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8 publications mentioning ssc-mir-218b

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-218b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 142
Based on our results, we speculate that the α-(1, 2) fucosyltransferase 2 gene (FUT2) and Discs, large homolog 5 (DLG5) genes were the targets of down-regulated ssc-miR-218-3p, the MUC4 gene was the target of down-regulated ssc-miR-136, MyD88 was the target of up-regulated ssc-miR-499-5p, LBP and Toll-like receptor (TLR4) genes were the target of up-regulated ssc-miR-196b. [score:21]
Based on our results, we speculate that the α-(1, 2) fucosyltransferase 2 gene (FUT2) and Discs, large homolog 5 (DLG5) genes were the targets of down-regulated ssc-miR-218-3p, the MUC4 gene was the target of down-regulated ssc-miR-136, MyD88 was the target of up-regulated ssc-miR-499-5p, LBP and Toll-like receptor (TLR4) genes were the target of up-regulated ssc-miR-196b. [score:21]
Interestingly, RT-qPCR showed that ssc-miR-136 and ssc-miR-218-3p were significantly up-regulated in F18-sensitive piglets (P < 0.05), and ssc-miR-196b and ssc-miR-499-5p were significantly up-regulated in F18-resistant piglets (P < 0.05). [score:7]
Based on the expression correlation between miRNA and target genes analysis, we speculate that miR-218-3p targeting to DLG5, appears to be very promising candidate for miRNAs involved in response to E. coli F18 infection. [score:7]
They show that mir-218-3p is down-regulated in sensitive tissues by RT-PCR (Table 1), and DLG5 is also down-regulated in the same tissue (fig. 8), yet they assert that the two are strongly NEGATIVELY correlated (fig. 9). [score:7]
It seems that there is a fundamental discrepancy between sequencing data (fig. 5) which shows that mir-218 is UP-regulated in sensitive tissues and the RT-PCR data (Table 1) which shows a significant DOWN-regulation in the same tissue, thus not a validation but instead in opposition. [score:7]
Based on the expression correlation between miRNA and potential target mRNAs, we speculate that DLG5, potential target gene of miR-218-3p, probably acts as a novel marker of E. coli F18 resistance. [score:7]
In the present study, RT-qPCR showed that the expression of miR-136, miR-218-3p, miR-196b and miR-499-5p were significantly up- or down-regulated between F18-sensitive and -resistant piglets. [score:6]
*means the difference was significant in the test level of P < 0.05 and **means extremely significant in 0.01 level In this study, we analysed the correlation between expression of miR-218-3p and six target genes (Fig.   9). [score:5]
However, the expression level of the miR-218-3p had significant positive correlations with LBP and MyD88 expression (P < 0.05). [score:5]
Previous studies have shown that above miRNAs (miR-136, miR-218-3p, miR-196b and miR-499-5p) indeed play roles in the development and regulation of human disease [32– 35]. [score:5]
Fig. 9Correlation analysis of gene expression of miR-218-3p with six potential target mRNAs in intestinal tissue. [score:5]
The expression level of miR-218-3p in intestinal tissue had a very significant negative correlation with DLG5 (P < 0.01), but there were no significant correlations with FUT2, MUC4 and TLR4 expression. [score:5]
Interestingly, the expression of miR-218-3p in intestinal tissue had a very significant negative correlation with target DLG5 (P < 0.01). [score:5]
These studies will further our understanding of the mechanisms of miR-218-3p as well as miRNA -mediated genes in the regulation of Mershan weaning piglets resistance to E. coli F18 and lay a solid foundation for the breeding of disease resistance to E. coli in Chinese domestic pig breeds. [score:4]
Therefore, we speculated that miR-218-3p targeting DLG5 was likely to regulate the formation of the E. coli F18 receptor and maintain the intestinal mucosa integrity of epithelial cells. [score:4]
Stem-loop RT-qPCR showed that miR-136, miR-196b, miR-499-5p and miR-218-3p significantly expressed in intestinal tissue (p < 0.05). [score:3]
