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8 publications mentioning hvu-MIR171

Open access articles that are associated with the species Hordeum vulgare and mention the gene name MIR171. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 191
Other miRNAs from this paper: hvu-MIR156a, hvu-MIR168, hvu-MIR156b
AK371946 was expressed in wildtype and showed reduced levels in OE171 plants (Figure 5A) suggesting that this was the main target down-regulated by miR171 over -expression in the shoot apex. [score:10]
Surprisingly, HvAP2L was up-regulated in OE171, suggesting that miR171 regulates HvAP2L expression independently from miR172. [score:7]
In Arabidopsis, miR171 acts mainly through the down-regulation of the SCL6-II/III/IV genes; over -expression of miR171 or loss of SCL6 function represses axillary meristem differentiation resulting in reduced shoot branching [30, 32], and eventually a complete developmental arrest under SD conditions [30]. [score:7]
As expected, HvSPL was down-regulated in OE171, reinforcing the hypothesis that miR171 acts at least partially through the up-regulation of miR156. [score:7]
This analysis identified 11 potential target ESTs predicted to be miR171-regulated by cleavage or translational repression using a mismatch score of 4.0 or less (Additional file 2). [score:6]
The fourth potential miR171 target (AK362896) encodes a protein of unknown function and is predicted to be regulated by translational repression. [score:6]
Arabidopsis plants over -expressing miR171c (OE171c) and the triple scl6 mutant show similar pleiotropic phenotypes, including altered shoot branching, plant height, chlorophyll accumulation, primary root elongation, flower structure, leaf shape and patterning, indicating that miR171 acts mainly by down -regulating SCL6 genes to control a wide range of developmental processes during shoot development [32]. [score:6]
First, the results, together with current knowledge from Arabidopsis, suggest that miR171 affects meristem maintenance and axillary meristem differentiation in barley through the down-regulation of HvSCL[28, 30], and consequently affects the expression of meristem specific genes such as the homologs of WUS and KN1 analysed in this study. [score:6]
Secondly, miR171 could repress vegetative phase transitions in barley through the upregulation of miR156, a known regulator of the transition from juvenile to adult phases across the angiosperms [2, 6- 9, 13, 45]. [score:5]
Overall, the data presented here suggest roles for miR171 and its targets in regulating shoot development in barley. [score:5]
However it is possible that other target genes with higher mismatch scores are affected in OE171 plants and that the other SCL6 genes are targeted by miR171 outside of the meristem tissues that we have focused on. [score:5]
In addition, the data show that miR171 over -expression alters the vegetative to reproductive phase transition by activating the miR156 pathway and repressing the expression of the TRD (THIRD OUTER GLUME) and HvPLA1 (Plastochron1) genes. [score:5]
The expression domains of the miR171 family members and the SCL6-II/III mRNAs overlap, suggesting a redundant function for both miRNA and target mRNAs [30- 32]. [score:5]
The strong expression of HvSCL in green spike and ovary overlaps with the highest miR171 abundance, at least at the tissue level, suggesting that miR171 acts to dampen HvSCL expression rather than restrict it [37]. [score:5]
To study the role of miR171 and its targets in barley, pri-miR171a was over-expressed under control of the maize ubiquitin promoter. [score:5]
Over -expression of miR171 affects expression of meristem identity genes suggesting a conservation of the role identified in Arabidopsis. [score:5]
In Arabidopsis, the miR171 family acts mainly through the down-regulation of three SCL6 genes (SCL6-II/III/IV)[32]. [score:4]
It is proposed that miR171 acts up-stream of miR156 and TRD which in turn may regulate Hv PLA1 expression as was observed for their orthologs in rice [44]. [score:4]
Of the target mRNAs with the lowest mismatch scores (1.5 or less), three (AK368048, AK371946 and AK364580) encode SCL6-like proteins and are all predicted to be regulated by miR171 cleavage. [score:4]
These apparent additional roles for miR171 and its targets in barley shoot development may represent an important evolutionary difference between monocot and dicot plants. [score:4]
