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2 publications mentioning hco-mir-5352

Open access articles that are associated with the species Haemonchus contortus and mention the gene name mir-5352. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 103
From the H. contortus transcript microarray and qRT-PCR data [5], the pattern of expression of each of the miRNAs in the hco-miR-5352 cluster was very similar: all four were expressed at low levels in the larval stages, rising dramatically in the adult male and female worms. [score:5]
Hierarchical clustering of the microarray expression data showed that the four miRNAs in the hco-miR-5352 cluster group together based on their enrichment in adult female worms, suggesting that they may be co-expressed (see life cycle stage microarray). [score:5]
0006056.g001 Fig 1Developmental expression of the Hco-miR-5352 cluster across the H. contortus life cycle as assessed by microarray. [score:4]
Developmental expression of the Hco-miR-5352 cluster across the H. contortus life cycle as assessed by microarray. [score:4]
Fig 3A presents the analysis of hco-miR-5352 expression in adult female whole worms, adult ES and adult gut samples. [score:3]
The miRNAs of the hco-miR-5352 cluster are highly abundant in both libraries and show similar rankings, with expression of hco-miR-5352 being the lowest of the four, followed by hco-miR-43. [score:3]
Minimal expression of the hco-miR-5352 cluster sequences was observed in L3 stages (both sheathed L3 and activated L3, which had been exsheathed and cultured in vitro), the L4 stage and in adult female gut tissue. [score:3]
Although none of the four miRNAs of the miR-5352 cluster were enriched in female gut tissue, it was notable that of the ten most abundant miRNAs present in either L4 EV-enriched or L4 EV -depleted, most were highly expressed in adult worm gut tissue. [score:3]
Neither Angiostrongylus costaricensis nor A. cantonensis contained any member of the hco-miR-5352 cluster Hco-miR-5352 can be detected in excretory-secretory (ES) products and in infected sheep tissuesAs the miR-5352 cluster appeared to be expressed only in worms that reside at mucosal surfaces, we next determined whether it may be secreted from the parasites and could be detected in parasite ES or in infected tissues. [score:3]
While it was not possible to quantify the hco-miR-5352 signal precisely, a constitutively expressed miRNA, hco-miR-50, could be amplified from all three samples. [score:3]
While some miRNAs were highly expressed in gut tissue, the four members of the hco-miR-5352 cluster were not, suggesting that they are unlikely to be gut-derived. [score:3]
As the miR-5352 cluster appeared to be expressed only in worms that reside at mucosal surfaces, we next determined whether it may be secreted from the parasites and could be detected in parasite ES or in infected tissues. [score:3]
As the miRNAs in the hco-miR-5352 cluster were enriched in adult female worms, but not expressed in the adult female gut tissue, we assayed the presence of hco-miR-5352 in eggs/L1 collected from in vitro cultured worms. [score:2]
The hco-miR-5352 cluster consists of four miRNA loci (hco-miR-61, hco-miR-5352, hco-miR-43 and hco-miR-5895) all oriented in the same direction and closely linked in the genome, spanning a region of only 422 bp. [score:2]
The hco-miR-5352 cluster is most abundant in female wormsThe hco-miR-5352 cluster consists of four miRNA loci (hco-miR-61, hco-miR-5352, hco-miR-43 and hco-miR-5895) all oriented in the same direction and closely linked in the genome, spanning a region of only 422 bp. [score:2]
Direct comparison of the miRNA sequences and their relative abundance in adult worms and their ES products showed that all four members of the miR-5352 cluster appeared in the top ten miRNAs in adult worm ES indicating that these are secreted in vitro. [score:2]
The developmental expression of the miRNAs in the hco-miR-5352 cluster was first investigated from a microarray containing all known H. contortus miRNAs. [score:2]
miR-5352 and miR-43 were identified from Toxocara canis, while Anisakis simplex contained only miR-5352. [score:1]
Multiple alignment of the Hco-miR-5352 and Hco-miR-43 precursor and mature sequence and Ascaris suum miR-5352 and miR-43 sequences against the A. suum genome scaffold (scaffold:AscSuum_1.0_submitted:Scaffold187:163878:164556:-1) and A. lumbricoides genome scaffold (A_lumbricoides_Ecuador_v1_5_4, scaffold ALUE_scaffold0000721). [score:1]