This paper suggests that several miRNAs, notably mir-218-3p, are involved in E. Coli resistance in Meishan piglets, via targeting DLG5 among others. [score:3]
These results suggested that DLG5 was probably an important target gene of miR-218-3p. [score:3]
Author’s response: In our manuscript, we performed a comparative miRNA sequencing of duodenal tissues between E. coli F18-resistant group and E. coli F18-sensitive group, and then we screened out some differential expression miRNAs including mir-218-3p. [score:3]
In our study, the expression level of miR-218-3p in intestinal tissue had a very significant negative correlation with DLG5 (P < 0.01). [score:3]
Meanwhile, we could further analyze the correlation of mir-218-3p and DLG5 expression. [score:3]
In conclusion, we initially identified miR-196b, miR-499-5p and miR-218-3p as candidate miRNAs involved in E. coli F18 infection by miRNAs sequencing and qRT-PCR validation. [score:1]
However, the comparison of miR-SEQ and qRT-PCR has been shown in Fig. 5. 7/The authors should ideally demonstrate that mir-218-3p and DLG5 are both expressed in gut epithelial cells, which is not a given since they carried out measurements on entire duodenal tissue. [score:1]
However, the comparison of miR-SEQ and qRT-PCR has been shown in Fig. 5. 7/The authors should ideally demonstrate that mir-218-3p and DLG5 are both expressed in gut epithelial cells, which is not a given since they carried out measurements on entire duodenal tissue. [score:1]
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2
[+] score: 14
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-18a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-21, hsa-mir-23a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-96, hsa-mir-98, hsa-mir-99a, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-99a, mmu-mir-127, mmu-mir-128-1, mmu-mir-136, mmu-mir-142a, mmu-mir-145a, mmu-mir-10b, mmu-mir-182, mmu-mir-183, mmu-mir-187, mmu-mir-193a, mmu-mir-195a, mmu-mir-200b, mmu-mir-206, mmu-mir-143, hsa-mir-139, hsa-mir-10b, hsa-mir-182, hsa-mir-183, hsa-mir-187, hsa-mir-210, hsa-mir-216a, hsa-mir-217, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-224, hsa-mir-200b, mmu-mir-302a, mmu-let-7d, mmu-mir-106a, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-128-1, hsa-mir-142, hsa-mir-143, hsa-mir-145, hsa-mir-127, hsa-mir-136, hsa-mir-193a, hsa-mir-195, hsa-mir-206, mmu-mir-19b-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-18a, mmu-mir-21a, mmu-mir-23a, mmu-mir-31, mmu-mir-92a-2, mmu-mir-96, mmu-mir-98, hsa-mir-200c, mmu-mir-17, mmu-mir-139, mmu-mir-200c, mmu-mir-210, mmu-mir-216a, mmu-mir-219a-1, mmu-mir-221, mmu-mir-222, mmu-mir-224, mmu-mir-19b-1, mmu-mir-92a-1, mmu-mir-128-2, hsa-mir-128-2, mmu-mir-217, hsa-mir-200a, hsa-mir-302a, hsa-mir-219a-2, mmu-mir-219a-2, hsa-mir-363, mmu-mir-363, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-371a, hsa-mir-18b, hsa-mir-20b, hsa-mir-452, mmu-mir-452, ssc-mir-106a, ssc-mir-145, ssc-mir-216-1, ssc-mir-217-1, ssc-mir-224, ssc-mir-23a, ssc-mir-183, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-128-1, ssc-mir-136, ssc-mir-139, ssc-mir-18a, ssc-mir-21, hsa-mir-146b, hsa-mir-493, hsa-mir-495, hsa-mir-497, hsa-mir-505, mmu-mir-20b, hsa-mir-92b, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, hsa-mir-671, mmu-mir-216b, mmu-mir-671, mmu-mir-497a, mmu-mir-495, mmu-mir-146b, mmu-mir-708, mmu-mir-505, mmu-mir-18b, mmu-mir-493, mmu-mir-92b, hsa-mir-708, hsa-mir-216b, hsa-mir-935, hsa-mir-302e, hsa-mir-302f, ssc-mir-17, ssc-mir-210, ssc-mir-221, mmu-mir-1839, ssc-mir-146b, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-128-2, ssc-mir-143, ssc-mir-10b, ssc-mir-23b, ssc-mir-193a, ssc-mir-99a, ssc-mir-98, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-142, ssc-mir-497, ssc-mir-195, ssc-mir-127, ssc-mir-222, ssc-mir-708, ssc-mir-935, ssc-mir-19b-2, ssc-mir-19b-1, ssc-mir-1839, ssc-mir-505, ssc-mir-363-1, hsa-mir-219b, hsa-mir-371b, ssc-let-7a-2, ssc-mir-18b, ssc-mir-187, ssc-mir-219a, mmu-mir-195b, mmu-mir-145b, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-31, ssc-mir-182, ssc-mir-216-2, ssc-mir-217-2, ssc-mir-363-2, ssc-mir-452, ssc-mir-493, ssc-mir-671, mmu-let-7k, ssc-mir-7138, mmu-mir-219b, mmu-mir-216c, mmu-mir-142b, mmu-mir-497b, mmu-mir-935, ssc-mir-9843, ssc-mir-371, ssc-mir-219b, ssc-mir-96, ssc-mir-200b
