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7 publications mentioning sja-mir-2d

Open access articles that are associated with the species Schistosoma japonicum and mention the gene name mir-2d. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 12
miR-1b, miR-61 and miR-281 were highly expressed in males whereas miR-8447, miR-2f, mir-8437, miR-31, bantam, miR-2c, miR-2d, miR-71b, miR-36b and miR-755 were highly expressed in females. [score:5]
1005423.g003 Fig 3Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Furthermore, co-transfection in these experiments with antisense miR -mimics (miR-2 and let-7a) led to de-repression of luciferase (Fig 3A and 3B). [score:1]
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2
[+] score: 12
Interestingly, these five highly expressed miRNAs, miR-71b, miR-2a, miRNA-2e-5p, miRNA-2e-3p and miR-2f, were clustered in tandem at the same genomic locus (CNUS0000011792.1) (Figure 4A), whilst other miR-71 and miR-2 family members, miR-71a, miR-2d, miR-2b, and miR-2c, were clustered at another genomic locus (CNUS0000007682.1) in an inverted orientation (Figure 4B). [score:3]
In addition, both miR-71 and some miR-2 family members in tandem are found to be clustered in a reversal direction mo del on two genomic loci, and two pairs of novel S. japonicum miRNAs were derived from sense and antisense DNA strands at the same genomic loci. [score:2]
Although it is still not known how these Schistosoma miRNAs are evolutionarily related to each other, the observation that both the miR-71and miR-2 families lie within the same genomic clusters presents a mo del for the genesis of novel miRNAs, and implies that these genes exert their effects on worm development in a synergistic manner. [score:2]
Moreover, schistosomes also share some known miRNAs, such as bantam, miR-36, miR-60, miR-2 and miR-71 with Arthropoda and/or Nematoda animals. [score:1]
It should be pointed out that the cluster of miR-2 family members were found in silkworm (Bombyx mori), however, the combination of both miR-2 and miR-71 in the same clusters was not identified in other bilaterian animals. [score:1]
Some genes, such as insulin -induced gene 1 protein (INSIG-1), cytosolic thyroid hormone binding protein and thyroid hormone receptor interactor 10, possibly associated with the exploitation of host hormones, could be modulated by S. japonicum miR-31 and miR-2d. [score:1]
More interestingly, other miR-71 and miR-2 family members, miR-71a, miR-2d, miR-2b, and miR-2c, were clustered at another genomic locus (CNUS0000007682.1) in an inverted orientation. [score:1]
Here only two miRNA clusters identified by this study exhibited the similar genomic structure and components consisting of miR-71 and miR-2 family members. [score:1]
[1 to 20 of 8 sentences]
3
[+] score: 4
Five out of the 13 known miRNAs coming from one family named as sja-miR-2, including family members from miR-2a to miR-2e. [score:1]
Blue: the largest family members of miR-2. Two conserved miRNAs named as bantam and let-7 were found in both S. japonicum and S. mansoni miRNAs. [score:1]
Blue: the largest family members of miR-2. Two conserved miRNAs named as bantam and let-7 were found in both S. japonicum and S. mansoni miRNAs. [score:1]
Besides the miR-2 family, another two miRNAs, miR-71 and miR-71b-5p, were also found from one family, which is located on two different scaffolds named as SJC_S000054 and SJC_S000102. [score:1]
[1 to 20 of 4 sentences]
4
[+] score: 3
Moreover, the four novel miRNAs transcribed principally in male adults, including one miRNA (sha-miR-new_46) encoded on the same scaffold as the mir-71/mir-2 cluster (KL250488.1) with a CPM more than 20-times higher than that in the library derived from adult female worms, suggest male-specific roles for these miRNAs in gene regulation. [score:2]
These results are consistent with reports of a mir-71/mir-2 cluster duplication in S. mansoni [10, 11] and S. japonicum [14, 34] on the female-sex (W) chromosome and on one autosome (chromosome 5), and further support roles in sex-specific traits, sexual differentiation, pairing of adult worms and reproductive processes in schistosomes. [score:1]
[1 to 20 of 2 sentences]
5
[+] score: 3
We observed 2 miRNAs (mir-2 and mir-71) expressed in both life cycle stages tested, 7 in adult worms only (mir-4, mir-6, mir-9, mir-32, mir-125, mir-3, mir-5) and 5 in schistosomula only (mir-20, mir-18, mir-22, mir-26, Bantam). [score:3]
[1 to 20 of 1 sentences]
6
[+] score: 1
It also merit mention that the genomic arrangement of miRNAs, including miR-71a, the miR-2 family and sja-miR-novel-12 (miR-13), is conserved in S. mediterranea and S. mansoni. [score:1]
[1 to 20 of 1 sentences]
7
[+] score: 1
Furthermore, the 8 conserved miRNAs were identified as originating from 6 miRNA families, namely miR-124, miR-2 (miR-2b, miR-2e), bantam, miR-10, let-7 and miR-71 (miR-71, miR-71b). [score:1]
[1 to 20 of 1 sentences]