miRBase entry: dme-mir-2b-2

Stem-loop dme-mir-2b-2

Drosophila melanogaster dme-mir-2b-2 precursor miRNA
Gene family
MIPF0000049; mir-2

Caution, this is an AI generated summary based on literature. This may have errors. ?

dme-mir-2b is a member of the miR-2 family in Drosophila, and it shares the same mature sequence with dme-mir-2b-1 and dme-mir-2b-2, but is one nucleotide longer than the miRBase annotated sequences [PMC3268580]. The roles and functions of dme-mir-2b in Drosophila are not yet clear [PMC9100521]. Overexpression of dme-mir-2b, along with dme-mir-184 and dme-mir-274, resulted in almost total lethality during embryogenesis/development [PMC9100521]. Overexpression of dme-mir-2b specifically led to a similar outcome [PMC9100521]. Seven miRNAs, including dme-mir-2b, were found to be differentially expressed between long and short photoperiods [PMC9100521'>PMC9100521'>PMC9100521]. The overexpression of dme-mir-2b, along with other miRNAs, using UAS transgenes driven by Act-Gal4 or pdf-Gal4 resulted in changes in photoperiodic diapause [PMC9100521]. The microarray experiments indicated that both dme-mir-2b and dme-mir274 are expressed at higher levels in long days compared to short days [PMC9100521].

[PMC3268580] - Marco A. Mendoza-Mendoza et al. (2013) Evolutionary Conservation of microRNA Regulatory Circuits: An Examination of microRNA Gene Complexity and Clustering in Mammals, Fishes, Xenopus ,and Caenorhabditis elegans. PLoS ONE 8(6): e66415.
[PMC9100521] - Hui Zhang et al. (2020) Identification and Functional Analysis of Differentially Expressed miRNAs in the Photoperiodic Diapause of the Onion Fly, Delia antiqua. Frontiers in Physiology 11: 573.

Literature search
29 open access papers mention dme-mir-2b-2
(120 sentences)


532664 reads, 10189 reads per million, 49 experiments

       a          -         A   --uuu  cuu 
||||||| |||||||||| ||||||||| |||     ||    
agcgcag GAGGAGUUUC ACCGACACU Uac     cg   u
       C          G         A   uuauc  uau 

Annotation confidence High
Do you think this miRNA is real?
Stark et al. [2] have identified targets for miR-2 in Drosophila using computational prediction followed by experimental validation. miR-2 regulates the proapoptotic genes reaper, grim and sickle, suggesting that it may be involved in the control of apoptosis.

Genome context
chr2L: 19570182-19570264 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from dme-mir-2b-2
Name Accession Chromosome Start End Strand Confidence

Database links

Mature dme-miR-2b-3p

Accession MIMAT0000107
Description Drosophila melanogaster dme-miR-2b-3p mature miRNA
Evidence experimental
cloned [1,4], Northern [1,3], 454 [5-6], Illumina [6]
Database links
Predicted targets

Mature dme-miR-2b-2-5p

Accession MIMAT0020783
Description Drosophila melanogaster dme-miR-2b-2-5p mature miRNA
Evidence not_experimental
Database links


  1. PubMed ID: 11679670
    Identification of novel genes coding for small expressed RNAs
    "Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T"
    "Science (2001) 294:853-858

  2. PubMed ID: 12812784
    Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and broad-Complex gene activity
    "Sempere LF, Sokol NS, Dubrovsky EB, Berger EM, Ambros V"
    "Dev Biol (2003) 259:9-18

  3. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  4. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  5. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  6. PubMed ID: 14691535
    Identification of Drosophila MicroRNA targets
    "Stark A, Brennecke J, Russell RB, Cohen SM"
    "PLoS Biol (2003) 1:E60