In Arabidopsis miR171 represses differentiation of axillary meristems by repressing expression of SCARECROW-LIKE(SCL) transcription factors, however the role of miR171 has not been examined in other plants. [score:3]
In addition a delay in the transition to reproductive growth involving miR156 was observed which suggests that there are monocot specific functions for miR171 and its target genes. [score:3]
A mo del is proposed in which miR171 is an upstream regulator that coordinates the timing of shoot development in barley through three independent pathways. [score:3]
These observations suggest that barley miR171 can regulate axillary meristem development and the timing of the juvenile to adult phase transtition. [score:3]
In our subsequent analyses, T1 lines 1, 6 and 9 were used; these had similar phenotypes and expression of the mature miR171. [score:3]
The abundance of miR171 was examined in various tissues and it was found to be predominantly expressed in reproductive tissues (Figure 1A). [score:3]
Figure 1 Expression profile of hvu-miR171 and HvSCL. [score:3]
This last observation correlates with previous work in Arabidopsis reporting that TOE3 (an AP2-like gene containing a miR172 binding site) expression gradually increases along with miR171 abundance in the developing shoot [20]. [score:3]
These data correlate with previous observations in Arabidopsis where both miR171 and SCL target mRNAs are predominantly detected in the same tissues [33]. [score:3]
Figure 2 Expression of pri-miR171a and miR171 in OE171 transgenic lines. [score:3]
However, in contrast to the OE171 phenotype in barley, OE171 in Arabidopsis also promotes stem elongation [32], represses leaf initiation [30] and only slightly delays flowering [28], suggesting that the roles of miR171 and its targets evolved differently for these processes in barley and Arabidopsis. [score:3]
The OE171-3 T [0] plant had the highest miR171 expression and never flowered. [score:3]
Although studies in Arabidopsis have revealed important roles for miR171 and its SCL targets, their roles in monocot plants are unknown. [score:3]
As OE171 disrupts meristem function we extracted RNA from young inflorescence meristem tissues of T [1] OE171 transgenic lines and WT plants grown under short day conditions to quantify the abundance of miR171 and its targets. [score:3]
Mis -expression of miR171 in barley leads to pleiotropic phenotypes. [score:3]
To investigate the roles of mir171 and its target genes in a monocot, the Hvu pri-miR171a was over-expressed in barley (Hordeum vulgare L. cv. [score:3]
Our data suggest that some of the roles of miR171 and its target genes that have been determined in Arabidopsis are conserved in barley and that they have additional functions in barley including activation of the miR156 pathway. [score:3]
The T [o] miR171 over -expressing plants (OE171) showed a pleiotropic phenotype with altered shoot architecture and delayed flowering. [score:3]
These results suggest that miR171 coordinates vegetative phase changes in barley through the regulation of two potentially distinct pathways. [score:2]
This phenotype correlates with the observations here and suggests that miR171 regulates shoot branching through a conserved mechanism between monocots and dicots. [score:2]
miR171 is a well conserved miRNA family known to regulate members of the SCARECROW-LIKE (SCL) transcription factor family. [score:2]
In Arabidopsis, there are three MIR171 genes (a, b and c) which are predicted to regulate three SCL6 genes (SCLII, III, IV, also known as HAIRY MERISTEM (HAM) and LOST MERISTEMS (LOM), [28- 30]. [score:2]
Interestingly, OE171 and OE156 in Arabidopsis show opposite effects on leaf initiation [30, 32, 40], suggesting that the possible connection between the miR171 and miR156 pathways may be monocotyledon specific. [score:1]
Click here for file miR171 precursor and mature sequences. [score:1]
The degradome data for AK371946 had the greatest number of reads 2 bp upstream of the predicted cleavage site however RLM-5 [′] RACE showed that the predicted miR171 cleavage site was the most abundant (Additional file 3). [score:1]
Figure 5 Hvu-miR171-SCL molecular pathway. [score:1]
A corresponding increase in the abundance of mature miR171 was also detected in all the transgenic lines (Figure 2B). [score:1]