adj ssc-miR-371-5p 11.3640 6.94E-19 7.93E-18 ssc-miR-219b-3p 10.1953 2.42E-32 1.94E-30 ssc-miR-218b 5.3242 5.95E-18 5.95E-17 ssc-miR-92b-3p 3.2034 3.39E-17 3.01E-16 ssc-miR-7138-3p 2.0714 1.31E-02 1.59E-02 ssc-miR-219a 2.0675 1.31E-07 4.37E-07 ssc-miR-99a 1.4504 2.83E-06 8.09E-06 ssc-miR-128 1.1854 1.31E-05 3.49E-05To validate this differential miRNA expression pattern, we performed quantitative stem-loop RT-PCR to assess the expression of the three[35] selected hpiPSCs- specific miRNAs: ssc-miR-371-5p, ssc-miR-106a and ssc-miR-363, which were found to be more highly expressed in hpiPSCs (Fig 3B). [score:7]
adj ssc-miR-371-5p 11.3640 6.94E-19 7.93E-18 ssc-miR-219b-3p 10.1953 2.42E-32 1.94E-30 ssc-miR-218b 5.3242 5.95E-18 5.95E-17 ssc-miR-92b-3p 3.2034 3.39E-17 3.01E-16 ssc-miR-7138-3p 2.0714 1.31E-02 1.59E-02 ssc-miR-219a 2.0675 1.31E-07 4.37E-07 ssc-miR-99a 1.4504 2.83E-06 8.09E-06 ssc-miR-128 1.1854 1.31E-05 3.49E-05 To validate this differential miRNA expression pattern, we performed quantitative stem-loop RT-PCR to assess the expression of the three[35] selected hpiPSCs- specific miRNAs: ssc-miR-371-5p, ssc-miR-106a and ssc-miR-363, which were found to be more highly expressed in hpiPSCs (Fig 3B). [score:7]
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3
[+] score: 9
miR-218 was considered as one of tumor suppressive miRNAs, and involved in modulation of the nuclear factor-kappa B (NF-κB) signaling pathway by directly targeting the IKK-β (IκBs kinase) gene and in activation of the mTOR (mammalian target of rapamycin)-Akt signaling pathway targeting the mTOR component Rictor [57], [58]. [score:9]
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4
[+] score: 8
For example, hsa-miR-133a, hsa-miR-200b, hsa-miR-206, and hsa-miR-218 were considered as tooth tissue-specific miRNAs [4]; eight differentially expressed miRNAs were expressed during morphogenesis and seven were expressed in the incisor cervical loop containing the stem cell niche [1]; the three most highly expressed microRNAs in dental epithelium were identified as mmu-miR-24, mmu-miR-200c, and mmu-miR-205, while mmu-miR-199a-3p and mmu-miR-705 were found in dental mesenchyme [2]; and miR-200 was suggested to play an important role in the formation of incisor cervical loop during stem cell–fueled incisor growth [5]. [score:8]
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5
[+] score: 4
Among these DE miRNAs, 14 were co-expressed, including miR-218-5p and miR-34c. [score:3]
Five central node genes (CTNNA2, ITGB1, ITGA4, LIMS1 and COL6A2) and two miRNAs (miR218-5p and miR-221-5p) were detected in the integrated network. [score:1]
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6
[+] score: 3
Among the non-profile-shared miRNAs, there were 6 high expressed miRNAs (CN>1,000 counts): miR-99a-5p (11,178 counts), miR-10a-5p (3,570 counts), miR-133a (1,990 counts), miR-218b (1,887 counts), miR-9-3p (1,620 counts) and miR-129a (1,566 counts). [score:3]
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7
[+] score: 2
Using miRNA microarray analysis and RT-PCR, some researchers have found that miR-24, miR-31, miR-140, miR-141, miR-205, miR-200c, miR-875-5p, miR-455, miR-689, miR-711, and miR-720 may regulate tooth epithelial stem cell differentiation [6], [7]; others identified miR-133a, miR-200b, miR-206, and miR-218 as tooth-specific miRNAs, and that miR-141, miR-199b*, miR-200a, miR-200b, miR-200c, and miR-429 likely play a role in the renewal and differentiation of adult stem cells during stem cell-fueled incisor growth [8], [9]. [score:2]
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8
[+] score: 1
Among them, six microRNAs (ssc-miR-128, ssc-miR-186, ssc-miR-4332, ssc-miR-218-3p, ssc-miR-2320-5p and ssc-miR-150) were predicted to bind to the 3′UTR of WUH3 PRRSV genome. [score:1]
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