11 publications mentioning bta-mir-2285cn

Open access articles that are associated with the species Bos taurus and mention the gene name mir-2285cn. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Co -expression network and pathway analysis revealed significant correlations between milk somatic cell count (SCC), an indicator trait for mastitis, and bta-miR-10a, bta-miR-2448, bta-miR-2284 family members and bta-miR-2285 family members [55].

A total of 77 miRNAs that included bta-miR-2422, bta-miR-135a, a bta-miR-2285 member, and several bta-miR-2284 family members showed expression differences in Staphylococcus aureus infected mammary glands compared to control groups [42].

2

In addition, the expression of 12 miRNA families including miR-130 (a, b), bta-miR-181 (a, b, c, d), bta-miR-199 (a-3p, a-5p, b, c), bta-miR-2285 (k, t), bta-miR-2411 (- 3p,-5p), bta-miR-2483 (- 3p,-5p), bta-miR-29 (a, b), bta-miR-339 (a, b), bta-miR-365 (- 3p,-5p), bta-miR-455 (- 3p,-5p), bta-miR-92, and bta-miR-99 (a,-5p, b) were differentially expressed between the granulosa cells of SF and DF (Table 1).

3

Of these 85 differentially expressed miRNAs, we detected six miRNA families, including miR-2285, miR-34, miR-192, miR-449, miR-200 families (Table S4).

4

The bta-mir-2285 family has over 40 members spanning the entire bovine genome, [20].

Seven of the other predicted miRNAs exhibited significant homology to two bovine miRNA families, bta-mir-2284 and bta-mir-2285.

Additionally 5 of the novel bovine miRNAs had significant homology with the bta-mir-2285 family.

5

These results were consistent with a previous report that mir-2285 and −2284 families had possibly over 40 members spanning the entire bovine genome [65].

In addition, 24 and 23 unique non-annotated sequences had significant homology with the bta-mir-2285 and bta-mir-2284 families, respectively (Additional file 4B).

6

The bta-miR-2284 and bta-miR-2285 families, for example, encode more than 100 mature miRNAs in the bovine genome but do not appear to have homologs in either human or mouse.

7

The largest miRNA family size identified was miR-2284, which consisted of 27 members; miR-2285, let-7, miR-30, and miR-376 possessed 22, 8, 6, and 5 members, respectively, whereas other miRNA families such as miR-107, miR-122, miR-140, and miR-1839 had only one member (Table S1).

8

However, the 21 members’ mir-2284, a ruminant-specific miRNA family [55], were not located on the same chromosome in the bovine and goat genomes, except for four precursors (mir-2284ab, mir-2285 k, mir-2285 l and mir-2285o).

9

Homology search in the miRBase database using the BLASTN method identified that 27 of the 31 novel miRNAs had 100% identity to known miRNAs in other species, one had a significant homology with the bta-miR-2285 family whereas three did not show significant homology to any other known miRNAs (Table 1).

10

Multiple reads confirmed the existence of four new members of the miR-2284 and miR-2285 families, encoded by five putative Ensembl microRNAs, predicted by alignment of microRNA sequences from miRBase to the bovine genome and evidence of potential stem-loop structures (Fig. 3A and Supplementary Table IV).

11

These analyses revealed 10 miRNAs which either were present in bovine but not in human (miR-1388-5p, miR-1388-3p, miR-2376, miR-2285 t, miR-3431 and miR-6529a, Fig. 5b) or were homologues of human miRNAs not identified previously in cow (miR-210-3p, miR-802, miR-4532 and miR-4792, Fig. 5c).