We searched degradome data generated from developing barley seed [36] for evidence of cleavage of these four mRNAs and found evidence for cleavage of the three SCL6-like mRNAs but not AK362896 (Additional file 3: AK364580 and AK368048 are identical at the miR171 cleavage site so cannot be distinguished in the degradome data). [score:1]
This effect may reflect a disorganization of the SAM and loss of maintenance of meristem cells, which correlates with miR171 function in Arabidopsis and could explain the reduction of shoot branching observed in barley OE171. [score:1]
Over -expression of miR171 results in an extended vegetative phase characterised by an increased number of leaves and the initiation of indeterminate axillary meristems instead of spikelet meristems. [score:1]
These data correlate with previous observations in Arabidopsis where miR171 mostly accumulates in reproductive organs [29, 31, 33]. [score:1]
All transgenic lines showed an over-accumulation of the pri-miR171a transcripts and mature miR171 sequences (Figure 5A, B). [score:1]
In barley two mature miR171 sequences (hvu-miR171a/b) have been identified [34] which differ by one central nucleotide. [score:1]
miR171 precursor and mature sequences. [score:1]
Thirdly, miR171 promotes vegetative traits in barley through a secondary pathway, independent from miR156 [40], that involves TRD and HvPLA1[42, 43]. [score:1]
These phenotypic similarities suggest that miR171 is linked to the miR156-miR172 pathway in barley. [score:1]
Evidence for miR171 cleavage of HvSCLs and HvSPL. [score:1]
Conserved molecular functions of miR171 in barley. [score:1]
There are nine rice, fourteen maize and four Brachypodium miR171 family members in miRBase, indicating that barley is likely to have additional miR171 genes. [score:1]
Click here for file Evidence for miR171 cleavage of HvSCLs and HvSPL. [score:1]
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2
[+] score: 64
Additionally, miR171 target gene was down-regulated in leaf but up-regulated in root upon boron stress (Fig. 2). [score:9]
In our study, miR169c, miR171, and miR399 were up-regulated in leaves whereas miR397, miR444b were down-regulated in roots after exposure to high B concentration. [score:7]
In barley, miR166 was up-regulated in leaves, but was down-regulated in roots; miR171 level was induced in leaves, but it was not affected in roots [19]. [score:7]
miR156, miR169c, miR171, miR171a, miR444a, miR444c, miR2023a were up-regulated while miR156d, miR397, miR408, miR1121, miR2014, miR5049, miR5141, miR5180, and miR5180a were down-regulated in leaf tissue upon boron stress. [score:7]
Similarly, miR171 was down-regulated but miR172 was up-regulated by cadmium exposure in Brassica napus [15]. [score:7]
In Medicago truncatula, miR169 and miR172 were up-regulated but miR171 and miR390 were down-regulated upon mercury exposure [16]. [score:7]
The miR169c and miR171 was determined to be 6-fold up-regulated and 2-fold up-regulated in leaves under boron stress, respectively. [score:7]
The expression levels of barley miRNAs and their targets were comparatively shown in Fig. 2. The miR159, miR164, miR166, miR171, and miR414 were induced in leaf, but were inhibited in root tissues exposed to boron stress. [score:7]
However, in response to Al [3+] treatment, miR171 was up-regulated in Medicago truncatula [14]. [score:4]
Several conserved miRNAs (such as miR160 and miR171) and non-conserved miR5141 were found abundantly in both libraries, but many others were detected with only a few in both libraries or could not be found in either library. [score:1]
Both conserved barley miRNAs (miR156, miR159, miR164, miR166, miR168, miR171, miR395 and miR396) and non-conserved barley miRNAs (miR1120 and miR5048) were detected. [score:1]
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3
[+] score: 37
In their study, they found that the expression of miR164, miR395, and miR156 was downregulated while miR159, miR167, and miR171 expression was upregulated in leaf tissues of wheat. [score:11]
The expression of miR171 targeting the Scarecrow-like transcription factor like (SCL-6) (Table 1) was found to be upregulated during drought stress in the leaves of barley while no change was detected in the root expression pattern for the same miRNA (Kantar et al. 2010). [score:10]
