![]() |
miRBase |
![]() |
![]() |
![]() 3 publications mentioning mes-MIR394bOpen access articles that are associated with the species Manihot esculenta and mention the gene name MIR394b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
|
1 |
![]()
Other miRNAs from this paper: mes-MIR159a, mes-MIR167a, mes-MIR168a, mes-MIR171a, mes-MIR172a, mes-MIR394a, mes-MIR399a, mes-MIR156a, mes-MIR156b, mes-MIR156c, mes-MIR156d, mes-MIR156e, mes-MIR156f, mes-MIR156g, mes-MIR156h, mes-MIR156i, mes-MIR156j, mes-MIR156k, mes-MIR159b, mes-MIR159c, mes-MIR159d, mes-MIR160a, mes-MIR160b, mes-MIR160c, mes-MIR160d, mes-MIR160e, mes-MIR160f, mes-MIR160g, mes-MIR160h, mes-MIR162, mes-MIR164a, mes-MIR164b, mes-MIR164c, mes-MIR164d, mes-MIR166a, mes-MIR166b, mes-MIR166c, mes-MIR166d, mes-MIR166e, mes-MIR166f, mes-MIR166g, mes-MIR166h, mes-MIR166i, mes-MIR166j, mes-MIR167b, mes-MIR167c, mes-MIR167d, mes-MIR167e, mes-MIR167f, mes-MIR167g, mes-MIR167h, mes-MIR169a, mes-MIR169b, mes-MIR169c, mes-MIR169d, mes-MIR169e, mes-MIR169f, mes-MIR169g, mes-MIR169h, mes-MIR169i, mes-MIR169j, mes-MIR169k, mes-MIR169l, mes-MIR169m, mes-MIR169n, mes-MIR169o, mes-MIR169p, mes-MIR169q, mes-MIR169r, mes-MIR169s, mes-MIR169t, mes-MIR169u, mes-MIR169v, mes-MIR169w, mes-MIR169x, mes-MIR169y, mes-MIR169z, mes-MIR169aa, mes-MIR169ab, mes-MIR169ac, mes-MIR171b, mes-MIR171c, mes-MIR171d, mes-MIR171e, mes-MIR171f, mes-MIR171g, mes-MIR171h, mes-MIR171i, mes-MIR171j, mes-MIR171k, mes-MIR172b, mes-MIR172c, mes-MIR172d, mes-MIR172e, mes-MIR172f, mes-MIR319a, mes-MIR319b, mes-MIR319c, mes-MIR319d, mes-MIR319e, mes-MIR319f, mes-MIR319g, mes-MIR319h, mes-MIR394c, mes-MIR396a, mes-MIR396b, mes-MIR396c, mes-MIR396d, mes-MIR396e, mes-MIR396f, mes-MIR397, mes-MIR399b, mes-MIR399c, mes-MIR399d, mes-MIR399e, mes-MIR399f, mes-MIR399g, mes-MIR477h, mes-MIR477i, mes-MIR477a, mes-MIR477b, mes-MIR477c, mes-MIR477d, mes-MIR477e, mes-MIR477f, mes-MIR477g, mes-MIR535a, mes-MIR535b, mes-MIR2950, mes-MIR171l, mes-MIR398, mes-MIR399h, mes-MIR477j, mes-MIR477k, mes-MIR535c, mes-MIR535d, mes-MIR169ad
Surprisingly, some of the miRNAs slicing their targets were confirmed, but being positively correlated with the targets; these miRNA:target pairs included miR156: SPL13b, miR169: NF-YA3 and miR394: APL2/F-box.
[score:7]
The second target, SPS2F, maintained a relatively stable expression level at the early stage, but then fell close to zero from 180 DAP to 240 DAP; this suggested that miR394 also negatively regulated SPS2F (Figure 3G).
[score:6]
Other miRNA targets that are functional genes included genes that encode the AGPase large subunit 2 (miR394), an F-box protein (miR394), RD19 (miR167), a member of the zinc finger family (miR172), IRX12 (miR397) and two disease resistance proteins (NBS-LRR class; miR396 and −30).
[score:5]
In addition, two other miRNAs (miR394 and miR399) targeted APL2 and three sugar and carbohydrate metabolism-related genes (sugar transporter, invertase and carbohydrate transmembrane transporter), which have not been reported in other species.
[score:3]
For example, miR394 targets both cassava4.1_021267m (APL2) and cassava4.1_000867m (sucrose phosphate synthase 2F, SPS2F), which are involved in starch biosynthesis.
[score:3]
miR394 sliced two targets at different sites with different efficiencies: two slicing events at the 10th nucleotide in the CR and seven events downstream of the CR for F-box mRNA; there were also four slicing events at the 2nd and 14th nucleotides in CR and 14 slicing events upstream or downstream of CR occurred for ADP-glucose pyrophosphorylase large subunit 2 (APL2) mRNA.
[score:3]
However, given that there was no negative/positive correlation between miR394 and APL2 on the basis of the RNA-seq data in roots among the three Manihot genotypes, the regulation might only have existed in a specific growth stage of cassava storage root.
