Stem-loop cel-mir-265

Accession

MI0000345

Description

Caenorhabditis elegans cel-mir-265 precursor miRNA

Literature search

2 open access papers mention cel-mir-265
(2 sentences)

Sequence

gguuugacuuacccccuccuuccgcuggccgccguuuuuauuaugaacuugaugugguuUGAGGGAGGAAGGGUGGUAUuugaacc
(((((((..((((((((((((((.(.((((((.((((.......)))).....)))))).).)))))).)))).)))).)))))))

Structure

       cu    -    -      g u      ----c    uu 
gguuuga  uacc cccu ccuucc c ggccgc     guuu  a
|||||||  |||| |||| |||||| | ||||||     ||||  u
ccaaguu  AUGG GGGA GGAGGG G uuggug     caag  u
       -U    U    A      A U      uaguu    ua 

Annotation confidence Low

Comments

This precursor sequence was predicted by comparative computational approaches. The excised miRNA sequence is predicted and the precise 5' or 3' end unknown. A PCR amplification protocol confirmed that the strand containing the predicted miR is predominantly expressed.

Genome context

chrIV: 12740443-12740528 [-]

Mature cel-miR-265

• Accession: MIMAT0000321
• Description: Caenorhabditis elegans cel-miR-265 mature miRNA
• Sequence: 60 - UGAGGGAGGAAGGGUGGUAU - 79
• Evidence: experimental ; PCR [1]

References

1. PubMed ID: 12769849
   Computational and experimental identification of C. elegans microRNAs
   "Grad Y, Aach J, Hayes GD, Reinhart BJ, Church GM, Ruvkun G, Kim J"
   "Mol Cell (2003) 11:1253-1263