H. contortus ES contains multiple additional small RNA sequencesThe data presented above demonstrated that hco-miR-5352 could be detected in adult worm ES material using qRT-PCR. [score:1]
In addition to the clade III species, the mature miR-5352 sequence was also identified from the bovine lungworm Dictyocaulus viviparus. [score:1]
For abomasal tissue samples and particularly the lymph node, hco-miR-5352 could be detected at a greater level in infected relative to uninfected animals (P<0.0001), while hco-miR-5895 gave no signal from tissue from uninfected animals but was detected in infected tissue samples (P<0.001). [score:1]
S5 FigPhylogenetic tree of the Hco-miR-5352 cluster sequences obtained from BLAST analysis. [score:1]
Nematode species and locations of miR-5352 cluster homologues. [score:1]
miR-5352 could be detected in eggs/L1 by qRT-PCR at a similar level to that of the adult female (Fig 3B). [score:1]
RNA was extracted from concentrated ES and qRT-PCR carried out to detect miR-5352, with whole adult female H. contortus cDNA being used as a positive control. [score:1]
The miR-5352 cluster was shown to be present in an additional ten parasitic nematodes, nine of which were clade V species. [score:1]
A phylogenetic tree using the sequences of the miR-5352 cluster from WormBase ParaSite is shown in S5 Fig, demonstrating that the cluster in A. suum and D. viviparus is more distantly related to that in the other species. [score:1]
As the presence of the cluster correlates with the anatomical site in the host, it seemed plausible that some of the miRNAs in the miR-5352-cluster may be secreted and thus have the potential to interact with host cells. [score:1]
Alignment of the three T. circumcincta miR-5352 cluster sequences obtained from WormBase ParaSite. [score:1]
miRNAs shown in bold indicate those present in the Hco-miR-5352 cluster. [score:1]
On the other hand, hco-miR-5960-5p, hco-miR-45-3p, hco-miR-5352-3p, hco-miR-87a-3p and hco-miR-84a-5p are all enriched in the L4 EV -depleted libraries. [score:1]
Hco-miR-5352 can be detected in excretory-secretory (ES) products and in infected sheep tissues. [score:1]
The hco-miR-5352 cluster is conserved in clade V nematodesBLASTN searches of the WormBase ParaSite database (parasite. [score:1]
S1 FigAlignment of the three T. circumcincta miR-5352 cluster sequences obtained from WormBase ParaSite. [score:1]
S2 FigMultiple alignment of the Hco-miR-5352 and Hco-miR-43 precursor and mature sequence and Ascaris suum miR-5352 and miR-43 sequences against the A. suum genome scaffold (scaffold:AscSuum_1.0_submitted:Scaffold187:163878:164556:-1) and A. lumbricoides genome scaffold (A_lumbricoides_Ecuador_v1_5_4, scaffold ALUE_scaffold0000721). [score:1]
hco-miR-5352 cluster precursor and mature sequences, along with A. suum miR-43 and 5352 sequences, were aligned to both A. suum and Ascaris lumbricoides scaffolds that contained miR-5352. [score:1]
org) identified homologues of the miR-5352 cluster in an additional ten clade V nematode species: Teladorsagia circumcincta, Oesophagostomum dentatum, Nippostrongylus brasiliensis, Necator americanus, Strongylus vulgaris, Heligmosomoides polygyrus, Cylicostephanus goldi, Ancylostoma duodenale, Ancylostoma ceylanicum and Dictyocaulus viviparus. [score:1]
Phylogenetic tree of the Hco-miR-5352 cluster sequences obtained from BLAST analysis. [score:1]
None of these sequences had previously been reported as miRNAs and none are included in miRBase, with the exception of the Ascaris miR-5352 and miR-43 sequence s. The genome of this clade III species contained only miR-5352 and miR-43, based on current sequence data, as did T. canis, consistent with its close relationship to Ascaris. [score:1]
A multiple alignment of the cluster sequences from these clade V organisms along with the sequence of the hco-miR-5352 cluster is presented in Fig 2. The D. viviparus cluster sequence is detailed below. [score:1]
0006056.g004 Fig 4qRT-PCR to detect presence of A) Hco-miR-5352, B) Hco-miR-5895 and C) Hco-miR-5960 in two infected and two pathogen-free (uninfected) sheep from abomasal (abo) and draining lymph node (lymph) tissue. [score:1]
qRT-PCR showed that hco-miR-5352 could be detected in the ES products and female worm extract but not in gut tissue, consistent with microarray data. [score:1]