Interestingly, miR171 which targets the MYB family of transcription factors was found to be upregulated by salinity stress in both wheat and barley (Wang et al. 2014; Deng et al. 2015) (Table 2). [score:6]
Different tissue types may exhibit tissue-specific miRNA variation in the expression as is the case of miR171 from barley. [score:3]
Also, many miRNA families such as miR171 and miR393 showed induced expression in wheat in response to salinity stress (Gupta et al. 2014; Wang et al. 2014). [score:3]
Since both drought and salt stresses effect the osmotic balance of plant cells, miR171 might play a role in the regulation of the osmotic balance under such stress conditions. [score:2]
The manipulation of miR171 under salinity and drought stresses may provide improved osmotic protection to members of the Triticeae. [score:1]
durum, T. aestivum –Liu et al. 2015a, Ma et al. 2015, Akpinar and Budak 2016 miR168 T. aestivum –Gupta et al. 2014, Ma et al. 2015 miR169 H. vulgare –Hackenberg et al. 2014 miR171 T. turgidum ssp. [score:1]
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4
[+] score: 14
Three miRNAs (hvu-miR168-5p, hvu-miR5048a and hvu-miR444b) were down-regulated while two other miRNAs (hvu-miR171-5p and hvu-miR6213) were up-regulated under salinity stress. [score:7]
Among them, miR159, miR164, miR167 miR168, miR171, miR172 and miR393 have been demonstrated to be involved in signal transduction by targeting MYB transcription factor, F-box and Nodulation signaling pathway 2 proteins. [score:3]
Furthermore, hvu-miR171* showed a higher expression than hvu-miR171 under both salinity stress and normal condition. [score:3]
In addition, we have identified both mature and star sequences for two barley miRNAs (hvu-miR168 and hvu-miR171). [score:1]
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5
[+] score: 12
It was shown that SCLs are down-regulated by miR171 and that it is also able to repress the expression of the gene encoding protochlorophyllide oxidoreductase (POR; Ma et al., 2014). [score:6]
Arabidopsis miR171 -targeted scarecrow-like proteins bind to GT cis-elements and mediate gibberellin-regulated chlorophyll biosynthesis under light conditions. [score:4]
Recently, the link between SCL27, miR171 and chlorophyll a biosynthesis was reported in Arabidopsis (Ma et al., 2014). [score:1]
Interestingly, Laubinger et al. (2008) determined that the biogenesis of miR171 was under the control of CBC. [score:1]
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6
[+] score: 3
Transcription factor families comprise most of the highly conserved miRNA targets (see table 3) such as SBP family for miRNA 156, AP2 family for miR172, GRAS family for miR171, myb family for miR159, GRF family for miR396 and ARF family for miR160. [score:3]
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7
[+] score: 3
miRNAs from the same family can potentially have different functions depending on their expression profile, as suggested for members of the miR169 and miR171 families that differentially accumulate in response to abiotic stress in rice [31, 32]. [score:3]
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8
[+] score: 1
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR171a, osa-MIR393a, osa-MIR397a, osa-MIR397b, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR319b, osa-MIR166k, osa-MIR166l, osa-MIR168a, osa-MIR168b, osa-MIR169f, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR393b, osa-MIR172d, osa-MIR171i, osa-MIR166m, osa-MIR166j, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319b, zma-MIR166k, zma-MIR166j, zma-MIR168a, zma-MIR168b, zma-MIR169f, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, osa-MIR529a, tae-MIR159a, tae-MIR159b, tae-MIR171a, tae-MIR1120a, osa-MIR1430, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR166n, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR393b, zma-MIR393c, zma-MIR397a, zma-MIR397b, hvu-MIR156a, tae-MIR156, hvu-MIR159b, hvu-MIR159a, hvu-MIR166a, hvu-MIR168, hvu-MIR397a, tae-MIR171b, hvu-MIR1120, hvu-MIR166b, osa-MIR3981, hvu-MIR166c, tae-MIR1120b, tae-MIR397, tae-MIR1120c, hvu-MIR397b, hvu-MIR156b
The purity of RNA samples depleted of DNA traces was controlled by PCR amplification of the barley MIR171 gene. [score:1]
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