, hsa-mir-221, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-23b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-128-1, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-143, hsa-mir-125b-2, hsa-mir-126, hsa-mir-127, hsa-mir-206, hsa-mir-1-1, hsa-mir-128-2, hsa-mir-29c, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-148b, hsa-mir-133b, hsa-mir-424, ssc-mir-125b-2, ssc-mir-148a, ssc-mir-23a, ssc-mir-24-1, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-mir-27a, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-128-1, ssc-mir-29c, hsa-mir-486-1, hsa-mir-499a, hsa-mir-503, hsa-mir-411, hsa-mir-378d-2, hsa-mir-208b, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-17, ssc-mir-221, ssc-mir-133a-1, ssc-mir-1, ssc-mir-503, ssc-mir-181a-1, ssc-mir-206, ssc-let-7e, ssc-let-7g, ssc-mir-378-1, ssc-mir-133b, ssc-mir-29a, ssc-mir-199a-2, ssc-mir-128-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-486-1, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-23b, ssc-mir-148b, ssc-mir-208b, ssc-mir-424, ssc-mir-127, ssc-mir-125b-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-411, ssc-mir-133a-2, ssc-mir-126, ssc-mir-199a-1, ssc-mir-378-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-499b, ssc-let-7a-2, ssc-mir-486-2, hsa-mir-378j, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, hsa-mir-486-2, ssc-mir-378b
To identify whether highly expressed miRNAs play key roles in skeletal muscle development, the target genes of 25 miRNAs with the most abundance (not including let-7 family for their ubiquitous expression) in ten libraries (Table 1) were predicted and total 2325 target genes were found. [score:10]
0072418.g003 Figure 3 A. 31 significantly enriched KEGG pathways were achieved using the target genes of the most abundant 25 miRNAs (not including let-7 family) in ten libraries; B. comparison of KEGG pathways significantly enriched during skeletal muscle development at 35 to 77dpc (LR 1-4), 77dpc to 28 dpn (LR 4-7) and 28 to 180 dpn (LR 7-10). [score:4]
KEGG Orthology analysis of the most abundant 25 common miRNAs (excluding let-7 family) from 10 libraries indicated that their involvement in the regulation of canonical myogenesis-related pathways and diseases (Figure 5). [score:4]
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For example, ssc-miR-19a targets TNFα, IL-12α, and IL-20; ssc-miR-19b targets TNFα and IL-20; ssc-miR-7 targets IL-1β, IL-2, and TGF-β1; ssc-let-7a targets IFN- delta-1; ssc-miR-30a-5p targets IL-12α and IL-12β; and ssc-miR-24-3p targets IL-1α. [score:13]
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We used no-error mapping to differentiate the single base difference in mapping mammalian let-7 -family miRNAs, and identified eight let-7 -family miRNAs besides let-7j, which were expressed throughout development in all ten libraries (Table S14) and exhibited high levels of sequence conservation in mammals (Figure S3). [score:4]
The let-7 -family miRNAs, one of the key miRNA regulators in development, are present in abundance across various species including mammals, flies, worms and plants [38]. [score:3]
The alignments of each sequenced let-7 miRNAs with the corresponding mammalian let-7 -family of miRNAs: (B) PN(a)1-1-5P (as same sequence as PN(a)1-2-5p) aligned to homologous let-7a; (C) PN(a)2-5p aligned to homologous let-7b; (D) ssc-let-7c-5p (known porcine miRNA) aligned to the corresponding miRBase ssc-let-7c; (E) PN(a)3-5p aligned to homologous let-7d; (F) PN(a)4-5p aligned to homologous let-7e; (G) ssc-let-7f-5p (known porcine miRNA) aligned to the corresponding miRBase ssc-let-7f; (H) PN(a)6-5p aligned to homologous let-7g; (I) ssc-let-7i-5p (known porcine miRNA) aligned to the corresponding miRBase ssc-let-7i. [score:1]
At present, there are nine members of the let-7 -family in mammals: let-7a to -7g, let-7i and let-7j (only identified for dog). [score:1]
The high-count miRNAs include the muscle-specific miR-1a-3p, liver-specific miR-122-5p, and the ubiquitous let-7 -family miRNAs. [score:1]
