6 publications mentioning ppe-MIR396a

Open access articles that are associated with the species Prunus persica and mention the gene name MIR396a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

The ten most highly expressed miRNAs (miR156, miR157, miR159, miR164, miR167, miR172, miR393, miR396, miR414, miR2275, and miR5021) in buds and leaves are miRNAs regulating genes involved in flower and leaf development processes such as integument development, leaf morphogenesis, meristem initiation, maintenance, and growth, bilateral symmetry determination, organ morphogenesis, plant phase transition, shoot apical meristem identity, flower and fruit development, and plant architecture.

Members of four families (miR5021, miR164, miR414, miR396, miR2919) target genes encoding proteins involved in dormancy such as embryo-defective or maternal effect embryo arrest 14 (MEE14).

In silico expression analyses of miRNAs using DEGseq [25] identified 19 sequences belonging to eight conserved miRNA families (miR156, miR157, miR164, miR172, miR393, miR396, miR414, and miR2275) induced in winter buds versus leaves (Additional file 6: Table S6).

miR156, miR159, miR166, miR172, miR390, miR396, and miR5021 are the most expressed families in bud tissues.

Most of conserved families common to Arabidopsis and peach (miR156, miR159, miR160, miR164, miR166, miR171, miR172, miR319, miR390, miR395, and miR396) did not show significant size variation (Figure 4).

2

For example, miR396 that is known to target a conserved family of growth -regulating factor genes also targeted a rho guanyl-nucleotide exchange factor that is involved with phytochrome signaling.

The miR396 target encodes a rho guanyl-nucleotide exchange factor and the two miR408 targets encode a selenium -binding protein and a D-type cyclin (Table 2).

More importantly, novel targets were also identified for at least two conserved miRNAs, i. e. miR396 and miR408.

The tissue-specific expression patterns were presented for the conserved miRNAs, miR160, miR167, miR169, miR319, miR390 and miR396, and the less-conserved miRNAs, miR828, miR858 and miR2118 (Figure 2b).

3

As the expression levels of miR159, miR396 and miR397 were down-regulated in peach after treatment, this finding is inconsistent with previous reports suggesting that expression of these miRNAs was up-regulated in both A. thaliana and P. euphratica [42], [68].

4

The largest number of targets was shown by miR156, miR172 and miR396, with 25, 21 and 22, respectively.

Among the miRNA families in peach, miR156, miR159, miR160, miR166, miR171, miR319, miR390 and miR396 showed a high conservation in plants, indicating that these 12 peach miRNA families are ancient.

The abundance of miRNA families also varied drastically: miR157, miR166 and miR156 were most frequently represented in the library, with 154,908, 79,863 and 73,043 reads, whereas miR172, miR167, miR168 and miR396 were moderately abundant in the library with 6,411, 5,280, 4,373 and 2,500 copies.

5

For example, miR156, miR160, miR162, miR164, miR166, miR167, miR169, miR172, miR396, and miR397 are perfectly identical to the corresponding miRNAs in three other plant species (Arabidopsis thaliana, Populus trichocarpa, and Oryza sativa) (S3 Table).

6

Of these, ten (83%) were conserved peach miRNAs, including miR160, miR162, miR164d, miR166, miR168, miR396a, miR396b, miR398, miR408a and miR482 (Table S1).