Accession
MI0000303
Description
Caenorhabditis elegans
cel-mir-228 precursor miRNA mir-228
Gene
family?
family?
RF00843;
mir-228
Literature search
10 open access papers mention cel-mir-228
(28 sentences)
(28 sentences)
Sequence
3855577
reads,
18727
reads per million, 16 experiments
ccuuaucccguucgcAAUGGCACUGCAUGAAUUCACGGcuaugcauaacgacagaccGCGGAUCAUACGGUACCAUAGCggacggugaugagguuaau
(((((((((((((((.((((.((((.((((.(((.(((((.(........).)).))).))))))).)))).)))).)))))))).))))))).....
(((((((((((((((.((((.((((.((((.(((.(((((.(........).)).))).))))))).)))).)))).)))))))).))))))).....
Structure
----- - A C C A A - a gca
ccuuauc ccguucgc AUGG ACUG AUGA UUC CGG cu u u
||||||| |||||||| |||| |||| |||| ||| ||| || |
ggaguag ggcaggCG UACC UGGC UACU AGG Gcc ga a a
uaauu u A A A - C a c gca
Annotation confidence
High
Do you think this miRNA is real?
Comments
This precursor sequence was predicted by comparative computational approaches [1,2]. Northern blotting confirmed that the strand containing the predicted miR is predominantly expressed, and the 5' and 3' ends were confirmed later [4].
Genome context
chrIV: 5562025-5562122 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from cel-mir-228
| Name | Accession | Chromosome | Start | End | Strand | Confidence |
|---|
Database links
Mature cel-miR-228-5p
| Accession | MIMAT0000283 |
| Description | Caenorhabditis elegans cel-miR-228-5p mature miRNA |
| Sequence | 16 - AAUGGCACUGCAUGAAUUCACGG - 38 |
| Evidence |
experimental
cloned [1], Northern [1,3], PCR [3], 454 [4], Illumina [5,7], CLIPseq [6] |
| Database links |
|
| Predicted targets |
|
Mature cel-miR-228-3p
| Accession | MIMAT0020327 |
| Description | Caenorhabditis elegans cel-miR-228-3p mature miRNA |
| Sequence | 58 - GCGGAUCAUACGGUACCAUAGC - 79 |
| Evidence |
experimental
Illumina [7] |
| Database links |
|
References
|