Figure S3Sequence alignments of the sequenced let-7 -family miRNAs and the homologous mammalian let-7 family miRNAs. [score:1]
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let-7 down-regulation relieved suppression of the major macrophage cytokines IL-6 and IL-10 [14, 15]. [score:6]
Schulte et al. [14] demonstrated that the let-7 family of miRNAs was down-regulated in macrophages infected with Salmonella Typhimurium or challenged with purified LPS. [score:4]
In addition to miR-146 and miR-155, the let-7, miR-15, miR-128 and miR-29a also have roles in Salmonella infection. [score:1]
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The expression pattern of the Let-7 gene family is another conflicting result since the expression of the Let-7 gene family was undetected in this study, while previously it accounted for 7.7% of the total miRNA expression in pig fat [30]. [score:7]
On the other hand, some miRNAs were also reported as inhibitors of adipocyte differentiation, such as miR-27b and let-7 [12, 13]. [score:3]
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Whereas, overexpression of let-7 [9], miR-27b [8] and miR-448 [7] inhibits 3T3-L1 adipogenesis by targeting HMGA2 (high-mobility group AT-hook 2), PPARγ and klf5 (kruppel-like factor 5), respectively. [score:7]
1742-4658.2009.06967. x 19348006 9. Sun T. Fu M. Bookout A. L. Kliewer S. A. Mangelsdorf D. J. Microrna let-7 regulates 3t3-l1 adipogenesis Mol. [score:2]
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According to the high-throughput sequencing data, the let-7 family (let-7a, let-7f, let-7 g), the miR-10 family (miR-10b, miR-10a-3p, miR-10a-5p), miR-21, miR-143-3p, miR-30a-5p, miR-16 and miR-192 had the highest expression levels among the 10 expression profiles (Additional file  1: Figure S3), which suggests that these miRNAs are highly conserved among different organs in the same species. [score:5]
For example, ssc-let-7a, ssc-let-7c, ssc-let-7f, ssc-let-7 g and ssc-let-7i from the let-7 family and ssc-miR-21 maintained high transcription levels in all samples, which is consistent with previous findings [23] (Additional file  1: Figure S3). [score:1]
The first miRNA to be discovered was let-7, which was identified in 2001 in C. elegans. [score:1]
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The small RNA sequencing data showed the upregulation of the miR-302b/367 and miR-106a/363 clusters, and downregulation of let-7 family members and the miR-17/92 cluster. [score:7]
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In accordance with the most stable miRNAs described in the literature [20]– [22], [24], [26]– [28], ten candidate miRNAs (Ssc-let-7a, Ssc-miR-103, Ssc-miR-17-3p, Hsa-miR-25, Hsa-miR-93, Ssc-miR-106a, Ssc-miR-191, Ssc-miR-16, Ssc-miR-26a and Ssc-miR-17-5p, Table 1) were selected to study their expression stability in different porcine tissues and breeds. [score:3]
curve correlation mean * Ssc-let-7a 125 1/2000 95.52% (2.11%) 0.9991 (0.0005) Ssc-miR-103 250 1/2000 96.27% (5.15%) 0.9981 (0.0013) Ssc-miR-17-3p 250 1/200 99.96% (7.25%) 0.9971 (0.0026) Hsa-miR-25 250 1/2000 97.14% (3.76%) 0.9989 (0.0004) Hsa-miR-93 200 1/2000 98.10% (3.12%) 0.9973 (0.0012) Ssc-miR-106a 250 1/2000 99.73% (11.05%) 0.9978 (0.0015) Ssc-miR-191 250 1/2000 97.45% (3.91%) 0.9978 (0.0009) Ssc-miR-16 250 1/2000 98.31% (4.98%) 0.9990 (0. 0004) Ssc-miR-26a 250 1/2000 93.35% (0.62%) 0.9991 (0.0005) Ssc-miR-17-5p 250 1/2000 98.52% (5.00%) 0.9980 (0.0009) * The numbers in brackets denote the standard error for the mean values. [score:1]
In ovary the best options are Let-7a, miR-25 and miR-106a, while in uterus we recommend to use miR-103, Let-7a and miR-93. [score:1]
If the study is focused in skeletal muscle, we encourage using Let-7a, miR-17-5p and miR-25, but if we are working with liver, the most stable miRNAs to be used as reference miRNAs are miR-26a, miR-103 and Let-7a. [score:1]
