Accession
MI0000120
Description
Drosophila melanogaster
dme-mir-2b-2 precursor miRNA
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
dme-mir-2b is a member of the miR-2 family in Drosophila, and it shares the same mature sequence with dme-mir-2b-1 and dme-mir-2b-2, but is one nucleotide longer than the miRBase annotated sequences [PMC3268580]. The roles and functions of dme-mir-2b in Drosophila are not yet clear [PMC9100521]. Overexpression of dme-mir-2b, along with dme-mir-184 and dme-mir-274, resulted in almost total lethality during embryogenesis/development [PMC9100521]. Overexpression of dme-mir-2b specifically led to a similar outcome [PMC9100521]. Seven miRNAs, including dme-mir-2b, were found to be differentially expressed between long and short photoperiods [PMC9100521'>PMC9100521'>PMC9100521]. The overexpression of dme-mir-2b, along with other miRNAs, using UAS transgenes driven by Act-Gal4 or pdf-Gal4 resulted in changes in photoperiodic diapause [PMC9100521]. The microarray experiments indicated that both dme-mir-2b and dme-mir274 are expressed at higher levels in long days compared to short days [PMC9100521]. References: [PMC3268580] - Marco A. Mendoza-Mendoza et al. (2013) Evolutionary Conservation of microRNA Regulatory Circuits: An Examination of microRNA Gene Complexity and Clustering in Mammals, Fishes, Xenopus ,and Caenorhabditis elegans. PLoS ONE 8(6): e66415. [PMC9100521] - Hui Zhang et al. (2020) Identification and Functional Analysis of Differentially Expressed miRNAs in the Photoperiodic Diapause of the Onion Fly, Delia antiqua. Frontiers in Physiology 11: 573.
Literature search
29 open access papers mention dme-mir-2b-2
(120 sentences)
(120 sentences)
Sequence
532664
reads,
10189
reads per million, 49 experiments
uugugucaUUCUUCAAAGUGGUUGUGAAAUGuuugccuuuuuaugccuauucaUAUCACAGCCAGCUUUGAGGAGCgacgcga
(((((((.(((((((((((((((((((.(((...((........)).....))).))))))))).)))))))))).)))))))
(((((((.(((((((((((((((((((.(((...((........)).....))).))))))))).)))))))))).)))))))
Structure
a - A --uuu cuu
uuguguc UUCUUCAAAG UGGUUGUGA AUG gc u
||||||| |||||||||| ||||||||| ||| ||
agcgcag GAGGAGUUUC ACCGACACU Uac cg u
C G A uuauc uau
Annotation confidence
High
Do you think this miRNA is real?
Comments
Stark et al. [2] have identified targets for miR-2 in Drosophila using computational prediction followed by experimental validation. miR-2 regulates the proapoptotic genes reaper, grim and sickle, suggesting that it may be involved in the control of apoptosis.
Genome context
chr2L: 19570182-19570264 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from dme-mir-2b-2
| Name | Accession | Chromosome | Start | End | Strand | Confidence |
|---|
Database links
Mature dme-miR-2b-3p
| Accession | MIMAT0000107 |
| Description | Drosophila melanogaster dme-miR-2b-3p mature miRNA |
| Sequence | 54 - UAUCACAGCCAGCUUUGAGGAGC - 76 |
| Evidence |
experimental
cloned [1,4], Northern [1,3], 454 [5-6], Illumina [6] |
| Database links |
|
| Predicted targets |
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Mature dme-miR-2b-2-5p
| Accession | MIMAT0020783 |
| Description | Drosophila melanogaster dme-miR-2b-2-5p mature miRNA |
| Sequence | 9 - UUCUUCAAAGUGGUUGUGAAAUG - 31 |
| Evidence | not_experimental |
| Database links |
|
References
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