8 publications mentioning ppe-MIR393b

Open access articles that are associated with the species Prunus persica and mention the gene name MIR393b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

The expression levels of miR171, miR168, miR408a, miR398 and miR408b were significantly upregulated in mesocarp in NAA -treated samples compared to the control fruits, whereas those of miR156, miR160, miR166, miR167, miR390, miR393, miR482, miR535 and miR2118 were downregulated following NAA treatment.

The results of real-time PCR experiments revealed the increased expression levels of miR171, miR168, miR408a, miR398 and miR408b, as well as the reduced expression levels of miR166, miR167, miR160, miR156, miR2118, miR535, miR390, miR482 and miR393 in the peach fruit after NAA treatment, respectively, suggesting the functional divergence of microRNAs in the regulation of fruit development.

miR156 and miR172 particularly targeted the SPL family gene colorless non-ripening (CNR) and the ethylene-responsive transcription factor gene APETALA2a, respectively, whereas miR393 targeted the AFB homolog gene SlTIR1.

Vazquez F. miR393 and secondary siRNAs regulate expression of the TIR1/AFB2 auxin receptor clade and auxin-related development of Arabidopsis leavesPlant Physiol.

In addition, miR167 can degrade IAA-Ala Resistant 3 (IAR3) in Arabidopsis roots under osmotic stress [16], whereas miR393 regulates the expression of the auxin receptor TIR1 and three other closely related auxin signaling F-box proteins (AFB1, AFB2 and AFB3) [17].

miR393 regulates expression of the auxin receptor gene TIR1 and the F-box genes AFB1, AFB2 and AFB3 [38, 39].

The experimental verification results were consistent with our high throughput sequencing datasets and also with previous studies that demonstrated the critical roles of miR160, miR166, miR167, miR390 and miR393 and their targeted genes in auxin signaling pathways.

In the tomato fruit, miR156, miR172, miR393 and their targets were also detected [21].

miR393 targeted the AFB homolog gene SlTIR1 that was shown to stimulate the fruit set and leaf morphogenesis in the tomato [51].

We noted that miR156, miR172 and miR393 were also detected in tomatoes.

Furthermore, we found that some miRNAs were also identified in the fleshy fruit of the tomato, including miR156, miR172 and miR393 [21].

Among these miRNAs, miR160, miR166, miR167, miR390 and miR393 are known to play important roles in auxin signaling pathways [14, 16, 17, 18, 35, 36].

2

The ten most highly expressed miRNAs (miR156, miR157, miR159, miR164, miR167, miR172, miR393, miR396, miR414, miR2275, and miR5021) in buds and leaves are miRNAs regulating genes involved in flower and leaf development processes such as integument development, leaf morphogenesis, meristem initiation, maintenance, and growth, bilateral symmetry determination, organ morphogenesis, plant phase transition, shoot apical meristem identity, flower and fruit development, and plant architecture.

In silico expression analyses of miRNAs using DEGseq [25] identified 19 sequences belonging to eight conserved miRNA families (miR156, miR157, miR164, miR172, miR393, miR396, miR414, and miR2275) induced in winter buds versus leaves (Additional file 6: Table S6).

3

miR164, miR165, miR166, miR172 and miR393 exhibited a similar phloem-specific expression pattern, i. e., they were highly expressed only in phloem tissues, whereas their expression level was relatively undetectable or very weak in the leaves and fruit at different stages.

4

The expression level of miR159, miR169, miR393, miR397, miR398 and miR393 were only decreased in root under drought stress while the miR395 were only down-regulated in leaf in response to drought (Figure 4).

5

Further, miR393 that targets TIR1/AFB2 Auxin Receptor (TAAR) Clade, another class of key players for auxin signaling [45] was also found to target three TAAR homologs in peach.

6

Mir393 and miR393* target two entirely different gene families (TIR1 and SNARE) both involved in pathogen resistance of host plant.

As an example, miR393* accumulates at a high level during infection of P. syringae in Arabidopsis leaves and promotes plant resistance to bacterial infection.

7

It was notable that the majority of the tested miRNAs were more abundant in flowers than in young leaves and stems, which suggests that these miRNAs might play important roles in peach flower development, whereas miR393 and miRC16 mainly accumulated in young leaves.

In addition, 11 miRNA families (miR162, miR164, miR167, miR168, miR169, miR172, miR393, miR394, miR397, miR399 and miR827) shared a high conservation in both dicotyledons and monocotyledons.

8

Previous reports have shown that miRNA induction was involved in regulating auxin signaling via miR160, miR167 and miR393 thus, becoming an important strategy for photomorphism in plants [23].