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Ssc-let-7a, ssc-let-7f, ssc-let-7c were highly expressed throughout prenatal and postnatal development in our study. [score:4]
Aside from ssc-miR-206 and ssc-miR-1, ssc-miR-378 was the most abundant at E90, followed by ssc-miR-143-3p, ssc-let-7a, ssc-let-7f, ssc-let-7c, ssc-miR-30d, ssc-miR-30a-5p, ssc-miR-10b, ssc-miR-127, ssc-miR-148a, ssc-miR-126, ssc-miR-7i, and ssc-miR-21. [score:1]
The ssc-let-7 family is a conserved family of miRNAs. [score:1]
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[+] score: 6
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-99a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181a-1, hsa-mir-215, hsa-mir-223, hsa-mir-224, hsa-mir-200b, hsa-mir-15b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-141, hsa-mir-143, hsa-mir-152, hsa-mir-125b-2, hsa-mir-126, hsa-mir-146a, hsa-mir-184, hsa-mir-200c, hsa-mir-155, hsa-mir-29c, hsa-mir-200a, hsa-mir-99b, hsa-mir-296, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-342, hsa-mir-148b, hsa-mir-451a, ssc-mir-125b-2, ssc-mir-148a, ssc-mir-15b, ssc-mir-184, ssc-mir-224, ssc-mir-23a, ssc-mir-24-1, ssc-mir-26a, ssc-mir-29b-1, ssc-let-7f-1, ssc-mir-103-1, ssc-mir-21, ssc-mir-29c, hsa-mir-486-1, hsa-mir-499a, hsa-mir-671, hsa-mir-378d-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-16b, bta-mir-21, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-31, bta-mir-15b, bta-mir-215, bta-mir-30e, bta-mir-148b, bta-mir-192, bta-mir-200a, bta-mir-200c, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-10b, bta-mir-24-2, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-mir-342, bta-let-7f-1, bta-let-7a-2, bta-let-7a-3, bta-mir-103-2, bta-mir-125b-2, bta-mir-15a, bta-mir-99b, hsa-mir-664a, ssc-mir-99b, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-15a, ssc-mir-16-2, ssc-mir-16-1, bta-mir-141, bta-mir-143, bta-mir-146a, bta-mir-152, bta-mir-155, bta-mir-16a, bta-mir-184, bta-mir-24-1, bta-mir-223, bta-mir-224, bta-mir-26a-1, bta-mir-296, bta-mir-29d, bta-mir-378-1, bta-mir-451, bta-mir-486, bta-mir-671, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, ssc-mir-181a-1, ssc-mir-215, ssc-mir-30a, bta-mir-2318, bta-mir-2339, bta-mir-2430, bta-mir-664a, bta-mir-378-2, ssc-mir-378-1, ssc-mir-29a, ssc-mir-30e, ssc-mir-499, ssc-mir-143, ssc-mir-10b, ssc-mir-486-1, ssc-mir-152, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-99a, ssc-mir-148b, ssc-mir-664, ssc-mir-192, ssc-mir-342, ssc-mir-125b-1, oar-mir-21, oar-mir-29a, oar-mir-125b, oar-mir-181a-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-296, ssc-mir-155, ssc-mir-146a, bta-mir-148c, ssc-mir-126, ssc-mir-378-2, ssc-mir-451, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-451b, hsa-mir-499b, ssc-let-7a-2, ssc-mir-486-2, hsa-mir-664b, hsa-mir-378j, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-31, ssc-mir-671, bta-mir-378b, bta-mir-378c, hsa-mir-486-2, oar-let-7a, oar-let-7f, oar-mir-103, oar-mir-10b, oar-mir-143, oar-mir-148a, oar-mir-152, oar-mir-16b, oar-mir-181a-2, oar-mir-200a, oar-mir-200b, oar-mir-200c, oar-mir-23a, oar-mir-26a, oar-mir-29b-1, oar-mir-30a, oar-mir-99a, bta-mir-664b, chi-let-7a, chi-let-7f, chi-mir-103, chi-mir-10b, chi-mir-125b, chi-mir-126, chi-mir-141, chi-mir-143, chi-mir-146a, chi-mir-148a, chi-mir-148b, chi-mir-155, chi-mir-15a, chi-mir-15b, chi-mir-16a, chi-mir-16b, chi-mir-184, chi-mir-192, chi-mir-200a, chi-mir-200b, chi-mir-200c, chi-mir-215, chi-mir-21, chi-mir-223, chi-mir-224, chi-mir-2318, chi-mir-23a, chi-mir-24, chi-mir-26a, chi-mir-27b, chi-mir-296, chi-mir-29a, chi-mir-29b, chi-mir-29c, chi-mir-30a, chi-mir-30e, chi-mir-342, chi-mir-378, chi-mir-451, chi-mir-499, chi-mir-671, chi-mir-99a, chi-mir-99b, bta-mir-378d, ssc-mir-378b, oar-mir-29b-2, ssc-mir-141, ssc-mir-200b, ssc-mir-223, bta-mir-148d
