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2 publications mentioning tae-MIR5085

Open access articles that are associated with the species Triticum aestivum and mention the gene name MIR5085. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 23
Other miRNAs from this paper: osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR398a, osa-MIR398b, osa-MIR160e, osa-MIR160f, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR167j, osa-MIR437, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR818a, osa-MIR818b, osa-MIR818c, osa-MIR818d, osa-MIR818e, tae-MIR160, tae-MIR167a, tae-MIR1117, tae-MIR1118, tae-MIR1120a, tae-MIR1122a, tae-MIR1125, tae-MIR1127a, tae-MIR1128, tae-MIR1131, tae-MIR1133, tae-MIR1135, tae-MIR1136, tae-MIR1139, osa-MIR169r, osa-MIR1436, osa-MIR1439, osa-MIR2118a, osa-MIR2118b, osa-MIR2118c, osa-MIR2118d, osa-MIR2118e, osa-MIR2118f, osa-MIR2118g, osa-MIR2118h, osa-MIR2118i, osa-MIR2118j, osa-MIR2118k, osa-MIR2118l, osa-MIR2118m, osa-MIR2118n, osa-MIR2118o, osa-MIR2118p, osa-MIR2118q, osa-MIR2118r, bdi-MIR167a, bdi-MIR1139, bdi-MIR1122, bdi-MIR437, bdi-MIR169b, bdi-MIR1127, bdi-MIR1135, osa-MIR395x, osa-MIR395y, tae-MIR167b, tae-MIR169, tae-MIR395a, tae-MIR395b, tae-MIR398, bdi-MIR5070, bdi-MIR169d, bdi-MIR169i, bdi-MIR395a, bdi-MIR169j, bdi-MIR160a, bdi-MIR395b, bdi-MIR167b, bdi-MIR160b, bdi-MIR167c, bdi-MIR169k, bdi-MIR160c, bdi-MIR167d, bdi-MIR169g, bdi-MIR160d, bdi-MIR160e, bdi-MIR169e, bdi-MIR398a, bdi-MIR169a, bdi-MIR169h, bdi-MIR169c, bdi-MIR395c, bdi-MIR5180b, bdi-MIR5175a, bdi-MIR5175b, bdi-MIR395d, bdi-MIR398b, bdi-MIR5180a, bdi-MIR169f, bdi-MIR395m, bdi-MIR395e, bdi-MIR395f, bdi-MIR395g, bdi-MIR395h, bdi-MIR395j, bdi-MIR395k, bdi-MIR395l, bdi-MIR395n, osa-MIR818f, bdi-MIR167e, bdi-MIR395o, bdi-MIR395p, bdi-MIR5049, bdi-MIR160f, bdi-MIR167f, bdi-MIR167g, bdi-MIR169l, bdi-MIR169m, bdi-MIR169n, bdi-MIR395q, bdi-MIR2118a, bdi-MIR2118b, tae-MIR1122b, tae-MIR1127b, tae-MIR1122c, tae-MIR167c, tae-MIR5175, tae-MIR1120b, tae-MIR1120c, tae-MIR6197, tae-MIR5049
Other miRNAs included in this study (miR169, miR5085, miR6220, miR2118) may also be expressed, under stress conditions, in other wheat tissues and/or at different developmental stages. [score:4]
In order to show expression of selected pre-miRNAs (pre-miR2118, pre-miR169, pre-miR5085, pre-miR6220, pre-miR5070), RT-PCR and qRT-PCR was performed using Chinese Spring cDNA. [score:3]
Coding regions of both 5DL specific pre-miRNAs (pre-miR5085, pre-miR169) were found to be located between the breakpoint of 5DL-7 (FL : 0.29) and the centromere (Figure 3). [score:1]
Quantification with real-time PCR using CS gDNA suggested that coding regions of the selected pre-miRNAs had variable copy number: pre-miR169, pre-miR5085 and pre-miR5070 were shown to have approximately 8.6, 2.2 and 1.5 fold more copies than pre-miR6220. [score:1]
PCR screening of pre-miRNA coding sequences in flow sorted 5D short and long chromosome arms (5DS and 5DL); Triticum aestivum L. cv Chinese Spring (CS) and nullitetrasomic lines (N5D-T5A and N5D-T5B) (A) pre-miR169 (B) pre-miR5085 (C) pre miR5070 (D) pre-miR6220 (E) pre-miR2118. [score:1]