The hco-miR-5352 cluster is conserved in clade V nematodes. [score:1]
S3 FigMultiple alignment of the Dictyocaulus viviparus contig 497 with the precursor and mature sequences of the H. contortus Hco-miR-5352 cluster. [score:1]
0006056.g003 Fig 3(A) qRT-PCR amplification of Hco-miR-5352 in adult female whole worms, adult ES and adult gut tissue. [score:1]
Multiple alignment of the Dictyocaulus viviparus contig 497 with the precursor and mature sequences of the H. contortus Hco-miR-5352 cluster. [score:1]
hco-miR-5352 was of particular interest as comparative analysis showed that it was conserved in the gastro-intestinal nematode Ascaris suum but not in other clade III nematodes [5]. [score:1]
Asu-miR-5352 is the only other miRNA in miRBase designated as miR-5352 and the precursor miRNA shows 70% identity to the H. contortus sequence (S2 Fig) [19]. [score:1]
RNA folding of miR-5352 homologues in D. viviparus using Mfold (http://unafold. [score:1]
A common feature of all the nematodes that possess the miR-5352 cluster is that the adult parasites are not tissue dwelling but reside in the gastro-intestinal tract of the host, with the exception of the bovine lungworm, D. viviparus. [score:1]
The hco-miR-5352 cluster is most abundant in female worms. [score:1]
From the BLAST searches, multiple sequences containing the hco-miR-5352 cluster were identified from H. polygyrus and T. circumcincta (parasite. [score:1]
0006056.g002 Fig 2BLAST searches were used to identify the scaffolds in WormBase ParaSite that were most similar to the H. contortus miR-5352 cluster. [score:1]
The dominant miRNA from each hairpin was submitted to miRBase (3p for hco-miR-61, hco-miR-5352 and hco-miR-43 and the 5p for hco-miR-5895). [score:1]
Neither Angiostrongylus costaricensis nor A. cantonensis contained any member of the hco-miR-5352 cluster BLAST searches were used to identify the scaffolds in WormBase ParaSite that were most similar to the H. contortus miR-5352 cluster. [score:1]
S2 TableNematode species and locations of miR-5352 cluster homologues. [score:1]
Asu-miR-5352 was then used to screen for miR-5352 sequences in other clade III species, revealing their presence only in other ascarid parasites. [score:1]
The specific databases from which members of the miR-5352 cluster were identified, the scaffold number and position and the location of these parasites within the host is shown in S2 Table. [score:1]
From all the parasitic nematodes for which genome data is available, only worms from the sub-order Strongylida contained the complete hco-miR-5352 cluster, with the exception of the super -family Metastrongyloidea. [score:1]
Of the nematodes that possess the miR-5352 cluster, both H. polygyrus and T. circumcincta have multiple versions of the miR-5352 cluster. [score:1]
However, miR-5352 could be detected in eggs/hatched L1 of H. contortus collected from in vitro cultures at a similar level to that detected in the whole female worm, suggesting that the embryos or the gonad may be the major source of this miRNA in female worms and possibly also ES. [score:1]
Homologues of the hco-miR-5352 cluster were identified using BLASTN searches of WormBase ParaSite (http://parasite. [score:1]
To determine whether miRNAs of the miR-5352 cluster may be released during infection, tissues from the abomasum epithelium and draining lymph nodes were analysed for the presence of hco-miR-5352 and hco-miR-5895, as well as hco-miR-5960, a miRNA that, while not a cluster member, is abundantly secreted in vitro. [score:1]
Interestingly for hco-miR-5352, the signal from the draining lymph node in infected sheep was significantly higher than that from the abomasal tissue (P = 1.59E-05). [score:1]
hco-miR-5352 was identified in that study and was shown to belong to a cluster of four miRNAs that appeared to be closely linked in the H. contortus genome. [score:1]
The data presented above demonstrated that hco-miR-5352 could be detected in adult worm ES material using qRT-PCR. [score:1]
S4 FigRNA folding of miR-5352 homologues in D. viviparus using Mfold (http://unafold. [score:1]
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[+] score: 1
These are Bpa-mir-5360, -5361, - 5363, -5364, -5365a, -5365b, and -5366, and Hco-mir-5352. [score:1]
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