Similarly, Jin et al. (2014a) demonstrated a differential expression of nine miRNAs (bta-miR-184, miR-24-3p, miR-148, miR-486, and let-7a-5p, miR-2339, miR-499, miR-23a, and miR-99b) upon challenge of MACT-cells (bovine mammary epithelia cell line) with heat inactivated E. coli and S. aureus bacteria. [score:3]
Additionally, a number of miRNAs including miR-148a, miR-26a, miR-21-5p, miR-27b, miR-143, bta-miR-30a-5p, let-7a-5p, let-7f, miR-10b, and miR-99a-5p are highly expressed in bovine mammary gland/mammary epithelial cells (Li et al., 2012a, 2014a; Jin et al., 2014a; Le Guillou et al., 2014) suggesting roles in the lactation process and mammary gland functions. [score:3]
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[+] score: 6
This is consistent with previous reports that ssc-let-7 is highly expressed in various cell types and species [22]. [score:3]
Among the 15 most abundant miRNAs, the most strongly expressed miRNA in both libraries was ssc-let-7 (f, a, d, e, g, c), which represented 74.98% and 74.39% of the total miRNA reads in two samples, respectively. [score:3]
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26
[+] score: 5
The most abundant identified miRNA was miR-21, followed by two members of the let-7 family (ssc-let-7f, ssc-let-7c) known to be the most abundantly expressed in higher eukaryotes. [score:3]
let-7, miR-21 and others) [40], [41]. [score:1]
For example, only six members of Let-7 miRNA family are currently annotated for the pig (miRBase, release 16), however by comparative analysis of porcine sequence tags with human miRNAs all the eleven members of this miRNA family could be identified by the present study. [score:1]
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[+] score: 5
Ssc-let-7f is an ubiquitous miRNA from let-7 family, related to many pathways involved in wide range of physiological functions (cellular, metabolic and environmental information processes) and also to many diseases, like renal cell carcinoma [81], [82]. [score:3]
Hsa-let-7a, Hsa-miR-25 and Hsa-miR-103 were used as reference miRNAs [50], [51]. [score:1]
Hsa-let-7a, Hsa-miR-25 and Hsa-miR-93 were used as reference miRNAs based on literature [50], [51]. [score:1]
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[+] score: 5
To gain insight into the general functions of miRNAs in the pituitary, the 10 most abundant miRNAs, except for the let-7 family that is well known to be ubiquitously expressed, were selected for predicting target genes and classified according to KEGG functional annotation using DAVID bioinformatics resources [33]. [score:5]
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[+] score: 5
The porcine ZNF265 3′ UTR contains a putative target site for miR-139-5p, whereas the porcine RGS16 3′ UTR was predicted to have a single site targeted by the let-7 miRNA family members let-7b-5p, let-7c and let-7e (Table  2). [score:5]
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[+] score: 5
Members of the let-7 family are broadly considered to be tumour suppressors, downregulating several oncogenes including KRAS [18]. [score:5]
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31
[+] score: 4
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-27a, hsa-mir-30a, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-107, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-30a, mmu-mir-30b, mmu-mir-125b-2, mmu-mir-9-2, mmu-mir-150, mmu-mir-24-1, mmu-mir-204, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-30e, hsa-mir-204, hsa-mir-210, hsa-mir-221, hsa-mir-222, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-150, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-21a, mmu-mir-24-2, mmu-mir-27a, mmu-mir-103-1, mmu-mir-103-2, mmu-mir-326, mmu-mir-107, mmu-mir-17, mmu-mir-210, mmu-mir-221, mmu-mir-222, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-125b-1, hsa-mir-30c-1, hsa-mir-30e, hsa-mir-378a, mmu-mir-378a, hsa-mir-326, ssc-mir-125b-2, ssc-mir-24-1, ssc-mir-326, ssc-mir-27a, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-204, ssc-mir-21, ssc-mir-30c-2, ssc-mir-9-1, ssc-mir-9-2, hsa-mir-378d-2, