6 publications mentioning ppe-MIR171a

Open access articles that are associated with the species Prunus persica and mention the gene name MIR171a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

The expression of miR169 was induced in leaf but inhibited in root tissues after drought treatment, whereas the expression level of miR171 was induced in root but inhibited in root tissues under drought.

Up-regulation of any miRNA expression levels was considered a positive value while negative values indicate down-regulation For miR156, miR164, miR166, miR168, miR169, miR171 and miR395, the miRNA stem-loop reverse transcription reaction was performed in a volume of 10 µL containing 2, 20, and 200 ng of total RNA samples of leaf and root samples (1 µL), 0.5 µL 10 mM dNTP mix, 1 µL stem-loop RT primer (1 µM) and 7.5 µL nuclease free water.

Similarly, miR171 expression was up-regulated in leaves of barley while transcript level of miR171 were only increased in the root, but not in the leaf of peach in response to drought.

The target transcripts of Ppe-mir156, Ppe-mir166, Ppe-mir168, Ppe-mir169, Ppe-mir171, and Ppe-mir395 were obtained using psRNATarget (user-submitted transcripts and miRNA option) and BlastN algoritms.

qRT-PCR was also used for detection and quantification of predicted targets of six drougt-responsive miRNAs (miR156, miR164, miR166, miR169, miR171 and miR395).

The relative expression profile of miR156, miR164, miR168, miR171 and miR395 using qRT-PCR had a good correlation with deep sequencing.

Among them, drought responsive miRNAs (miR156, miR164, miR166, miR168, miR169, miR171, and miR395) were detected and their expression levels were measured by qRT-PCR.

2

Further analysis on genes involved in miR171-SCL mo del showed that SCL and PORC were up-regulated and down-regulated respectively, which was in accordance with this mo del.

Although previous reports showed that miRNA171 was up-regulated under light [57], our results found that miRNA171c was less expressed after UVB stimulation.

It is indicated that the UVB-responsive miRNA171 plays an important role in regulating the growth and development of plant.

According to these results, we presumed that the increase of chlorophyll content under UVB might be not only related with miR171-SCL mo del but also regulated by non miR171-SCL pathway like the regulation of CHLG.

3

The expression levels of miR171, miR168, miR408a, miR398 and miR408b were significantly upregulated in mesocarp in NAA -treated samples compared to the control fruits, whereas those of miR156, miR160, miR166, miR167, miR390, miR393, miR482, miR535 and miR2118 were downregulated following NAA treatment.

The results of real-time PCR experiments revealed the increased expression levels of miR171, miR168, miR408a, miR398 and miR408b, as well as the reduced expression levels of miR166, miR167, miR160, miR156, miR2118, miR535, miR390, miR482 and miR393 in the peach fruit after NAA treatment, respectively, suggesting the functional divergence of microRNAs in the regulation of fruit development.

4

Five of the miRNAs (miR171, miR166i, miR159e, miR845, and miR396e) were enriched in the white flowers of Maroussia.

5

Most of conserved families common to Arabidopsis and peach (miR156, miR159, miR160, miR164, miR166, miR171, miR172, miR319, miR390, miR395, and miR396) did not show significant size variation (Figure 4).

6

Among the miRNA families in peach, miR156, miR159, miR160, miR166, miR171, miR319, miR390 and miR396 showed a high conservation in plants, indicating that these 12 peach miRNA families are ancient.