[score:2]
In contrast, an increase in levels of miR394 at 180 DAP, followed by a sustained increase from 210 DAP to 240 DAP, suggested that APL2 was negatively regulated by miR394 at a later stage.
[score:2]
[1 to 20 of 8 sentences]
|
2 |
![]()
Other miRNAs from this paper: ath-MIR159a, ath-MIR160a, ath-MIR160b, ath-MIR160c, ath-MIR165a, ath-MIR165b, ath-MIR167a, ath-MIR167b, ath-MIR171a, ath-MIR159b, ath-MIR319a, ath-MIR319b, ath-MIR167d, ath-MIR171b, ath-MIR171c, ath-MIR390a, ath-MIR390b, ath-MIR393a, ath-MIR393b, ath-MIR394a, ath-MIR394b, ath-MIR395a, ath-MIR395b, ath-MIR395c, ath-MIR395d, ath-MIR395e, ath-MIR395f, ath-MIR397a, ath-MIR397b, ath-MIR398a, ath-MIR398b, ath-MIR398c, ath-MIR408, ath-MIR159c, ath-MIR319c, ath-MIR167c, mes-MIR159a, mes-MIR167a, mes-MIR171a, mes-MIR394a, mes-MIR408, mes-MIR159b, mes-MIR159c, mes-MIR159d, mes-MIR160a, mes-MIR160b, mes-MIR160c, mes-MIR160d, mes-MIR160e, mes-MIR160f, mes-MIR160g, mes-MIR160h, mes-MIR167b, mes-MIR167c, mes-MIR167d, mes-MIR167e, mes-MIR167f, mes-MIR167g, mes-MIR167h, mes-MIR171b, mes-MIR171c, mes-MIR171d, mes-MIR171e, mes-MIR171f, mes-MIR171g, mes-MIR171h, mes-MIR171i, mes-MIR171j, mes-MIR171k, mes-MIR319a, mes-MIR319b, mes-MIR319c, mes-MIR319d, mes-MIR319e, mes-MIR319f, mes-MIR319g, mes-MIR319h, mes-MIR390, mes-MIR393a, mes-MIR393b, mes-MIR393c, mes-MIR393d, mes-MIR394c, mes-MIR395a, mes-MIR395b, mes-MIR395c, mes-MIR395d, mes-MIR395e, mes-MIR397, mes-MIR482, mes-MIR535a, mes-MIR535b, mes-MIR171l, mes-MIR398, mes-MIR535c, mes-MIR535d
It was possible to confirm predicted target downregulation for other induced conserved miRNA families, including miR394, miR165 and miR171 which target an F-box family protein, an ATHB transcription factor and a scarecrow-like transcription factor, respectively (Figure 3).
[score:8]
In some of these cases (miR171, miRE, miR394, miR197b) target expression decreased at 4 days post-inoculation (dpi) and then increased at 8 dpi, but never reached higher expression values than those without inoculation.
[score:7]
[1 to 20 of 2 sentences]
|
3 |
![]()
Other miRNAs from this paper: mes-MIR168a, mes-MIR171a, mes-MIR394a, mes-MIR166b, mes-MIR169a, mes-MIR169b, mes-MIR169c, mes-MIR169d, mes-MIR169e, mes-MIR169f, mes-MIR169g, mes-MIR169h, mes-MIR169i, mes-MIR169j, mes-MIR169k, mes-MIR169l, mes-MIR169m, mes-MIR169n, mes-MIR169o, mes-MIR169p, mes-MIR169q, mes-MIR169r, mes-MIR169s, mes-MIR169t, mes-MIR169u, mes-MIR169v, mes-MIR169w, mes-MIR169x, mes-MIR169y, mes-MIR169z, mes-MIR169aa, mes-MIR169ab, mes-MIR169ac, mes-MIR171b, mes-MIR171c, mes-MIR171d, mes-MIR171e, mes-MIR171f, mes-MIR171g, mes-MIR171h, mes-MIR171i, mes-MIR171j, mes-MIR171k, mes-MIR319a, mes-MIR319b, mes-MIR319c, mes-MIR319d, mes-MIR319e, mes-MIR319f, mes-MIR319g, mes-MIR319h, mes-MIR390, mes-MIR394c, mes-MIR395a, mes-MIR395b, mes-MIR395c, mes-MIR395d, mes-MIR395e, mes-MIR477a, mes-MIR171l, mes-MIR390b, mes-MIR398, mes-MIR169ad
Figure 3MiRNA cleavage sites on target genes of miR395, miR394 and half-phased miR171d.
[score:3]
Take miRNA394, a conserved plant miRNA, as an example.
[score:1]
2, miR394b.
[score:1]
A similar phasing pattern was observed on pre-miRNA394 in Arabidopsis and rice (Fig. 1a).
[score:1]
Besides the major miR394, a second miRNA-like RNA adjacent to the major miRNA was arranged in phase on pre-miR394 in cassava and castor bean (Fig. 1a).
[score:1]
[1 to 20 of 5 sentences]
|