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-15a, ssc-mir-17, ssc-mir-30b, ssc-mir-210, ssc-mir-221, ssc-mir-30a, ssc-let-7e, ssc-let-7g, ssc-mir-378-1, ssc-mir-30d, ssc-mir-30e, ssc-mir-103-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-222, ssc-mir-125b-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-30c-1, ssc-mir-378-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, mmu-mir-378b, ssc-let-7a-2, hsa-mir-378j, mmu-mir-21b, mmu-let-7j, mmu-mir-378c, mmu-mir-21c, mmu-mir-378d, mmu-mir-30f, ssc-let-7d, ssc-let-7f-2, ssc-mir-9-3, ssc-mir-150-1, ssc-mir-150-2, mmu-let-7k, ssc-mir-378b, mmu-mir-9b-2, mmu-mir-9b-1, mmu-mir-9b-3
We found 13 adipogenesis-promoting miRNAs (let-7、miR-9、miR-15a、miR-17、miR-21、miR-24、miR-30、miR-103、miR-107、miR-125b、miR-204、miR-210、and miR-378) target 860 lncRNA loci. [score:3]
We analyzed the relationship between the 343 identified lncRNAs with the 13 promoting adipogenesis miRNAs (let-7、miR-9、miR-15a、miR-17、miR-21、miR-24、miR-30、miR-103、miR-107、miR-125b、miR-204、miR-210、and miR-378) and five depressing adipogenesis miRNAs (miR-27, miR-150, miR-221, miR-222, and miR-326). [score:1]
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[+] score: 3
The microarray data showed that several microRNAs including let-7, miR-923, miR-202, miR-21 and miR-145 were highly expressed in the porcine testes (Table S2). [score:3]
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33
[+] score: 3
The let-7 family miRNAs were all highly expressed in RX_J and RX_Y libraries. [score:3]
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34
[+] score: 3
The let-7a and -7f miRNAs were ubiquitously expressed in both tissues and accounted for over 1.90% of the total reads. [score:3]
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[+] score: 3
Among them, ssc-let-7f, which is derived from the let-7 -family, and the ssc-miR-21 had the highest expression level, and this result is consistent with previous studies [19]. [score:3]
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36
[+] score: 3
Potential reference genes (let-7a, mir-17 and mir-26a for subcutaneous adipose tissue and mir-17 and mir-20 for abdominal adipose tissue, liver and skeletal muscle) were tested for stability using GeNorm [41] and NormFinder [42] algorithms and were all used as reference genes to normalize the expression of the individual samples in the qPCR experiment. [score:3]
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37
[+] score: 3
We found that 13 miRNAs (ssc-miR-10b, -21, -26a, -99a, -126-3p, -143-3p, -148a-3p, -199a-3p, -199b-3p, -let-7i, -let-7g, -let-7f, and -let-7a) were highly expressed in both subcutaneous and abdominal adipose tissues from deep sequencing. [score:3]
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38
[+] score: 3
12946) miR-16-5p Potential regulator of SMAD3 102 1.23E-24 −9.938 Li et al. 2015 (10.2174/1381612821666150909094712) miR-1-3p Unknown 99 5.58E-24 −9.767 n/a let-7a-5p Potential regulator of CCND1 and MYC 78 7.77E-20 −8.622 Ghanbari et al. 2015 (10.4137/BIC. [score:3]
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39
[+] score: 2
Additionally, let-7a can regulate the implantation potential of activated blastocysts[45]. [score:2]
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40
[+] score: 2
Chart was plotted in ascending order of miRNA identifiers (leftmost, let-7a-1; rightmost, miR-4339). [score:1]
Host miRNA let-7a and 17 mature viral miRNAs, except for prv-miR-3-5′ and prv-miR-4-3′, were detected using this approach. [score:1]
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41
[+] score: 2
The discovery of the regulatory miRNA let-7 in C. elegans in 2000 [10], with homologs in other species including humans, caused researchers to reconsider the idea that miRNAs may have a more widespread function within cells. [score:2]
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42
[+] score: 2
