Stem-loop cel-mir-269

Accession

MI0000349

Description

Caenorhabditis elegans cel-mir-269 precursor miRNA

Literature search

1 open access papers mention cel-mir-269
(7 sentences)

Sequence

2 reads, 0 reads per million, 2 experiments

gggcaaaaguugGGCAAGACUCUGGCAAAACUuggauuuaugcugcgccaaaguuuaacccaaguuugacccaacucuugucaaauuuuugucc
(((((((((((.(((((((....((..((((((((..(((.(((.......))).))).))))))))..))....))))))).)))))))))))

Structure

           g       CUCU  CA        au   u   gc 
gggcaaaaguu GGCAAGA    GG  AAACUugg  uua gcu  g
||||||||||| |||||||    ||  ||||||||  ||| |||  c
ccuguuuuuaa cuguucu    cc  uuugaacc  aau uga  c
           a       caac  ag        -c   u   aa 

Annotation confidence Low

Comments

This precursor sequence was predicted by comparative computational approaches. The excised miRNA sequence is predicted and the precise 5' or 3' end unknown. A PCR amplification protocol confirmed that the strand containing the predicted miR is predominantly expressed.

Genome context

chrIV: 273900-273993 [+]

Mature cel-miR-269

• Accession: MIMAT0000325
• Description: Caenorhabditis elegans cel-miR-269 mature miRNA
• Sequence: 13 - GGCAAGACUCUGGCAAAACU - 32
• Evidence: experimental ; PCR [1]

References

1. PubMed ID: 12769849
   Computational and experimental identification of C. elegans microRNAs
   "Grad Y, Aach J, Hayes GD, Reinhart BJ, Church GM, Ruvkun G, Kim J"
   "Mol Cell (2003) 11:1253-1263