Our experimental results supported our in silico predictions: 5DS was verified to harbour regions coding for pre-miR2118 and pre-miR5070, and 5DL was confirmeded to contain both of the above plus pre-miR6220, pre-miR5085 and miR169 coding regions. [score:1]
q-RT PCR (A) Five miRNA coding regions (pre-miR169, pre-miR5085, pre-miR6220 and pre-miR5070) in Triticum aestivum L. cv Chinese Spring (CS) B) Levels of non 5D-specific miR5070, detected by qRT-PCR in nullitetrasomic line (N5D-T5A) and Triticum aestivum L. cv Chinese Spring (CS). [score:1]
To experimentally validate 5D chromosome localization of selected pre-miRNAs (miR169, miR5085, miR2118, miR5070, miR6220), PCR screening was carried out using DNA from flow-sorted 5D chromosome arms. [score:1]
In this study, five pre-miRNA (miR169, miR5085, miR2118, miR6220, miR2118) coding sequences were verified to be located to the 5D chromosome (Figure 2). [score:1]
5DL specific pre-miRNA (miR169, miR5085) coding sequences were shown to be located between the centromer and the breakpoint present in 5DL-7 (FL : 0.29) deletion line (Figure 3). [score:1]
PCR screening of 5DL specific pre-miRNA coding sequences (A) pre-miR169 (B) pre-miR5085 in Triticum aestivum L. cv Chinese Spring (CS) deletion lines (5DS-2, 5DS-5, 5DL-5, 5DL7) (C) Fraction length values of deletion lines. [score:1]
miR2118 was shown to be located on both arms of the 5D chromosome (5D specific), while miR5085 and miR169 were found to be specific to the long arm (Figure 2). [score:1]
0069801.g002 Figure 2PCR screening of pre-miRNA coding sequences in flow sorted 5D short and long chromosome arms (5DS and 5DL); Triticum aestivum L. cv Chinese Spring (CS) and nullitetrasomic lines (N5D-T5A and N5D-T5B) (A) pre-miR169 (B) pre-miR5085 (C) pre miR5070 (D) pre-miR6220 (E) pre-miR2118. [score:1]
pre-miR169, pre-miR5085 and pre-miR2118 coding regions were found to be 5D chromosome-specific. [score:1]
0069801.g003 Figure 3PCR screening of 5DL specific pre-miRNA coding sequences (A) pre-miR169 (B) pre-miR5085 in Triticum aestivum L. cv Chinese Spring (CS) deletion lines (5DS-2, 5DS-5, 5DL-5, 5DL7) (C) Fraction length values of deletion lines. [score:1]
2.5 mM MgCl [2] (stock concentration : 25 mM) was used for the amplification of miR6220, miR5070 and miR2118 and this value was optimized to 2 mM and 3 mM for the miR5085 and miR169 amplicons. [score:1]
0069801.g004 Figure 4q-RT PCR (A) Five miRNA coding regions (pre-miR169, pre-miR5085, pre-miR6220 and pre-miR5070) in Triticum aestivum L. cv Chinese Spring (CS) B) Levels of non 5D-specific miR5070, detected by qRT-PCR in nullitetrasomic line (N5D-T5A) and Triticum aestivum L. cv Chinese Spring (CS). [score:1]
Three of these pre-miRNAs (miR169, miR5085, miR2118) were shown to be 5D specific (Figure 2). [score:1]
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[+] score: 5
Specific expression of tae-miR399 was found in the control sample, whereas tae-miR5085, tae-miR5200, and tae-miR6197-5p were specifically expressed in the drought sample. [score:5]
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