Overall, miR were lowly abundant throughout fetal development with the exception of let-7 and muscle specific miR-1 and miR-206. [score:2]
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43
[+] score: 1
Other miRNAs from this paper: ssc-mir-122, ssc-mir-135-1, ssc-mir-135-2, ssc-mir-148a, ssc-mir-19a, ssc-mir-20a, ssc-mir-224, ssc-mir-24-1, ssc-mir-323, ssc-mir-140, ssc-mir-183, ssc-mir-214, ssc-mir-27a, ssc-mir-325, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-136, ssc-mir-153, ssc-mir-18a, ssc-mir-186, ssc-mir-196a-2, ssc-mir-204, ssc-mir-21, bta-mir-18b, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-148a, bta-mir-18a, bta-mir-20a, bta-mir-21, bta-mir-221, bta-mir-27a, bta-mir-27b, bta-mir-107, bta-mir-140, bta-mir-20b, bta-mir-215, bta-let-7d, bta-mir-17, bta-mir-186, bta-mir-199b, bta-mir-210, bta-mir-214, bta-mir-450a-2, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-15a, bta-mir-19a, bta-mir-204, ssc-mir-15a, ssc-mir-17, ssc-mir-199b, ssc-mir-210, ssc-mir-221, bta-mir-101-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-135a-2, bta-mir-135a-1, bta-mir-135b, bta-mir-136, bta-mir-146b, bta-mir-153-1, bta-mir-153-2, bta-mir-183, bta-mir-24-1, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-224, bta-mir-323, ssc-mir-101-1, ssc-mir-101-2, ssc-mir-133a-1, ssc-mir-450a, ssc-mir-146b, ssc-mir-215, bta-mir-1343, bta-mir-2320, bta-mir-2326, bta-mir-2366, bta-mir-2411, bta-mir-2483, bta-mir-450a-1, ssc-let-7e, ssc-let-7g, ssc-mir-103-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-450c, ssc-mir-133a-2, ssc-let-7a-2, ssc-mir-18b, ssc-mir-1343, ssc-mir-2320, bta-mir-450b, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-2366-1, ssc-mir-2366-2, ssc-mir-2411, ssc-mir-2483
Several miRNAs such as let-7, miR-27 and miR-103 are reported to perform very important functions in adipogenesis [33]– [35]. [score:1]
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44
[+] score: 1
Of these miRNAs, six (miR-133a-1/-2-3p, let-7a-1/-2-5p, miR-27b-3p, miR-26a-5p, miR-1-3p, and let-7f-1/-2-5p) were shared by all five stages and were closely related to myogenesis, cell growth, myocyte proliferation, and cell apoptosis. [score:1]
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45
[+] score: 1
Matching of the miRNA’s nt 13–17 can compensate for a single-nucleotide bulge or mismatch in the seed region, as illustrated by the experimentally validated let-7 sites in LIN41 [34] and the miR-196 site in HOXB8 [35]. [score:1]
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46
[+] score: 1
Luo et al. previously performed a sensitivity test of the microarray using the artificially transcribed miRNA of let-7a to hybridize to the let-7 probe set (let-7a to let-7g, let7-i). [score:1]
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47
[+] score: 1
Furthermore, miRNAs such as let-7 and members of the miR-290 cluster influence the early stages of gametogenesis, namely, primordial germ cell specification and migration [12], [13]. [score:1]
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[+] score: 1
Several miRNAs previously reported to be involved in immune response such as miR-21, miR-125a, miR-99b, miR-146a and let-7 families were identified. [score:1]
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49
[+] score: 1
After evaluation of its stability by geNorm v3.5 algorithm [36], five miRNAs (ssc-miR-26a, ssc-let-7a, ssc-miR-103, ssc-miR-17-5p and ssc-miR-16-5p) were used as reference to normalize expression [37]. [score:1]
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50
[+] score: 1
569 AMP miR-10, miR-126, let-7, miR-27, miR-450 9.860E-05–5.804E-04 0.789–0.730 ADP miR-15, miR-885, miR-322, miR-450, miR-338 1.316E-04–4.540E-03 0.781–0.636 ATP miR-15, miR-450, miR-210, miR-885, miR-451 4.811E-04–9.562E-03 0.737–0.593 Correlations between gene expression derived from post quality-filtered 17,820 mRNA probes and each phenotypic- trait were calculated for both Duroc and PiNN pigs. [score:1]
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