Stem-loop cel-mir-265

Accession

MI0000345

Description

Caenorhabditis elegans cel-mir-265 precursor miRNA

Literature search

2 open access papers mention cel-mir-265
(2 sentences)

Sequence

gguuugacuuacccccuccuuccgcuggccgccguuuuuauuaugaacuugaugugguuUGAGGGAGGAAGGGUGGUAUuugaacc
(((((((..((((((((((((((.(.((((((.((((.......)))).....)))))).).)))))).)))).)))).)))))))

Structure

       cu    -    -      g u      ----c    uu 
gguuuga  uacc cccu ccuucc c ggccgc     guuu  a
|||||||  |||| |||| |||||| | ||||||     ||||  u
ccaaguu  AUGG GGGA GGAGGG G uuggug     caag  u
       -U    U    A      A U      uaguu    ua 

Annotation confidence Not enough data

Comments

This precursor sequence was predicted by comparative computational approaches. The excised miRNA sequence is predicted and the precise 5' or 3' end unknown. A PCR amplification protocol confirmed that the strand containing the predicted miR is predominantly expressed.

Genome context

chrIV: 12740443-12740528 [-]

Mature cel-miR-265

• Accession: MIMAT0000321
• Description: Caenorhabditis elegans cel-miR-265 mature miRNA
• Sequence: 60 - UGAGGGAGGAAGGGUGGUAU - 79
• Evidence: experimental ; PCR [1]

References

1. PubMed ID: 12769849
   Computational and experimental identification of C. elegans microRNAs
   "Grad Y, Aach J, Hayes GD, Reinhart BJ, Church GM, Ruvkun G, Kim J"
   "Mol Cell (2003) 11:1253-1263